Ozone-induced changes in CO2 assimilation, O2 evolution and chlorophyll a fluorescence transients in barley

Spring barley (Hordeum vulgare cv. Klaxon) plants, 9 days old, were exposed to 0.05, 0.10 or 0.15 microl litre(-1) ozone (O3) for 12 days. Fumigation was administered for 7 h between 9.00 h and 16.00 h each day. Using conventional IRGA equipment, the carbon dioxide exchange rate (CER) was shown to decrease with increasing concentration of O3 during the exposure period, falling to 60% of the control value at the highest O3 concentration. Transpiration rates and stomatal conductance showed similar trends. Light saturation curves, obtained using a leaf disc oxygen electrode, demonstrated that O3-treated leaves had lower apparent quantum yields (QY) and generally lower rates of O2 evolution at saturating light and CO2 levels. Oscillations in chlorophyll a fluorescence, normally observed in control plants, could not be detected after O3 treatment and could only be restored to some extent by feeding the phosphate sequestering agent D-mannose to the leaves.     

Oxidant air pollution effects on plants of Joshua Tree National Monument

Joshua Tree National Monument (JOTR) is located about 100 km east of the Los Angeles Basin, site of the heaviest concentration of photochemical oxidant (O(3)) air pollution in the US. This investigation was conducted to measure O(3) concentrations in JOTR and to determine the effects of O(3) on vegetation in the park. Potentially phytotoxic concentrations of O(3) were recorded in JOTR in 1984 and 1985, but peak concentration occurred at night, when most plant species would be less sensitive to O(3). No O(3) effects were observed on permanent vegetation observation plots in JOTR in 1984 or 1985. Controlled exposures of native summer annual and woody perennial species to O(3) showed that most did not develop visible O(3) injury symptoms except at concentrations higher than those expected in the park. However, Rhus trilobata Nutt. was injured at 0.10 ppm O(3), 4 h per day for 4 days. This species would be a useful bioindicator to assess the effects of O(3) on native desert plants.     

Selecting ozone exposure statistics for determining crop yield loss from air pollutants

Numerous ozone exposure statistics were calculated using hourly ozone data from crop yield loss experiments previously conducted for alfalfa, fresh market and processing tomatoes, cotton, and dry beans in an ambient ozone gradient near Los Angeles, California. Exposure statistics examined included peak (maximum daily hourly) and mean concentrations above specific threshold levels, and concentrations during specific time periods of the day. Peak and mean statistics weighted for ozone concentration and time period statistics weighted for hour of the day were also determined. Polynomial regression analysis was used to relate each of 163 ozone statistics to crop yield. Performance of the various statistics was rated by comparing residual mean square (RMS) values. The analyses demonstrated that no single statistic was best for all crop species. Ozone statistics with a threshold level performed well for most crops, but optimum threshold level was dependent upon crop species and varied with the particular statistics calculated. The data indicated that daily hours of exposure above a critical high-concentration threshold related well to crop yield for alfalfa, market tomatoes, and dry beans. The best statistic for cotton yield was an average of all daily peak ozone concentrations. Several different types of ozone statistics performed similarly for processing tomatoes. These analyses suggest that several ozone summary statistics should be examined in assessing the relationship of ambient ozone exposure to crop yield. Where no clear statistical preference is indicated among several statistics, those most biologically relevant should be selected.     

Biochemical response of Norway spruce (Picea abies (L.) Karst.) towards 14-month exposure to ozone and acid mist: effects on amino acid, glutathione and polyamine titers

Two clones of Norway spruce were exposed to elevated ozone levels (100 microg m(-3) with episodes of 130-360 microg m(-3)) in combination with acidic mist (pH 3.0) for two vegetation periods. The plants did not exhibit any visible injury, but levels of several amino acids and polyamines were altered in comparison with control plants (50 microg m(-3) ozone, mist of pH 5.6), the changes being pronounced in clone 14. Total free amino acids as well as methionine titers were increased in clone 14. Asparagine was significantly increased in clone 11 and less so in clone 14. Arginine, which comprised more than 50% of the free amino acids in spruce needles, was not changed by the exposure regime applied. Reduced glutathione was significantly increased in all clones/soil/needle age combinations (average increase 50%). Free soluble putrescine was enhanced by 50-200% in clone 14, but remained unchanged in clone 11. Conjugated putrescine was significantly, and conjugated spermidime was slightly, increased in both clones, whereas other polyamines did not responde to the treatment.     

