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Cellular damage of plant pathogenic fungi by antifungal compounds produced by Bacillus spp. isolates
Estibaliz Sansinenea Mauro Almaraz Maria Dolores Ramírez Aurelio Ortiz 《Chemistry and Ecology》2016,32(8):722-732
The use of microorganisms as biological control agents (BCAs) has become an effective alternative to chemical means of controlling plant pathogens. The antagonistic and inhibitory activity of 71 Bacillus spp. strains, which were isolated from different Mexican sites, were tested against several phytopathogen fungi, Fusarium oxysporum, Fusarium equiseti, Fusarium avenaceum, Bipolaris spp. and Alternaria spp. From the antagonism study, the strain ELI149 showed a marked inhibition of growth against all tested fungi; therefore crude metabolites from this strain were extracted using ethyl acetate and amberlite resin and probed against the same fungi as well as strains of Mucor sp., Penicillium spp. and Paecilomyces spp. The results indicated that amberlite was more suitable for extraction of secondary metabolites with antifungal activity. Finally, observation of cell damage in the tested pathogenic fungi showed marked morphological changes on reproductive structures in all tested fungi indicating that antibiosis was the mechanism of the antagonistic effect. These results suggest that metabolites from the Bacillus strains have a wide spectrum of antibiotic activities, which can be used as biocontrol agents for controlling fungal plant diseases of agricultural importance. 相似文献
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J.?D.?Qiao S.?K.?DelavanEmail author R.?I.?Nokes D.?R.?Plew 《Environmental Fluid Mechanics》2016,16(5):1021-1041
Laboratory experiments are conducted to quantify the mean flow structure and turbulence properties downstream of a spanwise suspended linear array in a uniform ambient water flow using Particle Tracking Velocimetry. Eighteen experimental scenarios, with four depth ratios (array depth to water column depth) of 0.35, 0.52, 0.78, and 0.95 and bulk Reynolds number (length scale is the array depth) from 11,600 to 68,170, are investigated. Three sub-layers form downstream of the array: (1) an internal wake zone, where the time-averaged velocity decreases with increasing distance downstream, (2) a shear layer which increases in vertical extent with increasing distance downstream of the array, and the rate of the increase is independent of the bulk Reynolds number or the depth ratio, and (3) an external wake layer with enhanced velocity under the array. The location of the shear layer is dependent on the depth ratio. The spatially averaged and normalized TKE of the wake has a short production region, followed by a decay region which is comparable to grid turbulence decay and is dependent on the depth ratio. The results suggest that the shear layer increases the transfer of horizontal momentum into the internal wake zone from the fluid outside of the array and that the turbulence in the internal wake zone can be modeled similarly to that of grid turbulence. 相似文献
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Regional ecosystem monitoring is a central form of knowledge sharing and collaboration amongst scientists and decision makers on environmental health, land use change, and science-policy development. Despite the proliferation of such research networks on long-term monitoring on many continents, little has been achieved in Africa. This study aims to assess and examine the spatiotemporal trend and categorical patterns in ecosystem monitoring-related research in Africa for the benefits of conserving biodiversity and sustaining natural resource sectors for well-being and livelihood security, environmental planning, and ecological stewardship. A systematic review was conducted using bibliometric tools. Based on a set of search terms and peer-reviewed publications retrieved from various ecosystem monitoring networks and journal databases, further analysis was conducted using social network approaches, mapping tools, and content analysis. About 1442 scientific publications on ecosystem monitoring and related research were documented from 1987 to 2014 mostly published in English. The number of publication increased progressively since 1992 after the Convention on Biodiversity was signed and this trend peaked till 2008. South African Journal of Science was the most leading journal and Nature the most cited. Internationally coauthored and collaborative articles represented majority of the findings with the United Kingdom at the central position in the research network due to colonial relationships. Regional collaboration amongst countries is limited owing to language barriers and other institutional constraints such as funding and short-term projects. These findings have implication for prioritizing national and regional policies toward biodiversity science and its contribution to human well-being, food security, and global change responses. 相似文献
