AbstractIn the present study, ethanolic extract from
Heliopsis longipes roots and affinin/spilanthol against
Aspergillus parasiticus growth and aflatoxins production were studied in relation to the expression of
aflD and
aflR, two key genes of aflatoxins biosynthetic pathway. Phytochemical analysis of the ethanolic extract by GC-EIMS identified affinin/spilanthol (7.84?±?0.27?mg g
?1) as the most abundant compounds in
H. longipes roots. The antifungal and anti-aflatoxigenic assays showed that affinin/spilanthol at 300?µg mL
?1 produced the higher inhibition of radial growth (95%), as well as, the higher aflatoxins production inhibition (61%) in comparison to
H. longipes roots (87% and 48%, respectively). qRT-PCR revealed that the expression of
aflD and
aflR genes showed a higher downregulation in affinin/spilanthol at 300?µg mL
?1. The expression ratio of
alfD was suppressed by affinin/spilanthol in 79% and
aflR in 84%, while, a lower expression ratio suppressed by
H. longipes was obtained,
alfD (55%) and
aflR (59%). Affinin/spilanthol possesses higher antifungal and anti-aflatoxigenic activity against
A. parasiticus rather than
H. longipes roots, and this anti-aflaxotigenic activity occurring via downregulation of the
aflD and
aflR genes. Thus,
H. longipes roots and affinin/spilanthol can be considered potent antifungal agents against aflatoxigenic fungus, especially, affinin/spilanthol.
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