Stage dependent susceptibility to lead in Bufo arenarum embryos

The stage dependent susceptibility to lead in amphibian development was studied by exposing Bufo arenarum embryos during neurulae, neuromuscular activity and gill circulation stages for twenty hours to 1 ppm Pb(2+). Survival, malformations and behavioral disorders were evaluated. The embryonic susceptibility to lead was markedly stage dependent. The survival at the neuromuscular activity stage was approximately half that of the other two periods; concerning malformations, the gill circulation stage was the least sensitive. The observed malformations consisted of failed closure of neural tube, hydropsy, small and cylindrical tails, reduced body size and incurvations in the body axis. Some alterations occurred in all experimental groups and therefore were considered non-dependent on the period of treatment. In all experimental embryos, neurological disorders such as trembles and loss of equilibrium were observed.     

Nutrient accumulation in trees and soil following irrigation with municipal effluent in Australia

Irrigation of tree crops is being evaluated as a method of land disposal of municipal effluent in Australia. A study was carried out from 1980-84 in which seven tree species were sprinkler-irrigated with effluent at an annual rate of 1191-1752 mm. Effective weed control and frequent irrigation resulted in good survival of all species (range 83-100%) at 12 months. Total productivity was estimated at age 4 years by measuring biomass of each species inclusive of litter and roots to a soil depth of 80 cm. Biomass production of the high-yielding species, flooded gum (Eucalyptus grandis) and Sydney blue gum (E. saligna), was around 10 kg m(-2). Percentage leaf mass of these species was small (8-9%) compared with 25% and 29% for the relatively slow-growing river she-oak (Casuarina cunninghamiana) and radiata pine (Pinus radiata). Accumulation of nutrients in the total biomass differed significantly between species and ranged from 34-54 g m(-2) for nitrogen, 4.0-10.4 g m(-2) for phosphorus, 2.1-12.2 g m(-2) for sodium, 22-34 g m(-2) for potassium, 12-61 g m(-2) for calcium and 4.7-9.3 g m(-2) for magnesium. River she-oak and river red gum (E. camaldulensis), because of their relatively large crown and litter masses, accumulated more nitrogen, phosphorus, potassium and calcium than flooded gum or Sydney blue gum. Chemical properties of soils (0-150 cm) were measured in 1980 and again in 1984. Irrigation significantly increased pH (by around 1 unit), throughout the profile. Concentrations of total phosphorus, and exchangeable sodium, calcium and magnesium were increased in the upper profile. Overall, soil chemical properties were not adversely affected by effluent irrigation over the 4-year period, though there was a trend towards more sodic conditions in the soil profile. Nutrient accumulation in soil occurred mainly in the 0-35 cm depth, coinciding with the main root zone of the trees. Renovation of the effluent was therefore estimated as the amount of each nutrient accumulated in the biomass (averaged over the seven species) plus soil (0-35 cm), expressed as a percentage of amount applied in irrigation over the 4 years; that is, nitrogen, 29%; phosphorus, 78%; sodium, 15%; potassium, 26%; calcium, 98% and magnesium, 54%.     

Effect of nitrogenous air pollutants on changes in protein spectra with the onset of winter in the leaves and shoots of the bilberry (Vaccinium myrtillus L.)

Electrophoretic profiles of 0.1 m Tris-HCl (pH 9.0)-soluble proteins in the leaves and stems of the bilberry (Vaccinium myrtillus L.) were investigated in the latter part of the growing season (July-October), in a highly-polluted industrial area with serious forest damage (main emissions NO(2), SO(2), NH(3), urea and fertilizer dust) in Oulu, northern Finland, and in an unpolluted control forest with the same climatic and edaphic conditions. About ten protein components in the leaves and seven in the stems increased in concentration in SDS-PAGE towards the autumn, while one to four other components decreased in concentration. The pollutants in the industrial area caused a considerable decrease in the concentrations of soluble proteins, particularly in the leaves in August and September. A decrease was obtained in six components in the leaves and three components in the stems, while increases were observed in one component in the leaves (10 kDa) and four components in the stems (10, 43, 50 and 65 kDa). One reason for the lowered cold-resistance in the bilberry caused by air pollutants could thus be the lowered concentrations of certain soluble cytoplasmic proteins that protect the plant against winter conditions.     

Decreased blood level of β2-microglobulin in the employees of a factory which produced polychlorinated biphenyls

Under the project of evaluating the health status of the employees of CHEMKO factory (East Slovakia) which produced PCBs between 1955 and 1985, the level of β₂-microglobulin (β₂-m) was measured in the serum of 242 subjects from CHEMKO (Group A) and two control groups from much less polluted areas: 1. 277 females from a small mountainous village (Group B), 2. 179 adults from the area of a large city of Ko ice (Group C). The level of thymidine kinase (TK) was measured in the groups A and B only. In addition, age-matched groups of 155 women each from all areas were evaluated. In both the whole group and the age-matched group from CHEMKO the level of β₂-m was significantly lower (P<0.001) than that in the respective control groups, while no difference was found in the level of TK. In conclusion, it is suggested that the decrease of β₂-m in CHEMKO employees might be related to the immunotoxic effects of organochlorines.