Impact of water quality on bacterioplankton assemblage along Cértima River Basin (central western Portugal) assessed by PCR–DGGE and multivariate analysis

The information on bacterial community composition (BCC) in Portuguese water bodies is very scarce. Cértima River (central western Portugal) is known to have high levels of pollution, namely organic. In the present work, the BCC from a set of 16 water samples collected from Cértima River Basin and its main tributaries was characterized using 16S rDNA–denaturing gradient gel electrophoresis, a culture-independent molecular approach. Molecular data were related to environmental parameters through multivariate analysis to investigate potential impact of water pollution along the river. Principal component analysis using environmental data showed a water quality gradient from more pristine waters (at the mountain tributaries) to waters with increasingly eutrophic potential (such as Fermentelos Lake). This gradient was mainly defined by factors such as organic and inorganic nutrient sources, electrical conductivity, hydrogen carbonate concentration, and pH. Molecular results showed variations in BCC along Cértima River Basin but in the main river section, a Bacteroidetes phylotype (Flavobacterium sp.) proved to be dominant throughout the river course. Multivariate analysis suggests that spatial variation of BCC along the Cértima River Basin depended mainly on parameters such as Chl a, total suspended solid (TSS), total organic carbon, electrical conductivity, and HCO _boxclose^-_{3}^{-} levels. Bacteroidetes phylotypes were all related to higher electrical conductivity and HCO₃^-_{3}^{-} levels although some of these were also correlated with high SO₄^2-_{4}^{2-} and others with high soluble reactive phosphorus, nitrate, TN, and Kjeld-N levels. The Gammaproteobacteria occurrence was correlated with high SO₄^2-_{4}^{2-} levels. One of the Betaproteobacteria phylotypes showed to correlate with low redox potential (E_h) and high temperature, pH, TSS, and Chl a levels while another one showed a negative correlation with Chl a values.     

Optimal Expansion Strategy for a Sewer System under Uncertainty

Because of fast urban sprawl, land use competition, and the gap in available funds and needed funds, municipal decision makers and planners are looking for more cost-effective and sustainable ways to improve their sewer infrastructure systems. The dominant approaches have turned to planning the sanitary sewer systems within a regional context, while the decentralized and on-site/cluster wastewater systems have not overcome the application barriers. But regionalization policy confers uncertainties and risks upon cities while planning for future events. Following the philosophy of smart growth, this paper presents several optimal expansion schemes for a fast-growing city in the US/Mexico borderlands—the city of Pharr in Texas under uncertainty. The waste stream generated in Pharr is divided into three distinct sewer sheds within the city limit, including south region, central region, and north region. The options available include routing the wastewater to a neighboring municipality (i.e., McAllen) for treatment and reuse, expanding the existing wastewater treatment plant (WWTP) in the south sewer shed, and constructing a new WWTP in the north sewer shed. Traditional deterministic least-cost optimization applied in the first stage can provide a cost-effective and technology-based decision without respect to associated uncertainties system wide. As the model is primarily driven by the fees charged for wastewater transfer, sensitivity analysis was emphasized by the inclusion of varying flat-rate fees for adjustable transfer schemes before contracting process that may support the assessment of fiscal benefits to all parties involved. Yet uncertainties might arise from wastewater generation, wastewater reuse, and cost increase in constructing and operating the new wastewater treatment plant simultaneously. When dealing with multiple sources of uncertainty, the grey mixed integer programming (GIP) model, formulated in the second stage, can further allow all sources of uncertainties to propagate throughout the optimization context, simultaneously leading to determine a wealth of optimal decisions within a reasonable range. Both models ran for three 5-year periods beginning in 2005 and ending in 2020. The dynamic outputs of this analysis reflect the systematic concerns about integrative uncertainties within this decision analysis, which enable decision makers and stakeholders to make all-inclusive decisions for sanitary sewer system expansion in an economically growing region.     

Biomonitoring of heavy metals (Cd,Hg, and Pb) and metalloid (As) with the Portuguese common buzzard (Buteo buteo)

The accumulation of heavy metals in the environment may have a wide range of health effects on animals and humans. Thus, in this study, the concentrations of arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg) in the blood and tissues (liver and kidney) of Portuguese common buzzards (Buteo buteo) were determined by inductively coupled plasma-mass spectrometer (ICP-MS) in order to monitor environmental pollution to these elements. In general, Hg and As were the elements which appeared in the highest and lowest concentrations, respectively. A highest percentage of non-detected concentration was found for blood Cd (94.6 %) but, in turn, it was the only metal that was detected in all kidney samples. The kidney was the analyzed sample which showed the highest concentrations of each element evaluated. Statistically, significant differences among blood, liver, and kidney samples were observed for As and Cd (P?相似文献   

Combination effects of anticholinesterasics in acetylcholinesterase of a fish species: effects of a metallic compound,an organophosphate pesticide,and a pharmaceutical drug

The simultaneous presence of distinct compounds in the aquatic environment can be causative of various toxicological interactions. This scenario challenges ecotoxicologists, since the assessment of toxicological effects caused by the simultaneous presence of multiple substances is by far more complicated. An illustrative example can be given by mentioning the anticholinesterasic compounds: by studying the level of cholinesterase impairment of an exposed organism, it is possible to ascertain the level of exposure to all anticholinesterasics (despite their chemical classes and natures) that the organism was subjected to. In this work, we describe the effects of three chemically different, albeit mechanistically, and toxicologically similar compounds (copper, chlorfenvinphos, and pyridostigmine) on cholinesterases of the fish Gambusia holbrooki. The results showed that the combinatorial effects may be of considerable extent, even for levels of exposure that are close to the ones already reported in the wild, for each isolated compound.     

