An ecological perspective on nanomaterial impacts in the environment

Growing concerns over the potential for unintended, adverse consequences of engineered nanoparticles (ENPs) in the environment have generated new research initiatives focused on understanding the ecological effects of ENPs. Almost nothing is currently known about the fate and transport of ENPs in environmental waters, soils, and sediments or about the biological impacts of ENPs in natural environments, and the bulk of modern nanotoxicogical research is focused on highly controlled laboratory studies with single species in simple media. In this paper, we provide an ecological perspective on the current state of knowledge regarding the likely environmental impacts of nanomaterials and propose a strategy for making rapid progress in new research in ecological nanoscience.     

Oxidative enzymes in tissues of the catfish (<Emphasis Type="Italic">Clarias gariepinus</Emphasis>) exposed to varying levels of cadmium

The activities of some oxidative enzymes (aldehyde oxidase, AO, EC 1.2.3.1; xanthine oxidase, XO, EC 1.17.3.2; sulphite oxidase, SO, EC 1.8.3.1; and monoamine oxidase, MO, EC 1.4.3.4) were investigated in the liver, gill, kidney, brain and muscle of a variety of African catfish (Clarias gariepinus) after 7 and 21 days of exposure to 0.1, 0.2 and 0.4 ppm cadmium (Cd) in ambient water. MO, SO and AO activities were significantly (p < 0.05) decreased in a dose-dependent manner in the liver, kidney and gill in the 7-day Cd-exposed fish when compared to control. Conversely, brain MO, AO and SO activities were significantly (p < 0.05) elevated in a dose-dependent manner in the same fishes relative to control. As in the 7-day Cd-exposed fish, the activities of MO, AO and SO were significantly (p < 0.05) decreased in a dose-dependent manner in the gill, kidney and liver of catfish exposed for 21 days. Same is true for XO in these organs after both duration of exposure, except in the liver where no significant change was observed relative to control. The inhibition of these oxidative enzymes in the liver, gill and kidney of catfish may interfere with their biotransformation function, and ultimately the survival of the fish may be compromised.     

Gender,age and subject matter: impact on teachers’ ecological values

By means of environmental education, teachers are supposed to intervene in pupils’ awareness and concern of environmental issues. Teachers’ environmental attitudes, however, have rarely been studied. We analysed 367 German pre- and in-service teachers’ environmental attitudes for their structure and socio-economic correlates within the frame of a European project. Thus, we retrieved for adults the two-dimensional model of ecological values (2-MEV) presented for adolescents by Bogner and Wiseman (Sci Educ Int 15(1):27–48, 2004). For the two domains, Preservation and Utilisation, the psychometric structure was confirmed and discriminative correlations with age, gender and teaching subject were unveiled. Further research is needed to deduce the implications for teaching practice.     

Evaluation of the gene expression changes in Nile tilapia (<Emphasis Type="Italic">Oreochromis niloticus</Emphasis>) as affected by the bio-removal of toxic textile dyes from aqueous solution in small-scale bioreactor

A laboratory-scale bioremediation unit was designed, built and tested for the bio-removal of several Direct textile dyes. Four experiments were carried out to assess the efficiency of the bioremediation unit using Aspergillus niger fungal strain. Three commonly used Direct dyes and textile dyes mixture (simulated effluent: Direct brown, Direct violet, Direct green) were tested in this study. The strain of A. niger was efficient in the removal of the three Direct dyes. The decolorization percentages of the dyes after 24 h of incubation were 56.2, 51.7, and 95.4% for Direct brown, Direct green, Direct violet dyes, respectively. The percentages increased up to 79.4, 86.4, and 96.7% after 72 h of incubation for the same dyes, respectively. The results also showed that the fungal strain reduced the chemical oxygen demand values of simulated dye effluents from 165 to 564 mg/l with most of the dyes. The assessment of bioremediation products on biomodel was conducted using a fresh water fish. The liver and brain of Nile tilapia were tested to evaluate the expression of genes coding for several proteins related to stress such as metallothioneins (MTs), cytochrome P450 (CYP450), and heat shock proteins (HSPs). To assess the alterations in the gene expression, ten animals from each group were killed after 4 weeks of treatment. The results revealed significant increases in the brain and hepatic mRNA levels of all stress protein genes MT, CYP450, Hsp70a, b, and Hsp47 in the fish groups treated with industrial Direct violet, green, and brown dye water. Exposure of tilapia to bioremediation products after treatment with A. niger fungi reduced the over-expression of the stress protein genes in the brain and liver tissues.     

