Change in the antimicrobial resistance profile of Pseudomonas aeruginosa from soil after exposure to herbicides

The extensive use of pesticides represents a risk to human health and to the environment. This study aimed to investigate if the exposure to atrazine and diuron, two herbicides widely used in Brazil, could induce changes in the susceptibility profile to aztreonam, colistin and polymyxin B antimicrobials in isolates of P. aeruginosa obtained from soil samples by using the determination of minimum inhibitory concentration (MIC) test. Three isolates had an increase of MIC to aztreonam after exposure to both herbicides and one isolate did not show any MIC change. The MexAB-OprM efflux pump has already been upregulated in these isolates and the herbicides atrazine and diuron did not increase MexAB-OprM overexpression. Therefore, the decrease in aztreonam susceptibility was not directly related to this pump, suggesting that probably other mechanisms should be involved.     

Cytotoxicity of gamma irradiated aflatoxin B1 and ochratoxin A

Toxicity of gamma irradiated mycotoxins aflatoxin B₁ (AFB₁) and ochratoxin A (OTA) was investigated in vitro. AFB₁ and OTA stock solutions (50?mM, in methanol) were gamma irradiated (5 and 10 kGy) and non-irradiated and irradiated mycotoxins solutions were tested for cytotoxicity on Pk15, HepG2 and SH-SY5Y cell lines (MTT assay, 1–500?μM concentration range; 24?h exposure). Degradation of mycotoxin molecules was examined by liquid chromatography tandem mass spectrometry (HPLC-MS/MS). AFB₁ and OTA radiolytic products were less toxic than the parent mycotoxins to all of the tested cell lines. Gamma irradiation even at 5 kGy had effect on AFB₁ and OTA molecules however, this effect was dependent on chemical structure of mycotoxin. Since gamma irradiation at low dose reduced initial level of both mycotoxins, and gamma irradiated mycotoxins had lower toxicity in comparison to non-irradiated mycotoxins, it can be concluded that gamma irradiation could be used as decontamination method.     

Respiratory health assessment and exposure to polycyclic aromatic hydrocarbons in Mexican indigenous population

Environmental Science and Pollution Research - Indoor air pollution is an important risk factor for the generation of lung diseases in developing countries. The indigenous population is...     

Potential use of composts and vermicomposts as low-cost adsorbents for dye removal: an overlooked application

Environmental Science and Pollution Research - The use of composts and vermicomposts as adsorbents is an important topic of study in the field of environmental remediation. These materials are rich...     

Assessing the alteration of physicochemical characteristics in composted organic waste in a prototype decentralized composting facility

Environmental Science and Pollution Research - This article presents the pilot experience of an integrated biowaste management system developed in Tinos island, Greece, which promoted source...     

Antimicrobial Activity of Polyhexamethylene Guanidine Derivatives Introduced into Polycaprolactone

The research was aimed at determining the abundance of biofilm formation by Escherichia coli and Staphylococcus aureus on the surface of polycaprolactone (PCL) with polyhexamethylene guanidine (PHMG) derivatives and effect of the derivatives on extracellular hydrolytic enzymes and intracellular dehydrogenases. Biofilm abundance was determined by spectrophotometry, using crystal violet staining. Hydrolytic enzymes after contact with the film were determined with the use of non-specific substrate—fluorscein diacetate. The effect of PHMG derivatives on dehydrogenases activity was assessed using the test, where triphenyltetrazolium chloride (TTC) is reduced to triphenylformazan (TF). The PCL containing PHMG granular polyethylene wax and salt of sulfanilic acid (0.6–1% wt.) strongest inhibited biofilm formation. PHMG derivatives introduced into PCL were found to slightly affect hydrolases activity in both E. coli and S. aureus at a concentration of 0.2 and 0.6%. It was also found that dehydrogenases activity was inhibited by PCL films containing PHMG derivatives. PCL containing 1% of PHMG sulfanilate strongest inhibited hydrolases activity, whereas PCL modified with 1% of PHMG granular polyethylene wax showed the highest inhibitory effect on the activity of both enzymes. W-PCL and A-PCL composites (at concentration of 0.6%) have optimal combination of antibiofilm activity and biodegradability.     

