Evidence for a significant contribution of wood burning aerosols to PM2.5 during the winter season in Paris,France

Although particulate emissions from residential wood burning have become a subject of great scientific concern for a few years, data related to their impact on the air quality of large European urban centres are still missing. In the present study, we investigated the chemical and optical properties of fine (PM_2.5) carbonaceous aerosols in Paris during the 2005 winter season in order to track the presence of wood burning emissions in such a large city. The use of a seven wavelength Aethalometer allowed us to document shortwave light absorption by brown-carbon-containing organic aerosols of biomass burning origin. In particular, a well-marked diurnal pattern of the spectral dependence of light absorption, with maxima during the night, could be observed every day of the campaign and attributed to wood burning emissions. Relatively high absorption Ångstrom exponents and WSOC/OC ratios (respectively 1.25 and 0.35 on average for the period of study) also indicated the importance of biomass burning aerosols in the Paris atmosphere in winter. Finally, a rough estimate of the contribution of wood burning carbonaceous aerosols to PM_2.5 could be achieved. This contribution was found to be as high as 20 ± 10% on average at the Paris background site investigated here.     

Assessment of the contribution of land use pattern and intensity to landscape quality: use of a landscape indicator

The objective of this research work is the evaluation of the impact of landuse pattern and intensity on landscape by means of an indicator. The method used to calculate a ‘landscape indicator’ (I_land) allows to take into account the objective as well as the subjective approach of landscape. I_land corresponds to the degree of agreement between landscape supply by farmers and landscape demand by the social groups. The supply and the demand are evaluated through four criteria: ‘diversity’, ‘upkeep’, ‘openness’ and ‘heritage’. The landscape supply is calculated from data of landscape objects (punctual, linear and spatial) for each criterion recorded at the field level. The values of the four criteria for the landscape demand are allocated by the user(s) of the indicator (decision makers, regional council, social groups…) into five classes (0–4). The value of the landscape indicator is the least favourable difference between supply and demand for the four criteria. An example of calculation of the ‘landscape indicator’ for an arable farm is given. The collection of data needs 2 h with the farmer and 2 h for a survey of the farm land.     

Geotechnical properties of fresh municipal solid waste at Orchard Hills Landfill, USA

This paper presents the results of a laboratory investigation to determine the geotechnical properties of fresh municipal solid waste (MSW) collected from the working phase of Orchard Hills Landfill located in Davis Junction (Illinois, USA). Laboratory testing was conducted on shredded MSW to determine the compaction, hydraulic conductivity, compressibility, and shear strength properties at in-situ gravimetric moisture content of 44%. In addition, the effect of increased moisture content during leachate recirculation on compressibility and shear strength of MSW was also investigated by testing samples with variable gravimetric moisture contents ranging from 44% to 100%. Based on Standard Proctor tests, a maximum dry density of 420 kg/m(3) was observed at 70% optimum moisture content. The hydraulic conductivity varied in a wide range of 10(-8)-10(-4)m/s and decreased with increase in dry density. Compression ratio values varied in a close range of 0.24-0.33 with no specific trend with the increase in moisture content. Based on direct shear tests, drained cohesion varied from 31 to 64 kPa and the drained friction angle ranged from 26 to 30 degrees. Neither cohesion nor friction angle demonstrated any correlation with the moisture content, within the range of moisture contents tested. The consolidated undrained triaxial shear tests on saturated MSW showed the total strength parameters (c and phi) to be 32 kPa and 12 degrees, and the effective strength parameters (c' and phi') to be 38 kPa and 16 degrees. The angle of friction (phi) decreased and cohesion (c) value increased with the increase in strain. The effective cohesion (c') increased with increase in strain; however, the effective angle of friction (phi') decreased first and then increased with the increase in strain. Such strain-dependent shear strength properties should be properly accounted in the stability analysis of bioreactor landfills.     

Single-phase and two-phase anaerobic digestion of fruit and vegetable waste: Comparison of start-up,reactor stability and process performance

