In vitro and in vivo study of neutrophil functions after acute methanol intoxication in albino rats

Toxicity from methanol (MeOH), a potentially significant problem due to occupational, accidental, or intentional, as well as daily ingestion of small amounts of the agent, only receives considerable attention after severe signs of intoxication have set in or death is imminent. While accidental and intentional exposures usually involve very high doses, the occupational and ingestion forms more often reflect small daily intakes. Still, even at the low levels, little is known about the potential immunotoxic implications (and less so in regard to mechanisms) from these ongoing exposures. This study has been attempted to focus the effects of acute methanol exposure on blood neutrophil functions in both in vitro and in vivo conditions at 30 and 60?min time intervals. Innate immunity of the host defense relies mostly on neutrophils. The blood MeOH levels were determined upon exposing the experimental animals to ¼ of LD₅₀ MeOH. No significant change in the blood methanol level was observed at 30 and 60?min of methanol exposure. The in vitro results showed a decrease in the neutrophil functions of adherence and phagocytic index without affecting the avidity index, followed by an increased nitroblue tetrazolium (NBT) reduction. The in vivo exposures of MeOH showed a decline in neutrophil adherence. However, the other neutrophil functions were found to be significantly increased, which indicates that in vivo results are different from that of the in vitro studies. The present study concludes that MeOH exposure can reconstitute the innate immune status by altering the neutrophil functions. Moreover, the in vivo effects are much pronounced compared to the in vitro effects, which may be due to the formation of the metabolites under in vivo conditions.     

Role of GSH in the amelioration of chromium-induced membrane damage

Membrane damage is one of the important consequences of chromium (Cr), an environmental toxicant, induced cytotoxicity. Reduced glutathione (GSH), a membrane protectant may be used to reduce the Cr-induced membrane damage. In the present study, the impact of Cr in presence and absence of GSH was studied on plasma membrane of the liver and kidneys in male Wistar rats. Significant increases in membrane cholesterol levels as well as significant decreases in membrane phospholipid levels in Cr exposed (0.8 mg per 100 g body weight, i.p., for 28 days) animals suggest structural alterations in both the liver and kidney plasma membranes. Alkaline phosphatase (ALP), total ATPase, and Na⁺–K⁺–ATPase activities of plasma membrane were significantly decreased in both the liver and kidneys after Cr treatment. This treatment also produced significant weight loss and increased Cr content in the liver and kidneys. However, GSH (8 mg per 100g body weight, i.p., daily at an interval of 6 h after injection of Cr for a period of 28 days) supplementation restored alterations induced by Cr in plasma membrane of both the liver and kidneys but was not able to eliminate the deposited Cr from the liver and kidney tissues.     

Toxicity of endosulfan on growth,photosynthesis, and nitrogenase activity in two species of Nostoc (Nostoc muscorum and Nostoc calcicola)

Treatments of the cyanobacteria Nostoc muscorum and Nostoc calcicola with the insecticide endosulfan (5, 10, and 20?µg?mL^?1) inhibited growth, photosynthetic pigments, photosynthetic, and nitrogenase activities. The sensitivity of N. muscorum to endosulfan was higher than that of N. calcicola. The toxic effect of endosulfan was more pronounced on phycocyanin; however, a considerable reduction in chlorophyll a and carotenoids was also noticed. ¹⁴C-fixation appeared to be more sensitive to the insecticide than photosynthetic oxygen evolution. Endosulfan caused strong inhibition of photosystem (PS) II activity whereas PS I was least affected. The inhibition of PS II activity was partially restored by electron donors (DPC, NH₂OH, and MnCl₂) at low dose of endosulfan. Nitrogenase activity was significantly suppressed in both species by the endosulfan at high dose (20?µg?mL^?1). On the basis of our comparative analysis, N. calcicola was found to be endosulfan resistant and can be used in paddy fields for better productivity under pesticide stress.     

Comparative studies of liver and brain glycogen content of male and female Clarias batrachus (L.) after exposure of different doses of arsenic

Different doses of arsenic (As) were used to investigate comparative toxicity on the liver and brain glycogen content on male and female Indian catfish Clarias batrachus (L.). As-induced effects were associated with gender, dose (5, 10, or 15?mg?L^?1), and varying time periods (48, 96, or 144?h). It was noted that As produced dose- and time-dependent liver glycogenolysis and late brain glycogenolysis. Liver glycogenolysis was significantly increased after 48?h at all three As doses. At the highest dose 15?mg?L^?1, liver glycogen were markedly diminished at in both male and female fish, but in females more reduction was observed than in males. However, with brain glycogen, the significant decrease was noted at 144?h with all three dose levels in both genders, with male being more susceptible. Thus, this study indicates that As produces glycogenolysis. The reduction in the liver glycogen content was more pronounced in female than in the male fish, whereas brain glycogen content decrease was more prominent in males.     

