肠道微生物群和水生毒理学: 环境健康的新概念

高通量测序技术极大地方便了深度考察不同种群中宿主相关微生物群的组成和功能。研究表明微生物在动物包括人类的健康和疾病发病中扮演着必要的角色。微生物已成为环境毒理学中新兴的重要研究主题。这是因为微生物在免疫系统中起着重要的交互的作用,同时在化学解毒中也有作用。污染物扰动肠道微生物,引起胃肠组织的病理生理变化,导致一系列系统效应,致使营养摄入变差和肠道发炎。本文检索了关于环境污染物对水生物种微生物影响的文献,重点关注了肠道微生物。我们强调了脊柱动物宿主中的一些已知的肠道上皮细胞的主要蛋白,这些均是化合物破坏的靶标,这些蛋白可以与微生物直接对话。我们提出了一个有害结局路径(adverse outcome pathway)的总体框架,将肠道生态失调作为有害效应终点事件的主要贡献因子。我们展示了两个案例研究,分别是(1)纳米材料;(2)碳氢化合物,我们参考了Deepwater Horizon港口的石油泄漏事件, 生态失调在案例中展示了微生物的考察如何改善有害结局的研究。最后,我们提出了一些策略以建立化合物诱导的肠道生态失调与有害结局的关联。我们通过实验建立了特定微生物与肠道生态失调的关联。对毒物与微生物关系的深入研究将成为改善动物及人类健康的重大突破。精选自Ondrej Adamovsky, Amanda N. Buerger, Alexis M. Wormington, Naomi Ector, Robert J. Griffitt, Joseph H. Bisesi Jr., Christopher J. Martyniuk. The gut microbiome and aquatic toxicology: An emerging concept for environmental health. Environmental Toxicology and Chemistry,2018,37:2758-2775.
详情请见 https://doi.org/10.1002/etc.4249
     

Colour-independent shape recognition of cryptic predators by bumblebees

Predators hunting for cryptic prey use search images, but how do prey search for cryptic predators? We address this question using the interaction between bumblebees and the colour-changing crab spider Misumena vatia which can camouflage itself on some flowers. In laboratory experiments, we exposed bumblebees to an array of flowers concealing robotic predators (a trapping mechanism combined with a 3D life-sized model of a crab spider or a circle). Groups of bees were trained to avoid either cryptic yellow spiders or yellow circles (equal area to the spiders) or remained predator naive. The bees were then exposed to a new patch of white flowers containing some cryptic predators (either white spiders, white circles or a mixture of both). We monitored individual foraging choices and used a 3D video tracking system to quantify the bees’ flight behaviour. The bees trained to avoid cryptic spiders, chose 40% fewer spider-harbouring flowers than expected by chance, but were indifferent to cryptic circles. They also aborted a higher proportion of landings on flowers harbouring spiders, ultimately feeding from half as many ‘dangerous’ flowers as naive bees. Previous encounters with cryptic spiders also influenced the flight behaviour of bees in the new flower patch. Experienced bees spent more time inspecting the flowers they chose to reject (both with and without concealed spiders) and scanned from side to side more in front of the flowers to facilitate predator detection. We conclude that bees disentangle shape from colour cues and thus can form a generalised search image for spider shapes, independent of colour.     

Support for maternal manipulation of developmental nutrition in a facultatively eusocial bee, <Emphasis Type=Italic>Megalopta genalis</Emphasis> (Halictidae)

Developmental maternal effects are a potentially important source of phenotypic variation, but they can be difficult to distinguish from other environmental factors. This is an important distinction within the context of social evolution, because if variation in offspring helping behavior is due to maternal manipulation, social selection may act on maternal phenotypes, as well as those of offspring. Factors correlated with social castes have been linked to variation in developmental nutrition, which might provide opportunity for females to manipulate the social behavior of their offspring. Megalopta genalis is a mass-provisioning facultatively eusocial sweat bee for which production of males and females in social and solitary nests is concurrent and asynchronous. Female offspring may become either gynes (reproductive dispersers) or workers (non-reproductive helpers). We predicted that if maternal manipulation plays a role in M. genalis caste determination, investment in daughters should vary more than for sons. The mass and protein content of pollen stores provided to female offspring varied significantly more than those of males, but volume and sugar content did not. Sugar content varied more among female eggs in social nests than in solitary nests. Provisions were larger, with higher nutrient content, for female eggs and in social nests. Adult females and males show different patterns of allometry, and their investment ratio ranged from 1.23 to 1.69. Adult body weight varied more for females than males, possibly reflecting increased variation in maternal investment in female offspring. These differences are consistent with a role for maternal manipulation in the social plasticity observed in M. genalis.     

