Low paternity in the hornet Vespa crabro indicates that multiple mating by queens is derived in vespine wasps

Queen mating frequency was studied in the European hornet, Vespa crabro, by analyzing four DNA microsatellite loci in 20 workers from each of 14 nests. Queens were found to be predominantly singly mated (9/14), although double (4/14) and triple mating (1/14) also occurred. For most multiply mated queens, paternity was significantly biased with the majority male fathering on average 80% of the female offspring. The population-wide effective mating frequency was therefore low (1.11), and sister-sister relatedness high (0.701 ± 0.023 SE). Low effective mating frequency in Vespa, in combination with data from other vespines, suggests that high paternity frequency is derived in the group. Some problems with the non-detection of fathers, where the queen was not sampled or shared alleles with males, are analyzed. Received: 16 November 1998 / Received in revised form: 29 March 1999 / Accepted: 12 April 1999     

Metabolic transformations of acquired lucibufagins by firefly “femmes fatales”

Summary. Female Photuris fireflies sequester defensive steroidal pyrones (lucibufagins) from male fireflies of the genus Photinus. Lucibufagin analyses of Photuris females and Photinus ignitus males show that the lucibufagin mixtures of predator and prey differ in their composition. Analyses of whole body extracts showed that P. ignitus males contain a mixture of eight non-glycosylated lucibufagins, composed mostly of compounds with two oxygenated positions in the steroidal A-ring (C-3, C-5). After feeding on P. ignitus males, Photuris females contain six major lucibufagins. Three of these compounds are not present in the prey, including the novel lucibufagin glycoside 5β,11α-dihydroxy-12-oxo-3β-O-β-D-xylopyranosylbufalin, and two other lucibufagins with a trioxygenated A-ring (C-2, C-3, C-5). These results indicate that Photuris females transform the sequestered lucibufagins both by glycosylation and oxidation, which could affect the systemic transportability of these compounds due to an increase in their polarity. Received 18 February 1999; accepted 19 April 1999.     

Chemical egg defense in Photuris firefly “femmes fatales”

Summary. Female Photuris fireflies contain defensive chemicals of two types. They sequester steroidal pyrones (lucibufagins) from male fireflies of the genus Photinus that they eat, and themselves produce the defensive betaine N-methylquinolinium 2-carboxylate. Chemical analyses of Photuris eggs showed that females that fed on Photinus males endow their eggs with both lucibufagin and the betaine, while those that did not feed on Photinus lay eggs that contain betaine, but virtually no lucibufagin. Photuris females collected in the field during the Photinus flight season laid eggs that invariably contained betaine, but lucibufagin only at times. Predation experiments showed that Photuris eggs are essentially unacceptable to larvae of a coccinellid beetle (Harmonia axyridis) and an ant (Leptothorax longispinosus), but moderately acceptable to an earwig (Forficula auricularia). When applied experimentally to palatable insect eggs, lucibufagin proved deterrent to these three predators, while the betaine proved deterrent to the ant and coccinellid larva only. Both types of defensive compound decreased egg predation in the field. By endowing their eggs with both exogenous and endogenous chemicals, Photuris females are essentially “maximizing their options”– when feeding on Photinus, their eggs are doubly protected, but they are not entirely defenseless when the females are unable to procure lucibufagin. Received 12 August 1999; accepted 27 August 1999     

Measurements of selected PCBs in open urban ambient air of Madrid (Spain)

The focus of this study was to characterize the concentration levels of selected PCBs and compare them to compiled data in order to contribute to the international database. The sampling site is located in the outskirts of Madrid and can be considered an open urban area. 32 samples of air were taken from February 1998 to June 1998 by using a high volume air sampler. Glass fiber filters and polyurethane foam (PUF) were used to collect the paniculate and gas phase material, respectively. PUF plugs were Soxhlet extracted and filters were ultrasonically extracted by using pesticide-grade hexane and dichloromethane, respectively. The cleanup procedure was carried out on a florisil column with hexane and hexane/dichloromethane as elution solvents. GC/MS in a selected ion monitoring mode was used for quantification and 29 selected PCBs congeners were analyzed.     