Effects of environmentally relevant concentrations of metallic compounds on the flatfish Scophthalmus maximus: biomarkers of neurotoxicity,oxidative stress and metabolism

Flatfish species, such as the turbot (Scophthalmus maximus), are common targets for toxic effects, since they are exposed through the food chain (ingestion of contaminated preys) and are in direct contact with the waterborne contaminant and sediments. Furthermore, these fish species live in close proximity to interstitial water that frequently dissolves high amounts of contaminants, including metals. Despite this significant set of characteristics, the present knowledge concerning flatfish contamination and toxicity by metals is still scarce. To attain the objective of assessing the effects of metals on a flatfish species, S. maximus specimens were chronically exposed to lead, copper and zinc, at ecologically relevant concentrations, and biochemical (oxidative stress: catalase and glutathione S-transferases activities, and lipid peroxidation; neurotoxicity: cholinesterase activity) parameters were assessed on selected tissues (gills and liver). Copper had no significant effects on all tested parameters; lead was causative of significant increases in liver GSTs activities and also in lipoperoxidation of gill tissue; exposure to zinc caused a significant increase in catalase activity of gill tissue. None of the tested metals elicited noteworthy effects in terms of neurotoxicity. The obtained results showed that only the metal lead is of some environmental importance, since it was able to cause deleterious modifications of oxidative nature at relevant concentrations.     

Biochemical effects of acetaminophen in aquatic species: edible clams Venerupis decussata and Venerupis philippinarum

Acetaminophen (paracetamol) is one of the most used pharmaceutical drugs, due to its antipyretic and analgesic properties that turn it into a primary choice in varied pathologies and conditions. However, and despite its massive use, acetaminophen is not exempt of adverse effects, especially when administered in over dosage, which are related to the formation of toxic metabolites by oxidative pathways. It is thus possible to observe that toxicity caused by acetaminophen is usually mediated by reactive oxygen species and can result in multiple effects, ranging from protein denaturation to lipid peroxidation and DNA damage. The occurrence of acetaminophen has been reported in the aquatic environment, being important to address the potential exertion of toxic effects on nontarget environmentally exposed organisms. The present study intended to characterize the effects of acute acetaminophen exposure on physiological traits (antioxidant defense, oxidative damage) of two species of bivalves, namely, the edible clams Venerupis decussata and Venerupis philippinarum. Results showed a significant increase in all oxidative stress biomarkers, evidencing the bioactivation of acetaminophen into a deleterious prooxidant, triggering the onset of deleterious effects. Furthermore, strong interspecific differences were observed among responses of the two tested species, which was a major issue due to intrinsic ecological implications when one considers that both species share the same habitat.     

Tillage system did not affect weed diversity in a 23-year experiment in Mediterranean dryland

This study investigated whether the choice of a tillage system (no-tillage, minimum tillage or traditional tillage) affected weed diversity in a 23 years cereal–leguminous rotation system in Spain. Weed diversity was assessed using common diversity indices: species richness, Shannon's index and Pieloús evenness. Linear mixed-effects models were employed to compare the tillage systems. It was found that after 23 years no large differences between tillage systems have arisen related to weed diversity. Only minimum tillage appeared to support, on average, more species than the two other tillage systems. Richness, Shannon diversity index and evenness varied largely through the years in all tillage systems but this variation was not related to type of crop sown (cereal or leguminous). Our results highlight that conservation tillage practices did not represent any concern for weed diversity conservation in cereal–leguminous rotations in the conditions of central Spain.     

Vacuum pyrolysis process for remediation of hydrocarbon-contaminated soils

A vacuum pyrolysis process was used for the remediation of hydrocarbon–contaminated soils from “Les Vidangeurs de Montréal” site in Mascouche in the province of Québec, near Montréal. Ten samples were tested on a laboratory scale batch reactor, and one sample was tested on a prototype process development unit. The process is simple, efficient, reliable, and economically competitive with other existing technologies. The vacuum pyrolysis process efficiently treated soils contaminated with a variety of pollution levels and types, irrespective of the soil matrix, providing treated soils meeting the A criterion (noncontaminated, residential level) of the Ministry of Environment Québec (MENVIQ). The pyrolytic oil and the noncondensable pyrolytic gases can be used as makeup fuel in the process, because they have a high calorific value and their combustion should not pose any emission problems. The waterphase effluent must be treated before discharge.