Fragmentation of extracellular DNA by long-term exposure to radiation from uranium in aquatic environments

Persistent harmful scenarios associated with disposal of radioactive waste, high-background radiation areas and severe nuclear accidents are of great concern regarding consequences to both human health and the environment. Of particular concern is the extracellular DNA in aquatic environments contaminated by radiological substances. Strand breaks induced by radiation promote decrease in the transformation efficiency for extracellular DNA. The focus of this study is the quantification of DNA damage following long-term exposure (over one year) to low doses of natural uranium (an alpha particle emitter) to simulate natural conditions, since nothing is known about alpha radiation induced damage to extracellular DNA. A high-resolution Atomic Force Microscope was used to evaluate DNA fragments. Double-stranded plasmid pBS as a model for extracellular DNA was exposed to different amounts of natural uranium. It was demonstrated that low concentrations of U in water (50 to 150 ppm) produce appreciable numbers of double strand breaks, scaling with the square of the average doses. The importance of these findings for environment monitoring of radiological pollution is addressed.     

Partitioning gas tracer tests for measurement of water in municipal solid waste

A key component in the operation of almost all bioreactor landfills is the addition of water to maintain optimal moisture conditions. To determine how much water is needed and where to add it, in situ methods are required to measure water within solid waste. Existing technologies often result in measurements of unknown accuracy, because of the variability of solid waste materials and time-dependent changes in packing density, both of which influence most measurement methods. To overcome these problems, a new technology recently developed by hydrologists for measuring water in the vadose zone--the partitioning gas tracer test--was tested. In this technology, the transport behavior of two gas tracers within solid waste is used to measure the fraction of the void space filled with water. One tracer is conservative and does not react with solids or liquids, while a second tracer partitions into the water and is separated from the conservative tracer during transport. This technology was tested in four different solid waste packings and was capable of determining the volumetric water content to within 48% of actual values, with most measurement errors less than 15%. This technology and the factors that affect its applicability to landfills are discussed in this paper.     

Calcite-seeded crystallization of calcium phosphate for phosphorus recovery

For phosphorus recovery from wastewater, the present paper aims at understanding the crystallization of calcium phosphate by using calcites (Juraperle and Coccolith) as seeds from hard water. Synthetic hard water with 60 mgCl(-1) carbonate and 10 mgPl(-1) phosphate was prepared and used in the batch experiments of calcite-seeded crystallization. The solution composition was measured throughout the batch crystallization process, and the corresponding saturation indices with respect to the minerals were calculated with PHREEQC Program. The surface of the calcite seeds was observed and measured with environmental scanning electron microscopy (ESEM), Fourier transform infrared (FTIR) and BET method. The studies show that both calcites are effective seeds for the crystallization of calcium phosphate from hard water; the used calcites are more efficient than the original ones because newly formed crystals with calcium phosphate have covered their surfaces. The studies show that the calcite seeds can be used for phosphorus recovery from hard waters.     

The potential inhalation hazard posed by dioxin-contaminated soil

Conservative models were used to estimate the airborne concentrations of 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) vapor and particulates originating from soil containing 100 ppb TCDD. The upper-bound estimates were 3.25 pg/m³ of airborne TCDD vapor on-site and 0.51 pg/m³ for TCDD vapor 100 meters downwind. The TCDD air concentration on-site due to suspended particulate is estimated to be 1.4 pg/m³, based on a TSP level of 0.07 mg/m³. Assuming 70 years of continuous exposure to these concentrations, the upper-bound cancer risks determined from the Jury model were estimated to be 9.4 × 10⁻⁶ to 1.1 × 10⁻⁴ and 1.5 × 10⁻⁶ to 1.7 × 10⁻⁵ for inhalation of on- and off-site vapor, respectively, and 4.1 × 10⁻⁶ to 4.6 × 10⁻⁵ for dust inhalation. Since few sites have average soil concentrations as high as 100 ppb TCDD, this worst-case analysis indicates that inhalation will rarely, if ever, be a significant route of exposure to TCDD-contaminated soil. Experimental results support this claim and point to much lower risk estimates (8.4 × 10⁻⁹ to 9.9 × 10⁻⁸), suggesting that the parameters used in the Jury model are likely to overestimate the actual airborne levels of TCDD at contaminated sites.