中瑞排污许可证制度的对比研究

对中国水排污许可和瑞典综合排污许可的审批程序和要求、审批内容等进行了对比分析。重点分析了环境法庭在瑞典综合排污许可申报过程中的作用和功能,以及瑞典排污许可证文件的起草和颁发过程。在此基础上,从政府、企业和公众3个层面提出了瑞典综合排污许可证制度对中国排污许可证制度建设和完善的重要借鉴意义。     

餐厨垃圾高温厌氧消化产沼气的试验研究

探究了温度、含固率、pH及接种率对餐厨垃圾厌氧消化产沼量的影响,并设计L9(34)正交试验进行厌氧发酵工艺优化研究。结果表明,在温度为55℃、含固率为8%(质量分数)、pH为7.00、接种率为90%(质量分数)时,厌氧发酵累积产沼量为809.0mL/g(以挥发性固体计);各影响因素对产沼量的影响大小为温度pH接种率含固率。     

Dioxin-like activity in sediments from Tai Lake, China determined by use of the H4IIE-luc bioassay and quantification of individual AhR agonists

Deterioration of the general ecosystem and specifically quality of the water in Tai Lake (Ch: Taihu), the third largest freshwater in China, is of great concern. However, knowledge on status and trends of dioxin-like compounds in Tai Lake was limited. This study investigated AhR-mediated potency and quantified potential aryl hydrocarbon receptor (AhR) agonists in sediments from four regions (Meiliang Bay, Zhushan Lake, Lake Center, Corner of Zhushan Lake, and Meiliang Bay) of Tai Lake by use of the in vitro H4IIE-luc, cell-based, transactivation, reporter gene assay, and instrumental analysis. Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (Bio-TEQs) in sediments ranged from less than the limit of detection to 114.5 pg/g, dry weight, which indicated that organic extracts of sediments exhibited significant AhR-mediated potencies. Results of the potency balance analysis demonstrated that acid-labile, dioxin-like compounds represented a greater proportion of concentrations of Bio-TEQs in sediments from Tai Lake. Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents calculated as the sum of the product of concentrations of individual congeners and their respective relative potencies (Chem-TEQs) based on polycyclic aromatic hydrocarbons and/or polychlorinated biphenyls represented no more than 10 % of the total concentrations of Bio-TEQs.     

Characterization of the interactions between tetracycline antibiotics and microbial extracellular polymeric substances with spectroscopic approaches

The antibiotics have attracted global attentions for their impact on aquatic ecosystem. The knowledge about the fate of antibiotics encountering extracellular polymeric substances (EPS) is, however, limited. In this study, we investigated the interacting mechanisms of tetracycline (TC) to EPS extracted from aerobic activated sludge. The contributions of the main components of EPS, extracellular proteins, and polysaccharides were evaluated using bovine serum albumin and alginate sodium, respectively. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and nuclear magnetic resonance indicated that hydroxyl, carboxyl, and amino groups were the domain chemical groups involved in the interaction between TC and EPS, and the binding of TC onto EPS changed the structure of these chemical groups, thus causing shifts in their UV–visible absorption spectra. In addition, we found that extracellular proteins, rather than polysaccharides, were the major active contents involved in the interaction. Three-dimensional excitation–emission matrix fluorescence spectroscopy showed that the fluorophores in EPS were clearly quenched by TC and the static quenching process was observed, implying the complex formation of TC and EPS. Furthermore, thermodynamic analysis indicated that the binding of TC with EPS is spontaneous and dominated by electrostatic forces.     

Medium optimization for the production of anti-cyanobacterial substances by Streptomyces sp. HJC-D1 using response surface methodology

Bioremediation using isolated anti-cyanobacterial microorganism has been widely applied in harmful algal blooms (HABs) control. In order to improve the secretion of activated anti-cyanobacterial substances, and lower the cost, a sequential optimization of the culture medium based on statistical design was employed for enhancing the anti-cyanobacterial substances production and chlorophyll a (Chl a) removal by Streptomyces sp. HJC-D1 in the paper. Sucrose and KNO₃ were selected as the most suitable carbon and nitrogen sources based on the one-at-a-time strategy method, and sucrose, KNO₃ and initial pH were found as major factors that affected the anti-cyanobacterial ability of the isolated stain via the Plackett–Burman design. Based on the response surface and canonical analysis, the optimum condition of culture medium was obtained at 22.73 g l^-1 of sucrose, 0.96 g l^-1 of KNO₃, and initial pH 8.82, and the Chl a removal efficiency by strain HJC-D1 increased from 63?±?2 % to 78?±?2 % on the optimum conditions.     

