Effects of environmentally relevant concentrations of metallic compounds on the flatfish Scophthalmus maximus: biomarkers of neurotoxicity,oxidative stress and metabolism

Flatfish species, such as the turbot (Scophthalmus maximus), are common targets for toxic effects, since they are exposed through the food chain (ingestion of contaminated preys) and are in direct contact with the waterborne contaminant and sediments. Furthermore, these fish species live in close proximity to interstitial water that frequently dissolves high amounts of contaminants, including metals. Despite this significant set of characteristics, the present knowledge concerning flatfish contamination and toxicity by metals is still scarce. To attain the objective of assessing the effects of metals on a flatfish species, S. maximus specimens were chronically exposed to lead, copper and zinc, at ecologically relevant concentrations, and biochemical (oxidative stress: catalase and glutathione S-transferases activities, and lipid peroxidation; neurotoxicity: cholinesterase activity) parameters were assessed on selected tissues (gills and liver). Copper had no significant effects on all tested parameters; lead was causative of significant increases in liver GSTs activities and also in lipoperoxidation of gill tissue; exposure to zinc caused a significant increase in catalase activity of gill tissue. None of the tested metals elicited noteworthy effects in terms of neurotoxicity. The obtained results showed that only the metal lead is of some environmental importance, since it was able to cause deleterious modifications of oxidative nature at relevant concentrations.     

Biochemical effects of acetaminophen in aquatic species: edible clams Venerupis decussata and Venerupis philippinarum

Acetaminophen (paracetamol) is one of the most used pharmaceutical drugs, due to its antipyretic and analgesic properties that turn it into a primary choice in varied pathologies and conditions. However, and despite its massive use, acetaminophen is not exempt of adverse effects, especially when administered in over dosage, which are related to the formation of toxic metabolites by oxidative pathways. It is thus possible to observe that toxicity caused by acetaminophen is usually mediated by reactive oxygen species and can result in multiple effects, ranging from protein denaturation to lipid peroxidation and DNA damage. The occurrence of acetaminophen has been reported in the aquatic environment, being important to address the potential exertion of toxic effects on nontarget environmentally exposed organisms. The present study intended to characterize the effects of acute acetaminophen exposure on physiological traits (antioxidant defense, oxidative damage) of two species of bivalves, namely, the edible clams Venerupis decussata and Venerupis philippinarum. Results showed a significant increase in all oxidative stress biomarkers, evidencing the bioactivation of acetaminophen into a deleterious prooxidant, triggering the onset of deleterious effects. Furthermore, strong interspecific differences were observed among responses of the two tested species, which was a major issue due to intrinsic ecological implications when one considers that both species share the same habitat.     

Tillage system did not affect weed diversity in a 23-year experiment in Mediterranean dryland

This study investigated whether the choice of a tillage system (no-tillage, minimum tillage or traditional tillage) affected weed diversity in a 23 years cereal–leguminous rotation system in Spain. Weed diversity was assessed using common diversity indices: species richness, Shannon's index and Pieloús evenness. Linear mixed-effects models were employed to compare the tillage systems. It was found that after 23 years no large differences between tillage systems have arisen related to weed diversity. Only minimum tillage appeared to support, on average, more species than the two other tillage systems. Richness, Shannon diversity index and evenness varied largely through the years in all tillage systems but this variation was not related to type of crop sown (cereal or leguminous). Our results highlight that conservation tillage practices did not represent any concern for weed diversity conservation in cereal–leguminous rotations in the conditions of central Spain.     

A study about remedial measures to reduce 222Rn concentration in an experimental building

Radon gas emanating from underground can spread to adjoining closed areas. It can concentrate and reach levels which represent a risk to people's health. It is well known that radon presence in most areas depends mainly on the area's geological features. Indoor radon concentrations further depend on the type of structure, construction materials and the technology used for the building. Therefore, indoor radon monitoring is of primary importance for deciding whether remedial measures are to be adopted for reducing harmful concentrations. This approach has been tried by measuring radon concentration in an experimental building situated in Milan (Italy). This building situated in a geological area that is considered at low radon risk. The results were obtained after analysing radon concentration in indoor rooms, crawl spaces, soil gas and in the atmosphere outside and by measuring before and after adoption of remedial measures. The study shows that improper building design can give rise to higher indoor radon accumulation even in an area of poor radon exhalation. Furthermore, the results enable quantification of the effectiveness of the remedial measures.     

