Ectomycorrhizal colonization of loblolly pine seedlings during three growing seasons in response to ozone, acidic precipitation, and soil Mg status

A three-year study was initiated in 1987 to evaluate the impact of O3, acidic precipitation, and soil Mg on ectomycorrhizal colonization of loblolly pine (Pinus taeda L.) seedlings. Thirty-six open-top chambers equipped with a rainfall exclusion/addition system were utilized to administer three levels of O3 (subambient, ambient, or twice ambient) and two precipitation acidity levels (pH 3.8 or 5.2) to seedlings growing in 24-liter plastic pots containing soil having either 35 or 15 mg kg(-1) of exchangeable Mg. Seedlings exposed to the twice ambient O3 treatment exhibited smaller percentages of total ectomycorrhizal short roots at the end of each year of the study, but trends were statistically significant in 1989 only. Changes in number of specific ectomycorrhizal morphotypes in response to O3 were not consistent from year to year. Acidic precipitation treatments had no effect on number or percent of mycorrhizal short roots, and responses of two morphotypes to soil Mg treatments were probably due to differences in the soil environment rather than a result of changes in aboveground processes. Temporal shifts in morphotype frequencies were observed for seedlings in all treatments and indicate that mycorrhizal succession occurred during the study period.     

Study of controlled migration of cadmium and lead into foods from plastic utensils for children

Lead (Pb) is a highly neurotoxic chemical element known for reducing intelligence quotient (IQ) and promoting antisocial behavior in children and adolescents, while cadmium (Cd) is a carcinogenic bioaccumulative element. Both these metals are included in the priority pollutant list of the United States Environmental Protection Agency and in the WHO List of Chemicals of Major Public Health Concern, where contaminated foods and beverages are the most common pathways of exposure. The objective of this study was to determine total Cd and Pb levels in colored plastic utensils (cups, mugs, bowls, feeding bottles, and plates) for use by children and to measure the specific migration of these elements into beverages and foods. Total contaminant levels were determined using a handheld X-ray fluorescence analyzer. Specific migration tests were conducted using the simulant solutions acetic acid 3% (m/v) and water. Migration levels were determined by ICP-MS. Specific migration tests for Pb were also performed on commercially available samples (cola soft drink, orange juice, vinegar, and milk), with levels determined by graphite-furnace atomic absorption spectrometry (GF-AAS). A total of 674 utensils were analyzed in loco at major commercial centers in Greater São Paulo, of which 87 were purchased for containing Cd and Pb concentrations above permitted limits. Mean concentrations of the metals detected in the purchased utensils were 1110 ppm for Pb and 338 ppm for Cd. For specific migration assays, Pb levels were 187, 13, and 380 times above the permitted limit (0.01 mg.kg -1) for acetic acid, water, and orange juice, respectively. Cd levels were 50 and 2.4 times above the maximum permitted limit (0.005 mg.kg -1) for acetic acid and water, respectively. The districts where the utensils were purchased were grouped according to their social vulnerability index and compared using ANOVA. Pb levels were different between low and medium/high social vulnerability groups (p?=?0.006). The findings corroborate the initial hypothesis that these utensils constitute a major source of exposure to PTEs such as Cd and Pb, pointing to the need for stricter regulation and inspection by the Brazilian regulatory agencies.

     

Characterisation of airborne particles collected within and proximal to an opencast coalmine: South Wales,U.K

Airborne particulate matter has been collected from within,and proximal to, an opencast coal mine in south Wales. Thiswork forms the first part of a three year project to collectand characterise, then determine the possible toxicology ofairborne particles in the south Wales region. High-resolutionField Emission SEM has shown that the coal mine dusts consistlargely of an assemblage of mineral grains and vehicle exhaustparticles. SEM-EDX has shown that the mineralogical make-up ofthe PM10 is complex, heterogeneous, and constantly changing.These findings are supported by analytical TEM-EPXMA.However, patterns can be determined relating the mineralogicalcomposition of the airborne particles to collection locationsand mining activities within the opencast. At our studyopencast, Park Slip West, quartz, which has known healtheffects, never exceeded 30% of the total collection mass, andaverage levels were much less. Vehicle exhaust emissions wasthe largest source in terms of particle numbers. The mass ofairborne particulate matter within the pit averagedapproximately twice that of outside the pit: importantlyhowever, this higher mass was due to relatively large, andnon-respirable, mineral grains. This study demonstrates that the physicochemical andmineralogical characterisation of airborne particles frommining and quarrying is essential to quantify the respirablefraction, and to identify potentially hazardous componentswithin the PM10.     

The QUASIMEME laboratory performance study of polycyclic aromatic hydrocarbons (PAHs): assessment for the period 1996-1999. Quality Assurance of Information in Marine Environmental Monitoring in Europe

The results of the laboratory performance studies held during the period 1996-99 and involving the determination of PAHs in sediments and biota have been assessed. Overall 67% of the data submitted met the QUASIMEME criterion for satisfactory performance, although differences in performance were apparent between the two main analytical techniques employed: gas chromatography and high-performance liquid chromatography. Further development of PAH methodology utilising coupled gas chromatography mass spectrometry is encouraged as the most appropriate means of meeting the future requirements for the determination of a wider range of PAH analytes. More reference materials are also urgently required for use in marine monitoring programmes, and they need to be certified for a wider range of PAHs than hitherto, including both parent PAHs and their alkylated derivatives.     