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Kyle E. MURRAY Erin I. Manitou-ALVAREZ Enos C. INNISS Frank G. HEALY Adria A. BODOUR 《Frontiers of Environmental Science & Engineering》2015,9(1):39
Microorganisms are ubiquitous in natural environments and in water supply infrastructure including groundwater wells. Sessile-state microorganisms may build up on well surfaces as biofilms and, if excessive, cause biofouling that reduces well productivity and water quality. Conditions can be improved using biocides and other traditional well rehabilitation measures; however, biofilm regrowth is inevitable given the continuous introduction of microorganisms from the surrounding environment. Alternative and less invasive well maintenance approaches are desirable for reducing biofilm densities while also minimizing harmful disinfection-by-products. The primary objective of this research was to evaluate effectiveness of alternative treatments for inactivating microorganisms comprising biofilms. A novel approach was designed for in situ growth of biofilms on steel coupons suspended from ‘chandeliers’. After more than 100 days of in situ growth, biofilms were harvested, sampled, and baseline biofilm densities quantified through cultivation. Ultraviolet-C (UV-C) and oxidative treatments including hydrogen peroxide (H2O2), ozone (O3) and mixed oxidants were then applied to the biofilms in laboratory-scale treatments. Microbial inactivation was assessed by comparing treated versus baseline biofilm densities. H2O2 was the most effective treatment, and decreased density below baseline by as much as 3.1 orders of magnitude. Mixed oxidants were effective for the well having a lower density biofilm, decreasing density below baseline by as much as 1.4 orders of magnitude. Disparity in the response to treatment was apparent in the wells despite their spatial proximity and common aquifer source, which suggests that microbiological communities are more heterogeneous than the natural media from which they originate. 相似文献
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Chronologies are developed from the otolith growth-increment widths of adult rock flathead (Platycephalus laevigatus) and longhead flathead (Leviprora inops) collected from three inter-connected embayments in temperate south-western Australia. Marginal increment trends on otoliths, in combination with the dendrochronological technique of crossdating, provide strong evidence that an opaque zone is formed annually in the otoliths of both species. Increment widths between opaque zones in the otoliths of individuals of P. laevigatus (maximum age 20 years) and L. inops (maximum age 21 years) were synchronised within and between species. The correlation between the two master otolith chronologies, i.e. mean standardised increment width for each year, was highly significant (P < 0.01). The master otolith chronology for each species was positively correlated with mean sea surface temperatures from 1 July to 30 June, which encompassed the austral summer when growth is typically greatest, and with mean monthly sea surface temperature between September and March. Leeuwin Current strength, which influences the biotic characteristics of some marine species on the south coast, has little influence on the pattern of otolith growth, presumably because the inter-connected embayments from which fish were obtained were partially enclosed. On the basis of the above data and the geographical distributions of P. laevigatus and L. inops, it is proposed that, while both species would grow faster with increasing temperature, the temperatures on the south coast are closer to the optimum for the former species. 相似文献
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Michelle T. Schärer Michael I. Nemeth Timothy J. Rowell Richard S. Appeldoorn 《Marine Biology》2014,161(1):141-147
Passive acoustic and synchronous video recordings were made at two spawning aggregation sites to study the sounds associated with reproductive behaviors of Mycteroperca bonaci. A characteristic sound was produced during courtship displays involving behaviors commonly observed for groupers of this genus at aggregations. The sound has a short pulsing section followed by a longer tonal portion with a mean peak frequency below 100 Hz. Courtship-associated sounds were quantified over one spawning season at Mona Island, Puerto Rico. Most of the daily sound production occurred during a period of 2 h prior to sunset. The highest rates of sound production lasted for a period of 10 days with lunar periodicity over three consecutive months coincident with the reported season of reproduction. Passive acoustics provide a tool to measure the variability of the reproductive activity of M. bonaci over time and may provide a method to evaluate current strategies designed to protect multi-species spawning aggregations that are critical for the recovery of threatened groupers. 相似文献