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151.
Kraaijeveld K Reumer BM Mouton L Kremer N Vavre F van Alphen JJ 《Die Naturwissenschaften》2011,98(3):175-180
Wolbachia is a maternally inherited bacterium that manipulates the reproduction of its host. Recent studies have shown that male-killing
strains can induce cytoplasmic incompatibility (CI) when introgressed into a resistant host. Phylogenetic studies suggest
that transitions between CI and other Wolbachia phenotypes have also occurred frequently, raising the possibility that latent CI may be widespread among Wolbachia. Here, we investigate whether a parthenogenesis-inducing Wolbachia strain can also induce CI. Parthenogenetic females of the parasitoid wasp Asobara japonica regularly produce a small number of males that may be either infected or not. Uninfected males were further obtained through
removal of the Wolbachia using antibiotics and from a naturally uninfected strain. Uninfected females that had mated with infected males produced
a slightly, but significantly more male-biased sex ratio than uninfected females that had mated with uninfected males. This
effect was strongest in females that mated with males that had a relatively high Wolbachia titer. Quantitative PCR indicated that infected males did not show higher ratios of nuclear versus mitochondrial DNA content.
Wolbachia therefore does not cause diploidization of cells in infected males. While these results are consistent with CI, other alternatives
such as production of abnormal sperm by infected males cannot be completely ruled out. Overall, the effect was very small
(9%), suggesting that if CI is involved it may have degenerated through the accumulation of mutations. 相似文献
152.
OBJECTIVE: A critical review of the existing evaluation studies on the Drug Evaluation and Classification (DEC) program was conducted to determine the validity and accuracy of the technique for identifying drivers under the influence of drugs. METHODS: Studies were divided into two categories--laboratory studies and field (i.e., enforcement) studies. A classification process was devised using common criteria based on the toxicology findings (i.e., drug positive or drug negative) and the opinion of the police officer who assessed the driver (i.e., drug positive or drug negative). A series of standard measures (Sensitivity, Specificity, False Alarm Rate, Miss Rate, Corroboration, and Accuracy) were calculated for each to assess the effectiveness of the DEC program. RESULTS: Laboratory studies do not provide overwhelming support for the accuracy with which officers trained in the DEC program can detect and identify the particular class(es) of drug involved based on psychophysical assessment alone. The detection and identification of the relatively low levels of drugs administered were typically better than chance but many cases were missed. The fact that some drugs were detected with greater accuracy than others suggests that the effects of these substances were more prominently manifested in the symptomology assessed by the DEC procedure. Although field enforcement studies are not as scientifically rigorous as laboratory studies, DEC assessments in an enforcement context have the benefit of information obtained from the arresting officer and from interviews with the suspect. In addition, the drug doses consumed by users are typically much higher than those permitted in controlled laboratory studies. In general, officers trained in the DEC program are able to identify persons under the influence of drugs and to specify the drug class responsible with a degree of accuracy that not only exceeds chance, but in some cases reaches a very high level. CONCLUSIONS: There remains room for improvement in the DEC program. As further research becomes available, either from laboratory or field investigations or both, it needs to be incorporated into the program to enhance its validity and accuracy. 相似文献
153.
The elemental composition of solutes transported by rivers reflects combined influences of surrounding watersheds and transformations within stream networks, yet comparatively little is known about downstream changes in effects of watershed loading vs. in-channel processes. In the forested watershed of a river under a mediterranean hydrologic regime, we examined the influence of longitudinal changes in environmental conditions on water-column nutrient composition during summer base flow across a network of sites ranging from strongly heterotrophic headwater streams to larger, more autotrophic sites downstream. Small streams (0.1-10 km2 watershed area) had longitudinally similar nutrient concentration and composition with low (approximately 2) dissolved nitrogen (N) to phosphorus (P) ratios. Abrupt deviations from this pattern were observed in larger streams with watershed areas > 100 km2 where insolation and algal abundance and production rapidly increased. Downstream, phosphorus and silica concentrations decreased by > 50% compared to headwater streams, and dissolved organic carbon and nitrogen increased by approximately 3-6 times. Decreasing dissolved P and increasing dissolved N raised stream-water N:P to 46 at the most downstream sites, suggesting a transition from N limitation in headwaters to potential P limitation in larger channels. We hypothesize that these changes were mediated by increasing algal photosynthesis and N fixation by benthic algal assemblages, which, in response to increasing light availability, strongly altered stream-water nutrient concentration and stoichiometry in larger streams and rivers. 相似文献
154.
The aim of this paper is to tackle the problem that arises from asymmetrical data cubes formed by two crossed factors fixed by the experimenter (factor A and factor B, e.g., sites and dates) and a factor which is not controlled for (the species). The entries of this cube are densities in species. We approach this kind of data by the comparison of patterns, that is to say by analyzing first the effect of factor B on the species-factor A pattern, and second the effect of factor A on the species-factor B pattern. The analysis of patterns instead of individual responses requires a correspondence analysis. We use a method we call Foucart's correspondence analysis to coordinate the correspondence analyses of several independent matrices of species x factor A (respectively B) type, corresponding to each modality of factor B (respectively A). Such coordination makes it possible to evaluate the effect of factor B (respectively A) on the species-factor A (respectively B) pattern. The results obtained by such a procedure are much more insightful than those resulting from a classical single correspondence analysis applied to the global matrix that is obtained by simply unrolling the data cube, juxtaposing for example the individual species x factor A matrices through modalities of factor B. This is because a single global correspondence analysis combines three effects of factors in a way that cannot be determined from factorial maps (factor A, factor B, and factor A x factor B interaction) whereas the applications of Foucart's correspondence analysis clearly discriminate two different issues. Using two data sets, we illustrate that this technique proves to be particularly powerful in the analyses of ecological convergence which include several distinct data sets and in the analyses of spatiotemporal variations of species distributions. 相似文献
155.
