Endocrine response of the freshwater teleost, Sarotherodon mossambicus (Peters) to dimecron exposure

The endocrine response in a freshwater teleost, Sarotherodon mossambicus (Peters) under dimecron (an organophosphate pesticide) toxicity was investigated by estimating the serum levels of T3 (triiodothyronine), T4 (thyroxine), cortisol, prolactin and insulin in control and sub-lethal (0.001 ml l(-1)) dimecron-exposed fish for 1, 6, 12, 24h and 5 days. In control S. mossambicus, the serum levels of T3 ranged from 0.80+/-0.01 to 0.82+/-0.01 ng ml(-1); T4 from 2.20+/-0.01 to 2.25+/-0.01 microg dl(-1); cortisol from 8.30+/-0.03 to 8.34+/-0.01 microg dl(-1); prolactin from 1.50+/-0.01 to 1.54+/-0.01 microg ml(-1); insulin from 9.70+/-0.01 to 9.76+/-0.01 microU ml(-1) up to a maximum period of 5 days maintained in pollutant-free tap water. Exposure of fish to sub-lethal concentration of dimecron caused varying changes in the levels of serum hormones studied. Based on the results obtained, it was concluded that (i) the fish adaptively maintains a probable low metabolic rate, as indicated by the reduced levels of thyroid hormone (T3) as well as the glucocorticoid hormone (cortisol), which could be considered advantageous for the fish to indirectly reduce the toxic impact of the pesticide, (ii) the elevated levels of prolactin in the fish under pesticide stress is indicative of a possible hydromineral regulatory effect of the hormone (probably by influencing specific organs such as gills and kidney) under pesticide toxicity, (iii) the increased insulin level in the fish under pesticide stress is indicative of its role in favouring an adaptive tissue glycogenesis besides a possible increased lipogenesis to sequester the pesticide residue thereby reducing the toxic effect of the pesticide and (iv) the prolonged exposure of the fish (for 5 days) to sub-lethal dimecron appeared to exhibit a uniform recovery response in the different hormonal levels of the fish.     

Inter-laboratory study to improve the quality of the analysis of nutrients in rainwater chemistry

This paper describes the results of an inter-laboratory study conducted for the analysis of nutrients (nitrate, ammonium, phosphate, total nitrogen (TN), and total phosphorus (TP)) in natural rainwater. For this purpose, rainwater samples were collected and aggregated in Singapore and homogenized. These samples were immediately filtered through 0.45 μm membrane filters and autoclaved for 15 min at 80 °C in order to stabilize the nutrients. The homogeneity and the stability of nutrients were rigorously tested for a period of three months initially. Upon ensuring the homogeneity and stability, the samples were distributed to 15 different laboratories from various countries around the world (Australia, Brazil, India, Mauritius, Singapore, Slovenia, Spain, Taiwan, and USA). Almost all laboratories have reported the analytical results for nitrate whereas only 8 of the 15 laboratories reported results for other nutrients such as ammonium, phosphate, TN, and TP. The discrepancy was mainly due to the presence of these nutrients in low concentration levels (particularly ammonium ion and phosphate). Not all the laboratories were equipped with analytical capabilities to conduct the analysis of nutrients in low concentration levels. Further, the uncertainty associated with the analysis of TN and TP restricted the number of laboratories that could report their analytical data on nutrients. All 14 laboratories reported nitrate-nitrogen results which were in good agreement with each other (0.68 ± 0.07 mg l^?1). Similarly, the results of TN and TP were also comparable among at least 8 laboratories. This inter-laboratory study on the analysis of nutrients in natural rainwater, conducted for the first time, provided an opportunity to the participating laboratories to assess and improve their laboratory performance, thereby, improving the quality of their analytical data.     

Color removal of monoazo acid dye from aqueous solution by adsorption and chemical coagulation

This communication presents the results of an investigation conducted on color removal from a monoazo acid dye, C.I. Acid Red 88, by adsorption onto coal based sorbents like Lignite coal, Charfines and Bituminous coal and F-400 Activated carbon and by chemical coagulation employing Aluminum sulfate, Ferric chloride, Manganese sulfate, Manganese chloride and Barium chloride.     

