QuEChERS: a sample preparation for extraction of carbaryl from rat feces

Carbamate compounds are an important group of cholinesterase inhibitors. There is a need for creating awareness regarding the risks of the inadequate carbamate use in the residential areas due to potential adverse human effects. Carbaryl is a commonly used pesticide worldwide. A simple, fast, and high-throughput method was developed employing liquid chromatography with fluorescence detector to determine carbaryl residues in rat feces. The extraction was performed by using a rapid, easy, cheap, effective, reliable, and safe (QuEChERS) method, using acetonitrile as the extracting solvent. The parameters for the performance of the extraction method were optimized, such as ratio of mass of sample per volume of extraction solvent, QuEChERS content, and cleanup columns. Linear response was obtained for all calibration curves (solvent and matrix-matched) over the established concentration range (5–500 μg/L) with a correlation coefficients higher than 0.999. The achieved recovery was 97.9% with relative standard deviation values of 1.1% (n = 4) at 167 μg/kg fortified concentration level and the limits of detection and quantification were 27.7 and 92.3 µg/kg, respectively.     

An analysis of the implementation of an environmental management system in a local public administration

The Environmental Management System (EMS) is commonly implemented in private firms. However, on the basis of a strengths, weaknesses, opportunities and threats (SWOT) analysis, our work analyzes the consequences of implementing an EMS within the context of local public administrations, particularly regarding the City Council of Ohanes in Almería (Spain). This is the first European corporation to implement an EMS according to the ISO 14001 Standard, certified by the Spanish Association of Normalization and Certification. Its analysis would be equivalent to the Shumpeterian "market innovator study", so that public administration "followers" can take advantage of the derived benefits and of minimizing the negative effects of such an experience. On the other hand, we show that the economic and environmental advantages derived from the EMS go beyond the activities that the City Council is in charge of. They have spillover effects that extend them to all economic activities in the municipality and these effects are expected to be increased in the medium and long-term perspective. In this paper, we compare the costs and benefits that the municipality obtains in two cases: the City Council implements the EMS or it does not implement it. The main objective of this article is to show the economic and environmental advantages obtained by a municipality when it is only the City Council who is implementing an EMS. It is logical to suppose that this case study can stimulate other municipalities to use this instrument, even if the economic and environmental characteristics of the municipality are different.     

Expedient Metrics to Describe Plant Community Change Across Gradients of Anthropogenic Influence

Human influence associated with land use may cause considerable biodiversity losses, namely in oceanic islands such as the Azores. Our goal was to identify plant indicator species across two gradients of increasing anthropogenic influence and management (arborescent and herbaceous communities) and determine similarity between plant communities of uncategorized vegetation plots to those in reference gradients using metrics derived from R programming. We intend to test and provide an expedient way to determine the conservation value of a given uncategorized vegetation plot based on the number of native, endemic, introduced, and invasive indicator species present. Using the metric IndVal, plant taxa with a significant indicator value for each community type in the two anthropogenic gradients were determined. A new metric, ComVal, was developed to assess the similarity of an uncategorized vegetation plot toward a reference community type, based on (i) the percentage of pre-defined indicator species from reference communities present in the vegetation plots, and (ii) the percentage of indicator species, specific to a given reference community type, present in the vegetation plot. Using a data resampling approach, the communities were randomly used as training or validation sets to classify vegetation plots based on ComVal. The percentage match with reference community types ranged from 77 to 100 % and from 79 to 100 %, for herbaceous and arborescent vegetation plots, respectively. Both IndVal and ComVal are part of a suite of useful tools characterizing plant communities and plant community change along gradients of anthropogenic influence without a priori knowledge of their biology and ecology.