Distribution and ecological risk assessment of organochlorine pesticides in surface sediments from the East Lake,China

Organochlorine pesticides (OCPs) are ubiquitous pollutants, and their presence in urban lakes is a concern for human and ecological health. Surface sediments in the East Lake, China, were collected in winter 2012 and summer 2013 to investigate concentrations, distribution patterns, possible sources, and potential ecological risks of OCPs in this area. The total concentrations of 14 OCPs ranged from 6.3 to 400 ng g^?1 dry weight (dw) with an average concentration of 79 ng g^?1 dw. The mean values of hexachlorocyclohexanes (HCHs) (α-, β-, γ-, and δ-HCH) and dichlorodiphenyltrichloroethanes (DDTs) (p,p’-DDE, p,p’-DDD, and p,p’-DDT) were 36 and 7.6 ng g^?1 dw, accounting for 45 and 10 % of the total OCPs, respectively. The concentrations of OCPs in sediment samples collected in winter were significantly higher than those in summer, especially the HCHs, of which in winter were two times greater than summer. Composition analyses indicated that DDTs and endosulfan were mainly from historical contribution. Historical use of technical HCH and new input of lindane were probably the source of HCHs in the East Lake. Most sampling sites of HCHs and DDTs were found to have the potential ecological risk based on levels specified in the sediment quality standards.     

循环流化床锅炉燃煤技术热电厂生命周期评价

运用生命周期评价方法,应用Gabi5.0软件对采用循环流化床锅炉燃煤技术的热电企业进行资源耗竭和环境影响分析,计算各生产单元生命周期清单的全球变暖潜值、酸化效应潜值、富营养化潜值、非生物资源耗竭潜值、人体毒性潜值以及光化学烟雾和臭氧生成潜值等主要环境影响类型,对燃煤发电过程进行生命周期评价。结果表明,全球变暖和非生物资源耗竭为发电运行过程中主要的环境影响因素,分别占53%和15.05%;锅炉燃烧阶段的环境影响最重,占整个生命周期影响值的77.12%,并且除生物耗竭潜值外的其他5项指标均为发电运行阶段各个单元中最高。采用SNCR脱硝技术减少燃烧过程中NOx的排放量,并对该技术方案进行生命周期评价,比较方案实施前后的环境影响。     

抗铬菌株的筛选鉴定及其生物学特性和吸附特性

利用微生物分离纯化技术,在培养基中加入不同浓度的Cr3+、Cr6+,经过长时间驯化,从污染土壤中筛选出2株对Cr具有较高抗性的菌株(A和B),对其进行了形态、生理生化特性及分子生物学鉴定,并研究了其最佳生长条件及对Cr3+的生物吸附规律。结果表明,菌株A和B分别为蜡状芽孢杆菌(Bacillus cereus)和米根霉菌(Rhizopus oryzae)。菌株A和B的最适生长pH值均为7,最适生长温度均为30℃,最佳装液量为80~100 mL(250 mL摇瓶)。在培养时间一定时,随着Cr3+浓度的增加,菌株对Cr3+的吸附率减少,而生物吸附量则逐渐增大;在Cr3+浓度一定时,随着培养时间的延长,菌体对Cr3+的吸附率呈现出先增加后减少的趋势,这与生物吸附量的变化趋势基本一致。在Cr3+浓度为100 mg/L时,菌株A和B对Cr3+的吸附率达到最大,分别为51.47%和46.36%。     

直接投菌法在城市重污染河流治理中的应用研究

在无截污和清除内源的情况下,对深圳市甘坑河采用梅花式接种法将本源微生物菌剂分两次直接接入河流水体及底泥中。结果表明,第1次接种后的4 d内各项水质指标都不稳定,4 d后,悬浮物、COD、TP和NH3-N等浓度下降,水质明显改善;但从第8天开始,COD、NH3-N和TP的去除率同时下降,本源微生物的处理效率开始降低,需进行第2次接种;第2次接种后,DO浓度提升至2.0 mg/L以上,COD、TP和NH3-N的去除率分别保持在40%、30%和40%以上。结果表明,直接投菌法在城市重污染河道治理中可以初步消除河道的黑臭现象,修复水质。     

Biodegradation of beta-cypermethrin by a novel Azoarcus indigens strain HZ5

A novel strain HZ5 was isolated from the activated sludge of a pesticide manufacturer in Hangzhou, which was capable of degrading beta-cypermethrin (beta-CP). Based on its physiological characteristics and analysis of 16S rDNA gene, strain HZ5 was identified as Azoarcus indigens, which was a new genus that can degrade beta-CP effectively. Strain HZ5 could degrade beta-CP over a wide range of temperature (20 to 40°C) and pH (5.5 to 9.0), and the optimal temperature and pH were 30°C and 7.0. The highest degradation rate was approximately 70% of 50 mg/L beta-CP within 144 h at pH 7.0 and 30°C in MSM. An additional carbon source could enhance the biodegradation of beta-CP. Studies on biodegradation of the beta-CP showed no significant enantioselectivity. During the process, two main intermediate metabolites were produced by strain HZ5 and determined as 3-phenoxybenzaldehyde and 3-phenoxybenzoic acid by gas chromatography-mass spectrometry (GC-MS) analysis. The results indicated that strain HZ5 may have potential application in bioremediation of beta-CP polluted environment.