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81.
The influence of time on lead toxicity and bioaccumulation determined by the OECD earthworm toxicity test 总被引:5,自引:0,他引:5
Internationally agreed standard protocols for assessing chemical toxicity of contaminants in soil to worms assume that the test soil does not need to equilibrate with the chemical to be tested prior to the addition of the test organisms and that the chemical will exert any toxic effect upon the test organism within 28 days. Three experiments were carried out to investigate these assumptions. The first experiment was a standard toxicity test where lead nitrate was added to a soil in solution to give a range of concentrations. The mortality of the worms and the concentration of lead in the survivors were determined. The LC50s for 14 and 28 days were 5311 and 5395 microgPb g(-1)soil respectively. The second experiment was a timed lead accumulation study with worms cultivated in soil containing either 3000 or 5000 microgPb g(-1)soil. The concentration of lead in the worms was determined at various sampling times. Uptake at both concentrations was linear with time. Worms in the 5000 microg g(-1) soil accumulated lead at a faster rate (3.16 microg Pb g(-1)tissue day(-1)) than those in the 3000 microg g(-1) soil (2.21 microg Pb g(-1)tissue day(-1)). The third experiment was a timed experiment with worms cultivated in soil containing 7000 microgPb g(-1)soil. Soil and lead nitrate solution were mixed and stored at 20 degrees C. Worms were added at various times over a 35-day period. The time to death increased from 23 h, when worms were added directly after the lead was added to the soil, to 67 h when worms were added after the soil had equilibrated with the lead for 35 days. In artificially Pb-amended soils the worms accumulate Pb over the duration of their exposure to the Pb. Thus time limited toxicity tests may be terminated before worm body load has reached a toxic level. This could result in under-estimates of the toxicity of Pb to worms. As the equilibration time of artificially amended Pb-bearing soils increases the bioavailability of Pb decreases. Thus addition of worms shortly after addition of Pb to soils may result in the over-estimate of Pb toxicity to worms. The current OECD acute worm toxicity test fails to take these two phenomena into account thereby reducing the environmental relevance of the contaminant toxicities it is used to calculate. 相似文献
82.
The effects of increased trophic complexity, through the addition of predatory notonectids (Anisops deanei), on temporary pond microcosms used for aquatic toxicity testing were studied. Replicate microcosms were established using sediment from a dried temporary pond, and treated with one of four concentrations of the organochlorine pesticide endosulfan (0, 1, 10 or 50 microg/L), in the presence or absence of six A. deanei. The tanks were sampled regularly for nine weeks following the addition of the predators and the entire contents of each tank counted after 12 weeks. Analysis using non-metric multidimensional scaling (MDS) and non-parametric MANOVA showed that both Anisops and endosulfan at concentrations >10 microg/L significantly altered community structure. However, an interaction between the effects of Anisops and the effects of endosulfan was not detected. The addition of Anisops did not increase the variability of response and thus did not reduce the sensitivity of the test method. 相似文献
83.
Thomas Merckx Ruth E. Feber Philip Riordan Martin C. Townsend Nigel A.D. Bourn Mark S. Parsons David W. Macdonald 《Agriculture, ecosystems & environment》2009,130(3-4):177-182
How best to optimize the biodiversity gain from agri-environment schemes (AES) has recently been identified as a key policy-relevant question. Here, the effects of two features of lowland agricultural landscapes on the abundance and diversity of larger moths are contrasted. Although both features bring about positive effects, hedgerow trees have a larger impact than 6 m wide grassy field margins. Whilst AES payments are given to create and maintain grass margins, no financial reward is currently offered for the retention of hedgerow trees. Furthermore, it was only in areas where the amount of land under AES was experimentally increased, by targeting farmers, that the presence of hedgerow trees resulted in a substantially higher abundance (+60%) and diversity (+38%) of moths. Thus, by using larger moths as bio-indicators of landscape-scale quality, it is demonstrated that improvements to the cost-effectiveness of AES could be achieved, firstly, by providing more appropriate financial rewards to farmers for different landscape features, and secondly, through landscape-scale targeting of farmers to encourage participation in AES. 相似文献
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Invertebrates containing endosymbiotic dinoflagellate algae (zooxanthellae) retain excretory nitrogen, and many are able to take up ammonium from the surrounding seawater. However, the site of assimilation and role of nitrogen recycling between symbiont and host remains unclear. In the present study, ammonium uptake by the symbiotic sea anemone Anemonia viridis (Forskål) was examined by following the pathway of assimilation using 15N-enriched ammonium. Since zooxanthellae became enriched with 15N from ammonium at up to 17 times the rate of the host, they appear to be the primary site of assimilation. In the light, the rate of zooxanthellae enrichment at 20?M was twice that at 10?M, whereas the rate of host enrichment was not significantly affected by ammonium concentration. When anemones were incubated with [15N]ammonium in the dark, after 12?h without light the rate of enrichment was lowered in both zooxanthellae and host. However, while the enrichment of the host was significantly reduced when the light level was lowered from 300 to 150?