A microscopic investigation of machining behavior in μED-milling process

The discussion presented in this work is about evaluation of physical behavior of μED-milling process based on channel shape, form and surface quality. μED-milling process is gaining lot of interest in recent times in micro manufacturing to generate complex shapes. Tool rotation and traverse which are not an inherent part of EDM process become important for μED-milling where it significantly influence the molten metal flow, debris flushing and redeposition. The effect of tool rotation not only disturbs the plasma but also affects the final shape and form of channel. Using scanning electron micrographs of μ-channel at different instant and conditions of machining, the physical nature of the process is understood and the results are presented. This study will provide a better understanding of the working phenomenon of μED-milling process.     

Residue level and manure application timing effects on runoff and sediment losses

There is growing interest in evaluating the effects of corn silage harvesting methods on erosion control. Increasing the silage cutting height will increase residue cover and could conceivably minimize off-site migration of sediments compared with conventional silage harvesting. The effects of residue level and manure application timing on runoff and sediment losses from no-till corn were examined. Treatments included conventional corn grain (G) and silage (SL) and nonconventional, high-cut (60-65 cm) silage (SH). Corn harvesting treatments were subjected to different manure application regimes: no manure (N) or surface application in fall (F) or spring (S). Simulated rainfall (76 mm/h; 1 h) was applied in spring and fall for two years (2002-2003), runoff from 2.0- x 1.5-m plots collected, and a subsample analyzed for sediment concentration and aggregate size distribution. Runoff volume was inversely related to residue cover. Manure addition to silage plots reduced spring runoff by 71 to 88%, attributable to an increase in soil organic matter content, compared with SH-N and SL-N. Differences in sediment concentration between SH and SL were not significant. For silage plots, spring-applied manure had the greatest influence on sediment export reducing it by 84 to 93% in spring runoff compared with corresponding N plots. Sediment loads were also 85 to 97% lower from SH-S compared with SL-N in all four seasons. Except for spring 2003, sediment export was lower from G compared with SL. The combination of manure and higher residue associated with high-cut silage often lowered sediment export compared with low-cut silage. Nearly identical aggregate size distributions were observed in sediments from SH and SL plots. High residue levels combined with spring-applied manure led to enrichment in the clay-sized fraction of runoff sediment. Recently applied manure and higher residue levels achieved by high-cutting silage can substantially lower sediment losses in spring runoff when soil is most susceptible to erosion.     

Improving the Chest Protection of Elderly Occupants in Frontal Crashes Using SMART Load Limiters

Objective: To determine whether varying the seat belt load limiter (SBL) according to crash and occupant characteristics could have real-world injury reduction benefits in frontal impacts and, if so, to quantify those benefits.

Methods: Real-world UK accident data were used to identify the target population of vehicle occupants and frontal crash scenarios where improved chest protection could be most beneficial. Generic baseline driver and front passenger numerical models using a 50th percentile dummy were developed with MADYMO software. Simulations were performed where the load limiter threshold was varied in selected frontal impact scenarios. For each SBL setting, restraint performance, dummy kinematics, and injury outcome were studied in 5 different frontal impact types. Thoracic injury predictions were converted into injury probability values using Abbreviated Injury Scale (AIS) 2+ age-dependent thoracic risk curves developed and validated based on a methodology proposed by Laituri et al. (2005). Real-world benefit was quantified using the predicted AIS 2+ risk and assuming that an appropriate adaptive system was fitted to all the cars in a real-world sample of recent frontal crashes involving European passenger cars.

Results: From the accident data sample the chest was the most frequently injured body region at an AIS 2+ level in frontal impacts (7% of front seat occupants). The proportion of older vehicle front seat occupants (>64 years) with AIS 2+ injury was also greater than the proportion of younger occupants. Additionally, older occupants were more likely to sustain seat belt–induced serious chest injury in low- and moderate-speed frontal crashes. In both front seating positions, the low SBL provided the best chest injury protection, without increasing the risk to other body regions. In severe impacts, the low SBL allowed the driver to move dangerously close to the steering wheel. Compared to the driver side, greater ride-down space on the passenger side gave a higher potential for using the low SBLs. When applying the AIS 2+ risk reduction findings to the weighted accident data sample, the risk of sustaining an AIS 2+ seat belt injury changed to 0.9, 4.9, and 8.1% for young, mid, and older occupants, respectively, from their actual injury risk of 1.3, 7.6, and 13.1%.

