Long-term tillage effects on atrazine and fluometuron sorption in Coastal Plain soils

Conservation tillage (CnT) management practices are known to increase levels of soil organic matter (SOM) in southeastern Coastal Plain soils. Plant residues in CnT systems accumulate at the surface and, with time, will form a layer enriched in SOM. The authors hypothesize that herbicide sorption will be highest in this SOM-enriched zone of CnT systems when compared to sorption at a similar depth in conventional tillage (CT) systems. The objective was to characterize the impact of two different tillage systems, CnT and CT, on sorption of atrazine [6-chloro-N-ethyl-N′-(1-methylethyl)-1,3,5-triazine-2,4-diamine] and fluometuron [N,N-dimethyl-N′-3-(trifluoromethyl)phenyl urea] in plots of Norfolk loamy sand (fine-loamy, siliceous thermic Typic Kandiudult). The plots have been under CnT and CT management for 18 yrs. Bulk (0–15 cm) and five equal incremental soil samples to a 15 cm depth were collected from 10 CnT and 10 CT plots, and the atrazine (ATR) and fluometuron (FLMT) sorption coefficients (K_d) were measured using batch equilibration. Significantly higher herbicide K_d values occurred in the CnT 0–3 cm samples, indicating that the highest amount of herbicide sorption occurred in the top few cm of soil. This corresponded to the stratified soil organic carbon (SOC) contents in topsoil of the CnT plots. In addition, analyses of covariance using SOC as the covariant to test for tillage effects indicated complex interactions among SOC, tillage, and depth. Those results confirm that tillage and soil depth will affect SOC contents of a Norfolk loamy sand, which correspondly will influence the magnitude of ATR and FLMT sorption.     

Atmospheric concentrations of PCDDs/PCDFs in southern California.

A comprehensive air toxics measurements program designed to establish baseline concentrations of atmospheric polychlorinated dioxins and dibenzofurans (PCDDs/PCDFs) in the South Coast Air Basin has been completed. The program utilized state-of-the-art air sampling and laboratory analysis techniques (HRGC/HRMS) to quantify the fifteen 2,3,7,8-substituted PCDDs/PCDFs congeners of primary toxicological significance. This study, which included nine discrete sampling sessions between December 1987 and March 1989, provides the first systematic assessment of ambient PCDDs/PCDFs concentrations in the state of California. The highest PCDDs/PCDFs concentrations noted during this study occurred in December 1987. This period was dominated by off-shore air flows, suggesting a regional air mass and transport phenomena. Concentrations of the PCDDs/PCDFs were diminished markedly in subsequent sampling sessions where air flow patterns were primarily of on-shore or of coastal origin. Ambient PCDDs/PCDFs concentrations, expressed as toxic equivalents, were highest during the December 1987 sampling period. The El Toro monitoring site, located approximately 40 miles southeast of Los Angeles, consistently showed the lowest measured ambient PCDDs/PCDFs concentrations and toxic equivalents values. In the majority of the sessions and samples examined the PCDDs/PCDFs congener profiles strongly suggest combustion source influences. Typical of combustion source profiles, 1,2,3,4,6,7,8-HpCDD was the predominant 2,3,7,8-substituted species and most prevalent PCDD after OCDD. The congener of highest toxicological significance, 2,3,7,8-TCDD, was reported below the 10-20 fg/m3 detection limit for most of the ambient air samples selected for analysis.     

Evaluating peats for their capacities to remove odorous compounds from liquid swine manure using headspace "solid-phase microextraction".

This paper reports on research designed to investigate the capacities of different highly characterized peats to remove odorous compounds from liquid swine manure (LSM). Peat types representing a wide range of properties were tested in order to establish which chemical and physical properties might be most indicative of their capacities to remediate odors produced by LSM. Eight percent slurries (of peat/LSM) were measured for odor changes after 24 hours using odor panel and GC/MS-Solid-phase microextraction (GC/MS-SPME) analysis. The GC/MS-SPME and odor panel results indicated that, although all peats tested in this study were found to be effective at removing odor-causing compounds found in LSM, some peats tended to work better than others. Overall, the peats that were the most effective at removing odor-causing compounds tended to have lower bulk densities, ash contents, fulvic acids contents, and guaiacyl lignins contents, and higher water holding capacities, hydraulic conductivities, "total other lignins" contents, hydrogen contents, carbon contents, and total cellulose contents. GC/MS-SPME analysis was found to be a reasonably inexpensive and efficient way of conducting this type of research. It allows one to identify a large number of the odor-causing compounds found in LSM, and more importantly, to detect with some precision specific differences in the amounts of these compounds between peat types.     

Prenatal diagnosis of tetrasomy 12p by in situ hybridization: Varying levels of mosaicism in different fetal tissues

Prenatal diagnosis of tetrasomy 12p is complicated by the discrimination of the 12p isochromosome from the duplication 21q as well as the level of mosaicism demonstrated in the particular tissue sampled. In this disease, a high percentage of chromosomally abnormal cells are generally found in fibroblastic cells, but lymphocyte karyotypes from the same individual may be normal. We report on the pregnancy of a 37-year-old female who presented to our centre at 16 weeks' gestation for genetic amniocentesis. Sonography of the fetus revealed dextrocardia and diaphragmatic hernia. Chromosome analysis of amniocytes demonstrated mosaicism of a 47,XY,+i(12p) line in 80 per cent of cells and a normal male line (20 per cent), consistent with the Pallister-Killian syndrome. Following termination, a 220 g male fetus of 18 weeks was examined. A flattened nose and low-set ears were noted. In situ hybridization with a chromosome 12 centromeric probe in lymphocytes and skin cells unequivocally confirmed the karyotype and showed the presence of a single centromere in the abnormal chromosome, suggesting a true isochromosome. Chromosome analysis of various fetal tissues was performed and the following percentages of abnormal cells were found: skin 100 per cent, chorion 50 per cent, placenta 30 per cent, and blood 80 per cent. The high frequency of tetrasomic cells in fetal blood at this early gestational age is noteworthy, since most reports of this syndrome show a very low percentage of abnormal cells postnatally.     