Dealing with heterogeneous regression slopes in analysis of covariance: new methodology applied to environmental effects monitoring fish survey data

Analysis of covariance (ANCOVA) is a powerful statistical method which incorporates one or more covariates into the analysis to reduce error associated with measurement. ANCOVA (modeling response as a function of fish size) is frequently used to analyze environmental effects monitoring (EEM) fish survey data. In approximately 12% of fish survey data sets taken from cycles 1 to 3 of Environment Canada’s EEM database for pulp and paper mills, the standard assumption of parallel regression slopes is not met. For the first three cycles of the EEM program, these data sets were classified as indicating a mill effect, but for the most part were excluded from subsequent analyses aimed at quantifying the effect. We present two different methods for initially dealing with data sets that exhibit heterogeneous slopes so that they can be analyzed using the parallel slope model. The first method identifies data sets where heterogeneous slopes are forced by a few high-influence observations. The second approach identifies data sets where a model with heterogeneous slopes is statistically, but not practically, significant: with a high coefficient of determination for the parallel slope model. These new methodologies are applied to EEM pulp and paper data sets and about 55% of cases with heterogeneous slopes can be described by a parallel slope model. We also discuss a third method that can be used to describe mill effects when regression slopes remain heterogeneous even after applying the above two methods, enabling comparison with a critical effect size. These new methodologies could benefit the EEM program by enabling more data sets to be incorporated into meta-analyses and be used to make more equitable mill monitoring decisions in the future.     

Use of leaf litter breakdown and macroinvertebrates to evaluate gradient of recovery in an acid mine impacted stream remediated with an active alkaline doser

The spatial congruence of chemical and biological recovery along an 18-km acid mine impaired stream was examined to evaluate the efficacy of treatment with an alkaline doser. Two methods were used to evaluate biological recovery: the biological structure of the benthic macroinvertebrate community and several ecosystem processing measures (leaf litter breakdown, microbial respiration rates) along the gradient of improved water chemistry. We found that the doser successfully reduced the acidity and lowered dissolved metals (Al, Fe, and Mn), but downstream improvements were not linear. Water chemistry was more variable, and precipitated metals were elevated in a 3–5-km “mixing zone” immediately downstream of the doser, then stabilized into a “recovery zone” 10–18 km below the doser. Macroinvertebrate communities exhibited a longitudinal pattern of recovery, but it did not exactly match the water chemistry gradient Taxonomic richness (number of families) recovered about 6.5 km downstream of the doser, while total abundance and % EPT taxa recovery were incomplete except at the most downstream site, 18 km away. The functional measures of ecosystem processes (leaf litter breakdown, microbial respiration of conditioned leaves, and shredder biomass) closely matched the measures of community structure and also showed a more modest longitudinal trend of biological recovery than expected based on pH and alkalinity. The measures of microbial respiration had added diagnostic value and indicated that biological recovery downstream of the doser is limited by factors other than habitat and acidity/alkalinity, perhaps episodes of AMD and/or impaired energy/nutrient inputs. A better understanding of the factors that govern spatial and temporal variations in acid mine contaminants, especially episodic events, will improve our ability to predict biological recovery after remediation.     

Contemplating the Assessment of Great River Ecosystems

The science and practice of assessing the status and trends of ecological conditions in great rivers have not kept pace with perturbation wrought on these systems. Participants at a symposium sponsored by the U.S. Environmental Protection Agency (USEPA) and the Council of State Governments concluded that useful and efficient assessments of great river ecosystems require thoughtful alignment of sampling designs, spatial and temporal scales, indicators, management needs, and ecosystem characteristics. Site-specific physical, chemical, and biological data long accumulated by monitoring programs have value but fail to provide the integrated system-wide perspective required for adaptive management and the Clean Water Act. Use of existing data may be limited by methodological incompatibilities, access difficulties, and the exclusive applicability of data to specific habitats or sites. The transition from site-specific to system-wide assessments benefits from research being done by USEPA's Environmental Monitoring and Assessment Program (EMAP) and other programs that use probability surveys and biological indicators. Indicators of various taxa (in particular fish, algae, and benthic invertebrates) have been successfully developed for great rivers. However, optimizing the information these ecological indicators convey to managers and the public is the subject of ongoing research.     

The effect of different mitigation strategies on international financing of adaptation

Recent proposals at the UNFCCC meeting in Bali in December 2007 suggest that a 2% levy on the CDM could finance adaptation costs in developing regions. Other proposals include extending the scope of the levy to emissions trading. This study applies an Integrated Assessment Model to gain insight in the interactions between adaptation costs, residual damages and mitigation costs and to analyse the effectiveness of a 2% levy on both the CDM and emissions trading from developing countries. We show that adaptation is especially important in lower income regions where damages are higher. The revenues of a 2% levy strongly depend on both the climate mitigation target and the burden-sharing regime. A more stringent climate mitigation target results in more emissions trade and, in the longer run, less need for adaptation. Both factors increase the share of adaptation costs that can be funded. The burden-sharing regime strongly affects the revenues of a 2% levy as well: relatively more stringent targets for developed countries increase the revenues of a 2% levy. However, in the next two decades the share of adaptation that can be financed remains well below 20% in most cases. Additional funding mechanisms are therefore necessary to substantially finance adaptation costs in developing countries.