Environmental Monotoring and Assessment Program: Current Status of Virginian Province (U.S.) Estuaries

Monitoring of indicators of the ecological condition of bays, tidal rivers, and estuaries within the Virginian Biogeographic Province (Cape Cod, Massachusetts to Cape Henry, Virginia) was conducted annually by the U.S. EPA's Environmental Monitoring and Assessment Program (EMAP) during the summer months of 1990 to 1993. Data were collected at 425 probability-based stations within the Province. Indicators monitored included water quality (temperature, salinity, water clarity, and dissolved oxygen concentration), sediment contamination, sediment toxicity, benthic community structure, fish community structure, and fish gross external pathology. Data were used to estimate the current status of the ecological condition of Virginian Province estuarine resources, and provide a baseline for identifying possible future changes. Estimates, with 95% confidence limits, of the areal extent of impacted resources within the Province are provided. Data are also presented by estuarine class (large estuaries, small estuarine systems, and large tidal rivers). Results show that, overall, approximately half of the estuarine waters of the Virginian Province can be classified as impacted based on multiple indicators, with hypoxia being the major stressor.     

Toxicity study of the oil dispersant Corexit 9527 on Macrobrachium rosenbergii (de Man) egg hatchability by using a flow-through bioassay technique

The effect of the oil-spill dispersant Corexit 9527 on egg-hatching rate of Macrobrachium rosenbergii (de Man) was studied by using an innovated flow-through bioassay technique. This bioassay method relies on the fact that M. rosenbergii fertilized eggs when detached from the mother prawn were able to hatch artificially. The flow-through system generated a stable and good water quality environment for hatching the eggs successfully. The Corexit 9527 had a pronounced effect on hatching rate of the M. rosenbergii eggs. In the control, the hatching rate of the eggs was 95.55% +/- 1.74%. However, it was reduced drastically with increasing concentrations of Corexit 9527. A 100% inhibition of egg hatchability was found when the level of Corexit 9527 was higher than 250 mg litre(-1). The EC(50) and the EC(95) values estimated by the probit method were 80.4 +/- 5.5 mg litre(-1) and 193.5 +/- 39.9 mg litre(-1) respectively (P = 0.05). The recommended safety level of Corexit 9527 for M. rosenbergii in Malaysian estuarine waters is below 40 mg litre(-1).     

Comparison of E. coli, enterococci, and fecal coliform as indicators for brackish water quality assessment.

Escherichia coli (E. coli), enterococci, and fecal coliform data were collected and compared as potential indicators for swimmablility assessment of a brackish waterbody (Lake Pontchartrain, Louisiana). These indicators were measured during lake background conditions, in stormwater runoff (before dilution with lake water), and in the outfall plume within the lake following storm events. Microbial indicator titers associated with suspended particles and lake-bottom sediments were also investigated. Overall reduction rate constants for fecal coliform, E. coli, and enterococci in lake water and sediment were measured and reported. Attachment of microbial indicators to suspended matter and subsequent sedimentation appeared to be a significant fate mechanism. A slower reduction rate of indicator organisms in sediment further suggested that bottom sediment may act as a reservoir for prolonging indicator organism survival and added concern of recontamination of overlaying waters due to potential solids resuspension. Results indicated that enterococci might be a more stable indicator than E. coli and fecal coliform and, consequently, a more conservative indicator under brackish water conditions.     

Long-term field characterization of tapered element oscillating microbalance and modified tapered element oscillating microbalance samplers in urban and rural New York State locations

Long-term field comparisons of continuous and integrated filter measurements of mass concentrations of particulate matter (PM) with an aerodynamic diameter less than or equal to 2.5 microm (PM2.5) were performed at rural and urban sites in New York State. Two versions of the continuous tapered element oscillating microbalance (TEOM) mass monitor are deployed at each site, in addition to Federal Reference Method filter samplers. Data are grouped into monthly averages to retain and demonstrate seasonal differences. Strong seasonal dependence is observed-the TEOM monitors with the heated sensors are biased systematically low with respect to the Federal Reference Method measurements during the cold season. For the rural site, the average bias for the sample equilibration system (SES)-equipped and standard TEOM monitors is 14 and 24%, respectively. At this location, the TEOM monitor measurements were biased low for all 34 months. For the urban site, the average bias for the SES and standard TEOM monitors is 8 and 18%, respectively. At this location, the TEOM monitor measurements are as likely to be biased high as low during the warm-season months. The hour averaged data from the two versions of the TEOM monitor are also compared, and also indicate that the SES-equipped version of the TEOM monitor captures 7-11% more PM2.5 mass at these locations.     

Polybrominated diphenyl ethers in two species of marine top predators from England and Wales

During an earlier study, polybrominated diphenyl ethers were detected at high concentrations in fish and sediments downstream of a manufacturing site in NE England. We have now undertaken analysis of 14 tri- to heptabromodiphenyl ether congeners in tissues of two species of marine top predators exposed to these compounds through their consumption of fish. In this paper we report data for 47 cormorants and 60 harbour porpoises from England and Wales, sampled during the period 1996-2000. Concentrations of the summed congeners ranged from 1.8 to 140 microg kg(-1) wet weight in cormorant livers, and from not detected to 6900 microg kg(-1) wet weight in porpoise blubber. The major congeners present were generally BDE47, BDE99 and BDE100. There was little correlation between concentrations of chlorobiphenyls (as the sum of 25 individual congeners) and the sum of the 14 BDE congeners determined, particularly for the porpoises.     

