Methanotrophs and methanotrophic activity in engineered landfill biocovers

The dynamics and changes in the potential activity and community structure of methanotrophs in landfill covers, as a function of time and depth were investigated. A passive methane oxidation biocover (PMOB-1) was constructed in St-Nicéphore MSW Landfill (Quebec, Canada). The most probable number (MPN) method was used for methanotroph counts, methanotrophic diversity was assessed using denaturing gradient gel electrophoresis (DGGE) fingerprinting of the pmoA gene and the potential CH₄ oxidation rate was determined using soil microcosms. Results of the PMOB-1 were compared with those obtained for the existing landfill cover (silty clay) or a reference soil (RS). During the monitoring period, changes in the number of methanotrophic bacteria in the PMOB-1 exhibited different developmental phases and significant variations with depth. In comparison, no observable changes over time occurred in the number of methanotrophs in the RS. The maximum counts measured in the uppermost layer was 1.5 × 10⁹ cells g dw^?1 for the PMOB-1 and 1.6 × 10⁸ cells g dw^?1 for the RS. No distinct difference was observed in the methanotroph diversity in the PMOB-1 or RS. As expected, the potential methane oxidation rate was higher in the PMOB-1 than in the RS. The maximum potential rates were 441.1 and 76.0 μg CH₄ h^?1g dw^?1 in the PMOB and RS, respectively. From these results, the PMOB was found to be a good technology to enhance methane oxidation, as its performance was clearly better than the starting soil that was present in the landfill site.     

Testing the feasibility of a hypothetical whaling‐conservation permit market in Norway

A cap‐and‐trade system for managing whale harvests represents a potentially useful approach to resolve the current gridlock in international whale management. The establishment of whale permit markets, open to both whalers and conservationists, could reveal the strength of conservation demand, about which little is known. This lack of knowledge makes it difficult to predict the outcome of a hypothetical whale permit market. We developed a bioeconomic model to evaluate the influence of economic uncertainty about demand for whale conservation or harvest. We used simulations over a wide range of parameterizations of whaling and conservation demands to examine the potential ecological consequences of the establishment of a whale permit market in Norwegian waters under bounded (but substantial) economic uncertainty. Uncertainty variables were slope of whaling and conservation demand, participation level of conservationists and their willingness to pay for whale conservation, and functional forms of demand, including linear, quadratic, and log‐linear forms. A whale‐conservation market had the potential to yield a wide range of conservation and harvest outcomes, the most likely outcomes were those in which conservationists bought all whale permits.     

Applying chemical oxidation technologies at NAPL‐impacted sites to facilitate risk‐based closure

In situ chemical oxidation (ISCO) has found widespread remedial application at sites that lack nonaqueous‐phase liquid (NAPL) or have a relatively small amount of contaminant mass. Historically, its use has been limited at sites with large amounts of NAPL, primarily because of cost considerations. Proper application of ISCO can expand its use at sites with substantial amounts of NAPL—particularly where it is being used to selectively remediate higher toxicity fractions or reduce the mobility of the NAPL itself through artificial weathering. Alone or in conjunction with conventional technologies, chemical oxidation provides a means for reducing the risk associated with NAPL and potentially closing impacted sites without completely removing NAPL. © 2010 Wiley Periodicals, Inc.     

Suppressed recovery of plant community composition and biodiversity on dredged fill of a hurricane-induced inlet through a barrier island

In September 2003, Hurricane Isabel created an inlet over 500 m wide and 10 m deep that connected the Atlantic Ocean and Pamlico Sound. This breach was subsequently filled with sediments dredged from the adjacent sound. The purpose of this study was to determine if the barrier island terrestrial plant communities were naturally re-establishing through primary succession. In 2006–2008, we compared plant communities, soil carbon and nitrogen, and Aeolian transport of sediments in undisturbed back-dunes, undisturbed shrub thickets, putative back-dunes, and putative shrub thickets. We found that species richness and evenness were low on the filled area relative to adjacent plant communities that had persisted through the storm. Plants on the filled area were almost entirely limited to a band of primarily Spartina patens found at the margin of the sound and there were no signs of establishing the typical zonation of back dune grasses, shrubs, and salt marsh. Evaluation of soil quality suggests that nutrients and organic material are not limiting recovery. Aeolian transport, however, was demonstrably higher across the filled area, where no dense stands of taller plants buffered the airflow. Plant re-establishment is suppressed by wind erosion inhibiting deposition of seeds. Recovery of the site will likely depend on the rhizomatous spread of S. patens from the sound shore. S. patens can then potentially facilitate the colonization of other species by buffering the wind and trapping seeds of other plants. Ironically, this slow recovery may benefit federally threatened bird species that require sparse vegetation for nesting success.     

