The influence of lipid and lifestyle factors upon correlations between highly prevalent organochlorine compounds in patients with exocrine pancreatic cancer

We aimed to analyse the influence of cholesterol and triglycerides, and of tobacco, coffee and alcohol consumption upon correlations between serum concentrations of organochlorine compounds (OCs) in patients with exocrine pancreatic cancer (EPC). Incident cases of EPC diagnosed in eastern Spain were prospectively identified (N=144). OCs were analysed by high-resolution gas chromatography with electron-capture detection. A strong correlation was observed between hexachlorobenzene (HCB) and beta-hexachlorocyclohexane (beta-HCH) (Spearman's rho=0.758). beta-HCH showed rho>0.4 with p,p'-DDT, p,p'-DDE, PCB138 and PCB153 (all p<0.001). Some correlations among compounds were slightly affected by tobacco, coffee or alcohol consumption. We observed a striking diversity of correlation patterns by strata of cholesterol and triglycerides. Most correlations were higher in the lowest category of triglycerides than in the lowest category of cholesterol. Most coefficients above 0.7 were seen in the lowest category of triglycerides (e.g., OC pairs p,p'-DDT and HCB, p,p'-DDT and beta-HCH, p,p'-DDE and beta-HCH, or HCB and beta-HCH). Correlations among OCs may be stronger when concentrations of triglycerides are low than when they are high. This is compatible with a dilution in the early phases of cancer and with a concentration effect as triglycerides become lower in the advanced phases of the disease.     

Screening for Selected Human Pharmaceuticals and Cocaine in the Urban Streams of Manaus,Amazonas, Brazil

Pharmaceuticals and illicit drugs are contaminants that are generally ubiquitous in wastewater treatment plant effluents with their release into the environment being well understood in North America, Europe, and Asia. There is, however, less information on the release of human pharmaceuticals and illicit drugs from regions undergoing rapid land use, economical, and social changes, such as Brazil. This encompasses many areas in the tropical zone where releases of emerging contaminants may impact pristine, bio‐diversity rich ecosystems. In this study, the occurrence of human pharmaceuticals and the illicit drug cocaine was determined in the Rio Negro and two of its tributaries that receive large amounts of untreated sewage, the Igarapé Mindu and the Igarapé do 40, passing through the city of Manaus, Brazil. In addition to cocaine and its metabolite, benzoylecognine, propranolol, diclofenac, amitriptyline, carbamazepine, carbamazepine‐epoxide, citalopram, metoprolol, carisoprolol, and sertraline were all detected in two urban tributaries at low ng/l concentrations similar to those typically found in urban surface waters. Concentrations in the Rio Negro were typically lower than detection limits due to the large level of dilution, although traces of a range of pharmaceuticals were detected in the Rio Negro in proximity of the confluence of the urban streams. The data represent new information on the emissions of pharmaceuticals from a newly industrialized region of Brazil.     

In vitro toxic effects of heavy metals on fungal growth and phosphate-solubilising abilities of isolates obtained from Phragmites australis rhizosphere

In this research, we evaluated the toxic effect of metal ions on mycelial growth and phosphate-solubilising activity of soil-borne micromycetes isolated from the Phragmites australis rhizosphere using Pikovskaya-agar plates supplemented with four metal concentrations. The diameter growth rate (DGR) decreased as the metal concentration rise for all tested fungi. Trichoderma atroviride had the fastest growth rate (1.48?cm²?day^?1) and was the least susceptible to Al³⁺, Cd²⁺, Cr³⁺, Cu²⁺ and Pb²⁺ with a median effective concentration (MEC₅₀) of 12.19, 0.48, 4.51, 11.44 and 50.05?mM, respectively. Aspergillus japonicus was the most tolerant to Co²⁺, Ni²⁺ and Zn²⁺, with MEC₅₀ values of 3.36, 1.095 and 2.34?mM, respectively. Penicillium italicum was the most tolerant to Cr6⁺ (MEC₅₀?=?0.677?mM). The ability to solubilise phosphate remained, despite the decrease in the DGR, and P. italicum and Penicillium dipodomyicola had the highest Phosphate Solubilisation Indexes (PSIs) at 1.97 and 2.12, respectively. In particular, P. italicum recorded the highest PSI of all the studied isolates at 0.62?mM Cr³⁺ (PSI?=?4.74). A. japonicus and T. atroviride were the most tolerant isolates to all tested metals, which suggests that these isolates are promising candidates for further study with regard to mycoremediation and biofertilisation of metal-polluted soils.     

Estimated lead (Pb) exposures for a population of urban community gardeners

Urban community gardens provide affordable, locally grown, healthy foods and many other benefits. However, urban garden soils can contain lead (Pb) that may pose risks to human health. To help evaluate these risks, we measured Pb concentrations in soil, vegetables, and chicken eggs from New York City community gardens, and we asked gardeners about vegetable consumption and time spent in the garden. We then estimated Pb intakes deterministically and probabilistically for adult gardeners, children who spend time in the garden, and adult (non-gardener) household members. Most central tendency Pb intakes were below provisional total tolerable intake (PTTI) levels. High contact intakes generally exceeded PTTIs. Probabilistic estimates showed approximately 40 % of children and 10 % of gardeners exceeding PTTIs. Children’s exposure came primarily from dust ingestion and exposure to higher Pb soil between beds. Gardeners’ Pb intakes were comparable to children’s (in µg/day) but were dominated by vegetable consumption. Adult household members ate less garden-grown produce than gardeners and had the lowest Pb intakes. Our results suggest that healthy gardening practices to reduce Pb exposure in urban community gardens should focus on encouraging cultivation of lower Pb vegetables (i.e., fruits) for adult gardeners and on covering higher Pb non-bed soils accessible to young children. However, the common practice of replacement of root-zone bed soil with clean soil (e.g., in raised beds) has many benefits and should also continue to be encouraged.     

