Prenatal diagnosis of GM2-gangliosidosis B1 variant

Prenatal diagnosis in a pregnancy at risk for a juvenile B1 variant of GM₂-gangliosidosis was carried out. The biochemical study of the cultured amniocytes and the affected fetal brain is reported. The results obtained show that the sulphated artificial substrate can be used in the diagnosis of B1 variant, but not the neutral one. The accumulation of GM₂-ganglioside in the fetal brain of the B1 juvenile form and an infantile form of GM₂-gangliosidosis (0 variant) was compared.     

Optimisation of a PMAxx™-RT-qPCR Assay and the Preceding Extraction Method to Selectively Detect Infectious Murine Norovirus Particles in Mussels

Human noroviruses are a major cause for gastroenteritis outbreaks. Filter-feeding bivalve molluscs, which accumulate noroviruses in their digestive tissues, are a typical vector for human infection. RT-qPCR, the established method for human norovirus detection in food, does not allow discrimination between infectious and non-infectious viruses and can overestimate potentially infectious viral loads. To develop a more accurate method of infectious norovirus load estimation, we combined intercalating agent propidium monoazide (PMAxx™)-pre-treatment with RT-qPCR assay using in vitro-cultivable murine norovirus. Three primer sets targeting different genome regions and diverse amplicon sizes were used to compare one-step amplification of a short genome fragment to three two-step long-range RT-qPCRs (7 kbp, 3.6 kbp and 2.3 kbp amplicons). Following initial assays performed on untreated infectious, heat-, or ultraviolet-inactivated murine noroviruses in PBS suspension, PMAxx™ RT-qPCRs were implemented to detect murine noroviruses subsequent to their extraction from mussel digestive tissues; virus extraction via anionic polymer-coated magnetic beads was compared with the proteinase K-dependent ISO norm. The long-range RT-qPCR process detecting fragments of more than 2.3 kbp allowed accurate estimation of the infectivity of UV-damaged murine noroviruses. While proteinase K extraction limited later estimation of PMAxx™ pre-treatment effects and was found to be unsuited to the assay, magnetic bead-captured murine noroviruses retained their infectivity. Genome copies of heat-inactivated murine noroviruses differed by 2.3 log₁₀ between RT-qPCR and PMAxx™-RT-qPCR analysis in bivalve molluscs, the PMAxx™ pre-treatment allowing a closer approximation of infectious titres. The combination of bead-based virus extraction and PMAxx™ RT-qPCR thus provides a more accurate model for the estimation of noroviral bivalve mollusc contamination than the conjunction of proteinase K extraction and RT-qPCR and has the potential (once validated utilising infectious human norovirus) to provide an added measure of security to food safety authorities in the hazard assessment of potential bivalve mollusc contamination.

     

Application of CGP-cross route process for microstructure refinement and mechanical properties improvement in steel sheets

A modified method of severe plastic deformation (SPD) entitled constrained groove pressing-cross route (CGP-CR) was introduced for imposing a high magnitude of equivalent strain of about 2.32 per pass on the sheet form samples. The major benefit of this improved route compared to previous common route was the more homogeneity of strain in the rolling (RD) and transverse (TD) directions of sheets. In this study, low carbon steel samples were used for examination of evolutions in microstructure and mechanical properties during SPD via CGP-CR process. Mechanical properties improvement were measured by tensile and macro hardness tests. The results indicate that CGP-CR process can effectively improve tensile strength; and also, yield stress and hardness of as-received low carbon steel samples were improved up to about 100% after two deformation passes. Also, high magnitude of inhomogeneity can be observed in hardness distribution through first pass of the process which diminishes in the subsequent passes. Microstructural evolutions during process were monitored by optical microscopy observations and X-ray diffraction analysis. The results demonstrate that initial ferritic microstructure with grain size of about 30 μm was refined to a 225 nm cell structure after two passes of CGP-CR process.     