Effects of feed-supplementation and hide-spray application of two sources of tannins on enteric and hide bacteria of feedlot cattle

Pathogenic bacteria attached to the hide or shed in the feces of cattle at slaughter can contaminate carcasses intended to be processed for human consumption. Therefore, new pre-harvest interventions are needed to prevent the carriage and excretion of foodborne pathogens in cattle presented to the processing plant. The objectives of this study were to examine the antimicrobial effects of hydrolysable tannin-rich chestnut and condensed tannin-rich mimosa extracts on bacterial indicators of foodborne pathogens when applied as a hide-intervention and as a feed additive to feedlot cattle. Water (control) or solutions (3 % wt/vol) of chestnut- and mimosa-extract treatments were sprayed (25 mL) at the left costal side of each animal to a 1000 cm² area, divided in four equal quadrants. Hide-swabs samples obtained at pre-, 2-min, 8-h, and 24-h post-spray application were cultured to enumerate Escherichia coli/total coliforms and total aerobic plate counts. In a second experiment, diets supplemented without (controls) or with (1.5 % of diet dry matter) chestnut- or mimosa-extracts were fed during a 42–day experimental feeding period. Weekly fecal samples starting on day 0, and rumen fluid obtained on days 0, 7, 21 or 42 were cultured to enumerate E.coli/total coliforms and Campylobacter. Tannin spray application showed no effect of treatment or post-application-time (P> 0.05) on measured bacterial populations, averaging 1.7/1.8, 1.5/1.6 and 1.5/1.7 (log₁₀ CFU/cm²) for E. coli/total coliforms, and 4.0, 3.4 and 4.2 (log₁₀CFU/cm²) in total aerobes for control, chestnut and mimosa treatments, respectively. Mean (± SEM) ruminal E. coli and total coliform concentrations (log₁₀ CFU/mL) were reduced (P< 0.01) in steers fed chestnut-tannins (3.6 and 3.8 ± 0.1) in comparison with the controls (4.1 and 4.2 ± 0.1). Fecal E. coli concentrations were affected by treatment (P< 0.01), showing the highest values (log₁₀ CFU/g) in fecal contents from mimosa-fed steers compared to controls (5.9 versus 5.6 ± 0.1 SEM, respectively). Total coliforms (log CFU/g) showed the highest values (P< 0.01) in feces from chestnut- and mimosa-fed steers (6.0 and 6.1 ± 0.1 respectively) in comparison with controls (5.7 ± 0.1). Fecal Campylobacter concentrations (log₁₀CFU/g) were affected by treatment (P< 0.05), day (P< 0.001) and their interaction (P< 0.01) with the controls having lower concentrations than chestnut- and mimosa-fed steers (0.4, 1.0, and 0.8 ± 0.3, respectively). It was concluded that under our research conditions, tannins were not effective in decreasing measured bacterial populations on beef cattle hides. Additionally, chestnut tannin reduced E. coli and total coliforms within the rumen but the antimicrobial effect was not maintained in the lower gastrointestinal tract. Further research is necessary to elucidate the possible antimicrobial effects of tannins at site-specific locations of the gastrointestinal tract in beef cattle fed high-grain and high-forage diets.     

ESTIMATED RUNOFF FROM MAN-MADE SNOW1

ABSTRACT: The consumptive loss from man-made snowmaking at six Colorado ski areas is calculated. The focus of the procedures in this investigation is on the consumptive loss that occurs to man-made snow particles during the period they reside on or in the snowpack until spring snowmelt (termed the watershed loss). Calculated watershed losses under a variety of precipitation and temperature conditions at six ski areas varied from 7 to 33 percent. These calculations were made using the calibrated Subalpine Water Balance Simulation Model (Leaf and Brink, 1973a, 1973b). The watershed loss of 7 to 33 percent indicates the range of likely watershed losses that can be expected at Colorado ski areas. A previous paper by the authors (Eisel et al., 1988) provided estimates of the mean consumptive loss during the snowmaking process (termed initial loss) for conditions existing at Colorado ski areas to be 6 percent of water applied. Therefore, based on the mean initial loss, the total consumptive loss from man-made snowmaking under conditions found at Colorado ski areas could be expected to range from 13 to 37 percent. These results demonstrate the range of total consumptive losses that could be expected in various years and for various watershed conditions. These total percentage losses cannot be extrapolated directly to other specific sites because the total consumptive loss is dependent on temperature during actual snowmaking, temperature and precipitation throughout the winter at the specific ski area, and watershed conditions at the ski area. Consumptive losses to man-made snow for a specific ski area should be estimated using the handbook procedures developed especially for this purpose (Colorado Ski Country USA, 1986b).     

Productivity equation for reclaiming surface mines

This article addresses the development of an agricultural productivity equation for predicting new soil (neo-sol) plant growth potential in Clay County, Minnesota, USA. Soil factors examined in the study include percent organic matter, percent slope, percent rock fragments, hydraulic conductivity, electrical conductivity, pH, topographic position, available water-holding capacity, bulk density, and percent clay. Squared terms and two-factor interaction terms were also examined as possible regressors. A best equation was selected that had a multiple coefficient of determination of 0.7399 and has five significant regressors and intercept withP.0001. The regressors are hydraulic conductivity, percent slope squared, bulk density times percent rock fragments, electrical conductivity times percent rock fragments, and electrical conductivity times percent organic matter. The regressors predict soil suitability for a general crop model. The crops included in the model are wheat, oats, barley, soybeans, sugar beets, sunflowers, and grasses/legumes.     

World diamond industry: 1970–2000

The diamond cartel has remained effective in controlling diamond supplies and prices for a longer period than any other major minerals cartel in the twentieth century. This paper examines the diamond pipeline from mine to the jewellery market. Particular attention is given to the characteristics of diamonds and of the industry structure that make the long-term stability of the cartel possible. The strategies of De Beers Consolidated Diamond Mines in controlling rough gem supplies and prices are examined, and projections are made to 2000. The paper shows that in spite of a projected decrease in the share of total diamond production sold through the De Beers' cartel in the 1990s, the cartel is expected to continue effectively to control supplies and prices.