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91.
Methylmercury genotoxicity: a novel effect in human cell lines of the central nervous system 总被引:1,自引:0,他引:1
Crespo-López ME Lima de Sá A Herculano AM Rodríguez Burbano R Martins do Nascimento JL 《Environment international》2007,33(2):141-146
Methylmercury is an important source of environmental contamination and the central nervous system (CNS) is one of the main target organs. Methylmercury genotoxicity was already demonstrated in peripherical tissues but was never detected in the brain. Thus, the objective of this work was to verify its genotoxic effect using brain cell lines. Glioblastoma (U373) and neuroblastoma (B103) human cell lines were exposed to methylmercury (0-10 microM). By measuring cellular viability, concentrations inducing <20% of cellular death (P<0.05) were selected: 1 and 0.1 microM. To detect micronuclei, 200,000 cells were treated with methylmercury for 24 h, and then incubated with cytochalasin B (2 microg/ml) for 72 h (U373) or 48 h (B103). The binucleation index, frequency of micronucleated cells, micronucleation index, metaphasic index and index of nucleoplasmic bridges were determined. Statistical analysis showed indices and percentages significantly higher (P<0.05) in methylmercury-treated cells. Each cell line was shown to be differently sensitive to each biomarker of genotoxic damage, which seems to indicate the existence of different mechanisms of toxicity. This work demonstrates, for the first time, MeHg ability to provoke genotoxicity in cells of brain origin with relatively low levels of exposure. 相似文献
92.
ris Flvia Sousa Gonalves Terezinha Maria Souza Leonardo Rogrio Vieira Filipi Calbaizer Marchi Adailton Pascoal Nascimento Davi Felipe Farias 《Environmental science and pollution research international》2020,27(10):10185-10204
The use of zebrafish (Danio rerio) has arisen as a promising biological platform for toxicity testing of pesticides such as herbicides, insecticides, and fungicides. Therefore, it is relevant to assess the use of zebrafish in models of exposure to investigate the diversity of pesticide-associated toxicity endpoints which have been reported. Thus, this review aimed to assess the recent literature on the use of zebrafish in pesticide toxicity studies to capture data on the types of pesticide used, classes of pesticides, and zebrafish life stages associated with toxicity endpoints and phenotypic observations. A total of 352 articles published between September 2012 and May 2019 were curated. The results show an increased trend in the use of zebrafish for testing the toxicity of pesticides, with a great diversity of pesticides (203) and chemical classes (58) with different applications (41) being used. Furthermore, experimental outcomes could be clustered in 13 toxicity endpoints, mainly developmental toxicity, oxidative stress, and neurotoxicity. Organophosphorus, pyrethroid, azole, and triazine were the most studied classes of pesticides and associated with various toxicity endpoints. Studies frequently opted for early life stages (embryos and larvae). Although there is an evident lack of standardization of nomenclatures and phenotypic alterations, the information gathered here highlights associations between (classes of) pesticides and endpoints, which can be used to relate mechanisms of action specific to certain classes of chemicals. 相似文献
93.
Metal mixtures in pregnant women and umbilical cord blood at urban populations—Rio de Janeiro,Brazil
de Figueiredo Nataly Damasceno Araújo Monica Seefelder Luiz Ronir R de Magalhaes Câmara Volney do Couto Jacob Silvana dos Santos Lisia Maria Gobbo Vicentini Santos Alves Asmus Carmen Ildes Rodrigues Froes 《Environmental science and pollution research international》2020,27(32):40210-40218
Environmental Science and Pollution Research - This study aims to assess interrelationships between serum lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg) concentrations in pregnant women in... 相似文献
94.
Erica Souto Abreu Lima Talita de Santana Matos Helena Saraiva Koenow Pinheiro Leonardo Durval Duarte Guimarães Daniel Vidal Pérez Nelson Moura Brasil do Amaral Sobrinho 《Environmental monitoring and assessment》2018,190(6):364
The increasing concern regarding the preservation of soil quality suggests the adoption of quality reference values as a first step in environmental monitoring actions. In this context, the objective of this study was to establish soil quality reference values for Al, Zn, Mn, Cd, Fe, Pb, Ni, Cr, and Cu and to correlate several soil properties with the occurrence of these elements in the hillslope region of Rio de Janeiro State. Sixty-two samples were collected at depths of 0–20 and 20–40 cm and analyzed using the USEPA method 3051A. After clustering analysis, the samples were separated into three groups. Classification functions were obtained based on the variables pH - Mg - Fe - Mn, i.e., the variables that were best related to heavy metal content. We determined to establish the quality reference values for the 75th percentile of the three groups. The relief forms of the area, which facilitate the transport of soil fine-particle fractions and accumulation in lowlands, were revealed to be one of most important factors in understanding the distribution of soil heavy metals. 相似文献
95.
