Effect of methyl mercuric chloride treatment on haematological characteristics and erythrocyte morphology of Swiss mice

Effects of methyl mercuric chloride (MMC) on the blood parameters of Swiss mice (Mus musculus) were studied. The mice received an initial dose of MMC (24 mg kg(-1) body wt) as intraperitoneal injection followed by a second similar dose on the 14th day of the first dose administration. Significant (p < or = 0.001) decreases in haemoglobin content, red blood cell (RBC) count and haematocrit value were observed in the MMC injected mice when compared to the control mice. The effect of the second dose was severe, after which no significant recovery in the values of these parameters was observed. The result also showed a high degree of mercury accumulation in the blood of the MMC exposed mice. Interesting features were marked in the erythrocyte morphology of the exposed mice. An initial shrinkage followed by swelling of the cells was observed after each injection. The outline of the exposed cells was irregular with beak like or small finger like projections. Rupturing and disintegration of the erythrocyte membrane, leading to erythrolysis, were also noticed.     

Dry aerial fallout of organochlorine insecticide residues in Delhi, India

Monitoring of airborne dust in Delhi during May to July 1985 revealed residues of DDT varying from 1.3 to 7.14 ng mg(-1) (4.06-22.31 ng m(-2) day(-1)) with an average of 3.32 ng mg(-1) (10.38 ng m(-2) day(-1)), and HCH which ranged from 0.46 to 2.35 ng mg(-1) (1.44-7.34 ng m(-2) day(-1)) with a mean of 1.16 ng mg(-1) (3.63 ng m(-2) day(-1)). The concentration of total DDT was almost 3 times greater than that of HCH.     

Radionuclides and the birds at Ravenglass

Since 1983, concern has been expressed about the apparent decline in numbers of birds in the Ravenglass estuary in west Cumbria, particularly of the black-headed gull colony on the Drigg dunes, and suggestions have been made that this decline might be due to excessive radiation in the birds' food and their general environment. Twelve species of marine invertebrates from Ravenglass, most of them known to be important foods for birds, were analysed, and further samples were taken from sites along the west Cumbrian coast. None of these samples showed excessive contamination with any of the radionuclides analysed. Analysis of a sample of bird carcasses from the areas showed oystercatchers (Haematopus ostralegus) and shelduck (Tadorna tadorna) to have some of the highest concentrations of (137)Cs in their tissues; yet their breeding success and populations were not affected. Black-headed gulls, on the other hand, were found to be feeding mainly inland, and were the least contaminated with radionuclides of all the birds at Ravenglass, yet this species and its breeding success were in decline. Calculations of the total dose equivalent rate to the whole body of the most contaminated black-headed gull amounted to 9.8 x 10(-4) mSvh(-1) (approximately equal to 8.4 x 10(-4) mGy h(-1), whole body absorbed dose rate), and the background exposure dose was of the order of 8.3 x 10(-4) mGy h(-1). As a minimum chronic dose of 1000 mGy day(-1) has been found necessary to retard growth of nestling birds, and 9600 mGy over 20 days of incubation to cause the death of 50% of embryos in black-headed gulls' eggs, the concentrations of radionuclides in the foods, body tissues and general environment were at least three orders of magnitude too low to have had any effect. The more likely cause of the desertion of the gullery was the combination of an uncontrolled fox population, the severest outbreak of myxomatosis amongst the rabbits since 1954 and the driest May-July period on record, all in the same year (1984).     

A natural analogue of high-pH cement pore waters from the Maqarin area of northern Jordan: Comparison of predicted and observed trace-element chemistry of uranium and selenium

Current design concepts for low-/intermediate-level radioactive waste disposal in many countries involve emplacement underground in a cementitious repository. The highly alkaline groundwaters at Maqarin, Jordan, are a good analogue for the cementitious pore waters that will be present within such a repository. A geochemical modelling study of these groundwaters has been carried out in order to test the applicability of equilibrium models in geochemical programs and their associated thermodynamic databases in such hyperalkaline conditions. This was achieved by comparison of elemental solubilities and speciations predicted by the programs with observations in the natural system. Five organisations took part in the study: AEA Technology, U.K.; Chalmers University of Technology, Sweden; MBT Tecnología Ambiental, Spain; Nagra, Switzerland; and SKB, Sweden. The modelling study was coordinated by the University of Berne.The results of the study showed good agreement between the predictions of the programs employed. Comparison of the observed solids with those predicted by the models has allowed limited validation of the databases. The results for U and Se are presented here.     

