Urinary 8-oxo-7, 8-dihydro-2′-deoxyguanosine concentrations after frying of bacon,a pilot study

Cooking fumes contain compounds that may give rise to oxidative stress and mutations when inhaled. The aim of this study was to evaluate if cooking fumes from frying of bacon induce oxidative stress by measurement of urinary 8-oxo-7,8-dihydro-2 deoxyguanosine, a marker of oxidatively damaged DNA. Three non-smoking women fried bacon for 3 h. Urine samples were taken as early morning void at the same time on four days; the morning before frying, the morning after first frying, the morning after three days of frying and one week after first urine sample. 8-Oxo-7,8-dihydro-2 deoxyguanosine, 1-hydroxypyrene and 2-hydroxyphenanthrene, metabolites of polycyclic aromatic hydrocarbons, were measured by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). 8-Oxo-7,8-dihydro-2 deoxyguanosine correlated weakly with concentrations of 1-hydroxypyrene (r = 0.31, p = 0.042), but it did not correlate with 2-hydroxyphenanthrene (r = ?0.074; p = 0.64). Average urinary 8-oxo-7,8-dihydro-2 deoxyguanosine concentrations increased from the day before frying (16.3 ± 4.2 nmol/L) to the third day of frying (26.2 ± 10.2 nmol/L), although not statistically significantly. Our pilot study shows that frying of bacon may result in increased oxidative stress which further emphasises the possible carcinogenic potential of cooking fumes.     

Plasma concentrations of sucralose in children and adults

We aimed to measure concentrations of the commonly used artificial sweetener sucralose, following ingestion of doses reflecting a range of consumption and to compare concentrations in children and adults. Eleven adults consumed 355 mL water containing 0 mg (control), 68, 170, or 250 mg sucralose (equivalent to 1–4 diet sodas). A second group of adults (n = 11) consumed 355 mL Diet Rite Cola? (68 mg sucralose and 41 mg acesulfame-potassium (ace-K)) or 68 mg sucralose and 41 mg ace-K in seltzer. Beverages were provided at separate visits in randomized order, prior to an oral glucose tolerance test. Eleven children consumed 0 or 68 mg sucralose in 240 mL water, in an identical study design. Blood was collected before beverage ingestion and serially for 120 min. Sucralose doses (corrected for weight) resulted in similar plasma concentrations in children and adults. Concentrations were comparable whether sucralose was administered in water, combined with ace-K, or in diet soda. Due to their lower body weight and blood volume, children have markedly higher plasma sucralose concentrations after the consumption of a typical diet soda, emphasizing the need to determine the clinical implications of sucralose use in children.     

The selection of management strategies for controlling nematodes in cereals

Several genera and species of plant-parasitic nematodes cause losses in grain yield in cereals; some are of relatively minor importance (e.g. Anguina tritici (Steinbuch) Chitwood, the cause of “ear cockle” in wheat), while others such as the cereal cyst nematode (CCN) (Heterodera avenae Woll.) have a wide geographic distribution, infest extensive areas, and may cause losses valued at millions of dollars. Some of these nematodes are difficult to control because the measures that might be used are uneconomic to apply or are impractical. The control of CCN, however, can be achieved, and several successful strategies have been developed in parts of Europe and in Australia. The various measures available to Australian cereal growers include: crop rotation, resistant cultivars, manipulation of sowing dates, use of nematicides, and reduced cultivation. The selection of an appropriate management strategy for the control of CCN is influenced by factors such as: climate, cereal species grown, yield potential, rotations practised and the availability of alternative crops, pathotype present, farm size, availability of resistant cultivars, nematicides registered, and the availability of suitable equipment for their application.     