Immunotoxic manifestations in rats following inhalative exposure to furfural vapors

Furfural is an industrial compound used as a process intermediate and as a solvent; it poses a potential inhalation hazard in occupational settings. This study was carried out to find furfural-induced immunotoxicity in Wistar rat following inhalative exposure. The weights of thymus and lymph node were found decreased, while the weights of the liver and the adrenal gland were significantly increased following furfural exposure. Delayed-type hypersensitivity response was found decreased in furfural vapors-exposed animals when compared to that of control animals. The phagocytic index of peritoneal and alveolar exudates showed significant decrease and was most prominent (90%) in 30 days-exposed groups. The number of anti-rat anti-sheep red blood cell immunoglobulin M plaque forming cells of spleen got decreased in furfural-exposed groups in comparison to control. Taken together, this study indicates that inhalation of furfural induces immunotoxic manifestations that could lead to severe immunological disorders.     

Effects of the level of dietary protein on the toxicity of hexachlorocyclohexane in rats

Male albino rats were fed for 28 days from weaning on diets containing 5% (group 1), 10% (group 2) and 21% (group 3, normal protein) protein as casein. At the end of dietary period, HCH was administered daily for 30 days to investigate the interaction between protein deficiency and pesticide toxicity. The results indicated that rats fed a lower protein diet and HCH had a higher mortality, lower rate of growth, increased liver weight and deposition of the pesticide in blood and tissues in larger amounts. Blood pressure (systolic and diastolic) was significantly increased and the heart rate showed tachycardia in low protein exposed animals. A significant increase of total lipids, cholesterol, triglycirides, free fatty acids in serum and tissues of animals exposed to low protein was observed. A close correlation existed between lipid accumulation and storage of HCH in tissues and dietary protein seemed to play an important role in detoxification.     

Photodegradation of the organophosphorus herbicide “anilophos”

Photodecomposition of the organophosphorus herbicide anilophos [S‐(N‐(4‐chlorophenyl) N‐isopropyl carbaniloyl methyl] 0,0‐dimethyl phosphorodithioate has been studied in methanol. It underwent oxidation, hydrolysis and dimerisation on irradiation with U.V. light and produced a number of photoproducts which were characterised by ¹H‐NMR and Mass spectroscopy.     

Photodegradation of the organophosphorus insecticide ‘Phorate’

The photolysis of Phorate(I) (0,0‐diethyl S‐ethyl thiomethyl phosphordithioate) has been studied as a thin film on a glass surface and in a solution of methanol‐water (60:40) by ultraviolet light (λ > 290 nm). The rate of disappearance of Phorate in the solution show first order Kinetics with a rate constant of 4.9 × 10^–5 S ^–1. The half‐life of (I) exposed on a glass surface is found to be 5 hours. The structure of the major photoproducts were characterised by ¹H NMR and mass spectroscopy.     

Historical evidence of climatic variability and changes,and its effect on high-altitude regions: insights from Rara and Langtang,Nepal

ABSTRACT

Climatic variability and its effects have been experienced in the high-altitude regions of Nepal for some considerable time. Most of the studies on local people’s perception available so far in Nepal on climate include with respect to weather changes, and almost none have been verified with satellite imagery. This study thus attempts to combine meteorological and satellite imagery for comparing local people’s perception so that a more robust validation can be established. Both qualitative (transect walk, key informant interview, focus group discussion and institutional visit) and quantitative (meteorological and satellite image) data and techniques were employed. Local people from Rara and Langtang in Nepal shared their observations and perceptions on the changing climate for the last three decades and the effects on them and their local microclimate. Apart from temperature, rainfall and snowfall anomalies, locals observed changes in the water sources and increasing drought along with alteration in the phenology of tree and agricultural crops as well as vegetation range migration. Satellite image analysis also confirms a change in snow cover as notified by the local people. This study shows that local people’s knowledge could be considered as a complement to the observed scientific evidences of climate change science and their perceptions can be used reliably where scientific data are lacking. Finally, perceived climatic risks, current gaps and future opportunities are discussed and some recommendations are suggested.     

Community structure of zooplankton across environmental variables in a high-altitude lake of central Himalayas,India

We determined seasonal and spatial variation of zooplankton and their correlation with environment variables in a subtropical lake of the Himalayas. Thirty-eight species of zooplankton were identified, of which, 38.0% belonged to rotifera, 26.3% to cladocera, and 15.7% to copepoda. Nitrogen nutrients, total organic matter (TOM), and clay particles significantly influenced on the distribution pattern of zooplankton. Non-metric multidimensional scaling analysis showed homogeneous assemblages of dominant zooplankton species except Chydorus gibbus which has wider range of niche partitioning. Turbidity formed by suspended materials facilitated better avoidance of larger zooplankton like Daphnia similis from predators. Sedimentary silt particles influenced on the assemblages of sensitive species such as Tropocyclops prasinus, Asplanchna priodonta and Colurella obtuse. Changes within the land-use pattern in the catchment of the central Himalayan lakes either directly or indirectly impact on the distribution of zooplankton community.