Souissi Imen Boisson Jean Marie Mekki Insaf Therond Olivier Flichman Guillermo Wery Jacques Belhouchette Hatem 《Regional Environmental Change》2018,18(3):637-650
Regional Environmental Change - This study considers a quantitative approach for assessing the performance of Tunisian farming systems to face climate change. It is based on the resilience concept... 相似文献
156.
Marie-Eve Dubuis Hamza M’Bareche Marc Veillette Bouchra Bakhiyi Joseph Zayed Jacques Lavoie 《Journal of the Air & Waste Management Association (1995)》2017,67(11):1258-1271
This study sought to fill the gap in information about the type and the concentration of bioaerosols present in the air of biomethanization facilities (BF). Evaluation of bioaerosol composition and concentration was achieved in two biomethanization facilities located in Eastern Canada, during summer and winter. In order to have a thorough understanding of the studied environment, the methodology combined culture of bacteria and molds, qualitiative polymerase chain reaction (qPCR) for specific microorganisms, endotoxin quantification, and next-generation sequencing (NGS) for bacterial diversity. Results revealed that workers in biomethanization facilities are exposed to bioaerosols and pathogenic microorganisms similar to those found in composting plants. However, human exposure levels to bioaerosols are lower in BF than in composting plants. Despite these differences, use of personal protective equipment is recommended to lower the risks of health problems.
Implications: Biomethanization is a new technology used in eastern Canada for waste management. In the next few years, it is expected that there will be an expansion of facilities in response of tight governmental regulations. Workers in biomethanization facilities are exposed to various amounts of bioaerosols composed of some harmful microorganisms. Therefore, monitoring this occupational exposure could be an interesting tool for improving worker’s health. 相似文献
157.
A Spatially Explicit Resource-Based Approach for Managing Stream Fishes in Riverscapes 总被引:1,自引:0,他引:1
The article describes a riverscape approach based on landscape ecology concepts, which aims at studying the multiscale relationships
between the spatial pattern of stream fish habitat patches and processes depending on fish movements. A review of the literature
shows that few operational methods are available to study this relationship due to multiple methodological and practical challenges
inherent to underwater environments. We illustrated the approach with literature data on a cyprinid species (Barbus barbus) and an actual riverscape of the Seine River, France. We represented the underwater environment of fishes for different discharges
using two-dimensional geographic information system-based maps of the resource habitat patches, defined according to activities
(feeding, resting, and spawning). To quantify spatial patterns at nested levels (resource habitat patch, daily activities
area, subpopulation area), we calculated their composition, configuration, complementation, and connectivity with multiple
spatial analysis methods: patch metrics, moving-window analysis, and least cost modeling. The proximity index allowed us to
evaluate habitat patches of relatively great value, depending on their spatial context, which contributes to the setting of
preservation policies. The methods presented to delimit potential daily activities areas and subpopulation areas showed the
potential gaps in the biological connectivity of the reach. These methods provided some space for action in restoration schemes. 相似文献
158.
159.
This paper presents an application of the ELECTRE III decision-aid method in the context of choosing a sustainable demolition waste management strategy for a case study in the city of Lyon, France. This choice of waste management strategy takes into consideration the sustainable development objectives, i.e. economic aspects, environmental consequences, and social issues. Nine alternatives for demolition waste management were compared with the aid of eight criteria, taking into account energy consumption, depletion of abiotic resources, global warming, dispersion of dangerous substances in the environment, economic activity, employment, and quality of life of the local population. The case study concerned the demolition of 25 buildings of an old military camp. Each alternative was illustrated with different waste treatments, such as material recovery, recycling, landfilling, and energy recovery. The recommended solution for sustainable demolition waste management for the case study is a selective deconstruction of each building with local material recovery in road engineering of inert wastes, local energy recovery of wood wastes, and specific treatments for hazardous wastes. 相似文献
160.
The radiation protection community has only recently started the important work of preparedness for long-term post-accidental management of radioactively contaminated areas, like for instance the EC projects STRATEGY, FARMING and EURANOS and the French authorities' CODIRPA and PAREX programmes. There are, however, different views concerning how long a long-term management might last. Based on the Norwegian and former Soviet Union experience after the Chernobyl accident, it is clear that a nuclear accident can entail decades of necessary management and rehabilitation of living conditions. The time period is dependent on a number of factors, e.g. amount of fallout, type of radionuclides, land use of contaminated area, number and density of people affected and available techniques and resources for implementing countermeasures. This paper discusses the management strategy implemented in Norway after the Chernobyl accident, the need for changing strategy over time and the important involvement of affected groups. Careful planning and reflections should be undertaken before actions are taken in the recovery phase, keeping in mind the possibility of decades with problems. 相似文献