The ‘I see you’ prey–predator signal of Apis cerana is innate

An ‘I see you’ (ISY) prey–predator signal can co-evolve when such a signal benefits both prey and predator. The prey benefits if, by producing the signal, the predator is likely to break off an attack. The predator benefits if it is informed by the signal that the prey is aware of its presence and can break off what is likely to be an unsuccessful and potentially costly hunt. Because the signal and response co-evolve in two species, the behaviour underlying an ISY signal is expected to have a strong genetic component and cannot be entirely learned. An example of an ISY signal is the ‘shimmering’ behaviour performed by Asian hive bee workers in the presence of their predator Vespa velutina. To test the prediction that bee–hornet signalling is heritable, we let honey bee workers of two species emerge in an incubator so that they had never been exposed to V. velutina. In Apis cerana, the shimmering response developed 48 h post-emergence, was strong after 72 h and increased further over 2 weeks. In contrast, A. mellifera, which has evolved in the absence of Asian hornets, did not produce the shimmering signal. In control tests, A. cerana workers exposed to a non-threatening butterfly did not respond with the shimmering signal.     

Hepatitis A Virus: State of the Art

Hepatitis A is the most common among all hepatitis worldwide in spite of an efficient vaccine and improved hygiene. Shellfish-borne outbreaks are still of major concern causing hundreds of cases and huge economical losses in the present context of global food trade. Hepatitis A virus (HAV) is a unique picornavirus with many differences in its molecular biology including both its incapacity to induce the inhibition of the cellular protein synthesis and a highly biased and deoptimized codon usage with respect the cell. The final goal of this intriguing strategy seems to be the need for a fine-tuning control of the translation kinetics, particularly at the capsid coding region, and the underlying mechanism is the use of a right combination of common and rare codons to allow a regulated ribosome traffic rate thus ensuring the proper protein folding. Capsid folding is critical to warrant a high environmental stability for a virus transmitted through the fecal–oral route with long extracorporeal periods.     

Measuring the environmental performance of IPPC industry: II. Applying the Environmental Emissions Index to quantify environmental performance trends from routinely reported data

Reported emissions data were collated for 35 pharmaceutical-manufacturing installations and 18 power stations holding IPPC licences in Ireland. Consistent and essentially complete sectoral emissions time-series were generated, covering 2002–2006 for the pharmaceutical sector, and 2001–2006 for the electricity-generating sector. Applying the Environmental Emissions Index (EEI) to reported emissions indicated environmental performance improvements of 35 and 30%, respectively, for these two sectors. However, considerable uncertainty was attributed to reporting of heavy metals, NO_x and NMVOC emissions at the installation level, and overall NMVOC emissions from the pharmaceutical sector appeared to be considerably under-reported. The fixed average toxicity factor applied to NMVOC emissions in the EEI may deviate from potential temporal changes in the NMVOC compound mix. Overall, reporting uncertainties were found to have a greater impact on EEI outputs than assumptions made in the EEI model, and including an estimate of total sectoral NMVOC emissions reduced the pharmaceutical sector's environmental performance improvement to 24%. The EEI facilitates the comparison and visualisation of reported emissions, integrating them into environmental performance trends. It should optimise interpretation of abundant, detailed, and underutilised ‘bottom-up’ emissions data generated by IPPC installations. For Ireland's pharmaceutical sector, these data are considerably more comprehensive than EPER data.     

Using Bacteriophages in Recycled Water Exposure Assessment Studies

Bacteriophages MS-2 and PRD-1 were used as indicators of human viral behaviour in a series of turf-grass irrigation and washing machine experiments to provide information about the level of human exposure to potential residual enteric viruses in recycled water that can affect health. Results provided evidence to support a potential inadvertent exposure volume of 0.01 ml for recycled water used for machine clothes washing. Results for irrigation experiments showed that a one log reduction in bacteriophage numbers on turf-grass was not achieved under exposure to high solar conditions after 4 h. Experimentation showed that bacteriophages may be usefully employed in exposure assessment studies based on the ease of their enumeration and logistical and cost considerations. In terms of future studies, observed differences in individual bacteriophage characteristics provide a rationale for using multiple viral indicators. However, limitations on the utility of bacteriophage also need to be considered.     