Prevalence of Norovirus Infection in Children and Adults with Acute Gastroenteritis,Tehran, Iran, 2008–2009

Noroviruses are one of important agents that cause acute viral gastroenteritis worldwide. These viruses are belonging to Caliciviridae family and are genetically diverse. To date, there is no valuable data about prevalence of norovirus infection and the dominant genogroup/genotype among Iranian population. The objective of this study was to determine the frequency of norovirus infection in Iranian patients with gastroenteritis referred to three hospitals of Tehran and to specify the dominant genogroup/genotype of this virus among our study population. A total of 293 patients with acute gastroenteritis were included in the study. Detection of norovirus was performed using RT-PCR method and confirmed by direct sequencing with specific designed primers for capsid region of norovirus genome. Phylogenetic analysis was performed using the neighbor-joining method. Norovirus strains identified in our study were subsequently categorized according to previously defined genogroup/genotypes. Of these, norovirus GII was dominant genogroup. Sixty-five percent (17 of 26) of positive samples were determined as GII and 35% (9 of 26) were determined as GI, respectively, in 2008–2009. And among 8 sequenced strains of genogroup II the most frequent genotype was GII.3. The results of this study indicated that norovirus must be considered as one of the infectious causes of acute gastroenteritis among Iranian population. We also found that GII.3 is more prevalent in our study population. To the best of our knowledge there is limited data about the role of noroviruses in children and adults’ acute gastroenteritis among Iranian patients and this prevalence and genotyping report of norovirus infection could be remarkable for further studies.     

Life cycle assessment of lithium-ion batteries for plug-in hybrid electric vehicles – Critical issues

The main aim of the study was to explore how LCA can be used to optimize the design of lithium-ion batteries for plug-in hybrid electric vehicles. Two lithium-ion batteries, both based on lithium iron phosphate, but using different solvents during cell manufacturing, were studied by means of life cycle assessment, LCA. The general conclusions are limited to results showing robustness against variation in critical data. The study showed that it is environmentally preferable to use water as a solvent instead of N-methyl-2-pyrrolidone, NMP, in the slurry for casting the cathode and anode of lithium-ion batteries. Recent years’ improvements in battery technology, especially related to cycle life, have decreased production phase environmental impacts almost to the level of use phase impacts. In the use phase, environmental impacts related to internal battery efficiency are two to six times larger than the impact from losses due to battery weight in plug-in hybrid electric vehicles, assuming 90% internal battery efficiency. Thus, internal battery efficiency is a very important parameter; at least as important as battery weight. Areas, in which data is missing or inadequate and the environmental impact is or may be significant, include: production of binders, production of lithium salts, cell manufacturing and assembly, the relationship between weight of vehicle and vehicle energy consumption, information about internal battery efficiency and recycling of lithium-ion batteries based on lithium iron phosphate.     

Baselines for land-use change in the tropics: application to avoided deforestation projects

Although forest conservation activities, particularly in the tropics, offer significant potential for mitigating carbon (C) emissions, these types of activities have faced obstacles in the policy arena caused by the difficulty in determining key elements of the project cycle, particularly the baseline. A baseline for forest conservation has two main components: the projected land-use change and the corresponding carbon stocks in applicable pools in vegetation and soil, with land-use change being the most difficult to address analytically. In this paper we focus on developing and comparing three models, ranging from relatively simple extrapolations of past trends in land use based on simple drivers such as population growth to more complex extrapolations of past trends using spatially explicit models of land-use change driven by biophysical and socioeconomic factors. The three models used for making baseline projections of tropical deforestation at the regional scale are: the Forest Area Change (FAC) model, the Land Use and Carbon Sequestration (LUCS) model, and the Geographical Modeling (GEOMOD) model. The models were used to project deforestation in six tropical regions that featured different ecological and socioeconomic conditions, population dynamics, and uses of the land: (1) northern Belize; (2) Santa Cruz State, Bolivia; (3) Paraná State, Brazil; (4) Campeche, Mexico; (5) Chiapas, Mexico; and (6) Michoacán, Mexico. A comparison of all model outputs across all six regions shows that each model produced quite different deforestation baselines. In general, the simplest FAC model, applied at the national administrative-unit scale, projected the highest amount of forest loss (four out of six regions) and the LUCS model the least amount of loss (four out of five regions). Based on simulations of GEOMOD, we found that readily observable physical and biological factors as well as distance to areas of past disturbance were each about twice as important as either sociological/demographic or economic/infrastructure factors (less observable) in explaining empirical land-use patterns. We propose from the lessons learned, a methodology comprised of three main steps and six tasks can be used to begin developing credible baselines. We also propose that the baselines be projected over a 10-year period because, although projections beyond 10 years are feasible, they are likely to be unrealistic for policy purposes. In the first step, an historic land-use change and deforestation estimate is made by determining the analytic domain (size of the region relative to the size of proposed project), obtaining historic data, analyzing candidate baseline drivers, and identifying three to four major drivers. In the second step, a baseline of where deforestation is likely to occur–a potential land-use change (PLUC) map—is produced using a spatial model such as GEOMOD that uses the key drivers from step one. Then rates of deforestation are projected over a 10-year baseline period based on one of the three models. Using the PLUC maps, projected rates of deforestation, and carbon stock estimates, baseline projections are developed that can be used for project GHG accounting and crediting purposes: The final step proposes that, at agreed interval (e.g., about 10 years), the baseline assumptions about baseline drivers be re-assessed. This step reviews the viability of the 10-year baseline in light of changes in one or more key baseline drivers (e.g., new roads, new communities, new protected area, etc.). The potential land-use change map and estimates of rates of deforestation could be re-done at the agreed interval, allowing the deforestation rates and changes in spatial drivers to be incorporated into a defense of the existing baseline, or the derivation of a new baseline projection.     

