Triploidy: Pregnancy complications and clinical findings in seven cases

Seven cases of triploidy were encountered by the Prenatal Diagnosis Program at Dartmouth–Hitchcock Medical Center over an 8-year period through associated pregnancy complications. We describe the characteristic findings that facilitate prenatal diagnosis and management. Our experience includes fetuses with major central nervous system abnormalities (spina bifida aperta, holoprosencephaly) and anterior abdominal wall defects, which are detectable with routine prenatal diagnostic screening examinations (ultrasound and AFP). In addition, we stress the importance of recognizing obstetric complications and associated cystic placental changes, which are quite common among triploid conceptuses. Molar changes associated with triploidy have a more benign prognosis than that associated with diploid moles. Such molar changes may relate to the presence of a diploid paternal chromosome complement. The usefulness of cytofluorometric DNA determinations in helping to confirm a clinical suspicion of triploidy is emphasized. These cases are presented in an effort to facilitate prenatal recognition and management of this common cytogenetic condition and prevent unnecessary Caesarean section deliveries.     

Growth and development of nauplii and copepodites of the estuarine copepod Acartia tonsa from southern Europe (Ria de Aveiro, Portugal) under saturating food conditions

A temperature-dependent growth model is presented for nauplii and copepodites of the estuarine calanoid copepod Acartia tonsa from southern Europe (Portugal). Development was followed from egg to adult in the laboratory at four temperatures (10, 15, 18 and 22°C) and under saturating food conditions (>1,000 μg C l⁻¹). Development times versus incubation temperature were fitted to a Belehradek’s function, showing that development times decreased with increasing incubation temperature: at 10°C, A. tonsa need 40.3 days to reach adult stage, decreasing to 8.9 days when reared at 22°C. ANCOVA (homogeneity of slopes) showed that temperature (P<0.001) and growth phase (P<0.01) had a significant effect on the growth rate. Over the range of temperatures tested in this study, highest weight-specific growth rates were found during naupliar development (NI–NVI) and varied from 0.185 day⁻¹ (10°C) to 0.880 day⁻¹ (22°C) with a Q ₁₀ equal to 3.66. During copepodite growth (CI–CV), the weight-specific growth rates ranged from 0.125 day⁻¹ (10°C) to 0.488 day⁻¹ (22°C) with a Q ₁₀ equal to 3.12. The weight-specific growth rates (g) followed temperature (T) by a linear relationship and described as ln g=−2.962+0.130 T (r ²=0.99, P<0.001) for naupliar stages and ln g=−3.134+0.114T (r ²=0.97, P<0.001) for copepodite stages. By comparing in situ growth rates (juvenile growth and fecundity) for A. tonsa taken from the literature with the temperature-dependent growth model defined here we suggest that the adult females of A. tonsa are more frequently food limited than juveniles.     

Landscape evaluation in conservation: molecular sampling and habitat modeling for the Iberian lynx.

Conservation of endangered species requires comprehensive understanding of their distribution and habitat requirements, in order to implement better management strategies. Unfortunately, this understanding is often difficult to gather at the short term required by rapidly declining populations of many rare vertebrates. We present a spatial habitat modeling approach that integrates a molecular technique for species detection with landscape information to assess habitat requirements of a critically endangered mammalian carnivore, the Iberian lynx (Lynx pardinus), in a poorly known population in Spain. We formulated a set of model hypotheses for habitat selection at the spatial scale of home ranges, based on previous information on lynx requirements of space, vegetation, and prey. To obtain the required data for model selection, we designed a sampling protocol based on surveys of feces and their molecular analysis for species identification. After comparing candidate models, we selected a parsimonious one that allowed (1) reliable assessment of lynx habitat requirements at the scale of home ranges, (2) prediction of lynx distribution and potential population size, and (3) identification of landscape management priorities for habitat conservation. This model predicted that the species was more likely to occur in landscapes with a higher percentage of rocky areas and higher cover of bushes typical of mature mediterranean shrubland mosaics. Its accuracy for discriminating lynx presence was approximately 85%, indicating high predictive performance. Mapping model predictions showed that only 16% of the studied areas constitute potential habitat for lynx, even though the region is dominated by large extents of well-preserved native vegetation with low human interference. Habitat was mostly clumped in two nearby patches connected by vegetation adequate for lynx dispersal and had a capacity for 28-62 potential breeding territories. The lynx population in Sierra Morena is probably the largest persisting today, but it is still critically small for optimism about its long-term persistence. Model results suggest habitat conservation and restoration actions needed for preserving the species, including reconciliation of hunting management with preservation of mature shrubland over large areas (particularly in rocky landscapes). The approach presented here can be applied to many other species for which the ecological information needed to develop sound habitat conservation strategies is lacking.     

