Estimation of β-cyfluthrin and imidacloprid in okra fruits and soil by chromatography techniques

Dissipation of β-cyfluthrin and imidacloprid in okra was studied following three applications of a combination formulation of Solomon 300 OD (β-cyfluthrin 9 % + imidacloprid 21 %) @ 60 and 120 g a.i. ha^?1 at 7 days interval. Residues of β-cyfluthrin and imidacloprid in okra were estimated by gas liquid chromatography (GLC) and high performance liquid chromatography (HPLC), respectively. Residues of β-cyfluthrin were confirmed by gas chromatograph–mass spectrometry (GC-MS) and that of imidacloprid by high performance thin layer chromatography (HPTLC). Half-life periods for β-cyfluthrin were found to be 0.91 and 0.68 days whereas for imidacloprid these values were observed to be 0.85 and 0.96 days at single and double the application rates, respectively. Residues of β-cyfluthrin dissipated below its limit of quantification (LOQ) of 0.01 mg kg^?1 after 3 and 5 days at single and double the application dosage, respectively. Similarly, residues of imidacloprid took 5 and 7 days to reach LOQ of 0.01 mg kg^?1, at single and double dosages respectively. Soil samples collected after 15 days of the last application did not show the presence of β-cyfluthrin and imidacloprid at their detection limit of 0.01 mg kg^?1.     

Integrated Bacillus sp. immobilized cell reactor and Synechocystis sp. algal reactor for the treatment of tannery wastewater

The wastewater discharged from leather industries lack biodegradability due to the presence of xenobiotic compounds. The primary clarification and aerobic treatment in Bacillus sp. immobilized Chemo Autotrophic Activated Carbon Oxidation (CAACO) reactor removed considerable amount of pollution parameters. The residual untreated organics in the wastewater was further treated in algal batch reactor inoculated with Synechocystis sp. Sodium nitrate, K₂HPO₄, MgSO₄.7H₂O, NH₄Cl, CaCl₂·2H₂O, FeCl₃ (anhydrous), and thiamine hydrochloride, rice husk based activated carbon (RHAC), immobilization of Bacillus sp. in mesoporous activated carbon, sand filter of dimensions diameter, 6 cm and height, 30 cm; and the CAACO reactor of dimensions diameter, 5.5 cm and height, 30 cm with total volume 720 ml, and working volume of 356 ml. In the present investigation, the CAACO treated tannery wastewater was applied to Synechocystis sp. inoculated algal batch reactor of hydraulic residence time 24 h. The BOD₅, COD, and TOC of treated wastewater from algal batch reactor were 20?±?7, 167?±?29, and 78?±?16 mg/l respectively. The integrated CAACO system and Algal batch reactor was operated for 30 days and they accomplished a cumulative removal of BOD₅,COD, TOC, VFA and sulphide as 98 %, 95 %, 93 %, 86 %, and 100 %, respectively. The biokinetic constants for the growth of algae in the batch reactor were specific growth rate, 0.095(day^?1) and yield coefficient, 3.15 mg of algal biomass/mg of COD destructed. The degradation of xenobiotic compounds in the algal batch reactor was confirmed through HPLC and FT-IR techniques. The integrated CAACO–Algal reactor system established a credible reduction in pollution parameters in the tannery wastewater. The removal mechanism is mainly due to co-metabolism between algae and bacterial species and the organics were completely metabolized rather than by adsorption.     

High reduction of 4-nitrophenol using reduced graphene oxide/Ag synthesized with tyrosine

There is a demand for the development of environmental friendly methods for the synthesis of graphene composites. Reduced graphene oxide/silver (RGO/Ag) nanocomposites are very good catalysts. Here, we propose a simple, green method for the synthesis of RGO/Ag nanocomposite using the amino acid tyrosine as bioreductant and stabilizing agent. RGO/Ag nanocomposite was characterized by using various analytical techniques and studied for its catalytic degradation of 4-nitrophenol. Results of attenuated total reflectance Fourier transform infrared spectroscopy and Zeta potential at ?55 mV reveal the surface capping of tyrosine onto the reduced graphene oxide nanosheets. RGO/Ag nanocomposites show excellent catalytic reduction of 4-nitrophenol with NaBH₄, when compared to actual individual silver nanoparticles.     