Degradability Characterization of EPDM/IIR Blends by γ-irradiation

In this paper the modifications induced in butyl rubbers (pristine, chlorinated and brominated sorts) by γ-irradiation are investigated by swelling, chemiluminescence and FT-IR. The susceptibility of butyl rubbers for the generation of radicals orders their stabilities in the following sequence: IIR?>?IIR—Cl?>?IIR—Br. The incorporation of butyl rubbers into ethylene-propylene terpolymer matrix brings about increased densities of radicals initiating modifications in the oxidation state in respect with recombination, which are intensified as the processing dose increases. Based on the variation of carbonyl and hydroxyl indices the favorable route for the recycling EPDM based formulations would be suggested in this study. The chemiluminescence spectra proving the formation of peroxyl radicals at about 100?°C prove their availability as reclaiming solutions. IIR—Br is the recommendable butyl rubber for the recovery procedure by association with EPDM. The suitability of IIRs for recycling purposes is analyzed by the variation in their crosslink densities, free volumes and swelling degrees. The crosslinking behavior of stabilized EPDM/IIR blends that runs to the improvement of durability is depicted by Charlesby–Pinner representation, which involves the different simultaneous contribution of scission and crosslinking processes.     

Soils impacted by PAHs: Would the stabilized organic matter be a green tool for the immobilization of these noxious compounds?

The objective of this study was to investigate the role of stabilized organic matter (vermicompost) and tropical soils in the sorption of naphthalene, anthracene and benzo[a]pyrene. The results obtained for the three compounds were extrapolated for the priority polycyclic aromatic hydrocarbons (PAHs) pollutants according to Environmental Protection Agency (US EPA). To evaluate the sorption process, high performance liquid chromatography was employed and the data was fitted by Freundlich isotherms. The results suggest that the sorption effect generally increases with the number of benzene rings of the PAHs, and that the persistence of PAHs in the environment is possibly related to the number of benzene rings in the PAH molecule. In addition, the pH of the vermicompost can strongly affect the adsorption process in this matrix.     

Geophysical and hydrological data assimilation to monitor water content dynamics in the rocky unsaturated zone

In recent years, geophysics is increasingly used to study the flow and transport processes in the vadose zone. Particularly, when the vadose zone is made up of rocks, it is difficult to install sensors in the subsurface to measure hydrological state variables directly. In these cases, the electrical resistivity tomography (ERT) represents a useful tool to monitor the hydrodynamics of the infiltration and to estimate hydraulic parameters and state variables, such as hydraulic conductivity and water content. We propose an integrated approach aimed at predicting water content dynamics in calcarenite, a sedimentary carbonatic porous rock. The uncoupled hydrogeophysical approach proposed consists in 4D ERT monitoring conducted during an infiltrometer test under falling head conditions. Capacitance probes were installed to measure water content at different depths to validate the estimations derived from ERT. A numerical procedure, based on a data assimilation technique, was accomplished by combining the model (i.e., Richards’ equation) with the observations in order to provide reliable water content estimations. We have used a new data assimilation method that is easy to implement, based on the ensemble Kalman filter coupled with Brownian bridges. This approach is particularly suitable for strongly non-linear models, such as Richards’ equation, in order to take into account both the model uncertainty and the observation errors. The proposed data assimilation approach was tested for the first time on field data. A reasonable agreement was found between observations and predictions confirming the ability of the integrated approach to predict water content dynamics in the rocky subsoil.     

Effects of beach tourists on bathing water and sand quality at Puerto Velero,Colombia

Fragile coastal areas suffer from human activities. Environmental quality is one of the most important aspects in a tourist destination of sun and sand. The quality of bathing water and sand became indicators in the worldwide competition of beach destinations. We studied the water and sand quality along the beach of Puerto Velero, in northern Colombia. Water and sand beach quality were monitored during thirteen months. This allowed identifying the most significant sources of pollution along the beach, and understanding the interrelationship between tourism and the effects on the environment. Linear correlations allow assessing the association between the number of visitors and the physicochemical and microbiological parameters. The number of visitors was directly correlated with the presence of grease and oils, both in water and sand, as well as with fecal coliforms in water. A relation between the suspended solids and the presence of fecal coliforms in water and sand was observed. This statistical approach allows understanding the origin of beach sand and swimming water pollution at tourist beaches. Overall, sand quality has an important influence and is directly related to the water quality of the beach. Both the number of tourists and their activities are main factors explaining the contamination of the water and sand. This shows the importance of management of waste and wastewater in combination with awareness raising initiatives. More research should include monitoring and evaluating of both water and sand beach and provide data to revise the existing legal framework.