Determination of pesticides of different chemical classes in drinking water of the state of Santa Catarina (Brazil) using solid-phase microextraction coupled to chromatographic determinations

The evaluation of the concentration of pesticides in drinking water presents a real concern. In this study, a simple and rapid method based on solid-phase microextraction (SPME) followed by gas chromatography–mass spectrometry and electron capture detectors was developed aiming at multiclass determination of 23 pesticides regulated by the Brazilian legislation. The extraction was carried out by direct immersion mode (DI-SPME) using DVB/Car/PDMS fiber coating. In order to improve the extraction efficiency, parameters such as temperature, salting-out effect, and extraction time were optimized. The method was evaluated using drinking water samples spiked with the analytes at different concentrations, and it showed good linearity in the range studied. The values obtained for limits of quantification (LOQ) were below the limits established by Brazilian regulations. Accuracy and precision of the method exhibited satisfactory results, providing relative recoveries from 70 to 123.34% at three spiked levels, and the relative standard deviations ranged from 0.53 to 24.8%. The method was applied in 20 drinking water samples from 13 cities in the State of Santa Catarina, Brazil.

     

Assessment of the hunting of mammals using local ecological knowledge: an example from the Brazilian semiarid region

The hunting of wild animals remains a common activity in various parts of the world, especially in rural communities with poor socioeconomic conditions. To investigate patterns of mammal hunting, this study adopted a rural community located in semiarid Brazil as a model to test whether variables such as perceived abundance, activity period (day or night) and animal biomass could influence the consumption potential of mammal taxa. For this study, a checklist/interview technique using 32 photographs of mammals recorded in the region and two photographs of species that did not occur in the region, which acted as “control” species, was used, in addition to other visual stimuli and free lists. All species presented in the photographs, with the exception of the “control” species, were recognized as occurring in the region. Fourteen species were cited as being those most hunted locally. The species cited as most hunted had an average perceived abundance higher than the group of the least-hunted species. However, there was no significant relationship between hunting of a species and its locally perceived abundance. No significant difference in hunting pressure between diurnal and nocturnal species was found, nor was a relationship between animal biomass and hunting pressure observed. Our findings suggest that perceived abundance is an important factor for choosing a resource fauna, but other factors such as intended use, meat flavor and vulnerability to hunting, among others, may influence the potential use of a species.     

Can fish gill anomalies be used to assess water quality in freshwater Neotropical systems?

Gill anomalies in two fish species (Geophagus brasiliensis and Astyanax bimaculatus) were compared among three freshwater systems with different water quality: one eutrophic river, one eutrophic reservoir, and one oligotrophic reservoir. The raised hypotheses are that reservoirs with low water quality (eutrophic) have fish with more gills anomalies compared with reservoirs with high water quality (oligotrophic), and that the more stable environmental conditions of eutrophic rivers have fish with better healthy conditions than eutrophic reservoirs that have lesser stable environmental conditions. Gills of 36 adult individuals of G. brasiliensis and 23 of A. bimaculatus collected during the winter 2008 and winter 2009 were examined, and the proportions of occurrence of nine histological alterations were compared for the two species among the three systems using a binomial t test for independent samples. Histological changes in fish gills that are reversible and unspecific, such as epithelial lifting, interstitial edema, leukocyte infiltration, hyperplasia of the epithelial cells, lamellar fusion, and vasodilatation were common in both fish species in the three systems. However, lamellar aneurism, which is a more serious and often irreversible anomaly, and lamellar blood congestion occurred only in fish from the two reservoirs. Alternatively, necrosis occurred more in fish from the river. Fish gill anomalies in both species did not differ between the two reservoirs, despite having different water quality. We rejected the hypothesis that reservoirs with lower water quality have fish with more gill injuries compared with high water quality reservoirs. Moreover, the eutrophic river seems to affect differently the healthy condition of fish species, compared with the eutrophic reservoir.     

Dissolved inorganic nutrients and chlorophyll a in an estuary receiving sewage treatment plant effluents: Cachoeira River estuary (NE Brazil)

Sampling was conducted monthly during a transition period between the dry and rainy seasons in order to evaluate the effectiveness of a municipal sewage treatment plant (STP) in eutrophication control. STP effluent and fluvial input data were also estimated. In the dry period, high concentrations of nutrients, chlorophyll a (up to 360 μg?L^?1), and anoxia in bottom waters were observed in the upper portion of the estuary. Nitrate was scarce during the dry months, although high concentrations were observed at the river sources and the upper estuary. The N:P and Si:P molar ratios were usually below 16:1, and the Si:N ratio was higher than 1:1. The fluvial inputs were a greater source of nutrients to the estuary than the STP, but nutrient loading by these effluents were also important in contributing to the eutrophication of the upper estuarine zone, especially in the dry season when symptoms were more intense.     

