Measured gas emissions from four landfills in south africa and some implications for landfill design and methane recovery in semi-arid climates.

The magnitude of annual global emissions of methane from municipal solid waste landfills without landfill gas control systems implies that these landfills are significant contributors to the atmospheric load of greenhouse gases. There have been a number of field studies undertaken internationally to measure actual fluxes of methane and carbon dioxide from landfills, with a view to corroborating modelled predictions of the contribution of landfills to the global greenhouse gas budget. The vast majority of these studies have been undertaken in more temperate climates and in developed countries. This paper reports a study of landfill gas emissions from four large landfills located in the semi-arid interior of South Africa. A static accumulation chamber was used and measurements were made at each site over a period of two to three days. The results were analysed by three different methods, all of them leading to the same general conclusion that landfill gas emission rates were lower than expected. A common conclusion based on results from all four sites was that capping of landfills in semi-arid climates with low permeability covers would probably significantly retard the already low rate of waste degradation and thus gas generation. While this may be regarded as advantageous in the short term, it cannot be relied upon in perpetuity as clayey landfill covers will inevitably desiccate and crack in a semiarid environment. In addition, reasonable after-care periods for such landfills are likely to extend well beyond the currently stipulated 30-year period, and efforts to encourage energy recovery from landfills may be hampered because gas generation rates decrease as the waste dries out under conditions of minimal recharge from precipitation. A landfill cover that allows small amounts of percolation of rainfall into the waste may therefore in fact be beneficial in semiarid climates, although care would need to be taken to carefully regulate this infiltration.     

Methyl 2-(methylthio)benzoate: the unique sulfur-containing sex pheromone of<Emphasis Type="Italic"> Phyllophaga crinita</Emphasis>

The female-produced sex pheromone of Phyllophaga crinita (Burmeister) (Coleoptera: Scarabaeidae: Melolonthinae; the adult has no common name) is identified as methyl 2-(methylthio)benzoate. This is the first identification of a sulfur-containing, long-distance, female-produced sex attractant from any insect taxa. The root-feeding larvae of this species are serious pests in many crops in Texas and Mexico. In field tests, many P. crinita males were captured in traps baited with the authentic compound. Interestingly, a heteroatom analog, methyl 2-methoxybenzoate, also captured P. crinita males, but only at a dose 10,000 times higher than the lowest tested dose of the authentic pheromone.In memory of M.G. Villani, who died on 15 May 2001. His enthusiasm for soil-dwelling scarab beetles and contributions to their research are greatly missed.     

Cystic fibrosis prenatal diagnosis: Confirmation of an equivocal microvillar enzyme result by direct analysis of the common gene mutation

A child was tentatively diagnosed as having cystic fibrosis, based on neonatal presentation with severe gastrointestinal complications; the diagnosis was not confirmed biochemically and no tissues were available for DNA analysis. The mother presented in her subsequent pregnancy, and microvillar enzyme analysis of cell-free amniotic fluid at both 18 and 20 weeks gestation gave equivocal results. The pregnancy was terminated voluntarily because of a trend towards abnormal enzyme assay results on the second amniocentesis. Retrospectively, fetal tissues were found to be homozygous for the most common mutation of the cystic fibrosis gene (AF₅₀₈), which confirmed the prenatal assessment and suggested that the first infant of the couple was probably also affected by the disease.     

Prenatal ultrasound diagnosis of gastric outlet obstruction due to a pyloric web

Gastrointestinal tract atresia has an incidence of 1 in 10 000 live-births, while gastric outlet obstruction comprises only approximately 1 per cent of these malformations. A prenatally diagnosed case is described, followed by a discussion regarding the diagnosis and possible associated abnormalities.     