Prenatal cannibalism in an insect

Host selection and infection strategies of parasitoids often correlate with high parental investment and low numbers of progeny. In this study, we investigate how additional internal mechanisms might shape brood size and fitness of the offspring. Emblemasoma auditrix is a parasitoid fly in which about 38 larvae hatch simultaneously in utero. After host location, a single larva is deposited into the host, where it rapidly develops and pupates after about 5 days. The search for hosts can take several weeks, and during that time, the larvae arrest their development and remain in the first larval instar. Nevertheless, the larvae increase in weight within the uterus, and this growth correlates to a decrease in the number of larvae, although no larvae are deposited. Thus, our data indicate a first case of prenatal cannibalism in an invertebrate with larvae feeding on each other within the uterus of the adult.     

A juvenile lizard specimen with well-preserved skin impressions from the Upper Jurassic/Lower Cretaceous of Daohugou, Inner Mongolia, China

Lizards are now relatively well known from the Yixian Formation of northeastern China. In this study, we describe a juvenile lizard from a fossil horizon at Daohugou, Inner Mongolia. These beds predate the Yixian Formation, and are probably Late Jurassic or earliest Cretaceous in age. The new specimen thus documents the first lizard material from the Daohugou locality and is the earliest lizard skeleton from China. Comparisons with developmental stages of modern lizards suggest the Daohugou lizard is a hatchling. Although tiny, the specimen is notable in preserving exquisite skin impressions showing the variation in scalation across the body, the shape and position of the cloacal outlet, and details of the manus and pes. These are the earliest recorded lepidosaurian skin traces. In its general proportions and the possession of paired frontals, the small Daohugou lizard resembles both the Yixian taxon Yabeinosaurus tenuis and the questionable Jeholacerta formosa, but it differs from the latter in scalation and, based on other characters, may be distinct from both.     

Male satin bowerbirds (<Emphasis Type="Italic">Ptilonorhynchus violaceus</Emphasis>) compensate for sexual signal loss by enhancing multiple display features

Numerous studies have focussed on the relationship between female choice and the multiple exaggerated sexual traits of males. However, little is known about the ability of males to actively enhance specific components of their display in response to the loss of one component. We investigated the capacity of male satin bowerbirds (Ptilonorhynchus violaceus) to respond to the loss of one of their sexual signals by performing an experiment in which we removed decorations at their bowers. We found that males compensated for decoration loss by increasing bower construction behaviour and decreasing their latency to bower painting. These results are novel because they suggest that males can assess the quality of their own display and make decisions about how to augment their displays. We discuss these results in the context of previous studies of mate choice in satin bowerbirds, as both of the supplementary behaviours we observed are known correlates of male mating success.