Characterization of trace organic contaminants in marine sediment from Yeongil Bay, Korea: 2. Dioxin-like and estrogenic activities

This study employed mechanism-specific in vitro bioassays to help characterize the occurrence and distribution of dioxin-like and estrogenic contaminants in sediment from Yeongil Bay, Korea. Approximately 85% of the sediments tested induced significant dioxin-like activity in the H4IIE-luc bioassay, while approximately 50% induced significant estrogenic activity in the MVLN bioassay. Instrumentally-derived estimates of 2,3,7,8-tetrachlorodibenzo-p-dioxin and 17beta-estradiol equivalents tended to underestimate the magnitude of response observed in the bioassays, suggesting that compounds detected by chemical analysis did not account for all the activity associated with Yeongil Bay sediments, or that non-additive interactions were occurring. The greatest dioxin-like and estrogenic activity was associated with the mid-polarity Florisil fractions (F2) expected to contain polycyclic aromatic hydrocarbons (PAHs) as well as chlorinated dioxins and furans. As in previous studies of Korean coastal sediment, more polar fractions (F3) generated more modest responses both in terms of magnitude and the number of samples responding.     

Genotypic variations in the accumulation of Cd, Cu, Pb and Zn exhibited by six commonly grown vegetables

Metal contaminants in garden and allotment soils could possibly affect human health through a variety of pathways. This study focused on the potential pathway of consumption of vegetables grown on contaminated soil. Five cultivars each of six common vegetables were grown in a control and in a soil spiked with Cd, Cu, Pb and Zn. Highly significant differences in metal content were evident between cultivars of a number of vegetables for several of the contaminants. Carrot and pea cultivars exhibited significant differences in accumulated concentrations of Cd and Cu with carrot cultivars also exhibiting significant differences in Zn. Distinctive differences were also identified when comparing one vegetable to another, legumes (Leguminosae) tending to be low accumulators, root vegetables (Umbelliferae and Liliaceae) tending to be moderate accumulators and leafy vegetables (Compositae and Chenopodiaceae) being high accumulators.     

Effect of farmyard manure application on boron adsorption-desorption characteristics of some soils

Boron (B) availability to crop plants depends on soil properties as well as management practices like liming, fertilization and use of organic manures. To assess the effect of farmyard manure (FYM) application on availability of added B, adsorption-desorption of B was investigated in five different soils receiving varying doses of FYM (0, 5 and 10 g FYM kg(-1) soil). Two surfaces Freundlich model was found best to account for B adsorption-desorption data of all soils. Application of FYM increased B adsorption capacities pertaining to low (K1) and high (K2) concentration ranges in all soils, except Soil C (Alfisol) having a pH of 9.8, in which the higher rate of FYM decreased the value of K2. Application of FYM did not change B desorption capacities of soils corresponding to low B concentration range (K(1)(1)) significantly, however, it increased B desorption capacity pertaining to high B concentration (K(1)(2)) in all soils, except Soils C (Alfisol) and E (Entisol) having pH of 9.8 and 5.1, respectively. Application of FYM increased the desorption slope factor applicable to low concentration range (1/n(1)(1)) in Soil A (Inceptisol), but decreased it in Soil E (Entisol). The 1/n(1)(2) (desorption slope factor applicable to high concentration range) decreased with FYM application in all soils except Soil E (Entisol), where it was increased. Boron desorption index (slope(ads)/slope(des)) decreased with FYM application in low B concentration range, but increased in high concentration range for all soils except soil E (Entisol, pH 5.1), in which a reverse trend was observed. Application of FYM increased the retention of added B in soils and may help reducing the leaching losses.     

Bagasse-assisted bioremediation of ammonia from shrimp farm wastewater.

Development of new economically feasible ecofriendly products from agricultural wastes or byproducts for shrimp farm wastewater treatment is the objective of our continued research. Ammonia is a nitrogenous toxicant, which is commonly found in wastewater from shrimp farms. In the present study, we explored the possibility of the use of simply and inexpensively prepared bagasse products so that this abundant crop byproduct could be used to remove ammonia from shrimp farm wastewater. Bagasse, a natural highly fibrous lignocellulosic byproduct of sugarcane, was converted into five different products. Experimental results have shown that ammonia is efficiently removed from wastewater by four bagasse products with a dose of 1 to 6 g/L within 24 hours. The effect of bagasse products on other water quality parameters and growth kinetics of biofilm bacteria onto bagasse fiber have also been studied. Efficacies of products were compared by using statistical analysis. Products developed from bagasse are useful and economical.