Annual nutrients budget for the grazed and ungrazed sites of an alpine expanse in North-West Himalaya,India

This work was undertaken to analyze nutrient contents of vegetation in an alpine meadow—Tungnath, North-West Himalaya, India. The study pertains to the uptake, transfer and release of four main macronutrients (organic carbon, total nitrogen, total potassium and total phosphorus) in grazed (exposed to extensive grazing by cattles) and ungrazed (grazing completely prohibited) communities. Mineral concentration was recorded higher for the ungrazed sites compared to the grazed sites, and maximum standing state of nutrients was found in roots. Belowground compartment (roots) contributed maximum share of mineral elements to soil. Litter nutrients release was low because of low microbial activity and continuous removal of phytomass. Observations reveal that there was very little amount of nutrient release from phytomass and vegetation in alpine are very poor source of mineral recycling. Low transfer rate of minerals from one compartment to other is adequate for greater amount of these minerals that are translocated back into the storage organs. A small proportion get removed through rain splash or through the removal of hay during grazing as relatively high release rates in ungrazed sites when compared to grazed sites was observed. This translocation can be considered as an important adaptation in alpine plants for survival during adverse environmental conditions, against all types of biotic pressures and also for regeneration in the forthcoming growing season.     

Building the role of local government authorities towards the achievement of the human right to water in rural Tanzania

In recent decades, many changes have occurred in the approach to financing and operating water services in developing countries. The demand‐responsive approach is now adopted in many countries in a context of donor‐supported decentralization processes, which gives more responsibility to end users. However, the government's responsibility at different levels is enforced by the international recognition of the human right to water. This paper examines specific actions that build the role of local government authorities in this scenario. A collaboration between an international NGO and a rural district in Tanzania from 2006 to 2009 is used as an action research case study that is representative of local capacity‐building needs in decentralized contexts and rural areas. Three main challenges were detected: i) lack of reliable information; ii) poor allocation of resources in terms of equity; and iii) lack of long‐term community management support from the district. Two mechanisms were established: i) water point mapping as a tool for information and planning; and ii) a District Water and Sanitation Unit Support (DWUS) for community management. The results show how the framework provided by the goal of human right to water helps to define useful strategies for equity‐oriented planning and post‐project support at the local level.     

Belowground effects of enhanced tropospheric ozone and drought in a beech/spruce forest (Fagus sylvatica L./Picea abies [L.] Karst)

The effects of experimentally elevated O₃ on soil respiration rates, standing fine-root biomass, fine-root production and δ¹³C signature of newly produced fine roots were investigated in an adult European beech/Norway spruce forest in Germany during two subsequent years with contrasting rainfall patterns. During humid 2002, soil respiration rate was enhanced under elevated O₃ under beech and spruce, and was related to O₃-stimulated fine-root production only in beech. During dry 2003, the stimulating effect of O₃ on soil respiration rate vanished under spruce, which was correlated with decreased fine-root production in spruce under drought, irrespective of the O₃ regime. δ¹³C signature of newly formed fine-roots was consistent with the differing g_s of beech and spruce, and indicated stomatal limitation by O₃ in beech and by drought in spruce. Our study showed that drought can override the stimulating O₃ effects on fine-root dynamics and soil respiration in mature beech and spruce forests.