Moreno T Querol X Alastuey A García do Santos S Fernández Patier R Artiñano B Gibbons W 《Journal of environmental monitoring : JEM》2006,8(10):1060-1068
Source apportionment study was performed, applying principal component analysis to the results of 221 chemical analyses of PM10 and PM2.5 samples collected daily from the industrial (but low traffic) Spanish town of Puertollano over a 14-month period during 2004-2005. Results reveal compositional variations attributable to different mixtures of natural and anthropogenic materials, mainly soil and rock dust (crustal), marine salt (only in PM10), petrochemical refinery emissions, and particles attributed to the combustion of local coal, which is unusually rich in Pb and Sb. During the study period there were 34 pollution episodes when PM10 exceeded 50 tg m(-3), mostly due to winter air temperature inversions, regional atmospheric stagnation, or African dust incursions (North African, NAF days: usually in summer). Whereas the crustal component during NAF episodes averaged 52% with a PM2.5/PM10 ratio of 0.54, this dropped to 29% and a PM2.5/PM10 of 0.67 during non-NAF days when anthropogenic materials predominated. Abnormally enhanced concentrations of pathfinder metallic trace elements provide additional evidence for source apportionment: thus aerosols with raised levels of Pb and Sb are associated with local coal combustion, Ni and V can be linked to petrochemical PM emissions, and Ti, Mn, Rb, and Ce are particularly characteristic of crustal dust incursions. 相似文献
96.
Krüger RL Dallago RM Filho Ido N Di Luccio M 《Environmental monitoring and assessment》2009,158(1-4):355-363
This work reports the screening and characterization of odor compounds in gaseous effluents generated during the production of poultry feather and viscera meal, the by-products of the poultry meat industry. Chemical analysis was carried out by solid phase microextraction in the headspace (HS-SPME) mode. Exhaust air of thermal processing of poultry feather and viscera were sampled online from a bench-scale digester, condensed, and collected in sampling flasks. Both volatile and semivolatile organic compounds present in the condensed gases were extracted under agitation at constant temperature. The extracts were analyzed with a gas chromatograph coupled to a mass spectrometric detector (GC/MSD). The identification of compounds was carried out by comparing the mass spectra obtained with those from the Wiley library and quantification was accomplished through authentic analytical standards. For the determination of the best extraction conditions and analysis, extraction fibers of different coatings and polarities were tested: divinylbenzene/carboxen/polydimethyilsiloxane (DVB/CAR/PDMS), carbowax/divinylbenzene (CW/DVB), and polydimethylsiloxane (PDMS) and chromatography columns of different polarities: DB-WAX (polar) and DB-5 (nonpolar). The best extraction conditions and analysis of the compounds of interest were obtained by the use of the SPME fiber with DVB/CAR/PDMS coating and analysis by GC/MSD with polar capillary column. Several carboxylic acids were identified, as well as mercaptans, amines, and aldehydes of great environmental importance. 相似文献
97.
98.
V. T. Nascimento L. G. Sousa A. G. C. Alves E. L. Araújo U. P. Albuquerque 《Environment, Development and Sustainability》2009,11(5):1005-1029
Fences are very common in rural areas, and represent important landscape elements in both tropical and temperate climate regions.
In spite of their marked presence and importance, fences have been little studied, principally in Brazil. The present study
examined the types of fences, the diversity of species used in their construction, as well as the diversity of their uses
in a rural community in the municipality of Caruaru, Pernambuco State, northeastern Brazil. Fifty meters of fence-line in
each of 50 rural properties in the community were examined, noting the diameter, height and identity of all vegetation elements
used in their composition. Semi-structured interviews were also held with their builders in order to obtain information concerning
fence uses. In the total of 2,500 linear meters of fence-line, 4,953 individual plant elements were identified, belonging
to 51 different species. A majority of the fence elements were non-living fence posts, although the number of living posts
was high. Of the total number of fence elements, 66.7% were native to the caatinga region. The large number of native species used as non-living fence posts indicates an intensive use of the caatinga vegetation and suggests the need to stimulate the use of living fence posts for conservation purposes. 相似文献
99.
100.
Seixas TG Kehrig Hdo A Costa M Fillmann G Di Beneditto AP Secchi ER Souza CM Malm O Moreira I 《Environmental pollution (Barking, Essex : 1987)》2008,154(1):98-106
Selenium and total and organic mercury were determined in the liver and kidney of franciscana dolphin (Pontoporia blainvillei) incidentally caught in fishing nets along two Brazilian coastal areas (southeast and south). Regional differences in the concentrations of these contaminants were observed in P. blainvillei. Liver showed the highest organic and total mercury. In general, samples of individuals collected at the southern of Brazil had the highest concentrations of selenium and total and organic mercury. No significant gender differences were observed. Growth stage influenced the accumulation of these contaminants in both organs, and hepatic concentrations increased with the body length, according to the sampling area. Molar mercury and selenium concentrations in liver were significantly correlated, with a Se:Hg ratio close to 4. The among-site differences we found may be related to differences in preferred prey, bioavailability in the marine environment, environmental conditions, or these individuals may belong to distinct populations. 相似文献