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91.
Brigitte M. Manship Anthony J. Walker Lucy A. Jones Angela J. Davies 《Marine Biology》2008,153(3):473-482
Juveniles of Paragnathia formica Hesse (Isopoda; Gnathiidae) are haematophagous ectoparasites, feeding on fish blood which supplies the nutrients for their
development through growth and moulting, and the subsequent survival and reproduction of the free-living adults. Little is
known of the mechanisms of digestion in juvenile gnathiids, and biochemical studies on the digestive tract of these interesting
estuarine isopods have not been undertaken previously. Here, functionally active cathepsin-like cysteine proteinases are identified
in the digestive system of juvenile praniza (fed) and zuphea (unfed) forms. The physiological pH of the digestive tract and
the optimum proteolytic activities detected in praniza 3 homogenates using the cathepsin B/L, cathepsin B, and cathepsin H
fluorogenic substrates, N-Carbobenzoxy-Phe-Arg-4-methoxy-2-naphthylamine (Z-phe-arg-MNA), N-Carbobenzoxy-Arg-Arg-4-methoxy-2-naphthylamine (Z-arg-arg-MNA) and Arg-4-methoxy-2-naphthylamine (H-arg-MNA), respectively, are in the acidic range (pH 5.8–6.7). Inhibition profiles against Z-phe-arg-MNA and Z-arg-arg-MNA using the cathepsin B inactivator urea, and cysteine proteinase inhibitors, support the presence of cathepsin
L- and B-like enzymes. These proteolytic activities are 10–50 times higher in homogenates of praniza 3 compared with zuphea
3 forms. Histochemistry of praniza 3 sections reveals that the predominant enzyme activity towards Z-phe-arg-MNA is limited to the digestive glands during early and mid stages of digestion; later, this activity appears in
the lining of the anterior hindgut. Moreover, activity towards Z-arg-arg-MNA is generally restricted to the digestive glands, and only occasionally present in the anterior hindgut. In conclusion,
the digestive glands are the main site of cathepsin-like cysteine proteinase activities in P. formica juveniles; these enzymes appear to be important to the digestion of host fish blood enabling development through to the free-living
adult. 相似文献
92.
Elva J. H. Robinson Thomas O. Richardson Ana B. Sendova-Franks Ofer Feinerman Nigel R. Franks 《Behavioral ecology and sociobiology》2009,63(5):779-636
Ant colonies are factories within fortresses (Oster and Wilson 1978). They run on resources foraged from an outside world fraught with danger. On what basis do individual ants decide to leave the safety of the nest? We investigated the relative roles of social information (returning nestmates), individual experience and physiology (lipid stores/corpulence) in predicting which ants leave the nest and when. We monitored Temnothorax albipennis workers individually using passive radio-frequency identification technology, a novel procedure as applied to ants. This method allowed the matching of individual corpulence measurements to activity patterns of large numbers of individuals over several days. Social information and physiology are both good predictors of when an ant leaves the nest. Positive feedback from social information causes bouts of activity at the colony level. When certain social information is removed from the system by preventing ants returning, physiology best predicts which ants leave the nest and when. Individual experience is strongly related to physiology. A small number of lean individuals are responsible for most external trips. An individual’s nutrient status could be a useful cue in division of labour, especially when public information from other ants is unavailable. 相似文献
93.
R. J. Davies‐Colley D. G. Smith 《Journal of the American Water Resources Association》2001,37(5):1085-1101
ABSTRACT: Suspended sediment causes a range of environmental damage, including benthic smothering, irritation of fish gills, and transport of sorbed contaminants. Much of the impact, while sediment remains suspended, is related to its light attenuation, which reduces visual range in water and light availability for photosynthesis. Thus measurement of the optical attributes of suspended matter in many instances is more relevant than measurement of its mass concentration. Nephelometric turbidity, an index of light scattering by suspended particles, has been widely used as a simple, cheap, instrumental surrogate for suspended sediment, that also relates more directly than mass concentration to optical effects of suspended matter. However, turbidity is only a relative measure of scattering (versus arbitrary standards) that has no intrinsic environmental relevance until calibrated to a ‘proper’ scientific quantity. Visual clarity (measured as Secchi or black disc visibility) is a preferred optical quantity with immediate environmental relevance to aesthetics, contact recreation, and fish habitat. Contrary to common perception, visual clarity measurement is not particularly subjective and is more precise than turbidity measurement. Black disc visibility is inter‐convertible with beam attenuation, a fundamental optical quantity that can be monitored continuously by beam transmissometry. Visual clarity or beam attenuation should supplant nephelometric turbidity in many water quality applications, including environmental standards. 相似文献
94.
