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341.
Invasive plant species alter soils in ways that may affect the success of subsequent generations, creating plant-soil feedbacks. Ailanthus altissima is an invasive tree introduced two centuries ago to North America. We hypothesized that geographically distinct populations of A. altissima have established feedbacks specific to their local environment, due to soil communities cultivated by A. altissima. We collected seeds and soils from three populations in the eastern United States, and in the greenhouse reciprocally planted all families in all collected soils as well as in a control mixed soil, and in soils that had been irradiated for sterilization. There were positive plant-soil feedbacks for two populations in the live field-collected soils, but strong negative feedbacks for the third population. There were no population-level performance differences or feedbacks in the sterilized population locale soils, supporting a soil biotic basis for feedbacks and for the expression of genetic differentiation in A. altissima. If populations of Ailanthus altissima vary in the extent to which they benefit from and promote these plant-soil biota feedbacks, the interaction between invader and invaded community may be more important in determining the course of invasion than are the characteristics of either alone. 相似文献
342.
Jennifer S. Lewis Douglas Wartzok Michael R. Heithaus 《Behavioral ecology and sociobiology》2011,65(5):1061-1069
Leadership by specific individuals is thought to enhance the fitness of followers by allowing them to take advantage of the
knowledge or skills of key individuals. In general, consistent leadership is expected to occur primarily in stable groups
of related individuals where the benefits enhance the inclusive fitness of a leader. Societies with less stability in group
composition (i.e., fission–fusion groups) are less likely to feature unshared decision making. However, in situations where
frequent interactions among individuals occur (e.g., small population size and small range of movement) and/or the complexity
of the environment requires substantial experience and knowledge, consistent leadership might be expected. We tested if a
highly dynamic fission–fusion population of bottlenose dolphins (Tursiops truncatus), inhabiting a complex environment, exhibited leadership when traveling. A small number of specific individuals led group
travel more often than expected by chance, and were more likely to initiate successful direction changes of groups than following
individuals. The number of leaders in a group remained relatively constant across a wide range of group sizes and was not
affected by the number of potential leaders (i.e., those that had led previously) present in the group. Together, these results
suggest that leadership can occur in species with high rates of group fission and fusion. Therefore, the loss of key individuals
could have disproportionate effects on population dynamics. 相似文献
343.
McKenzie JH Alwis KU Sordillo JE Kalluri KS Milton DK 《Journal of environmental monitoring : JEM》2011,13(6):1739-1745
Measurement of environmental endotoxin exposures is complicated by variability encountered using current biological assay methods arising in part from lot-to-lot variability of the Limulus-amebocyte lysate (LAL) reagents. Therefore, we investigated the lot-to-lot repeatability of commercially available recombinant Factor C (rFC) kits as an alternative to LAL. Specifically, we compared endotoxin estimates obtained from rFC assay of twenty indoor dust samples, using four different extraction and assay media, to endotoxin estimates previously obtained by Limulus amebocyte lysate (LAL) assay and amounts of 3-hydroxy fatty acids (3-OHFA) in lipopolysaccharide (LPS) using gas-chromatography mass spectroscopy (GC-MS). We found that lot-to-lot variability of the rFC assay kits does not significantly alter endotoxin estimates in house dust samples when performed using three of the four assay media tested and that choice of assay media significantly altered endotoxin estimates obtained by rFC assay of house dust samples. Our findings demonstrate lot-to-lot reproducibility of rFC assay of environmental samples and suggest that use of rFC assay performed with Tris buffer or water as the extraction and assay medium for measurement of endotoxin in dust samples may be a suitable choice for developing a standardized methodology. 相似文献
344.
Gaines LG Fent KW Flack SL Thomasen JM Whittaker SG Nylander-French LA 《Journal of environmental monitoring : JEM》2011,13(1):119-127
Although urinary 1,6-hexamethylene diamine (HDA) is a useful biomarker of exposure to 1,6-hexamethylene diisocyanate (HDI), a large degree of unexplained intra- and inter-individual variability exists between estimated HDI exposure and urine HDA levels. We investigated the effect of individual and workplace factors on urine HDA levels using quantitative dermal and inhalation exposure data derived from a survey of automotive spray painters exposed to HDI. Painters' dermal and breathing-zone HDI-exposures were monitored over an entire workday for up to three separate workdays, spaced approximately one month apart. One urine sample was collected before the start of work with HDI-containing paints, and multiple samples were collected throughout the workday. Using mixed effects multiple linear regression modeling, coverall use resulted in significantly lower HDA levels (p = 0.12), and weekday contributed to significant variability in HDA levels (p = 0.056). We also investigated differences in urine HDA levels stratified by dichotomous and classification covariates using analysis of variance. Use of coveralls (p = 0.05), respirator type worn (p = 0.06), smoker status (p = 0.12), paint-booth type (p = 0.02), and more than one painter at the shop (p = 0.10) were all found to significantly affect urine HDA levels adjusted for creatinine concentration. Coverall use remained significant (p = 0.10), even after adjusting for respirator type. These results indicate that the variation in urine HDA level is mainly due to workplace factors and that appropriate dermal and inhalation protection is required to prevent HDI exposure. 相似文献
345.
Zhi-Wu Wang Seung-Hwan Lee James G. Elkins Yongchao Li Scott Hamilton-Brehm Jennifer L. Morrell-Falvey 《环境科学学报(英文版)》2013,25(5):849-856
Live cell imaging methods provide important insights into the dynamics of cellular processes that cannot be derived easily from population-averaged datasets.In the bioenergy field,much research is focused on fermentation of cellulosic biomass by thermophilic microbes to produce biofuels;however,little effort is dedicated to the development of imaging tools to monitor this dynamic biological process.This is,in part,due to the experimental challenges of imaging cells under both anaerobic and thermophilic conditions.Here an imaging system is described that integrates confocal microscopy,a flow cell device,and a lipophilic dye to visualize cells.Solutions to technical obstacles regarding suitable fluorescent markers,photodamage during imaging,and maintenance of environmental conditions during imaging are presented.This system was utilized to observe cellulose colonization by Clostridium thermocellum under anaerobic conditions at 60℃.This method enables live cell imaging of bacterial growth under anaerobic and thermophilic conditions and should be widely applicable to visualizing different cell types or processes in real time. 相似文献
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