In this research, epigenetic effects of bisphenol A (BPA) on human breast cancer MCF-7 cells were analyzed. Genome-wide DNA methylation and gene expression were analyzed in MCF-7 cells exposed to BPA (10?5 and 10?6 mol/L for 5 weeks). No significant changes in the global level of 5-methyl-2′-deoxycytidine and 5-hydroxymethyl-2′-deoxycytidine were observed. DNA methylation profiling analysis indicated that BPA exposure resulted in the hypermethylation of FOXK2, LKB1, LMX1A and CUGBP2 and the hypomethylation of PTPRN2, TRIM27, BCAS3 and ZNF423. Decreased expression of apoptosis genes (P38 and BCL2L1) and increased expression of chemokine (Cxcl2 and ccl20) were detected. Changes of these genes were speculated to affect the ERα-related cell growth as well as cell apoptosis. 相似文献
Zinc is known as an essential element of human life. However, excessive zinc discharge into water and soil causes water pollution, leading to serious health issues such as septicemia, meningitis and iron-deficiency anemia. Here, a novel material made of struvite-supported diatomite was obtained from eutrophic water treated by mesoporous MgO-modified diatomite. This material was applied for zinc remediation in aqueous solutions and contaminated soils to test the reuse of P-containing products. Struvite-supported diatomite was characterized by field emission scanning electron microscopy and X-ray diffraction. Results show that the maximum removal efficiency of Zn(II) from wastewater streams reached 90.54% at an initial pH of 5 and struvite-supported diatomite dosage of 0.3 g/L. Moreover, the X-ray diffraction patterns of precipitates after Zn(II) sorption show that the combination between zinc and the phosphate group played a key role for zinc removal in solution. For Zn-contaminated soils amended with 10% struvite-supported diatomite, available Zn decreased by 65.38% and acid soluble Zn decreased by 56.9% after 56 days. 相似文献
Climate change has become increasingly serious due to the greenhouse effect. It is therefore necessary to control the content of greenhouse gases such as carbon dioxide in the atmosphere, using, for instance, CO2-adsorbing materials. Here, we synthesized ultra-lightweight and spherical cellulose nanofibres aerogels by a suspension titration method using an efficient amination process. These functional materials with high porosity, higher than 96.54%, and three-dimensional network structure, were prepared by freeze-drying spherical cellulose nanofibres hydrogel. Their maximum CO2 adsorption capacity reaches 1.78 mmol/g, and they show excellent regeneration, of more than 10 cycles. This synthesis of bioaerogels represents a new method for the preparation of bio-CO2 adsorbents. 相似文献
Channel confluences at which two channels merge have an important effect on momentum exchange and contaminant diffusion in both natural rivers and artificial canals. In this study, a three-dimensional numerical model, which is based on the Reynolds Averaged Navier–Stokes equations and Reynolds Stress Turbulence model, is applied to simulate and compare flow patterns and contaminant transport processes for different bed morphologies. The results clearly show that the distribution of contaminant concentrations is mainly controlled by the shear layer and two counter-rotating helical cells, which in turn are affected by the discharge ratio and the bed morphology. As the discharge ratio increases, the shear flow moves to the outer bank and the counter-clockwise tributary helical cell caused by flow deflection is enlarged, leading the mixing happens near the outer bank and the mixing layer distorted. The bed morphology can induce shrinkage of the separation zone and increase of the clockwise main channel helical cell, which is initiated by the interaction between the tributary helical cell and the main channel flow and strengthened by the deep scour hole. The bed morphology can also affect the distortion direction of the mixing layer. Both a large discharge ratio and the bed morphology could lead to an increase in mixing intensity. 相似文献
Identifying source information after river chemical spill occurrences is critical for emergency responses. However, the inverse uncertainty characteristics of this kind of pollution source inversion problem have not yet been clearly elucidated. To fill this gap, stochastic analysis approaches, including a regional sensitivity analysis method, identifiability plot and perturbation methods, were employed to conduct an empirical investigation on generic inverse uncertainty characteristics under a well-accepted uncertainty analysis framework. Case studies based on field tracer experiments and synthetic numerical tracer experiments revealed several new rules. For example, the release load can be most easily inverted, and the source location is responsible for the largest uncertainty among the source parameters. The diffusion and convection processes are more sensitive than the dilution and pollutant attenuation processes to the optimization of objective functions in terms of structural uncertainty. The differences among the different objective functions are smaller for instantaneous release than for continuous release cases. Small monitoring errors affect the inversion results only slightly, which can be ignored in practice. Interestingly, the estimated values of the release location and time negatively deviate from the real values, and the extent is positively correlated with the relative size of the mixing zone to the objective river reach. These new findings improve decision making in emergency responses to sudden water pollution and guide the monitoring network design.