Elizabeth A. Langridge Ana B. Sendova-Franks Nigel R. Franks 《Behavioral ecology and sociobiology》2008,62(3):447-456
Certain groups of organisms are capable of improving their collective performance with experience. In a recent study, we demonstrated
that, over successive emigrations, colonies of the ant Temnothorax albipennis are able to improve their collective performance by reducing the time taken to complete an emigration (Langridge et al.,
Behav Ecol Sociobiol 56:523–529, 2004). In this paper, by recording the performance of individually marked workers during
repeated emigrations, we were able to analyse some of the ways in which time gains are achieved. We found that: (1) those
transporters that also transported in the preceding emigration began to transport earlier in the current emigration and, in
the majority of emigrations, transported more items than those transporters that had not transported in the preceding emigration;
(2) the time that elapsed before the first item was transported into the new nest reduced over successive emigrations, and
this first item was, in the majority of emigrations, carried by a transporter that had also transported in the preceding emigration;
(3) the number of adults that were transported reduced over successive emigrations. Our results strongly suggest that the
behaviour of transporters that also transported in a preceding emigration may be modified as a result of their experience
and that, consequently, their efforts in the next emigration make a major contribution to the improved performance of the
colony as a whole. 相似文献
95.
96.
The photocatalytic degradation of dimethyl phthalate (DMP) in aqueous TiO2 suspension under UV illumination has been investigated using oxygen (O2) and ferrate (Fe(VI)) as electron acceptors. The experiments demonstrated that Fe(VI) was a more effective electron acceptor than O2 for scavenging the conduction band electrons from the surface of the catalyst. Some major intermediate products from DMP degradation were identified by HPLC and GC/MS analyses. The analytical results identified dimethyl 3-hydroxyphthalate and dimethyl 2-hydroxyphthalate as the two main intermediate products from the DMP degradation in the TiO2–UV–O2 system, while in contrast phthalic acid was found to be the main intermediate product in the TiO2–UV–Fe(VI) system. These findings indicate that DMP degradation in the TiO2–UV–O2 and TiO2–UV–Fe(VI) systems followed different reaction pathways. An electron spin resonance analysis confirmed that hydroxyl radicals existed in the TiO2–UV–O2 reaction system and an unknown radical species (most likely an iron–oxo species) is suspected to exist in the TiO2–UV–Fe(VI) reaction system. Two pathway schemes of DMP degradation in the TiO2–UV–O2 and TiO2–UV–Fe(VI) reaction systems are proposed. It is believed that the radicals formed in the TiO2–UV–O2 reaction system preferably attack the aromatic ring of the DMP, while in contrast the radicals formed in the TiO2–UV–Fe(VI) reaction systems attack the alkyl chain of DMP. 相似文献
97.
Konstantinov A Bejan D Bunce NJ Chittim B McCrindle R Potter D Tashiro C 《Chemosphere》2008,72(8):1159-1162
Electrochemical debromination of the commercial decabromodiphenyl ether flame retardant DE-83 in partly aqueous tetrahydrofuran (THF) solution gave lower brominated congeners by sequential loss of bromine atoms. Hydrodebromination was most facile for the most heavily brominated congeners. It involves initial electron transfer and proton transfer from water, rather than hydrogen atom abstraction from THF, as shown by experiments with deuterated water. The product distribution from electrolysis involves preferential loss of bromine meta- and para- to the ether linkage, comparable with the products of metabolism of BDE-209 in various organisms. Significantly, the environmentally relevant congeners BDE-47, BDE-99, and BDE-154 were not major products of debromination of BDE-209 by the electron transfer mechanism. 相似文献
98.
