Simultaneous determination of malachite green, enrofloxacin and ciprofloxacin in fish farming water and fish feed by liquid chromatography with solid-phase extraction

An effective and sensitive method for simultaneous analysis of malachite green (MG), enrofloxacin (ENFLX) and ciprofloxacin (CPFLX) by liquid chromatography-diode array detection with solid-phase extraction (SPE) is developed. The conditions of SPE and LC were investigated and optimised. The effective separation of these compounds was achieved using a ZY1104 C18 column (250 × 4.6 mm, 5 μm) with 20 mM tetrabutyl ammonium bromide (pH 3.0)-acetonitrile as mobile phase and gradient elution. The diode array detection was used at 278 nm for ENFLX and CPFLX and at 613 nm for MG. Under the optimal conditions, the method LOD values of MG, ENFLX and CPFLX were 0.01, 0.07 and 0.10 μg L(?-1) for fish farming water samples and 1.5, 10.5 and 15 μg kg(?-1) for fish feed samples, respectively. The relative recoveries of the three analytes were achieved to be 76.7-82.3% with the RSDs (n = 5) of 3.2-4.6% for spiked fish farming water samples and 78.8-93.7% with the RSDs (n = 5) of 3.1-4.8% for spiked fish feed samples.     

硫丹对罗非鱼(Oreochromis aureus×O.niloticus)和草鱼(Ctenopharyngodon idellus)的急性毒性研究

为控制虫害农业上大量使用硫丹,因此硫丹可通过多种途径进入水环境中.养殖环境和水产品中已发现硫丹残留,这给养殖环境和食品安全造成潜在威胁.通过半静态试验方法测定了罗非鱼和草鱼的半致死浓度,并估算了其在水环境中的安全浓度.结果表明,罗非鱼和草鱼的半致死浓度分别为1.97(1.26 -2.87) μg·L-1和2.33(1....     

1695年临汾8级大震成因讨论

根据动态传播断层与同层内横向断层间的相互作用以及异层内深浅断层之间的相互作用讨论了1303年洪洞大震时北北东向震源大幅度错动对临汾北西西向活动断层的致锁作用,由此研究了1695年临汾8级大震形成的原因。最后还讨论了1989年山西大同—阳高中强震发生的成因机制。     

模糊层次综合法用于企业安全性评价

将模糊综合评价与层次分析相结合 ,建立了企业安全模糊层次综合法的评价模型。实践证明 ,这种方法在进行企业的安全性综合评价中是有效的     

漫湾库区生产恢复障碍及其原因

在对漫湾库区调查的基础上，总结了库区生产恢复现状，认为农业生产相应条件恢复不到位，影响土地的持续利用：土地生产力降低，单一的种植业结构和产业结构，经济林与畜牧养殖业因土地被淹没带来萎缩，渔业、旅游业等缺乏规划和移民的参与，表现出库区移民生活水平相对于远迁移民、全县(省)平均下降了。分析了漫湾库区生产恢复的障碍及其原因，并就生产恢复提出一些建议：(1)提升澜沧江梯级开发水电工程中对移民问题的重视，将漫湾电站纳人中央直属电站范畴，统筹安排移民遗留问题，给予资金支持；(2)重新整体规划漫湾库区的移民安置，控制库区土地人口压力；(3)调整产业结构，促成传统种植业人口向林、牧业，向第二、三产业转移；(4)理顺和明确移民方面的责、权、利关系，将生产恢复问题与安置区地方经济发展问题结合起来考虑；(5)建立公众积极参与的决策体制与决策执行机制。     

冶金矿业环境隐患因子识别

本文分析了环境隐患与安全隐患的异同,定义了冶金矿业行业环境隐患的含义,分析了环境隐患与环境事故的关系,提出环境隐患因子识别的原则、范围和方法。根据冶金矿业行业实际生产情况,用LEC法和危险物质辨识方法对冶金矿业环境隐患进行识别,最终指出常见的冶金矿业环境隐患因子。     

黄河流域土壤侵蚀潜在风险及其转化分析

作者在前期研究中,基于流域最小图斑的划分,提出了土壤侵蚀快速评估方法,并在黄河流域的应用中取得了较满意的效果。论文以黄河流域土壤侵蚀强度的评估结果为基础,评估了流域土壤侵蚀的脆弱性。以抗侵蚀年限表征流域土壤侵蚀潜在危险度,结合土壤侵蚀脆弱性分析了流域土壤侵蚀潜在风险。通过与土壤侵蚀强度的对比,指出在流域水土保持和生态保护中,同时考虑土壤侵蚀强度和水土流失潜在风险才能作出更合理的决策。最后,分析了黄河流域中游土壤侵蚀风险向下游洪水风险的转化关系,指出黄河中游高产沙区土壤侵蚀风险转化为下游更高的洪水风险,在泥沙灾害研究中应关注灾害的链式反应。     

Characterization of depth-related microbial communities in lake sediment by denaturing gradient gel electrophoresis of amplified 16S rRNA fragments

The characterization of microbial communities of different depth sediment samples was examined by a culture-independent method and compared with physicochemical parameters, those are organic matter （OM）, total nitrogen （TN）, total phosphorus （TP）, pH and redox potential （Eh）. Total genomic DNA was extracted from samples derived from different depths. After they were amplified with the GC-341 f/907r primer sets of partial bacterial 16S rRNA genes, the products were separated by denaturing gradient gel electrophoresis （DGGE）. The profile of DGGE fingerprints of different depth sediment samples revealed that the community structure remained relatively stable along the entire 45 cm sediment core, however, principal-component analysis of DGGE patterns revealed that at greater sediment depths, successional shifts in community structure were evident. The principle coordinates analysis suggested that the bacterial communities along the sediment core could be separated into two groups, which were located 0-20 cm and 21-45 cm, respectively. The sequencing dominant bands demonstrated that the major phylogenetic groups identified by DGGE belonged to Bacillus, Bacterium, Brevibacillus, Exiguobacterium, γ-Proteobacterium, Acinetobacter sp. and some uncultured or unidentified bacteria. The results indicated the existence of highly diverse bacterial community in the lake sediment core.     

线性拟合在工程地震勘察技术中的应用

本文介绍利用最小二乘法建立线性拟合模型的过程,并给出一个利用一元线性拟合,实现用相遇观测时距曲线求V2的实际应用。同时给出Mathematica程序,实现此类数据处理的自动化,克服了人工处理工作量大、速度慢、繁琐之不足,更重要的是避免了人工处理时出错情况的发生。     

工业固定资产投资项目节能分析探讨

节能分析篇（章）在工业固定资产投资项目节能评估和审查中占有重要的地位,节能分析篇（章）是否全面、是否规范不仅决定了项目节能评估和审查的最终结论,也对项目投运后的能源管理产生着决定的作用。文章针对某重型机械有限公司大型高效水、火、核电及轧辊等大型轴类锻件加工基地建设项目节能分析情况进行了探讨。