Baseline element composition of foliose and fruticose lichens along the steep climatic gradient of SW Patagonia (Aisén Region, Chile)

Samples of foliose (Nephroma antarcticum) and fruticose (Usnea sp.) lichens were collected across a steep climatic and vegetation gradient in a remote, almost pristine region of SW Chilean Patagonia. Concentrations of major and trace elements in lichens from the rainforest were among the lowest ever reported worldwide for foliose and fruticose lichens and can be considered background levels for the region. The two lichen growth forms showed different elemental compositions mainly due to the greater capacity of foliose thalli to intercept elements from windborne and canopy-leached particles. The patterns of spatial variation in the chemical composition of lichens were effectively explained by statistical methods and reflected the different availability of wet and dry deposition along the steep climatic gradient. Baseline values established for N. antarcticum samples growing in temperate Nothofagus forests were therefore distinct from those of samples growing in more open, drier habitats. The fruticose Usnea sp. showed a higher affinity for atmophile Hg, low concentrations of lithophilic elements, and the same baseline composition whether from temperate forests or from dry, barren environments. The provided background and baseline values against which variations can be measured will be useful in the early detection of local or regional climatic and environmental change, especially in view of the planned construction of hydropower dams under the recently approved HidroAysén Project.     

Groundwater contamination evolution in the Guadiamar and Agrio aquifers after the Aznalcóllar spill: assessment and environmental implications

In 1998, the Agrio and Guadiamar rivers underwent an enormous environmental disaster caused by the rupture of the Aznalcóllar tailings dam and the release of 6 hm³ of pyrite sludge and acidic water. Both rivers run over recent alluvial materials which form a small-sized aquifer which is however important because underground water feeds the flow of the rivers. This work analyzes the state of groundwater 10 years after the spill. Before the dam failure, this aquifer was already contaminated in the zone nearest to the mine, to which the impact of the spill was added. Contamination levels in the alluvial aquifer of the Agrio River have decreased remarkably. However, they are still important, with acidic pH values and high concentrations of toxic elements (maximum values of 16 mg/L of Zn and 15 mg/L of Al). There are also important levels of contamination in the Guadiamar alluvial area closest to the mine, as well as in specific zones located further south. The concentration of toxic elements is mainly controlled by pH. The evolution of contaminant levels show a sharp decrease after the first years following the spill, followed by a subsequent stabilization. It is necessary to take measures for the recovery of the aquifer because, otherwise, groundwater will continue contributing contaminants into the Agrio and Guadiamar rivers.     

Monitoring water quality in reservoirs for human supply through multi-biomarker evaluation in tropical fish

Paraíba do Sul River is located at a very densely inhabited region of Brazil crossing the three most industrialized states of the country (S?o Paulo, Minas Gerais and Rio de Janeiro states). As a result, industrial and farming residues as well as urban sewage are frequently disposed without appropriate treatment. The current study aimed at investigating the water quality in three reservoirs along the Paraíba do Sul River (Ilha dos Pombos, Santa Cecília and Santa Branca), through physiological, morphological, biochemical, and genetic biomarkers. The bioindicator chosen was the catfish Pimelodus maculatus, sampled during the dry (June 2008) and rainy (February 2009) seasons. Also, some water physicochemical parameters were analyzed from the sampling sites, but displayed no alterations according to the Brazilian Agency for Water Quality Legislation. Branchial carbonic anhydrase activity was inhibited in the dry season, while renal carbonic anhydrase activity was inhibited in the rainy season in the Santa Branca reservoir, indicating disturbance of osmoregulatory and acid-base regulation processes. Histopathological alterations were observed in the gills (neoplasic and tissue hyperplasia processes) and liver (necrosis), indicating serious damage to the functional integrity of these organs. A high incidence of melanomacrophage centers was observed in the liver, suggesting an intense immune response in all reservoirs. Acetylcholinesterase and catalase activity showed also differences corroborating some morphological results. Likewise, the induction of the micronucleus and DNA damage indicate genotoxicity, but mainly in the Santa Branca reservoir. Thus, the health status of P. maculatus warrants caution in the use of the water from the 3 reservoirs for direct human consumption, particularly after the accidental spill of endosulfan in November 2008, three months before the rainy season sampling.     

电石渣－铁屑法去除硫酸废水中的氟和砷

对各种处理含氟、砷废水的方法进行了探讨，选择了以电石渣和废铁屑为药剂去除硫酸废水中氟和砷的方法，取得了较好的效果。该法以废治废、工艺简便、运行费用低，处理后的废水可达排放标准。     

Integrating local ecological knowledge and management practices of an isolated semi-arid papyrus swamp (Loboi, Kenya) into a wider conservation framework

