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861.
Amniocentesis performed at the 12th week and later gives reliable results. The procedure can be performed using regimens developed for mid-trimester amniotic fluid (AF) cells. Extension to the 10th–11th week is, in principle, feasible. However, the high cytogenetic failure rate is a difficulty and despite a high clone count, the culture time is prolonged. The problem of the relatively high loss of AF could be overcome by cell filtration techniques and replacement of the fluid. Because of the short turnover rate of the AF, this may be unnecessary or replacement with an isotonic solution may be sufficient. (Pseudo)mosaicism appears to occur more frequently in early than in late amniocentesis. As yet, data are too sparse to allow a comparison with chorionic villus sampling. There are no reliable follow-up data from which to estimate the abortion rate and the number of embryonic malformations. 相似文献
862.
For pregnancies less than 17 menstrual weeks, increasing amounts of nuchal fluid increase the risks of chromosome abnormalities with localized nuchal fluid, diffuse nuchal fluid, cystic hygroma, and fetal hydrops having chromosomal risks of 12, 23, 50, and 78 per cent, respectively. The ultrasound appearance of localized or diffuse nuchal fluid is not a specific discriminator, but a fluid depth of greater than or equal to 5 mm may be an indicator of increased risk of fetal chromosomal abnormalities. If the fluid depth is less than 5 mm, there is a stronger negative predictive value and negative likelihood risk of a fetal chromosome abnormality. Gestational age did not improve the fluid depth predictive value. Differentiation of physiological from pathological requires chromosome analysis, serial ultrasound evaluation, and good clinical examination as a newborn and possibly as a young child. Long-term follow-up of those cases identified with resolving nuchal fluid abnormalities is not available and is required for a complete understanding of physiological and pathological aetiologies. Genetic counselling for fetal nuchal fluid would be recommended. 相似文献
863.
Dr. J. A. M. A. Tan J. S. H. Tay L. I. Lin S. K. Y. Kham J. N. Chia T. M. Chin Norkamar Bt. Adb. Aziz H. B. Wong 《黑龙江环境通报》1994,14(11):1077-1082
β-Thalassaemia major patients have chronic anaemia and since 3–4 per cent of Singaporeans carry the β-gene, prenatal diagnosis is essential. We evaluated the amplification refractory mutation system (ARMS) technique as a routine test for prenatal diagnosis of β-major. Six mutations along the β-gene were studied—41–42 (-TCTT), IVSII #654 (C-T), 17β (A-T), – 28 TATA (A-G), IVSI #5 (G-C), and IVSI #1 (G-T). Our results indicate that prenatal diagnosis using these mutations can be offered to 90 per cent (35/39) of our Chinese couples and 54·6 per cent (12/22) of our Malay couples at risk. Confirmation of ARMS results was carried out using allele-specific oligonucleotide hybridization. Prenatal diagnosis using ARMS was successfully carried out in nine cases which included a set of triplets and twins. The triplets were diagnosed with the β-trait carrying the 41–42 mutation. The couple with twins possessed the #654 mutation and one twin was diagnosed with the β-trait and the other with #654 homozygosity. Genomic sequencing of the undefined mutations in the Chinese couples revealed rarer mutations at − 29 and an ATG-AGG base substitution at the initiation codon for translation. In the Malay couples, genomic sequencing detected mutations at codon 15 (TGG-TAG) and codon 26 (GAG-AAG). We conclude that ARMS with its direct detection of amplified products by gel electrophoresis provides an accurate, rapid, and simpler method for our β-thalassaemia prenatal diagnosis programme in Singapore. 相似文献
864.
Experience indicates that the most likely explanation for a mixture of 46,XX/46,XY cells in an amniotic fluid sample is that of maternal cell contamination and that a normal male child is to be expected at birth. We report the birth of a normal female child following prenatal diagnosis of such a mixture. Extensive postnatal studies failed to reveal an XY cell line. The possible sources of the XY cell line are discussed, as are the various techniques that were applied in an effort to discover it's origin. Cross-contamination of samples could be ruled out and there was no evidence of an unsuspected twin pregnancy. It is clear from this case that not all 46,XX/46,XY results obtained in amniotic fluid can be assumed to represent maternal cell contamination and some effort should be made to eliminate other potential sources for such a mixture. 相似文献
865.