μmol photons m?2?s?1, zooxanthellae enrichment was unchanged. Low molecular weight material from the zooxanthellae became enriched at 20 times the rate of that from the host, and enrichment was detected in the amino acids glutamate, glutamine, aspartate, alanine, glycine, phenylalanine, threonine, valine, tyrosine, and leucine from zooxanthellae. In the zooxanthellae, amino acids accounted for 65% of the total enrichment of low molecular weight material. Of the amino acids detected in zooxanthellae, over 90% of the enrichment was accounted for by glutamate, glutamine and aspartate. The enrichment of the amide group of glutamine was greater than that of the amine group of glutamate or glutamine, consistent with the glutamine synthetase/glutamine 2-oxoglutarate amidotransferase cycle as the mechanism of ammonium assimilation. To examine the flux of 15N from zooxanthellae to host, anemones were pulse-labelled with [15N]ammonium and then transferred to an unlabelled chase. Over a 2?h period there was no evidence for a flux of nitrogen from zooxanthellae to host. However, during the chase period, the enrichment of low molecular weight material declined and that of high molecular weight material increased in both zooxanthellae and host, indicating that protein was synthesized using 15N from ammonium in both components of the symbiosis. Again by using a pulse-chase system, it was found that glutamate was metabolised most rapidly by zooxanthellae, followed by (in order of decreasing rate of turnover) aspartate, alanine, glycine and valine (no data are available for glutamine). Unlike these amino acids, nitrogen was transferred to the essential amino acids phenylalanine and threonine, increasing their enrichment during the chase period. While recycled nitrogen is clearly important to this symbiosis, the mechanism by which it is cycled remains to be resolved. 相似文献
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89.
Carlos Rozas Alberto de la Fuente Hugo Ulloa Peter Davies Yarko Niño 《Environmental Fluid Mechanics》2014,14(4):849-871
Lake Villarrica, located in south central Chile, has a maximum depth of 167 m and a maximum fetch of about 20 km. The lake is monomictic, with a seasonal thermocline located at a depth of approximately 20 m. Field data show the presence of basin-scale internal waves that are forced by daily winds and affected by Coriolis acceleration. A modal linear and non-linear analysis of internal waves has been used, assuming a two-layer system. The numerical simulations show good agreement with the internal wave field observations. The obtained modes were used to study the energy dissipation within the system, which is necessary to control the amplitude growth. Field data and numerical simulations identify (1) the occurrence of a horizontal mode 1 Kelvin wave, with a period of about a day that coincides with the frequency of daily winds, suggesting that this mode of the Kelvin waves is in a resonant state (subject to damping and controlled by frictional effects in the field) and (2) the presence of higher-frequency internal waves, which are excited by non-linear interactions between basin-scale internal waves. The non-linear simulation indicates that only 10 % of the dissipation rate of the Kelvin wave is because of bottom friction, while the rest 90 % represents the energy that is radiated from the Kelvin wave to other modes. Also, this study shows that modes with periods between 5 and 8 h are excited by non-linear interactions between the fundamental Kelvin wave and horizontal Poincaré-type waves. A laboratory study of the resonant interaction between a periodic forcing and the internal wave field response has also been performed, confirming the resonance for the horizontal mode 1 Kelvin wave. 相似文献
90.
David E. Jennings Joseph D. DiBattista Kristine L. Stump Nigel E. Hussey Bryan R. Franks R. Dean Grubbs Samuel H. Gruber 《Journal of Coastal Conservation》2012,16(3):405-428
Coastal biodiversity is threatened worldwide by both direct and indirect anthropogenic activities. To more effectively manage and protect coastal biodiversity, accurate assessments of genetic, species, and ecosystem level diversity are required. We present the results from an assessment of the aquatic species diversity of a small (3?km2), shallow, mangrove-fringed Bahamian lagoon (the North Sound) subject to ongoing anthropogenic development. The assessment was conducted through a collation of field observations and data in published literature. We found that eight angiosperm species, 30 macroalgal species, and 370 animal species (including 95 fishes, 69 arthropods, 56 birds, and 45 mollusks) were documented within the lagoon. At least 11 of these species are of conservation concern, such as the critically endangered smalltooth sawfish (Pristis pectinata) and hawksbill turtle (Eretmochelys imbricata). Comparisons of community similarity indicated that the North Sound has a relatively distinct fauna and flora, but available data suggest that the species found there are most similar to those found in nearby habitats in Cuba. The lagoon forms a key nursery habitat for many species, including lemon sharks (Negaprion brevirostris), Caribbean spiny lobsters (Panulirus argus), and queen conch (Strombas gigas). Recently, the lagoon was included as part of a new marine protected area (MPA), but much of the habitat has already experienced considerable anthropogenic disturbance and the MPA boundaries have yet to be established. We have therefore analyzed the lagoon biodiversity and expect the data presented here to serve as a baseline for future comparisons. 相似文献