Vitellogenesis in the deep-sea shark Centroscymnus coelolepis

At present, information on the reproductive physiology of Centroscymnus coelolepis, which is one of most important and widespread deep-sea shark species, is completely lacking. In this study, we investigated vitellogenesis, a key step in the reproduction biology of fishes. Specimens of C. coelolepis were collected at 2850 m depth in the Western Mediterranean Sea. The size of the collected sharks (range: 35.5-65.0 cm TL) was much lower than those typically reported for the Atlantic and Pacific Oceans. The marked distinctiveness of Mediterranean and Atlantic/Pacific populations was reflected by the achievement of sexual maturity at a smaller size in Mediterranean specimens. The examination of cytoplasmatic components of oocytes indicated that vitellogenin uptake in the ovary started when oocytes reached 14 mm in diameter. Only reproductive females displayed a significant relationship between plasmatic vitellogenin and gonadal development, suggesting that vitellogenesis in C. coelolepis is a discontinuous process. Oestradiol levels were tightly coupled with gonadal development, underlining the importance of this hormone in controlling vitellogenesis. All these findings suggest that vitellogenesis in this yolk-sac viviparous shark might occur with similar mechanisms of oviparous vertebrates.     

Neanderthal medics? Evidence for food, cooking, and medicinal plants entrapped in dental calculus

Neanderthals disappeared sometime between 30,000 and 24,000?years ago. Until recently, Neanderthals were understood to have been predominantly meat-eaters; however, a growing body of evidence suggests their diet also included plants. We present the results of a study, in which sequential thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) and pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) were combined with morphological analysis of plant microfossils, to identify material entrapped in dental calculus from five Neanderthal individuals from the north Spanish site of El Sidrón. Our results provide the first molecular evidence for inhalation of wood-fire smoke and bitumen or oil shale and ingestion of a range of cooked plant foods. We also offer the first evidence for the use of medicinal plants by a Neanderthal individual. The varied use of plants that we have identified suggests that the Neanderthal occupants of El Sidrón had a sophisticated knowledge of their natural surroundings which included the ability to select and use certain plants.     

Environmental Impact Assessment of Polylactide(PLA)/Chicken Feathers Biocomposite Materials

The aim of this study is to analyse the environmental impacts (EIs) of the process of preparation of new biocomposite materials obtained from polylactide (PLA) and chicken feathers (CFs). Two CFs stabilization methods and different percentages of CFs have been studied. The EIs of these new composites were compared to the impact of virgin PLA. Cradle-to-gate life cycle inventories were assessed for 0–35% v/v of CFs in a CFs/PLA biocomposite. Two CFs stabilization processes, autoclave and surfactant, were tested and compared with the aim to prioritize one of them from the environmental point of view. A composite plate of 184?×?184?×?2.2 mm³ was defined as the functional unit. Autoclave stabilization process exhibited lower environmental impact compared with surfactant stabilization process mainly due to both the lower requirements of electricity and water and the reduced pollution loads of the generated wastewater. Thus, the autoclave process was selected as the standard method when comparing the EIs of the proposed CFs/PLA biocomposites. In this sense, the addition of CFs to PLA matrix proportionally reduces all the EIs compared to pure PLA due to the replacement of PLA with CFs. This behaviour can be explained because the PLA production accounts for the 99% of the impact of the biocomposite. Consequently, CFs conveniently stabilized might be an alternative raw material to prepare CFs/PLA biocomposites with less environmental impact compared to pure PLA.     

Seasonal rhythm in a Mediterranean coastal fish community as monitored by a cabled observatory

To adequately describe seasonal rhythms in habitat utilization by fish communities, observations are needed that occur at a relatively high frequency and over large temporal windows. For the first time, images collected from a cabled video-observatory (the western Mediterranean expandable SEAfloor OBservatory; OBSEA) were utilized to examine habitat utilization by coastal fishes. Over the course of a year, hourly digital images of the water column and an artificial reef were obtained during daylight hours. For each image, the total number of species, an estimate of the abundance of individuals, and the Shannon Diversity Index were quantified. A total of 22 fish species commonly associated with nearshore western Mediterranean habitats were identified, and significant spatial (water column vs. artificial reef), seasonal (spring, summer, autumn, and winter), and daily (morning, midday, and sunset) differences in habitat utilization occurred. Four species (Diplodus vulgaris, Diplodus cervinus, Diplodus sargus, and Scorpaena porcus) were associated with the artificial reef, while one species (Pagrus pagrus) chiefly occurred in the water column. Chromis chromis and Diplodus annularis occurred at the site more frequently in the winter and autumn, respectively, while 14 other species utilized the site more frequently in either the spring (8 spp) or summer (6 spp). In addition, Dentex dentex and Spicara maena occurred more frequently at sunset, while D. cervinus displayed a crepuscular rhythm (occurring more frequently in the morning and at sunset). Species diversity was highest in the summer and lowest in the winter. If not taken into account when planning in situ sampling, such seasonal and/or diel differences may lead to spurious estimates of population sizes and biodiversity. We suggest that cabled video-observatories offer a non-invasive and reliable technology for faunistic sampling and population assessment in coastal water of the Mediterranean and likely elsewhere.     