Heavy metal contamination of soils and crops from auto traffic,sewage sludge,pig manure and chemical fertilizer

The present paper describes heavy metal contamination of soils and crops resulting from auto traffic, sewage sludge, pig manure and chemical fertilizers, in three separate experiments.The first experiment revealed that although the ADDT (average annual daily traffic) value of the Agricultural Area was lower, higher concentrations of Cu, Cd, Zn, Pb and Mn were obtained in soil when compared with the Commercial-Residential Area. Contaminations of trace metals from various source, e.g., chemical fertilizer, animal manure, night soil and pesticides of the nearby agricultural land were suspected.The second experiment compared the effects of adding a commercial fertilizer and digested sludge which contain substantial amounts of various trace metals to a sandy loam for growing Chinese radish. The results indicated that repeated applications of the fertilizer elevated the contents of various trace metals in the roots of Chinese radish. The phenomenon was further aggravated when digested sludge was also incorporated.The third experiment compared the contents of trace metals in the root and leaf tissues of tree seedlings (Acacia confusa) added with a single dose of the trace metalenriched fertilizer, and different portions of pig manure compost. It was revealed that the fertilizer-treated tree seedlings had a higher content of trace metals in the tissues than those amended with manure compost. Although the metal contents of soil increased as more manure compost was added, the contents in plant tissues did not increase correspondingly.     

Chlorophyll-bleaching by atmospheric pollutants and sunlight

The Science of Nature -     

Prenatal diagnosis of campomelic dysplasia by ultrasonography

Consanguineous partners had a boy with campomelic dysplasia who died of increasing respiratory distress soon after birth. The next pregnancy was monitored frequently by ultrasonography and a healthy male infant was born at term. During a further pregnancy, ultrasonography suggested campomelic dysplasia in the 16th week of gestation. This was confirmed in the 18th week. The pregnancy was terminated and the fetus showed the typical radiological, anatomical and histological findings.     

Prenatal pleural effusion associated with congenital pulmonary lymphangiectasia

A pleural effusion associated with congenital pulmonary lymphangiectasia was detected in a fetus in utero but was absent at the time of delivery. The pleural effusion was unilateral although the disease involved both lungs. In this case there was an association between polyhydramnios and congenital pulmonary lymphangiectasia.     

Microphthalmia–prenatal ultrasonic diagnosis: A case report

Prenatal real-time ultrasonographic diagnosis of microphthalmia is presented. Diagnosis was made at 18 weeks' gestation in a fetus of a patient with a previous infant affected with the syndrome of cryptophthalmia with absence of septum nasi and ambiguous genitalia (Fraser syndrome). Recognition of microphthalmia as a part of Fraser syndrome and the easy visualization of fetal facial bones and orbits in the second trimester made the diagnosis possible.     

The fetal behavioural states: An ultrasonic study

In order to accurately detect the fetal behavioural state, we simultaneously measured fetal heart rate and multiple fetal activities in 27 healthy pregnant women at 38 to 40 weeks of gestation. We ultrasonically identified gross body movements, breathing movements and micturition. Analysis of fetal heart rate allowed us to distinguish two different patterns of fetal behaviour: active and quiet phases. The frequency distribution of the analysed fetal events was significantly different in these two phases. These data suggest that a complete biophysical profile of the fetus is effective in differentiating behavioural states and may improve the predictive accuracy of fetal heart rate analysis alone.     

The cerebro-hepato-renal (Zellweger) syndrome: Prenatal detection based on impaired biosynthesis of plasmalogens

Prenatal diagnosis of the cerebro-hepato-renal (Zellweger) syndrome has been performed in 10 pregnancies at risk by measuring both the activity of acyl CoA: dihydroxyacetonephosphate acyltransferase (DHAP-AT) and the de novo plasmalogen biosynthesis, either in cultured amniotic fluid cells or in fibroblasts cultured from a chorionic villus biopsy. In 7 of the pregnancies both tests indicated no abnormality. All 7 continued to term and normal infants were delivered. However, in amniotic fluid cells from 2 fetuses affected by Zellweger syndrome unequivocal differences from control values were found. The activity of DHAP-AT was clearly deficient and the de novo plasmalogen biosynthesis was impaired. In one pregnancy at risk prenatal diagnosis was performed during the first trimester by measuring both the DHAP-AT activity and the de novo plasmalogen biosynthesis in fibroblasts cultured from a chorionic villi biopsy. From the deficient DHAP-AT activity and the impaired de novo plasmalogen biosynthesis it was concluded that the fetus was affected. This was confirmed biochemically after induced abortion. It can be concluded that measurement of the DHAP-AT activity and the de novo plasmalogen biosynthesis provides convenient methods for the early prenatal detection of Zellweger syndrome.