Experimental evidence that keeping eggs dry is a mechanism for the antimicrobial effects of avian incubation

Avian incubation dramatically reduces the abundance and diversity of microbial assemblages on eggshells, and this effect has been hypothesized as an adaptive explanation for partial incubation, the bouts of incubation that some birds perform during the egg-laying period. However, the mechanisms for these antimicrobial effects are largely unknown. In this study, we hypothesized that microbial inhibition is partly achieved through removal of liquid water, which generally enhances microbial growth, from eggshells, and experimentally tested this hypothesis in two ways. First, we placed the first- and second-laid eggs of tree swallow (Tachycineta bicolor) clutches in unincubated holding nests with either ambient or increased water on eggshells. Second, we added water to eggshells in naturally partially incubated nests. We compared microbial growth on shells during a 5-day experimental period and found that, as predicted, both unincubated groups had higher microbial growth than naturally partially incubated controls, and that only in the absence of incubation did wetted eggs have higher microbial growth than unwetted eggs. Thus, we have shown that water increases microbial growth on eggshells and that incubation nullifies these effects, suggesting that removal of water from egg surfaces is one proximate mechanism for the antimicrobial effects of incubation.     

Imperfect chemical female mimicry in males of the ant Cardiocondyla obscurior

Winged and wingless males coexist in the ant Cardiocondyla obscurior. Wingless (“ergatoid”) males never leave their maternal colony and fight remorselessly among each other for the access to emerging females. The peaceful winged males disperse after about 10 days, but beforehand also mate in the nest. In the first 5 days of their life, winged males perform a chemical female mimicry that protects them against attack and even makes them sexually attractive to ergatoid males. When older, the chemical profile of winged males no longer matches that of virgin females; nevertheless, they are still tolerated, which so far has been puzzling. Contrasting this general pattern, we have identified a single aberrant colony in which all winged males were attacked and killed by the ergatoid males. A comparative analysis of the morphology and chemical profile of these untypical attacked winged males and the tolerated males from several normal colonies revealed that normal old males are still performing some chemical mimicry to the virgin queens, though less perfect than in their young ages. The anomalous attacked winged males, on the other hand, had a very different odour to the females. Our study thus exemplifies that the analysis of rare malfunctioning can add valuable insight on functioning under normal conditions and allows the conclusion that older winged males from normal colonies of the ant C. obscurior are guarded through an imperfect chemical female mimicry, still close enough to protect against attacks by the wingless fighters yet dissimilar enough not to elicit their sexual interest.     

Male-biased predation of western green lizards by Eurasian kestrels

Selective predation can be an important force driving the evolution of organisms. In particular, sex-biased predation is expected to have implications for sexual selection, sex allocation and population dynamics. In this study, we analysed sex differences in the predation of the western green lizard (Lacerta bilineata) by the Eurasian kestrel (Falco tinnunculus) during the reproductive season. In addition, we investigated whether the rate of predation differed during the 8-year study period and among the three habitats studied. We collected lizard remains from nest boxes of kestrels. Freshly killed lizards were sexed by visual inspection, whilst the sex of head remains was assigned by analysing the cephalic scale morphology using geometric morphometrics. Our results show that the risk of being predated by a kestrel in our population was overall about 3.55 times higher for males than for females. To our knowledge this is the first study showing a male-biased predation in a lizard species. The selective predation of males was consistent between years over the 8-year study period (1999-2006) and also consistent between the three types of kestrel hunting habitat. Overall predation rates on lizards differed between habitats, depending on the year. We propose that the observed sex-biased predation is mainly due to sex differences in lizard behaviour.     