866.
Dr. Elizabeth J. T. Winsor B. St. John Brown Edwin R. Luther Stephen A. Heifetz J. Philip Welch 《黑龙江环境通报》1987,7(7):485-489
A pregnancy was terminated because of persistently elevated amniotic fluid AFP (+10 S.D.) and an AChE band of low intensity on gel electrophoresis. No fetal anomalies were detected by ultrasonographic examination. Autopsy revealed an apparently normal fetus of about 20 weeks gestation. Attached to the placenta was a small sac containing a fetus papyraceus co-twin of about 8–9 weeks gestation. The small deceased co-twin and its gestational sac were not detected prenatally despite multiple ultrasonographic examinations. The difficulty in the interpretation of apparently conflicting results is emphasized. 相似文献
867.
In 226 women requesting chorionic villus sampling (CVS), routine cervical cultures were obtained before the procedure. Transcervical CVS was performed irrespective of the test results. The prevalence of potential pathogens in cervical cultures at our institution is low. Beta haemolytic Streptococcus was cultured in 3 per cent of the women. No pathogenic microorganisms were isolated in 64 per cent of the women. There was no relationship between culture results and the outcome of pregnancy. These observations suggest that adequate antiseptic cleansing of the genital tract is a suitable approach and there is no need to routinely perform cultures before CVS. 相似文献
868.
Dr Sara Ahmed 《The Environmentalist》1994,14(1):3-16
Summary People's participation is usually regarded as a sine qua non for the success and sustainability of development projects. Yet in practice, it raises a number of questions. Who are the people? Why is their participation sought, and how or at what level, is such participation desired? This paper seeks to examine the rhetoric of participation in the implementation of the Ganga Action Plan (GAP) at Varanasi, in the north-eastern State of Uttar Pradesh, India. Launched in 1985, the GAP is the first major attempt to systematically control and monitor the pollution of a significant river in the country. In addition, it claimed to be a people's programme because of the powerful and deep-seated cultural and religious meaning associated with the Ganga. Varanasi, however, is indicative of its failure to deliver this promise — the GAP is only acceptable to authority because it does not challenge the existing institutional order, and its participatory content is symbolic rather than substantive. Non-govemental organisations, traditionally viewed as intermediary actors between the micro and macro levels, work within the socio-political framework of the city. In the process, water-user groups such as the washermen who derive an economic livelihood from washing clothes in the Ganga, are literally excluded from the definition and process of participation.She obtained her PhD, the basis of the current research, at the University of Cambridge, UK. 相似文献
869.
The role of light in induction of sexual maturation in male Octopus vulgaris has been studied on experimentals reared in the laboratory. Experiments were carried out during January, February and March, the time of the year when females do not breed or are just beginning to breed. Males (20 to 500 g) were artificially illuminated for 8 or 16 h/day or kept in complete darkness. Their gonads were compared with those of wild specimens killed a few hours after capture. In the Mediterranean Sea, under natural conditions, O. vulgaris males of less than 150 g never display sexual maturity; under our experimental conditions, young males from 70 g onwards, illuminated 16 h daily, show gonad development. Males of over 150 g, illuminated 16 h daily, have a larger number of spermatophores than wild specimens of individuals illuminated 8 h daily. O. vulgaris reared in complete darkness are sexually more mature than wild individuals. An hypothesis on induction of sexual maturation in the Cephalopoda is proposed. 相似文献
870.
Five hundred cordocenteses were performed between 12 and 21 weeks. The indications were thalassaemia (386), rapid karyotyping (97), feto-maternal allo-immunization (10), rubella (6), and toxoplasmosis (1). One hundred and ten pregnancies underwent termination on the basis of the result, while 20 of the 370 pregnancies intended to continue were lost to follow-up. Amongst these were 16 fetal losses (4·3 per cent) and 22 premature deliveries (5·9 per cent); no other complications were reported. Four adverse prognostic factors were identified: (a) cord bleeding; (b) fetal bradycardia; (c) prolonged procedure time; and (d) anterior insertion of the placenta. There was no‘obvious’ difference in fetal loss rate with advancing gestation until 19–21 weeks, when the risk of fetal loss decreased to 2·5 per cent. 相似文献