Partitioning of dioxins and dibenzofurans: Whole blood, blood plasma and adipose tissue

It has become relatively common to estimate human dioxin body burden and to document dioxin exposures by measuring dioxin and dibenzofuran congeners in adipose tissue, whole blood or blood plasma, and reporting these values on a lipid basis. It has not been determined whether these three types of specimens contain identical dioxin and dibenzofuran levels. This paper compares paired plasma and adipose tissue and paired whole blood and adipose tissue in analyses from two groups of patients. The first group consists of twenty U.S. veterans with paired plasma and adipose specimens. The second group consists of four German adults with whole blood compared to adipose tissue. Forty-eight analyses were performed. The results suggest that for some higher chlorinated compounds, such as OCDD, plasma lipid values may be higher than adipose lipid values, but whole blood lipid values for the higher as well as lower chlorinated PCDD/Fs may be relatively similar. On the other hand, the values for the lower chlorinated PCDD/Fs, such as TCDD, are similar in blood plasma, adipose tissue and whole blood. Total PCDD/F dioxin “toxic equivalents” are similar within each of the two series reported here, using the current “International Dioxin Toxic Equivalent” system.     

Environmental exposure to PCBs and cancer incidence in eastern Slovakia

In a population-based cross-sectional study, we compared serum levels of 15 polychlorinated biphenyl (PCB) congeners and three organochlorine pesticides in residents of two districts in eastern Slovakia, one with extensive environmental contamination from a former PCB production site (Michalovce) and the other matched on geography but with low (background) levels (Svidnik). The age-adjusted geometric means for sum of 15 measured PCB congeners were statistically significantly higher in subjects from the Michalovce district for both sexes: 3327.6 versus 1331.4 ng/g of lipid in males, 2751.8 versus 992.2 ng/g of lipid in females. Levels of DDE and DDT were also significantly higher, although the absolute differences were less pronounced. There was no substantial difference in HCB levels between the districts. We also conducted an ecologic study to compare cancer incidence occurring in these same two districts from 1985 through 1994. We calculated standardized incidence ratios (SIRs) and 95% confidence intervals for each district using indirect standardization and rates from eastern Slovakia. Among males from Michalovce (exposed), but not Svidnik (unexposed), there was an excess of cancer of the tongue (SIR=1.46; 1.06-1.96), stomach (SIR=1.15; 1.00-1.32), lung (SIR=1.14; 1.04-1.24), testis (SIR=1.40; 0.97-1.97), and kidney (SIR=1.23; 0.98-1.52), and lower than expected incidence of prostate cancer (SIR=0.83; 0.69-0.97); in contrast, there was an excess of peritoneal (SIR=3.05; 1.11-6.63) and laryngeal cancer (SIR=1.43; 0.99-1.98) in Svidnik not observed Michalovce. Among females from Michalovce, but not Svidnik, there was an excess of cancer of the lip (SIR=2.54; 1.53-3.96), stomach (SIR=1.22; 1.02-1.44), and lung (SIR=1.17; 0.94-1.45); in contrast, there was an excess of kidney (SIR=1.61; 1.03-2.40) and thyroid (SIR=1.97; 1.12-3.20) cancer in Svidnik not observed in Michalovce. Taken together, these results raise the possibility that high environmental exposure to organochlorines in the Michalovce district may be associated with higher rates of certain cancers, particularly stomach and lung cancer.     

The evolution of marmot sociality: II. Costs and benefits of joint hibernation

Summary Social groups of alpine marmots (Marmota marmota) were studied for 7 years. The groups consisted of a territorial pair and up to 18 lower ranking animals of various ages, mostly the pair's offspring (Tables 1, 2). Group members lived in a common home range and always hibernated together in one hibernaculum. Groups with older, subordinate animals experienced slightly higher summer mortality but significantly reduced winter mortality (Fig. 1). Infant winter mortality was further decreased if most older subordinates were potentially their full sibs (Fig. 2). Subordinate group members lost less mass during winter with increasing size of the hibernating group, but this trend was reversed when infants were present. Furthermore, augmented mass loss due to low hibernaculum quality became evident (Fig. 3). Apart from these effects, the presence of infants caused additional mass loss in potential full sibs. The opposite was found in subordinates certainly descending from other parents than those of the infants (Table 3).Winter mortality and mass loss data revealed (i) a general benefit of joint hibernation, (ii) an unavoidable cost of infants' presence to other group members, (iii) that only potential full sibs helped in warming infants, (iv) that helping was energetically expensive and increased infant survival. The evolution of postponed dispersal in ground-dwelling squirrels has been attributed to the direct fitness gained by enhanced reproductive chances of offspring when not expelled from the natal territory (Armitage 1981, 1987, 1988). This study shows that group living in alpine marmots has benefits during winter and indicates the additional importance of kin selection in marmot social evolution.     