Volatilization of mercury from natural water by a broad-spectrum Hg-resistantBacillus pasteurii strain DR2

Summary A broad-spectrum mercury-resistant bacterial strain was isolated from contaminated water and was identified as Bacillus pasteurii strain DR2. It could volatilize Hg-compounds including organomercurials from its growth media. It utilized several aromatic compounds as a sole source of carbon. The bacterial strain eliminated HgCl₂ from sterile river water and the presence of benzene, toluene, naphthalene and nitrobenzene at 1 mM concentration in the system increased the rate of mercury volatilization, the volatilization rate being highest with benzene. When 1.7×10⁷ cells of this bacterial strain were added per ml of non-sterile water the bacterial strain volatilized more than 90 percent of mercury from mercuric chloride and organo-mercurials like PMA, thiomersol and methoxy ethyl mercuric chloride (MEMC). In the absence of this bacterial strain the volatilization of PMA and MEMC due to the presence of other Hg-resistant organisms in nonsterile polluted water ranged between 20–25 percent and of HgCl₂ was about 40 percent. However, in the presence of B. pasteurii DR2 volatilization of these Hg-compounds from non-sterile water increased by 20–40 percent. In the presence of 1 mM benzene the rate of mercury volatilization was even higher. In all the cases the rate of volatilization was higher in the first seven days than in the next seven days.Professor A. Mandal, MSc, PhD is Head of the Department of Biochemistry at the University College of Science, 35 Ballygunge Circular Road, Calcutta 700019, to whom correspondence should be addressed. His co-workers are Dr K. Pahan, Postdoctoral Associate, Department of Cell Biology and Paediatrics, Medical University of South Carolina, USA; Dr J. Chaudhuri, Senior Lecturer, Department of Molecular Biology, BKC College, Calcutta, India; Dr D.K. Ghosh, Postdoctoral Associate, Department of Biochemistry, University College of Science, Calcutta, India; Dr R. Gachhui, Postdoctoral Associate, The Cleveland Clinic Foundation, Cleveland, USA; and Dr S. Ray, Postdoctoral Associate, The Johns Hopkins University School of Hygiene and Public Health, Department of Biochemistry, Baltimore, USA.     

Greenhouse gas emissions from rice based cropping systems: Economic and technologic challenges and opportunities

Recent market slump in rice, less rainfall during monsoon, high temperature and scarcity of water during dry season leads to lower grain yield and less profit from rice cultivation in India. Farmers’ grow upland crops like chickpea (Cicer arietinum), greengram (Vigna radiate), mustard (Brassica nigra), corn (Zea maize), pigeonpea (Cajanus cajan), potato (Solanum tuberosum), sunflower (Helianthus annuus) etc. along with rice (Oryza sativa) during the dry season. However, knowledge of greenhouse gas (GHG) emission from these rice based cropping systems is very limited. In the present study four rice based cropping systems was studied along with rice-rice rotation system as control in respect of GHG emission, yield potential and economic feasibility. Conventional plantation and fertilizer application methodology was followed for each crop. Methane (CH₄) and nitrous oxide (N₂O) flux from field plots were studied with conventional closed chamber method using gas chromatograph. CH₄ flux was recorded highest from rice-rice rotation plots (304.25 kg ha⁻¹). N₂O flux was recorded 1.02 kg ha⁻¹ from rice-rice rotation system during wet season. However, during wet season, higher N₂O flux (1.93 kg ha⁻¹) was recorded from rice-potato-sesame rotation plots. Annual N₂O flux was also recorded significantly low (3.42 kg ha⁻¹) from rice-rice rotation plots and high (6.19 kg ha⁻¹) from rice-chickpea-greengram rotation plots. Significantly lower annual grain yield was recorded from rice-rice rotation plots (9.25 Mg ha⁻¹) whereas it was 18.84 Mg rice eq ha⁻¹ from rice-potato-sesame rotation system. The global warming potential (GWP) of rice-rice rotation system was recorded significantly high (8.62 Mg CO₂ ha⁻¹) compare to plots with different rice based cropping systems. Computing all C-emission from cradle-to-grave, highest total C-cost was recorded from the rice-rice rotation system ($62.00 ha⁻¹). We have made an attempt to calculate the C-credit of different rice based cropping systems by considering the difference of C-cost with control. The study suggests that the rice-potato-sesame is most sustainable among different cropping system studied in terms of economic profit ($62.00 ha⁻¹). We have made an attempt to calculate the C-credit of different rice based cropping systems by considering the difference of C-cost with control. The study suggests that the rice-potato-sesame is most sustainable among different cropping system studied in terms of economic profit (1248.21 ha⁻¹) and C-credit ($38.60 ha⁻¹). The result of the study may be limited to the study region; however, the study has potential use in respect to the development of agriculture practice for adaptation to climate change.     