Forest Perception and Knowledge of Hikers and Mountain Bikers in Two Different Areas in Northwestern Switzerland

Recreational activities can have major impacts on vegetation and wildlife in frequently visited forests. We assessed forest perception and knowledge (state, functions, and species diversity) among hikers and mountain bikers in a frequently visited, seminatural suburban recreation forest (Muttenz) and in a more distantly situated, naturally grown excursion forest (Wasserfallen) in northwestern Switzerland. In all, 239 hikers and 126 mountain bikers were interviewed. Mountain bikers in both forests and hikers in the more intensely used recreation forest at Muttenz assessed the state of the forest less optimistically and showed a higher awareness of the negative impact of recreational activities on the flora and fauna than hikers at Wasserfallen. Furthermore, mountain bikers seemed aware of the social conflicts caused by their activity, since they appreciated neutral or positive encounters with other forest visitors. In contrast, 57% of hikers at Muttenz reported on negative experiences with other forest visitors, particularly with mountain bikers. In general, the interviewees ecological and biological forest knowledge (forest type and function, species diversity) was rather high. A large proportion was aware of the pros and cons of different forest conditions for plants and animals, and could name or recognize at least some plant and/or animal species typical for the visited forest. The forest knowledge was neither influenced by the type of recreational activity carried out nor by any aspect of forest visit behavior (frequency and duration of forest visits, means of transportation and travel distance to forest). However, the interviewees forest knowledge was influenced by their age and educational level.     

Using non-equilibrium biosorption column modelling for improved process efficiency

An unsteady-state biosorption column model in one space variable is considered in this paper. The theoretical study is motivated by the need for predicting the dependent variables of biosorption columns for removal of heavy metals from wastewater, which is potentially an important technology in cleaner production. Pollutant concentrations in the bulk phase, in the liquid filling the pores and in the solid biomass along the axial coordinate of the column were evaluated for a wide range of physical and chemical parameters of the global process. In particular, the assumption of instantaneous chemical reactions is replaced by a more realistic expression taking into account chemical kinetics and fluid advection time scales separately. The resulting system of partial differential equations was solved by means of a reliable numerical algorithm based on the method of lines. The data obtained using the model described in this paper are compared with published experimental data and an estimation of the increased efficiency is made.     

Yield response of potato to spatially patterned nitrogen application

Although crop response to nitrogen fertilization has long been studied, classical experimental designs have led to inadequate accounting of spatial variability in field properties and yield response. Analytical methods to explicitly account for spatial variability now exist but the complementary modification of experimental design is still developing. There is a need to combine these analytical methods with non-traditional experimental design. A 2-year study was implemented to assess the response of potato (Solanum tuberosum cv. Kennebec) yield to nitrogen fertilizer rate. We used a transect-type plot design where four nitrogen treatments (0, 56, 112, and 280 kg N ha^?1) were applied systematically in a continuous sinusoidal pattern along longitudinal transects. Measured field properties included topography, soil texture, pre-application soil nitrate levels, and plant available soil water content. A random field linear model was used to simultaneously account for treatment effects and soil properties. The results showed that treatment effects were significantly different from each other; however, if spatially correlated errors were accounted for, these differences were smaller and significance levels lower. Nitrogen response functions varied widely throughout the field. Of the covariates, only clay content proved important in explaining spatial differences in response to N. The sinusoidal response pattern of N was similar over the 2 years but the amplitudes varied due to differences in weather. Interactions between uncharacteristically high rainfall and a sandy field soil may have minimized discernable effects of the other covariates. The results demonstrated how the statistical analysis of potato response to a patterned application of nitrogen fertilizer can take advantage of spatial correlations to understand the response of potato to nitrogen application over larger areas.