Heterozygosity‐Fitness Correlations and Inbreeding Depression in Two Critically Endangered Mammals

The relation among inbreeding, heterozygosity, and fitness has been studied primarily among outbred populations, and little is known about these phenomena in endangered populations. Most researchers conclude that the relation between coefficient of inbreeding estimated from pedigrees and fitness traits (inbreeding‐fitness correlations) better reflects inbreeding depression than the relation between marker heterozygosity and fitness traits (heterozygosity‐fitness correlations). However, it has been suggested recently that heterozygosity‐fitness correlations should only be expected when inbreeding generates extensive identity disequilibrium (correlations in heterozygosity and homozygosity across loci throughout the genome). We tested this hypothesis in Mohor gazelle (Gazella dama mhorr) and Iberian lynx (Lynx pardinus). For Mohor gazelle, we calculated the inbreeding coefficient and measured heterozygosity at 17 microsatellite loci. For Iberian lynx, we measured heterozygosity at 36 microsatellite loci. In both species we estimated semen quality, a phenotypic trait directly related to fitness that is controlled by many loci and is affected by inbreeding depression. Both species showed evidence of extensive identity disequilibrium, and in both species heterozygosity was associated with semen quality. In the Iberian lynx the low proportion of normal sperm associated with low levels of heterozygosity was so extreme that it is likely to limit the fertility of males. In Mohor gazelle, although heterozygosity was associated with semen quality, inbreeding coefficient was not. This result suggests that when coefficient of inbreeding is calculated on the basis of a genealogy that begins after a long history of inbreeding, the coefficient of inbreeding fails to capture previous demographic information because it is a poor estimator of accumulated individual inbreeding. We conclude that among highly endangered species with extensive identity disequilibrium, examination of heterozygosity‐fitness correlations may be an effective way to detect inbreeding depression, whereas inbreeding‐fitness correlations may be poor indicators of inbreeding depression if the pedigree does not accurately reflect the history of inbreeding. Correlaciones Heterocigosidad‐ Adaptabilidad y Depresión Endogámica en Dos Especies de Mamíferos Críticamente en Peligro     

Review: recent advances in biogas purifying technologies

Biomethane production through biogas upgrading is a promising renewable energy for some industries which could be part of the equilibrium needed with fossil fuels consumption to achieve a sustainable society. This paper presents a comprehensive list of biogas upgrading technologies focused on carbon dioxide removal as well as recent advances reported by researcher with wide expertise in this topic. Additionally, an extensive costs–performance comparison among the technologies studied is discussed. Among the different alternatives, chemical scrubbing stood out to achieve high biomethane purities while cryogenic technologies proved to be effective against methane losses. Regarding the different costs, water scrubbing and membrane separation seem to be the most affordable techniques.     

Deployment of parabolic trough concentrated solar power plants in North Africa – a case study for Libya

The study addresses the potential of using concentrated solar power plants (CSPs) as a sustainable alternative of clean energy generation in the Mediterranean region and, in particular, in its North Africa shore. This location presents attractive conditions for the installation of CSPs, in particular high solar irradiation, good manpower concentration, and proximity and availability of water resources for condenser cooling. Energetic, exergetic, and economic analyses were conducted taking into consideration a particular type of CSPs - the parabolic trough concentrated solar power plant, which incorporates the most proven technology and it is already used in Southern Europe (Spain). In addition, the study considered the impact of project financing and incentives on the cost of energy. The combination of higher values for performance and potentially lower levelized cost of electricity (LCE) for the North Africa Mediterranean Rim than the South of Spain region can yield a very favorable return for the invested capital. Tripoli compared to Almeria presented superior performance and potentially lower LCE values ($0.18/kWh versus $0.22/kWh). This is significant, even when it is taken into consideration the fact that the plant in Tripoli, despite a relatively modest capacity factor of 34%, has a large gross power output of 173,886 MWhe. In addition, the implementation at the Tripoli location of a plant similar to the Anadsol plant has a slight advantage (2–3%) in terms of overall efficiency.     

Presence of Torque Teno Virus (TTV) in Tap Water in Public Schools from Southern Brazil

Torque teno virus (TTV) was surveyed in tap water collected in schools from three municipalities located in the south of Brazil. TTV genomes were found in 11.7 % (4/34) of the samples. TTV DNA was detected in 10.5 % (2/19) of the samples collected at the city of Caxias do Sul and in 25 % (2/8) of the samples from Pelotas. Those cities have a low rate of sewage treatment. All samples from Santa Cruz do Sul, which has nearly 92 % of its sewage treated, were negative. These results suggest that the amount of sewage treated may have an effect on the detection rates of TTV DNA in drinking water in a given urban area, showing a mild negative correlation (r = ?0.76), when comparing the percentage of sewage treatment to the detection of TTV genomes. The detection rate of TTV was also compared with Escherichia coli, showing a strong correlation (r = 0.97), indicating that TTV may be a suitable marker of fecal contamination.