Transport of coliphage PRD1 in a surface flow constructed wetland.

A tracer study was conducted in a 3-ha surface flow constructed wetland to analyze transport performance of PRD1, an enteric virus model. The convection-dispersion equation (CDE), including a first-order reaction model, adequately simulated transport performance of PRD1 in the wetland under an average hydraulic loading rate of 82 mm/d. Convective velocity (v) and longitudinal dispersion coefficient (D) were estimated by modeling a conservative tracer (bromide) pulse through the wetland. Both PRD1 and bromide were simultaneously added to the entering secondary treated wastewater effluent. The mass of bromide and PRD1 recovered was 76 and 16%, respectively. The PRD1 decay rate was calculated to be 0.3/day. The findings of this study suggest that the CDE model and analytical moment equations represent a suitable option to characterize virus transport performance in surface flow constructed wetlands.     

Nuclear waste disposition and site remediation: U.S. department of energy,state governments,and other stakeholders dialogue

Remediation responsibilities of the U.S. Department of Energy (DOE) encompass a vast national complex of highly contaminated former weapons facilities. During the mid‐1990s, DOE announced its intentions to consolidate some waste types at specific sites. At about the same time, organizations and public officials around DOE sites urged a National Dialogue, designed to develop comprehensive solutions to the Department's needs for waste disposition ( transportation, treatment, and storage). Recent opposition from citizens and elected officials in Nevada and Washington State has presented obstacles to DOE's plans. Additionally, chairs of nine site‐specific advisory boards recommended that DOE support a National Stakeholder Forum, similarly designed to develop solutions to disposition needs. This article reviews the chronology of DOE's disposition efforts, along with public and state reactions and recommendations. © 2006 Wiley Periodicals, Inc.     

The DNA damage activity (DDA) assay and its application to river waters and diesel exhausts

An extremely sensitive assay has been developed for DNA-damaging chemicals using a DNA repair-deficient strain of Escherichia coli. Considerable DNA-damaging activity has been demonstrated by this assay in natural surface-water samples and in diesel-exhaust particulate extracts.     

In situ uranium stabilization by microbial metabolites

Microbial melanin production by autochthonous bacteria was explored in this study as a means to increase U immobilization in U contaminated soil. This article demonstrates the application of bacterial physiology and soil ecology for enhanced U immobilization in order to develop an in situ, U bio-immobilization technology. We have demonstrated microbial production of a metal chelating biopolymer, pyomelanin, in U contaminated soil from the Tims Branch area of the Department of Energy (DOE), Savannah River Site (SRS), South Carolina, as a result of tyrosine amendments. Bacterial densities of pyomelanin producers were >10(6) cells per g wet soil. Pyomelanin demonstrated U complexing and mineral binding capacities at pH 4 and 7. In laboratory studies, in the presence of goethite or illite, pyomelanin enhanced U sequestration by these minerals. Tyrosine amended soils in a field test demonstrated increased U sequestration capacity following pyomelanin production up to 13 months after tyrosine treatments.     

Assessment of physically-based and data-driven models to predict microbial water quality in open channels

In the present study,a physically-based hydraulic modeling tool and a data-driven approach using artificial neural networks (ANNs) were evaluated for their ability to simulate the fate and transport of microorganisms in a water system.To produce reliable data,a pipe network was constructed and a series of experiments using a fecal coliform indicator (Escherichia coli 15597) was conducted.For the physically-based model,morphological (pipe size,link length,slope,etc.) and hydraulic (flow rate) conditions were used as input variables,and for ANNs,water quality parameters (conductivity,pH,and turbidity) were used.Both approaches accurately described the fate and transport of microorganisms (physically-based model: correlation coefficient (R) in the range of 0.914 – 0.977 and ANNs: R in the range of 0.949 – 0.980),with the exception of one case at a low flow rate (q = 31.56 cm 3 /sec).This study also indicated that these approaches could be complementarily utilized to assess the vulnerability of water facilities and to establish emergency plans based on hypothetical scenarios.