Missed connections: recombination and human aneuploidy

The physical exchange of DNA between homologs, crossing-over, is essential to orchestrate the unique, reductional first meiotic division (MI). In females, the events of meiotic recombination that serve to tether homologs and facilitate their disjunction at MI occur during fetal development, preceding the MI division by several decades in our species. Data from studies in humans and mice demonstrate that placement of recombination sites during fetal development influences the likelihood of an MI nondisjunction event that results in the production of an aneuploid egg. Here we briefly summarize what we know about the relationship between aneuploidy and meiotic recombination and important considerations for the future of human assisted reproduction.     

Nitrous oxide accumulation in soils from riparian buffers of a coastal plain watershed carbon/nitrogen ratio control

Riparian buffers are used throughout the world for the protection of water bodies from nonpoint-source nitrogen pollution. Few studies of riparian or treatment wetland denitrification consider the production of nitrous oxide (N2O). The objectives of this research were to ascertain the level of potential N2O production in riparian buffers and identify controlling factors for N2O accumulations within riparian soils of an agricultural watershed in the southeastern Coastal Plain of the USA. Soil samples were obtained from ten sites (site types) with different agronomic management and landscape position. Denitrification enzyme activity (DEA) was measured by the acetylene inhibition method. Nitrous oxide accumulations were measured after incubation with and without acetylene (baseline N2O production). The mean DEA (with acetylene) was 59 microg N2O-N kg(-1) soil h(-1) for all soil samples from the watershed. If no acetylene was added to block conversion of N2O to N2, only 15 microg N2O-N kg(-1) soil h(-1) were accumulated. Half of the samples accumulated no N2O. The highest level of denitrification was found in the soil surface layers and in buffers impacted by either livestock waste or nitrogen from legume production. Nitrous oxide accumulations (with acetylene inhibition) were correlated to soil nitrogen (r2=0.59). Without acetylene inhibition, correlations with soil and site characteristics were lower. Nitrous oxide accumulations were found to be essentially zero, if the soil C/N ratios>25. Soil C/N ratios may be an easily measured and widely applicable parameter for identification of potential hot spots of N2O productions from riparian buffers.     

Scale and sustainability of marine bioprospecting for pharmaceuticals

Collecting marine organisms for the discovery and development of pharmaceuticals has been perceived variously as sustaining and threatening conservation. Our initial expectations that marine bioprospecting might pose conservation challenges were largely not confirmed. Thousands of marine species have been collected for initial assessment, but usually only in very small amounts. Very few compounds are sufficiently promising to provoke re-collections, where volumes can be much larger. This is where conservation concerns may arise, particularly if the organism is rare, has a restricted distribution, or is targeted in one narrow area. However, industry generally seeks to avoid dependency on small populations, for economic as well as ecological reasons. Alternative supply strategies to wild capture include synthesis and culture. Mandatory collection protocols and environmental impact (stock) assessments are useful routes for management to achieve sustainable use where extraction is desirable. In general, the scanty information available suggests that marine bioprospecting for pharmaceuticals may have minimal impacts on the environment, particularly compared with those created by other pressures.     

Evaluating peats for their capacities to remove odorous compounds from liquid swine manure using headspace “solid‐phase microextraction”

Abstract

This paper reports on research designed to investigate the capacities of different highly characterized peats to remove odorous compounds from liquid swine manure (LSM). Peat types representing a wide range of properties were tested in order to establish which chemical and physical properties might be most indicative of their capacities to remediate odors produced by LSM. Eight percent slurries (of peat/LSM) were measured for odor changes after 24 hours using odor panel and GC/MS‐Solid‐phase microextraction (GC/MS‐SPME) analysis.

The GC/MS‐SPME and odor panel results indicated that, although all peats tested in this study were found to be effective at removing odor‐causing compounds found in LSM, some peats tended to work better than others. Overall, the peats that were the most effective at removing odor‐causing compounds tended to have lower bulk densities, ash contents, fulvic acids contents, and guaiacyl lignins contents,and higher water holding capacities, hydraulic conductivities, “total other lignins”; contents, hydrogen contents, carbon contents, and total cellulose contents.

GC/MS‐SPME analysis was found to be a reasonably inexpensive and efficient way of conducting this type of research. It allows one to identify a large number of the odor‐causing compounds found in LSM, and more importantly, to detect with some precision specific differences in the amounts of these compounds between peat types.     

TOXIC EFFECTS OF PETROLEUM AND SHALE JP-4 AND JP-8 AVIATION FUELS ON FATHEAD MINNOWS1

ABSTRACT: Static 96-hour median lethal concentrations were determined for the water-soluble fraction (WSF) of both petroleum-derived and shale-derived avaiation fuels (JP-4 and JP-8) using fathead minnows. JP-8 was more toxic than JP-4 except for one shale JP-4 sample that was as toxic as the JP-8. Petroleum and shale JP-8 were similar in toxicity. The toxicity of shale JP-4 was less clear. Shale JP-4 from three vendor sources revealed differing toxicity values. One shale JP-4 sample was more toxic and one less toxic than its petroleum analogue, with the third being equally toxic. Toxicity of the fuels may be enhanced by compounds in the WSF that correspond to chemicals containing 10 or more carbon atoms.