Representing the Connectivity of Upland Areas to Floodplains and Streams in SWAT+

In recent years, watershed modelers have put increasing emphasis on capturing the interaction of landscape hydrologic processes instead of focusing on streamflow at the watershed outlet alone. Understanding the hydrologic connectivity between landscape elements is important to explain the hydrologic response of a watershed to rainfall events. The Soil and Water Assessment Tool+ (SWAT+) is a new version of SWAT with improved runoff routing capabilities. Subbasins may be divided into landscape units (LSUs), e.g., upland areas and floodplains, and flow can be routed between these LSUs. We ran three scenarios representing different extents of connectivity between uplands, floodplains, and streams. In the first and second scenarios, the ratio of channelized flow from the upland to the stream and sheet flow from the upland to the floodplain was 70/30 and 30/70, respectively, for all upland/floodplain pairs. In the third scenario, the ratio was calculated for each upland/floodplain pair based on the upland/floodplain area ratio. Results indicate differences in streamflow were small, but the relative importance of flow components and upland areas and floodplains as sources of surface runoff changed. Also, the soil moisture in the floodplains was impacted. The third scenario was found to provide more realistic results than the other two. A realistic representation of connectivity in watershed models has important implications for the identification of pollution sources and sinks.     

Inorganic arsenic: A non-genotoxic carcinogen

Inorganic arsenic induces a variety of toxicities including cancer. The mode of action for cancer and non-cancer effects involves the metabolic generation of trivalent arsenicals and their reaction with sulfhydryl groups within critical proteins in various cell types which leads to the biological response. In epithelial cells, the response is cell death with consequent regenerative proliferation. If this continues for a long period of time, it can result in an increased risk of cancer. Arsenicals do not react with DNA. There is evidence for indirect genotoxicity in various in vitro and in vivo systems, but these involve exposures at cytotoxic concentrations and are not the basis for cancer development. The resulting markers of genotoxicity could readily be due to the cytotoxicity rather than an effect on the DNA itself. Evidence for genotoxicity in humans has involved detection of chromosomal aberrations, sister chromatid exchanges in lymphocytes and micronucleus formation in lymphocytes, buccal mucosal cells, and exfoliated urothelial cells in the urine. Numerous difficulties have been identified in the interpretation of such results, including inadequate assessment of exposure to arsenic, measurement of micronuclei, and potential confounding factors such as tobacco exposure, folate deficiency, and others. Overall, the data strongly supports a non-linear dose response for the effects of inorganic arsenic. In various in vitro and in vivo models and in human epidemiology studies there appears to be a threshold for biological responses, including cancer.     

The Development of Air Contaminant Emission Tables for Nonprocess Emissions

In New York State, the calculation of air contaminant emissions from a variety of sources is an essential part of comprehensive air pollution studies. The tables used to calculate emissions were obtained from an extensive literature search and modified to apply to New York State conditions. For example, sulfur dioxide emission factors for coal were selected to reflect the average sulfur content of the coal sold in New York State. Since the literature contains a wide array of emission factors, it was necessary to evaluate the factors and select those which would be most appropriate for the techniques used in conducting the comprehensive studies in New York State. This paper does not present the emission tables themselves but does outline the development of such tables for use in nonprocess calculations, i.e., combustion for heat and power of bituminous and anthracite coal, distillate and residual oil, natural and bottled gas; combustion of gasoline and diesel in internal combustion engines; burning of refuse in dumps and incinerators; and evaporation of gasoline from marketing operations.     

Development of Sediment and Nutrient Export Coefficients for U.S. Ecoregions

Water quality impairment due to excessive nutrients and sediment is a major problem in the United States (U.S.). An important step in the mitigation of impairment in any given water body is determination of pollutant sources and amount. The sheer number of impaired waters and limited resources makes simplistic load estimation methods such as export coefficient (EC) methods attractive. Unfortunately ECs are typically based on small watershed monitoring data, which are very limited and/or often based on data collected from distant watersheds with drastically different conditions. In this research, we seek to improve the accuracy of these nutrient export estimation methods by developing a national database of localized EC for each ecoregion in the U.S. A stochastic sampling methodology loosely based on the Monte‐Carlo technique was used to construct a database of 45 million Soil and Water Assessment Tool (SWAT) simulations. These simulations consider a variety of climate, topography, soils, weather, land use, management, and conservation implementation conditions. SWAT model simulations were successfully validated with edge‐of‐field monitoring data. Simulated nutrient ECs compared favorably with previously published studies. These ECs may be used to rapidly estimate nutrient loading for any small catchment in the U.S. provided the location, area, and land‐use distribution are known.