Validating graph-based connectivity models with independent presence–absence and genetic data sets

Habitat connectivity is a key objective of current conservation policies and is commonly modeled by landscape graphs (i.e., sets of habitat patches [nodes] connected by potential dispersal paths [links]). These graphs are often built based on expert opinion or species distribution models (SDMs) and therefore lack empirical validation from data more closely reflecting functional connectivity. Accordingly, we tested whether landscape graphs reflect how habitat connectivity influences gene flow, which is one of the main ecoevolutionary processes. To that purpose, we modeled the habitat network of a forest bird (plumbeous warbler [Setophaga plumbea]) on Guadeloupe with graphs based on expert opinion, Jacobs’ specialization indices, and an SDM. We used genetic data (712 birds from 27 populations) to compute local genetic indices and pairwise genetic distances. Finally, we assessed the relationships between genetic distances or indices and cost distances or connectivity metrics with maximum-likelihood population-effects distance models and Spearman correlations between metrics. Overall, the landscape graphs reliably reflected the influence of connectivity on population genetic structure; validation R² was up to 0.30 and correlation coefficients were up to 0.71. Yet, the relationship among graph ecological relevance, data requirements, and construction and analysis methods was not straightforward because the graph based on the most complex construction method (species distribution modeling) sometimes had less ecological relevance than the others. Cross-validation methods and sensitivity analyzes allowed us to make the advantages and limitations of each construction method spatially explicit. We confirmed the relevance of landscape graphs for conservation modeling but recommend a case-specific consideration of the cost-effectiveness of their construction methods. We hope the replication of independent validation approaches across species and landscapes will strengthen the ecological relevance of connectivity models.     

The regional emergency plan requirement: Application of the best practices to the Brazilian case

The objective of this study is to define new requirements for a Brazilian regional emergency plan based on the best practices used in countries such as the USA, UK, Canada, Japan and Australia. Therefore, the risk analysis methodologies and emergency frameworks used in these countries will be taken into account and a critical analysis will be carried out in order to customize and apply their best practices to Brazilian case. In addition, other issues will be looked at, such as the number of accidents and environmental impact in some areas, related to environmental sensitivity in order to define the environment reliability of the whole system in a specific area. Currently in Brazil resources are not optimized in an emergency offshore plan, rather company has to implement its own emergency plan without any kind of coordination. Despite the existence of some procedures, there are no national or regional frameworks to coordinate emergency plans in Brazil.     

Transformational and transactional leadership and innovative behavior: The moderating role of psychological empowerment

Innovative behavior is increasingly important for organizations' survival. Transformational leadership, in contrast to transactional leadership, has been argued to be particularly effective in engendering follower innovative behavior. However, empirical evidence for this relationship is scarce and inconsistent. Addressing this issue, we propose that follower psychological empowerment moderates the relationship of transformational and transactional leadership with follower innovative behavior. In a field study with 230 employees of a government agency in the Netherlands combining multisource ratings, we show that transformational leadership is positively related to innovative behavior only when psychological empowerment is high, whereas transactional leadership has a negative relationship with innovative behavior only under these conditions. Copyright © 2009 John Wiley & Sons, Ltd.     

Failure rate evaluation with influencing factors

A new methodology for failure rate evaluation with influencing factors is proposed. A quantitative part allows integrating potential available data from feedback. Then, a qualitative analysis deals with influencing factors such as design, environment, and use to provide more coherent and argued results. The main idea is to use some criteria to fix the failure rate within a prior interval, according to the influencing factor states. To this end, the system is broken up into main component groups. When a component group is susceptible to an influencing factor, its baseline failure rate is multiplied by the relevant influencing coefficient. A seven-step methodology is presented to define the model, identify and rate the influencing factors, set indicator functions taking the uncertainties into account, and calculate the influencing coefficients. The proposed methodology is global enough to be usable for a large number of safety instrumented systems and influencing factors. For example, an application regarding safety pressure relief valves is given. The prospect is for more efficient risk management by acting both on systems and influencing factors.     

Process chemicals – An ever present concern during plant shutdowns

Chemical accidents during process plant shutdowns may have severe consequences. In spite of this, the safety management systems in place in many companies still mainly cover the normal operations and little explicitly addresses the maintenance shutdown and plant start-up phases. A Finnish research project found that the level of safety during shutdowns is more closely related to the skills of key individuals at the plants and less due to the systematic safety management system of the companies. A guidebook on the topic in conjunction with tools to improve the situation were developed during the project in order to help the companies improve their documentation associated with chemical safety during shutdowns.     

Estimation of chemical concentration due to fugitive emissions during chemical process design

Fugitive emissions are not an environmental concern alone, but are also a health concern. From occupational health standpoint, fugitive emissions are the main sources of origin of the continuous exposure to workers. Operating plants regularly measure release and concentration levels through a plant-monitoring program. However, for processes which are still ‘on paper’, predictive estimation methods are required. Therefore, three methods for estimating concentration of the fugitive emissions are presented for the process development and design phases of petrochemical processes. The methods estimate the fugitive emission rates and plant plot dimensions resulting to fugitive emission concentrations. The methods were developed for the type and amount of information available in three process design stages; conceptual design, preliminary process design, and detailed process design. The methods are applied on a real benzene plant; the estimated benzene concentrations are compared to the actual concentration measured at the plant. The results show that as the information mounts up during design, the concentration estimate becomes more accurate. The results indicate that the methods presented provide simple estimates of fugitive emission-based concentrations during the design stages.