Questions on the mechanism of temperature adaptation in marine phytoplankton

The rate of light-saturated photosynthesis in 3 marine algae [Phaeodactylum tricornutum Bohlin, Nitzschia closterium (Ehrenberg) Smith and Dunaliella tertiolecta Butcher] varies during growth in batch culture. The photosynthetic rare declines most rapidly during growth at the higher temperatures. Because of these changes in photosynthesis rate, the previously reported enhanced photosynthetic abilities caused by growth at lower temperatures (generally interpreted as evidence for higher enzyme levels) can only be observed when measurements are made late in the exponential phase or after the onset of the stationary phase of growth. When allowance is made for the earlier peak of photosynthetic ability in cultures growing at higher temperatures, there is no evidence for adaptation to lower temperatures being caused by increased levels of the enzymes required for carbon-dioxide fixation. When the changes due to growth in batch culture are taken into account, certain effects of temperature can be recognized. the dry weight: chlorophyll ratio of all 3 algae increases with decreasing growth temperatures. For P. tricornutum and N. closterium, growth at lower temperatures reduces the cellular content of chlorophyll a, but has little effect on the chlorophyll content of D. tertiolecta. The dry weight: cell-number ratio of D. tertiolecta and P. tricornutum increases with lower growth temperatures, but growth temperature has little effect on the cell mass of N. closterium. Growth of the 3 algae at lower temperatures does not increase their ability to photosynthesize at these lower temperatures. Rather, it reduces their ability to assimilate carbon dioxide at the higher temperatures.     

Sexual selection and species recognition in the pygmy swordtail, Xiphophorus pygmaeus: conflicting preferences

Sexual selection and species recognition play important roles in mate choice; however, sexual selection preferences may overlap with traits found in heterospecifics, producing a conflict between sexual selection and species recognition. We examined female preferences in Xiphophorus pygmaeus for male traits that could provide both types of information to determine how females use multiple cues when preferences for these cues would conflict. We also examined X. pygmaeus behavior in the field to determine if females have the opportunity to choose mates. As no male-male competition was observed in the field, and females occasionally chased males from feeding areas, females apparently have the opportunity to exercise mate choice in their natural habitat. In the laboratory, female X. pygmaeus used body size as a sexual selection cue, preferring large heterospecifics (X. cortezi) to small conspecifics. Females also preferred barless X. cortezi over barred X. cortezi when males were size matched. Because X. pygmaeus males do not have bars, this preference suggests that X. pygmaeus females use vertical bars in species recognition, and that large body size and vertical bars are conflicting cues. However, X. pygmaeus females did not have a preference for males of either species when sexual selection and species recognition cues were presented concurrently. This result was surprising, because preferences for species recognition cues are often assumed to be stronger than sexual selection cues. We suggest that females may be using additional species-specific cues in mate choice to prevent hybridization.     

An apparatus for measuring microbial growth or survival in the marine environment

The apparatus consists of a glass tube closed at the top and with a bacteria-proof sinter-glass filter at the lower end. This is moored below the surface of the sea after inoculation with the organism under examination. Water enters and leaves the tube due to the hydrostatic pressure differential created by tides and waves; this gives a desirable rate of exchange of sea water withouth allowing the entry of any foreign organisms. After the period of immersion, the amount of growth or survival of the organism is estimated.     

Lessons from an Evaluation of a Boater Outreach Program for Manatee Protection

Watercraft collisions account for 25–30% of manatee deaths annually in Florida. Education and outreach interventions for boaters are strategies for reducing manatee mortality. We evaluated the effectiveness of the Manatee Watch program by surveying primary boat users whose boats were approached by Manatee Watch. We compared the attitudes, knowledge, and behavioral intentions of boaters who received Manatee Watch materials with a control group of boaters observed by the Florida Marine Research Institute in Tampa Bay during 1999–2001. Results of the 51-item telephone survey with 499 boaters indicated that the Manatee Watch intervention had little effect on boater’s attitudes, knowledge, and behaviors regarding manatees. However, individual attitude scores were positively correlated with safe boating behaviors in shallow waters including maintaining a slower speed and watching out for manatees. Overall knowledge about manatees was correlated with one manatee-safe boating behavior. To improve efficacy, educators should (a) incorporate evaluation into the planning stages of program development; (b) target messages to influence boaters’ attitudes toward manatees and ecosystem health, and their feelings of ownership and empowerment; (c) facilitate active participation of the boaters; and (d) increase the duration and variety of intervention.