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201.
Development of an enzyme-linked immunosorbent assay for atrazine monitoring in water samples 总被引:1,自引:0,他引:1
Diana L. D. Lima Rudolf J. Schneider Valdemar I. Esteves 《Environmental science and pollution research international》2013,20(5):3157-3164
The implementation of the Water Framework Directive (2000/60/EC) requires the establishment of monitoring programs. However, conventional procedures for sample preparation prior to chromatographic analysis are rather expensive and time consuming, being the development of cost-effective and easy tool a necessity. The aim of this work was to develop an enzyme-linked immunosorbent assay (ELISA) able to determine atrazine in water samples. Matrix effects evaluation showed that the increase of humic acid (HA) concentration leads to flattened calibration curves and to the loss of the sigmoidal shape. However, such interference was overcome, by the presence of an environmental sample buffer, incubated together with the samples. Recoveries from 88.5 to 119.2 % were obtained in the presence of HA concentrations up to 20 mg?L?1. An analytical range from 0.003 to 1 μg?L?1 was obtained, and atrazine was detected in a sewage treatment plant with concentrations ranging from 14 to 52 ng?L?1. 相似文献
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Schneider Silvana Demarqui Fábio de Freitas Costa Eduardo 《Environmental and Ecological Statistics》2022,29(4):869-911
Environmental and Ecological Statistics - Populations of free-ranging dogs are still a matter of concern in developing countries. The presence of stray dogs is associated with environmental and... 相似文献
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Neil M. Ram Mark W. Schneider Chase A. Gerbig Nancy Nevins Adam H. Love 《补救:环境净化治理成本、技术与工艺杂志》2019,30(1):33-45
Allocating costs for hazardous waste site cleanup among potentially responsible parties is unique to the specific circumstances, history, and data for the site. As such, distinct technical approaches are often needed considering the Gore and Torres factors to develop an overall allocation. These factors include both technical and nontechnical considerations that the trier of fact must weigh to arrive at the overall allocation. This article reviews the legal and technical framework in allocating cleanup costs at hazardous waste sites and presents several case histories illustrating the integration of fact, science and law in the final allocation. 相似文献
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Schneider A 《Journal of environmental quality》2006,35(1):394-401
The understanding of the availability of a metal in soil necessitates a minimum knowledge about its speciation in the soil solution. Here, we evaluated an alternative to the use of ion exchangers for estimating the free ionic fraction of cadmium (FCd) in solution. It is based on the exchange selectivity coefficient (VK) rather than the distribution coefficient (DK) to estimate FCd. Because VK for the Cd-Ca exchange for the used Amberlite resin was independent of the solution Ca concentration (0.5-7.5 mM) and pH (range: 4.5-6), the experiment on a solution mimicking the analyzed solution to estimate VK was not necessary. The influence of variable Ca and Mg concentrations in solution on FCd was assessed in synthetic solutions containing either citrate or malate. The best way to estimate FCd seemed to treat the exchange data as if Ca was solely present. However, neither the proposed approach nor those applying DK prevent the overestimation of FCd when Ca is partly complexed in the analyzed solution. A method intending to estimate two replicates of FCd for a given, unique solution was also studied on solutions issued from sorption-desorption experiments performed on a humic podzol. It consists of two successive supplies of a known resin mass to a unique sample. Both estimates were close and not significantly different. 相似文献
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