Yates K Davies I Webster L Pollard P Lawton L Moffat C 《Journal of environmental monitoring : JEM》2007,9(10):1116-1121
Silicone rubber sheeting can be used as a passive sampling device for hydrophobic organic contaminants in the environment to determine the available concentrations in water and sediments. Reliable sampler-water partition coefficients are required to determine the sampling rates and the dissolved contaminant concentrations in water and in sediment pore water. Log partition coefficients (logK(sr,w)) for silicone rubber-water have been estimated for 32 polycyclic aromatic hydrocarbons (PAHs), 2 deuterated PAH analogues and 32 chlorobiphenyls (CBs) using the cosolvent method, with methanol as cosolvent. Strong linear relationships were found with literature values for the corresponding log octanol-water partition coefficients (logK(ow)) for both CBs and PAHs, confirming that partitioning into the silicone rubber is strongly determined by the hydrophobicity of the compounds, which suggests logK(ow) is a good predictor of logK(sr,w) and that absorption is the main mechanism for accumulation of analytes into the silicone rubber polymer. 相似文献
99.
Biosensors for detection of mercury in contaminated soils 总被引:1,自引:0,他引:1
Bontidean I Mortari A Leth S Brown NL Karlson U Larsen MM Vangronsveld J Corbisier P Csöregi E 《Environmental pollution (Barking, Essex : 1987)》2004,131(2):255-262
Biosensors based on whole bacterial cells and on bacterial heavy metal binding protein were used to determine the mercury concentration in soil. The soil samples were collected in a vegetable garden accidentally contaminated with elemental mercury 25 years earlier. Bioavailable mercury was measured using different sensors: a protein-based biosensor, a whole bacterial cell based biosensor, and a plant sensor, i.e. morphological and biochemical responses in primary leaves and roots of bean seedlings grown in the mercury-contaminated soil. For comparison the total mercury concentration of the soil samples was determined by AAS. Whole bacterial cell and protein-based biosensors gave accurate responses proportional to the total amount of mercury in the soil samples. On the contrary, plant sensors were found to be less useful indicators of soil mercury contamination, as determined by plant biomass, mercury content of primary leaves and enzyme activities. 相似文献
100.
Helen C. Wheeler Dominique Berteaux Chris Furgal Kevin Cazelles Nigel G. Yoccoz David Grémillet 《Conservation biology》2019,33(4):861-872
For effective monitoring in social–ecological systems to meet needs for biodiversity, science, and humans, desired outcomes must be clearly defined and routes from direct to derived outcomes understood. The Arctic is undergoing rapid climatic, ecological, social, and economic changes and requires effective wildlife monitoring to meet diverse stakeholder needs. To identify stakeholder priorities concerning desired outcomes of arctic wildlife monitoring, we conducted in-depth interviews with 29 arctic scientists, policy and decision makers, and representatives of indigenous organizations and nongovernmental organizations. Using qualitative content analysis, we identified and defined desired outcomes and documented links between outcomes. Using network analysis, we investigated the structure of perceived links between desired outcomes. We identified 18 desired outcomes from monitoring and classified them as either driven by monitoring information, monitoring process, or a combination of both. Highly cited outcomes were make decisions, conserve, detect change, disseminate, and secure food. These reflect key foci of arctic monitoring. Infrequently cited outcomes (e.g., govern) were emerging themes. Three modules comprised our outcome network. The modularity highlighted the low strength of perceived links between outcomes that were primarily information driven or more derived (e.g., detect change, make decisions, conserve, or secure food) and outcomes that were primarily process driven or more derived (e.g., cooperate, learn, educate). The outcomes expand monitoring community and disseminate created connections between these modules. Key desired outcomes are widely applicable to social–ecological systems within and outside the Arctic, particularly those with wildlife subsistence economies. Attributes and motivations associated with outcomes can guide development of integrated monitoring goals for biodiversity conservation and human needs. Our results demonstrated the disconnect between information- and process-driven goals and how expansion of the monitoring community and improved integration of monitoring stakeholders will help connect information- and process-derived outcomes for effective ecosystem stewardship. 相似文献