Kinetic and isotherm error optimization studies for adsorption of atrazine and imidacloprid on bark of Eucalyptus tereticornis L.

The aim of this study was to establish the bark of Eucalyptus tereticornis L. (EB) as a low cost bio-adsorbent for the removal of imidacloprid and atrazine from aqueous medium. The pseudo-first-order (PFO), pseudo-second-order (PSO), Elovich and intra-particle diffusion (IPD) models were used to describe the kinetic data and rate constants were evaluated. Adsorption data was analysed using ten 2-, 3- and 4-parameter models viz. Freundlich, Jovanovic, Langmuir, Temkin, Koble–Corrigan, Redlich–Peterson, Sips, Toth, Radke–Prausnitz, and Fritz-Schluender isotherms. Six error functions were used to compute the best fit single component isotherm parameters by nonlinear regression analysis. The results showed that the sorption of atrazine was better explained by PSO model, whereas the sorption of imidacloprid followed the PFO kinetic model. Isotherm model optimization analysis suggested that the Freundlich along with Koble–Corrigan, Toth and Fritz-Schluender were the best models to predict atrazine and imidacloprid adsorption onto EB. Error analysis suggested that minimization of chi-square (χ²) error function provided the best determination of optimum parameter sets for all the isotherms.     

Loss of crop yields in India due to surface ozone: an estimation based on a network of observations

Surface ozone is mainly produced by photochemical reactions involving various anthropogenic pollutants, whose emissions are increasing rapidly in India due to fast-growing anthropogenic activities. This study estimates the losses of wheat and rice crop yields using surface ozone observations from a group of 17 sites, for the first time, covering different parts of India. We used the mean ozone for 7 h during the day (M7) and accumulated ozone over a threshold of 40 ppbv (AOT40) metrics for the calculation of crop losses for the northern, eastern, western and southern regions of India. Our estimates show the highest annual loss of wheat (about 9 million ton) in the northern India, one of the most polluted regions in India, and that of rice (about 2.6 million ton) in the eastern region. The total all India annual loss of 4.0–14.2 million ton (4.2–15.0%) for wheat and 0.3–6.7 million ton (0.3–6.3%) for rice are estimated. The results show lower crop loss for rice than that of wheat mainly due to lower surface ozone levels during the cropping season after the Indian summer monsoon. These estimates based on a network of observation sites show lower losses than earlier estimates based on limited observations and much lower losses compared to global model estimates. However, these losses are slightly higher compared to a regional model estimate. Further, the results show large differences in the loss rates of both the two crops using the M7 and AOT40 metrics. This study also confirms that AOT40 cannot be fit with a linear relation over the Indian region and suggests for the need of new metrics that are based on factors suitable for this region.     

A framework for assessing vulnerability of inland fisheries to impacts of climate variability in India

In recent years climate variability has threatened the sustainability of inland fisheries and dependent fishers in India. Systematic methodology to assess the vulnerability of the fisheries sector to climate variability is currently not available. Towards this end, the present work deals with the assessment of inland fisheries vulnerability to climate variations in 13 districts of West Bengal state in India. For this purpose, a composite vulnerability index (0.0–1.0) has been developed on the basis of functional relationships amongst sensitivity, exposure and adaptive capacity using 19 indicators related to inland fisheries. The data obtained reflected different spatial combinations of climate exposure, sensitivity and adaptive capacity among the districts. Five districts were highly vulnerable which was attributable to low adaptive capacity of the fishers which played an important role in altering the spatial pattern of vulnerability among the districts. Thus our research will provided an important basis for policy makers to develop appropriate adaptation strategies to minimize the risk of fisheries sector to climate variability.     

Contribution of individual components of a job cycle on overall severity of whole-body vibration exposure: a study in Indian mines

Drivers of earth-moving machines are exposed to whole-body vibration (WBV). In mining operations there can be a combination of relatively high magnitudes of vibration and long exposure times. Effective risk mitigation requires understanding of the main aspects of a task that pose a hazard to health. There are very few published studies of WBV exposure from India. This paper reports on a study that considered the contribution of the component phases of dumper operations, on the overall vibration exposure of the drivers. It shows that vibration magnitudes are relatively high, and that haulage tasks are the main contributor to the exposure. It is recommended that driver speed, haul road surfaces and vehicle maintenance/selection are optimized to ensure minimization of vibration. If this is not sufficient, operation times might need to be reduced in order to ensure that the health guidance caution zone from Standard No. ISO 2631-1:1997 is not exceeded.