Primary prevention for worker health and safety: cleaner production and toxics use reduction in Massachusetts

Worker health and safety and environmental protection are not always considered simultaneously when attempting to reduce or eliminate hazardous materials from our environment. Cleaner Production-Pollution Prevention (CPPP), as primary prevention, has the ability to shift worker health and safety strategies from control to prevention, where exposure prevention precedes exposure control. This paper evaluates the effect of Cleaner Production-Pollution Prevention in the form of toxics use reduction (TUR) on worker health and safety at three printed wire board facilities covered under the Massachusetts Toxics Use Reduction Act. In-depth case study analysis, including an assessment of each facility’s health and safety status, explores the root causes of the worker health and safety changes attributable to the TUR interventions. By exploring the relationship between worker health and safety and environmental protection within the corporate structure; we can identify the factors driving companies to reduce toxics both inside and outside of their plants, as a single concern.While traditionally there have been divergent paths of practice for worker health and safety and environmental protection, the two are closely connected. It is important, however, to consider the implications of risk transfer/shifting between the general and work environment. In order to avoid this risk shifting, worker health and safety perspectives and goals must be more clearly incorporated into the Cleaner Production-Pollution Prevention/TUR management system. This study opens a dialog around the effects of environmental intervention programs on worker health and safety. We realize now that while CPPP/TUR reduces exposure to toxic substances in the general environment, it also offers unique opportunities to reaffirm primary prevention principles in the work environment.     

Effects of LATCH versus Available Seatbelt Installation of Rear Facing Child Restraint Systems on Head Injury Criteria for 6 Month Old Infants in Rear End Collisions

Objective: The Lower Anchor and Tethers for CHildren (LATCH) system was introduced in vehicles made after September 1, 2002 and intended to make installation of rear and forward-facing child safety seats easier. Due to the lack of rear impact testing of RFCRS required per the Federal Motor Vehicle Safety Standards (FMVSS), the purpose of this study was to explore the effects, if any, of installation method of RFCRS on the performance of commonly purchased makes and models of RFCRS. Specifically, we hypothesize that in a 48 km/h (29.8 MPH) rear-end collision, installation of RFCRS using the LATCH system will result in higher Head Injury Criteria (HIC) values when compared to using the available lap/shoulder seatbelt (Emergency Locking Retractor - ELR or Automatic Locking Retractor - ALR).

Methods: The test matrix included 36 rear impact sled tests conducted using 3 installation methods on 3 models of RFCRS: the Graco SnugRide® with and without the base, the Britax Chaperone with base-mounted anti-rebound bar, and the Evenflo Tribute®, a model of convertible rearward/forward facing restraint system used in the rearward facing mode. The seats were installed using the LATCH system, ELR lap/shoulder belts, or ALR lap/shoulder belts in seating positions 4 and 6 on a vehicle buck mounted to the sled test base. The infant seat and 6 month old CRABI anthropometric test device (ATD) installation methods were in accordance with standards set forth in the National Highway Traffic Safety Administration's (NHTSA) FMVSS No. 213, Child Restraint Systems. All tests were conducted on pneumatic controlled acceleration sled (HYGE, Inc., PA, USA) at 48 km/h.

Results: Installation of infant seat type RFCRS using the LATCH system resulted in higher HIC₁₅ values when compared to using the available lap/shoulder seatbelt (ELR or ALR). The mean HIC₁₅ values were most severe when infant seat type RFCRS were installed using LATCH (Graco SnugRide® HIC₁₅ = 394 and Britax Chaperone HIC₁₅ = 133) compared to using either ELR lap/shoulder belts (Graco SnugRide® HIC₁₅ = 218 and Britax Chaperone HIC₁₅ = 65) or ALR lap/shoulder belts (Graco SnugRide® HIC₁₅ = 194 and Britax Chaperone HIC₁₅ = 78). The installation method did not result in a statistically significant difference in HIC for the convertible type RFCRS (Evenflo Tribute®). In many of the tests, the ATD's head struck the seatback in which the RFCRS was installed. These head strikes resulted in the higher HIC₁₅ scores recorded throughout the testing.

Conclusions: The results of this study suggest that LATCH does not offer equal protection to lap/shoulder belts from head injuries in rear impacts when used with infant seat type RFCRS.