Attraction of entomopathogenic nematodes to sugarcane root volatiles under herbivory by a sap-sucking insect

Few systems have been described in which herbivore-induced root volatiles mediate attraction of entomopathogenic nematodes (EPNs), and they only concern root damage inflicted by chewing insects. EPNs, especially Heterorhabditis indica and Steinernema carpocapsae, are potential biological control agents of sugarcane spittlebug (Mahanarva fimbriolata) populations. Here, we investigated the response of these two species of EPNs to sugarcane root volatiles damaged by M. fimbriolata nymphs in a belowground six-arm olfactometer. We also examined changes on root volatile profile in response to herbivory of sugarcane spittlebug nymphs. Results showed that both EPN species did not discriminate between odors of undamaged sugarcane and moistened sand (blank). However, when EPNs were exposed to odors of spittlebug-damaged and undamaged sugarcane roots, both species significantly preferred odors of spittlebug-damaged roots. Headspace collection followed by GC–MS analyses showed no qualitative difference (total of 11 compounds) between volatile profiles of spittlebug-damaged and undamaged sugarcane roots. In contrast to the previous studies involving feeding by root chewing insects, our root volatile analysis did not reveal any up-regulation resulting from sugarcane spittlebug damage, but the down-regulation of the terpenes dihydromyrcenol and β-isomethyl ionone when compared with the profile of undamaged sugarcane roots. Here, we propose alternative explanations for the EPN attraction to spittlebug-damaged roots as it is unlikely that reduced concentrations of the volatiles play a role in this interaction. Further studies are necessary to determine the key compounds of the root volatile emission to enhance biological control efficacy with EPNs against M. fimbriolata in sugarcane.     

Smoothing of land use maps for trend and change detection in urbanization

Urban sprawl and its evolution over relatively short periods of time demands that we develop statistical tools to make best use of the routinely produced land use data from satellites. An efficient smoothing framework to estimate spatial patterns in binary raster maps derived from land use datasets is developed and presented in this paper. The framework is motivated by the need to model urbanization, specifically urban sprawl, and also its temporal evolution. We frame the problem as estimation of a probability of urbanization surface and use Bayesian P-splines as the tool of choice. Once such a probability map is produced, with associated uncertainty, we develop exploratory tools to identify regions of significant change across space and time. The proposal is used to study urbanisation and its development around the city of Bologna, Emilia Romagna, Italy, using land use data from the Cartography Archive of Emilia Romagna Region for the period 1976–2008.     

Formal modelling of predator preferences using molecular gut-content analysis

The literature on modelling a predator’s prey selection describes many intuitive indices, few of which have both reasonable statistical justification and tractable asymptotic properties. Here, we provide a simple model that meets both of these criteria, while extending previous work to include an array of data from multiple species and time points. Further, we apply the expectation–maximisation algorithm to compute estimates if exact counts of the number of prey species eaten in a particular time period are not observed. We conduct a simulation study to demonstrate the accuracy of our method, and illustrate the utility of the approach for field analysis of predation using a real data set, collected on wolf spiders using molecular gut-content analysis.     

Is aridity restricting deforestation and land uses in the South American Dry Chaco?

In this paper, we explored how aridity influences the regional deforestation and land-use patterns (i.e. crops/pastures) in South American Dry Chaco. To do this, we contrasted land use during last decade (2001–2012) with a spatially explicit aridity index, which we complemented with a crop water balance model. Land-use classifications were performed by considering the temporal variability of NDVI from MODIS satellites, showing that 40 and 60% of deforested land was assigned to crops and pastures, respectively. Results indicate that although the regional deforestation pattern was not associated with the aridity gradient, with drier areas similarly deforested as wetter areas, contrasting differences were observed in the use of this land, with crops mostly located (90%) in wetter areas and pastures evenly distributed across the whole aridity gradient. This research highlighted the strong effect of water limitations on the land-use option after deforestation and may help to set the basis for future land-use planning policies.     

Investigating stock structure and trophic relationships among island-associated dolphins in the oceanic waters of the North Atlantic using fatty acid and stable isotope analyses

Short-beaked common dolphins (Delphinus delphis) and Atlantic spotted dolphins (Stenella frontalis) are the two most abundant cetacean species in the oceanic waters of Madeira and the Azores. They are of similar size, occur in similar habitats and are regularly observed in mixed-species groups to forage together. Genetic analyses suggested that, within each species, dolphins ranging around both archipelagos belong to the same panmictic population. We tested the hypotheses that (1) within each species, individuals from the two archipelagos belong to a single ecological stock; (2) between species, common and spotted dolphins have distinct trophic niches; using fatty acid (FA) and stable isotope (SI) analyses. Fatty acids and stable isotopes were analysed from 86 blubber and 150 skin samples of free-ranging dolphins, respectively. Sex-related differences were not significant, except for common dolphin FA profiles. In S. frontalis, FA and SI differences between archipelagos suggested that individuals belonged to different ecological stocks, despite the existence of gene flow between the two archipelagos. In D. delphis, differences were more pronounced, but it was not possible to distinguish between stock structure and a seasonal effect, due to differential sampling periods in the Azores and Madeira. Inter-specific comparisons were restricted to the Azores where all samples were collected during summer. Differences in FA proportions, noticeably for FA of dietary origin, as well as in nitrogen SI profiles, confirmed that both species feed on distinct resources. This study emphasizes the need for an integrated approach including both genetic and biochemical analyses for stock assessment, especially in wide-ranging marine top predators.