The current study documented local utilization of Cyperus papyrus L. (papyrus), harvesting patterns, threats, and local management practices among the Endorois community living around Loboi swamp (Kenya). Papyrus is a highly productive freshwater macrophyte that is widely utilized throughout tropical Africa. However, increased human population and poverty, has led to over exploitation and conversion of papyrus wetlands to agricultural fields. Nonetheless, users of papyrus hold important local ecological knowledge (LEK) and practices. We show that Endorois practices on papyrus uses are compatible with the management priorities of the swamp and a wider conservation framework using data obtained from three focus group discussions (FGD), interviews of 34 households and 15 key informants. The study revealed that papyrus support local livelihood notably as a source of income (papyrus mats are sold), cattle fodder, roofing materials (shelter), and cooking fuel. The study further revealed important LEK relating to harvesting patterns, recovery after harvesting and traditional management practices. Correlation and principal component analyses showed that experienced old harvesters (EXPERT) avoided harvesting repeatedly at the same location (REVISIT), thereby allowing recovery of papyrus when compared to younger harvesters (r = 0.63, p < 0.01). However, over 70% of harvesters predicted a decrease in papyrus coverage in the future due to the current diversion of water from the swamp, and frequent droughts, despite the instituted traditional management strategies (e.g. rotational harvesting) to curb overharvesting. The study concluded that the documentation of site-scale papyrus users' profile, LEK, and traditional practices are vital for the conservation and management of Loboi swamp.     

Evaluation of best management practice products in preventing discharge of metals: a laboratory evaluation

Heavy metal accumulation in soil poses serious environmental and health risks, as metals are carried with eroded soils. In this study, 17 different soil erosion and sediment control products were investigated for their effectiveness in controlling transport of particulate heavy metals (Cu, Zn, Pb, Cd). Among the treatments investigated, wood mulch and tackifiers were found to be the most effective in reducing total suspended solids (TSS) and total heavy metal losses. They reduced TSS to an undetectable level during short-term simulation tests. Paper mulch was the only treatment that had no significant reduction in both total metal loss and TSS. Fiber rolls, silt fences, and gravel bags were effective in reducing sediment loss. Although the netting/blanket treatments were not effective in reducing total metal discharge, they significantly reduced sediment loss compared with the control.     

Finding Space for Participation: Fisherfolk Mobility and Co-Management of Lake Victoria Fisheries

The literature on fisheries co-management is almost silent on the issue of the movement of fisherfolk within fisheries, although such movement must have implications for the effectiveness of co-management. The introduction of co-management often involves the formation of new structures that should enable the participation of key stakeholder groups in decision-making and management, but such participation is challenging for migrating fishers. The article reports on a study on Lake Victoria, East Africa, which investigated the extent of movement around the lake and the implications of movement for how fishers participate and are represented in co-management, and the implications of the extent and nature of movement for co-management structures and processes. The analysis draws on the concept of space from the literature on participation in development and on a framework of representation in fisheries co-management in addressing these questions. The created space is on an 'invited' rather than open basis, reflecting the top-down nature of implementation and the desire to secure participation of different occupational groups, as well as women in a male-dominated sector. The more powerful boat owners dominate positions of power within the co-management system, particularly as the levels of co-management, from sub-district to national, are traversed. The limited power and resources of boat crew are exacerbated by the degree and nature of movement around the lake, making effective participation in co-management decision-making a challenge.     

Cornmeal-induced resistance to ciprofloxacin and erythromycin in enterococci

Triclosan is used as an antibacterial agent in household items and personal care products. Since this compound is found in maternal milk of humans and bodies of wild animals, there is growing concern among some consumer groups and scientific community that triclosan is adverse for humans and wild animals. In order to estimate adverse actions of triclosan, the effects of triclosan on intracellular Zn²⁺ concentration and cellular thiol content were studied in rat thymocytes by the use of flow cytometer with appropriate fluorescent probes. Triclosan at 1-3 μM (sublethal concentrations) increased the intensity of FluoZin-3 fluorescence (intracellular Zn²⁺ concentration) and decreased the intensity of 5-chloromethylfluorescein (5-CMF) fluorescence (cellular thiol content). Negative correlation (r = −0.985) between triclosan-induced changes in FluoZin-3 and 5-CMF fluorescences was found. Removal of external Zn²⁺ did not significantly affect the triclosan-induced augmentation of FluoZin-3 fluorescence, suggesting an intracellular Zn²⁺ release by triclosan. These actions of triclosan were similar to those of H₂O₂ and triclosan significantly potentiated the cytotoxicity of H₂O₂. Therefore, the results may suggest that triclosan at sublethal concentrations induces oxidative stress that decreases cellular thiol content, resulting in an increase in intracellular Zn²⁺ concentration by Zn²⁺ release from intracellular store(s). Since recent studies show many physiological roles of intracellular Zn²⁺ in cellular functions, the triclosan-induced disturbance of cellular Zn²⁺ homeostasis may induce adverse actions on the cells.     

New methodological improvements in the Microtox® solid phase assay

The classic Microtox® solid phase assay (MSPA) based on the inhibition of light production of the marine bacteria recently renamed Aliivibrio fischeri suffers from various bias and interferences, mainly due to physico-chemical characteristics of the tested solid phase. To precisely assess ecotoxicity of sediments, we have developed an alternative method, named Microtox® leachate phase assay (MLPA), in order to measure the action of dissolved pollutants in the aqueous phase. Two hypotheses were formulated to explain the observed difference between MSPA and MLPA results: a real ecotoxicity of the solid phase or the fixation of bacteria to fine particles and/or organic matter. To estimate the latter, flow cytometry analyses were performed with two fluorochromes (known for their ability to stain bacterial DNA), allowing correction of MSPA measurements and generation of new (corrected) IC50. Comparison of results of MLPA with the new IC50 MSPA allows differentiating real ecotoxic and fixation effect in classic MSPA especially for samples with high amount of fines and/or organic matter.