The small-scale water provider in Paraguay: bringing private sector efficiency to water resource use and the provision of drinking water to the poor

This article provides a case study of small-scale private sector provision of water supply in Paraguay, where the Government has sought sector policy reforms that would encourage private investment in drinking water supply. Ironically, while the Government has focused almost entirely on garnering the interest of large private international water companies, much smaller local firms have already made significant investments in drinking water services for the poor, all without any participation or encouragement from the Government. Outside Paraguay's two major cities, Asunción and Ciudad del Este, large numbers of aguateros currently provide piped potable water to lower-income people. Though the aguateros have little legal footing — they are in many respects informal and unregulated —they have constructed as much as one third of all the new drinking water connections in these two cities over the past 20 years. The small-scale water systems in Paraguay offer a model of financial, economic, and water-use efficiency. This article asserts that an abundance of groundwater resources, cheap access to electricity for pumping, and a spirit of informal investment, among other variables, has spawned widespread use of this approach. This article documents and analyzes the features of these independent small-scale water providers in Paraguay and the efficiency they bring to the use of water resources in meeting drinking water demands among the poor. It also cautions against policies that may trample on such entrepreneurial spirit in the name of State-managed privatization.     

Developing sustainable agricultural systems for small farmers in Latin America

The great majority of farmers in Latin America are peasants who still farm small plots of land, usually in marginal environments utilizing traditional and subsistence methods. The contribution of the 16 million peasant units to regional food security is however substantial. Research has shown that peasant systems, which mostly rely on local resources and complex cropping patterns, are reasonably productive despite their small land endowments and low use of external inputs. Moreover analysis of NGO-led agroecological initiatives show that traditional crop and animal systems can be adapted to increase productivity by biologically re-structuring peasant farms which in turn leads to optimization of key agroecosystem processes (nutrient cycling, organic matter accumulation, biological pest regulation, etc.) and efficient use of labour and local resources. Examples of such grassroots projects are herein described to show that agroecological approaches can offer opportunities to substantially increase food production while preserving the natural resource base and empowering rural communities.     

Large-scale shrimp farming in coastal wetlands of Venezuela,South America: Causes and consequences of land-use conflicts

In Venezuela, large-scale shrimp farming began in the 1980s. By 1987, the Ministry of Environment and Natural Resources (MARNR) had received 14 proposals for approval. A developer illegally started the construction of ponds at the Píritu Lagoon in the State of Anzoátegui before the authorization process was completed. This action triggered a land-use conflict. This study identifies the causes for public protest and determines the consequences of this conflict for land-use management. The results show that public protest was based on the impacts of the partial construction of ponds. These impacts were related to direct removal of wetlands, interruption of natural patterns of surface flows, and alteration of feeding grounds of some bird species with migratory status. Consequences were identified in relation to the role that nongovernmental organizations (NGOs) play in land-use conflicts and the actions that MARNR could take in the future to prevent and solve similar situations.     

Land-cover changes and recent hydrological evolution in the Duero Basin (Spain)

Land-use and land-cover changes have attracted substantial scientific interest in recent years because of their marked influence on hydrological cycles. In developed countries of the Mediterranean basin, the generalized revegetation and forest growth in mountainous areas that occurred during the last five decades are negatively affecting the evolution of water resources in headwaters. In this study, changes in land cover in the Duero River Basin (northern Spain) during the last 50 years were analyzed and their role in hydrological evolution was estimated. For this purpose, step-wise linear regressions were developed to estimate the evolution of runoff as a function of climate (precipitation and temperatures). The results show a significant expansion of forest cover in the headwaters, although it has been more extensive in the mountains to the north of the basin than to the south. River discharges in the headwaters underwent a generalized decline during the study period (1961–2006), but precipitation over the same period did not show an appreciable trend. In the absence of noticeable trends in removal of water for human consumption, our results indicate that revegetation is contributing to the observed hydrological decline. Our hypothesis is confirmed because of the greater divergence in the evolution of precipitation and runoff in the northern headwaters (more forest growth) than in the south headwaters (less forest growth). Results suggest that further increases in forest area will enhance hydrological decline and highlight the importance of integrating land-cover information in water availability assessments in a region where water is a strategic resource.