Polychlorinated biphenyl (PCB) congeners and chlorinated pesticides in a cod liver oil reference material

A cod liver oil reference material, Standard Reference Material (SRM) 1588, which was issued by the National Institute of Standards and Technology (NIST) in 1989, has certified concentrations for five polychlorinated biphenyl (PCB) congeners and ten chlorinated pesticides. Recently, this SRM has been extensively characterized by additional analyses at NIST and by analyses at the Canadian Wildlife Service (CWS) where the SRM was used as a control sample during routine analyses of wildlife tissue specimens. In this paper the results from NIST and CWS are compared and the concentrations for over 40 PCB congeners and 20 chlorinated hydrocarbons are reported.     

Accumulation of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid-co-4-hydroxyvaleric acid) by mutants and recombinant strains ofAlcaligenes eutrophus

Alcaligenes eutrophus accumulated a terpolyester of 3-hydroxybutyric acid (3HB), 3-hydroxyvaleric acid (3HV), and 4-hydroxyvaleric acid (4HV) during cultivation with 4HV as carbon and energy source under nitrogen starvation. The polyester accumulated by wild-type strains under these conditions contained 4HV at a molar fraction of approximately 5 mol% only. A catabolic pathway of 4HV was postulated, which included the activation of 4HV to 4HV-CoA and a conversion of 4HV-CoA to 3HV-CoA. Tn5::mob-induced mutants were isolated fromA. eutrophus HF39, which were affected in 4HV and/or valeric acid catabolism. Among 83 mutants were 27 4HV-negative or 4HV-leaky mutants; two mutants were identified which accumulated a terpolyester with a molar fraction of 10.1 to 22.7 mol% 4HV. In addition, a further increase in the molar fraction of 4HV in poly(3HB-co-3HV-co-4HV) and a two- to fourfold increase in the PHA synthase activity were monitored in these mutants or others and also in HF39, if the cells were complemented with the hybrid plasmid pHP1014::PP1, which contained the PHA biosynthesis genes ofA. eutrophus H16. Application of mutagenesis plus recombinant DNA techniques resulted in the accumulation of a terpolyester with up to 30 mol% 4HV and with approximately equimolar fractions of 3HB, 3HV, and 4HV.     

Photodynamic action of some sensitizers by photooxidation of luminol

We report the development of a novel simple experimental method which allows the comparison of new photosensitizers based on their production of reactive oxygen species. A high-performance liquid chromatography (HPLC) assay permits the monitoring of several substances (sensitizer, reactant and oxidized end product) simultaneously on a single chromatogram. Photoreactions were monitored throughout their course by the HPLC assay surveying the sensitizers' efficiency of singlet oxygen production by the oxidative decomposition of luminol. Several photosensitizers were tested: Rose Bengal, Methylene Blue, Protoporphyrin IX, Photosan III, Photofrin, Hypericin and Pseudohypericin. Additionally, photoreactions were monitored by a standard pO₂ detection system. The measurements of the two detection methods were strongly correlated. Rose Bengal proved to be the most efficient photosensitizer, clearly decreasing the luminol concentration and causing a corresponding increase in aminophthalic acid. Our experiments show that when factors necessary for photochemical reactions are absent or are blocked (antioxidants), no reaction can be detected.     

Effect of organic acid on Cd toxicityin tomato and bean growth

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Ultrastructural study and calcium and cadmium localization in the marine coccolithophoridCricosphaera elongata

Cells of the coccolithophoridCricosphaera elongata (Droop) Braarud (Prymnesiophyceae) were concentrated by means of tangential ultrafiltration. Ultrastructural features of control cells were typical of the genusCricosphaera. The flagellar apparatus consists of two flagella, a bulbous haptonema, an axosome and a basal helical band, as recently reported in the literature for another coccolithophorid. We observed a very-well developed Golgi apparatus, displaying all the features of the peculiar Golgi of the Prymnesiophytes. Observations with the light microscope did not reveal any difference between control algae and algae contaminated with 1, 10 and 20 µmol Cd 1^–1 (i.e., 112 µg 1^–1, 1.12 and 2.24 mg Cd 1^–1) for 48 h. However, algae treated with the highest cadmium concentration exhibited loss of motility after 6 h contact, but without apparent ultrastructural damage. Qualitative X-ray microanalysis of the dense granules present in Golgi vacuoles of control cells demonstrated the presence of calcium and chlorine and, at a lower level, sulphur and aluminium. The same elements were also identified in the dense part of the lysosomes. In situ,C. elongata produces coccoliths; this did not occur in culture. However, the coccolith-generating apparatus (Golgi complex and Ca²⁺) was present in culture, and the cells were able to synthesize unmineralized scales.