Conclusions: These results suggest the potential for improving the safety of older occupants with the development of smarter restraint systems. This is an important finding because the number of older users is expected to increase rapidly over the next 20 years. The greatest benefits were seen at lower crash severities. This is also important because most real-world crashes occur at lower speeds.     

Spectrophotometric determination of platinum(IV) in alloys, complexes, environmental, and pharmaceutical samples using 4-[N,N-(diethyl)amino] benzaldehyde thiosemicarbazone

4-[N,N-(Diethyl)amino] benzaldehyde thiosemicarbazone (DEABT) is proposed as an analytical reagent for the spectrophotometric determination of platinum(IV). The DEABT forms 1:2 yellow complex with Pt(IV), which is sparingly soluble in water and completely soluble in water–ethanol–DMF medium. The Pt(IV)–DEABT complex shows maximum absorbance at 405 nm. Beer’s law is valid up to 7.80 μg cm^???3, and optimum concentration range for the determination of platinum(IV) is 0.48–7.02 μg cm^???3. The molar absorptivity and Sandell’s sensitivity of the method are found to be 1.755 × 10⁴ dm³ mol^???1 cm^???1 and 0.0012 μg cm^???2, respectively. The relative error and coefficient of variation (n?=?6) for the method does not exceed ±0.43% and 0.35%, respectively. Since the method tolerates a number of metal ions commonly associated with platinum, it can be employed for the determination of platinum in environmental samples, pharmaceutical samples, alloys, catalysts, and complexes. The method is rapid as the Pt(IV)–DEABT complex is soluble in water–ethanol–DMF medium and not requiring any time consuming extraction method for the complex.     

Adsorption of ammonium ion by coconut shell-activated carbon from aqueous solution: kinetic, isotherm, and thermodynamic studies

Ammonium ions are one of the most encountered nitrogen species in polluted water bodies. High level of ammonium ion in aqueous solution imparts unpleasant taste and odor problems, which can interfere with the life of aquatics and human population when discharged. Many chemical methods are developed and being used for removal of ammonium ion from aqueous solution. Among various techniques, adsorption was found to be the most feasible and environmentally friendly with the use of natural-activated adsorbents. Hence, in this study, coconut shell-activated carbon (CSAC) was prepared and used for the removal of ammonium ion by adsorption techniques. Ammonium chloride (analytical grade) was purchased from Merck Chemicals for adsorption studies. The CSAC was used to adsorb ammonium ions under stirring at 100 rpm, using orbital shaker in batch experiments. The concentration of ammonium ion was estimated by ammonia distillate, using a Buchi distillation unit. The influence of process parameters such as pH, temperature, and contact time was studied for adsorption of ammonium ion, and kinetic, isotherm models were validated to understand the mechanism of adsorption of ammonium ion by CSAC. Thermodynamic properties such as ?G, ?H, and ?S were determined for the ammonium adsorption, using van't Hoff equation. Further, the adsorption of ammonium ion was confirmed through instrumental analyses such as SEM, XRD, and FTIR. The optimum conditions for the effective adsorption of ammonium ion onto CSAC were found to be pH 9.0, temperature 283 K, and contact time 120 min. The experimental data was best followed by pseudosecond order equation, and the adsorption isotherm model obeyed the Freundlich isotherm. This explains the ammonium ion adsorption onto CSAC which was a multilayer adsorption with intraparticle diffusion. Negative enthalpy confirmed that this adsorption process was exothermic. The instrumental analyses confirmed the adsorption of ammonium ion onto CSAC.     

Simple synthesis of hydrazones with quorum quenching activity at room temperature in water

Environmental Chemistry Letters - The hydrazone moiety is a major function in medicinal chemistry because of its wide range of pharmacological applications. Actual synthetic procedures usually...