Inputs of heavy metals due to agrochemical use in tobacco fields in Brazil’s Southern Region

Only a few studies have assessed the joint incorporation of heavy metals into agricultural systems based on the range of agrochemicals used on a specific agricultural crop. This study was conducted to assess the heavy metals input through application of the main agrochemicals used in Brazilian tobacco fields. A total of 56 samples of different batches of 5 fertilizers, 3 substrates, 8 insecticides, 3 fungicides, 2 herbicides, and 1 growth regulator commonly used in the cultivation of tobacco in Brazil’s Southern Region were collected from 3 warehouses located in the States of Rio Grande do Sul, Santa Catarina, and Paraná. The total As, Cd, Co, Cr, Cu, Hg, Fe, Mn, Ni, Pb, and Zn content of the samples was then determined and compared with the regulations of different countries and information found in the available literature. The fertilizers were identified as the primary source of heavy metals among the agrochemicals used. Application of pesticides directly to the shoots of tobacco plants contributed very little to the supply of heavy metals. The agrochemicals used in Brazilian tobacco fields provide lower inputs of the main heavy metals that are nonessential for plants than those registered in the international literature for the majority of crop fields in different regions of the world.     

Lead isotope ratios in lichen samples evaluated by ICP-ToF-MS to assess possible atmospheric pollution sources in Havana,Cuba

Epiphytic lichens, collected from 119 sampling sites grown over “Roistonea Royal Palm” trees, were used to assess the spatial distribution pattern of lead (Pb) and identify possible pollution sources in Havana (Cuba). Lead concentrations in lichens and topsoils were determined by flame atomic absorption spectrophotometry and inductively coupled plasma (ICP) atomic emission spectrometry, respectively, while Pb in crude oils and gasoline samples were measured by ICP-time of flight mass spectrometry (ICP-ToF-MS). Lead isotopic ratios measurements for lichens, soils, and crude oils were obtained by ICP-ToF-MS. We found that enrichment factors (EF) reflected a moderate contamination for 71% of the samples (EF > 10). The ²⁰⁶Pb/²⁰⁷Pb ratio values for lichens ranged from 1.17 to 1.20 and were a mixture of natural radiogenic and industrial activities (e.g., crude oils and fire plants). The low concentration of Pb found in gasoline (<7.0 μg L^?1) confirms the official statement that leaded gasoline is no longer used in Cuba.     

Effect of the Recycling and Annealing on the Mechanical and Fracture Properties of Poly(Lactic Acid)

The aim of this work is to evaluate the use of Poly(lactic acid) (PLA) industrial waste as a source of raw material for certain applications, as well as to understand the effects of the annealing on the fracture behavior of PLA. PLA waste has been simulated by an initial step of extrusion in a single screw extruder and pelletizing. Specimens of virgin and reprocessed PLA were obtained by injection molding. An annealing treatment capable of increasing the percentage of crystallinity (determined by differential scanning calorimetry) was also analyzed in reprocessed and non reprocessed specimens. The fracture behavior was studied at slow and high testing speed, applying the linear elastic fracture mechanics (LEFM) on single edge notched bend (SENB) specimens. This study revealed that the fracture toughness of the reprocessed PLA was basically the same that the virgin PLA and also that the increase in the crystalline fraction produced an improvement on the fracture toughness, at slow loading rate.     

Arsenic removal from water employing heterogeneous photocatalysis with TiO2 immobilized in PET bottles

Arsenic oxidation (As(III) to As(V)) and As(V) removal from water were assessed by using TiO₂ immobilized in PET (polyethylene terephthalate) bottles in the presence of natural sunlight and iron salts. The effect of many parameters was sequentially studied: TiO₂ concentration of the coating solution, Fe(II) concentration, pH, solar irradiation time; dissolved organic carbon concentration. The final conditions (TiO₂ concentration of the coating solution: 10%; Fe(II): 7.0 mg l⁻¹; solar exposure time: 120 min) were applied to natural water samples spiked with 500 μg l⁻¹ As(III) in order to verify the influence of natural water matrix. After treatment, As(III) and total As concentrations were lower than the limit of quantitation (2 μg l⁻¹) of the voltammetric method used, showing a removal over 99%, and giving evidence that As(III) was effectively oxidized to As(V). The results obtained demonstrated that TiO₂ can be easily immobilized on a PET surface in order to perform As(III) oxidation in water and that this TiO₂ immobilization, combined with coprecipitation of arsenic on Fe(III) hydroxides(oxides) could be an efficient way for inorganic arsenic removal from groundwaters.     

Artisanal fisheries of the Itaparica reservoir,São Francisco River,Brazil: socioeconomic profile,environmental dynamics,and management recommendations

Currently, artisanal fisheries in fresh water reservoirs have been losing ground to commercial fish farming and a great deal of fishery resources are being threatened by human interventions, such as reservoir management and increasing net-cage aquaculture. This study aims to understand the impacts of environmental changes on fishery resources and analyse the migratory fish breeding season as a missing link for fishery management in the semi-arid area of the São Francisco River Basin. Among the 870 fishermen operating in the Pernambuco part of the Itaparica Reservoir, 10% were interviewed and affirmed that fish stocks have been compromised due to the closed fishing period’s incoherence, exotic species insertion, and changes to the river flow caused by reservoir construction and operation. A significant correlation was observed between the Reproductive Activity Index (RAI) and precipitation (p?=?0.745) as well as between the RAI and the river’s flow (p?=??0.909). This shows how important it is to consider both the semi-arid climate setting and reservoir operation in determining the closed period which should safeguard fish reproduction. Monitoring the fish reproduction period and research on the reproductive biology of native fish species is needed in the São Francisco River Basin’s different stretches in order to guarantee valuable fish stocks and fisheries maintenance. Integrated action between the fish resource users, civil society, and federal bodies/agencies is essential in order to mitigate impacts and improve fish production systems, not only in Brazil, but in other regions experiencing a similar scenario.