Single-phase and two-phase digestion of fruit and vegetable waste were studied to compare reactor start-up, reactor stability and performance (methane yield, volatile solids reduction and energy yield). The single-phase reactor (SPR) was a conventional reactor operated at a low loading rate (maximum of 3.5 kg VS/m³ d), while the two-phase system consisted of an acidification reactor (TPAR) and a methanogenic reactor (TPMR). The TPAR was inoculated with methanogenic sludge similar to the SPR, but was operated with step-wise increase in the loading rate and with total recirculation of reactor solids to convert it into acidification sludge. Before each feeding, part of the sludge from TPAR was centrifuged, the centrifuge liquid (solubilized products) was fed to the TPMR and centrifuged solids were recycled back to the reactor. Single-phase digestion produced a methane yield of 0.45 m³ CH₄/kg VS fed and VS removal of 83%. The TPAR shifted to acidification mode at an OLR of 10.0 kg VS/m³ d and then achieved stable performance at 7.0 kg VS/m³ d and pH 5.5–6.2, with very high substrate solubilization rate and a methane yield of 0.30 m³ CH₄/kg COD fed. The two-phase process was capable of high VS reduction, but material and energy balance showed that the single-phase process was superior in terms of volumetric methane production and energy yield by 33%. The lower energy yield of the two-phase system was due to the loss of energy during hydrolysis in the TPAR and the deficit in methane production in the TPMR attributed to COD loss due to biomass synthesis and adsorption of hard COD onto the flocs. These results including the complicated operational procedure of the two-phase process and the economic factors suggested that the single-phase process could be the preferred system for FVW.     

Contribution of a New PCR assay to the prenatal diagnosis of congenital toxoplasmosis

A polymerase chain reaction (PCR) assay has been developed for the detection of Toxoplasma gondii. The target sequence (88 bp) is part of a rDNA repetitive gene. A signal can be observed with only one parasite. It is directly and rapidly detected by electrophoresis and ethidium bromide staining. We report a prospective study of 80 documented cases of toxoplasmic seroconversions during pregnancy. The PCR assay of the amniotic fluids was compared with the current standard methods for diagnosis of fetal infection. Seventy specimens gave no PCR signal, and were negative according to prenatal tests and postnatal examinations. The presence of T. gondii was detected in ten specimens by PCR analysis. Four were confirmed by isolation of the parasite from the amniotic fluid; four by biological study of the fetal blood. For the remaining two, infection was diagnosed after birth. Together with ultrasonographic and biological data, this technique permits prenatal diagnosis within 1 day.     

Cystic hygroma simulating an encephalocele

An ultrasound examination at 17 weeks gestation on a woman with a family history of spina bifida suggested that the fetus had a closed encephalocele. Amniotic fluid alphafetoprotein, rapidly adhering cells and acetylcholinesterase gel electrophoresis were normal. The pregnancy was terminated and the fetus was found to have a large cystic hygroma. It is suggested that in counselling parents of an infant or fetus with a cystic hygroma and with a normal chromosome constitution, ultrasound examination in future pregnancies is advisable, because of the possibility of autosomal recessive inheritance.     

Perspektiven der Interferon-Forschung

Progress in understanding of interferon-mediated mechanisms over the past 20 years has been steady although slow. A major obstacle has always been the difficulty of obtaining sufficient quantities of highly purified interferon. Rational therapeutic applications therefore depend on a solution of problems connected with mass production and purification of interferon.     

Raised maternal serum alpha-fetoprotein in the absence of fetal abnormality-placental findings. A quantitative morphometric study

Ten placentae from pregnancies proceeding to term from mothers who on routine screening at 16–18 weeks gestation were found to have a raised serum AFP but no increase in amniotic fluid AFP and no fetal abnormality, were studied using morphometric techniques. The results were compared with 20 placentae from normal term pregnancies where the maternal serum AFP level was not elevated. The mean total placental volume, volume of parenchyma and villous surface area were increased in the placentae associated with a raised maternal serum AFP. More of these placentae were infarcted and the fetal-placental weight ratio was significantly lower. The hypothesis that elevation of maternal serum AFP level is related to the increase in placental size is addressed.     

Correct prenatal diagnosis of a hurler fetus where amniotic fluid cell cultures were of maternal origin

Contamination of amniotic fluid cell cultures by maternal cells can be expected to lead to misdiagnosis of fetal genotype in 0·1 to 0·5/100 cultures, when assays are carried out directly on cultured cells. Chemical analysis of the cell-free amniotic fluid supernatant may overcome this source of error and has the added advantages of speed and independence from amniotic cell culture failure. We describe a pregnancy at risk for Hurler's disease where amniotic cells cultured at amniocentesis had a female karyotype and an α-iduronidase activity towards both phenyl and 4-methylumbelliferyl substrates at the lower end of the normal range, suggesting a heterozygous fetus. An affected fetus was predicted, however, because of a high concentration of dermatan sulphate in the amniotic fluid. The discrepancy between these findings was shown to be due to maternal cell contamination of amniotic fluid cell cultures by the birth of a male infant with Hurler's disease.