Epithelial immunity in a marine invertebrate: a cytolytic activity from a cuticular secretion of the American horseshoe crab, <Emphasis Type="Italic">Limulus polyphemus</Emphasis>

The cuticle of the American horseshoe crab, Limulus polyphemus, is largely free of the macroscopic epibionts that rapidly colonize most solid surfaces exposed to sea water. Here, we describe a viscous surface secretion that coats the carapace of the horseshoe crab. We report methods for stimulating production of the substance, identify hemolytic and liposome-permeabilizing activities and provide a partial biochemical characterization. We propose that this secretion functions as an anti-fouling agent protecting the chitinized epithelium of the cuticle from colonization by deleterious epibionts.     

INFLUENCES OF WATERSHED,RIPARIAN‐CORRIDOR,AND REACH‐SCALE CHARACTERISTICS ON AQUATIC BIOTA IN AGRICULTURAL WATERSHEDS1

ABSTRACT: Multivariate analyses and correlations revealed strong relations between watershed and riparian‐corridor land cover, and reach‐scale habitat versus fish and macroinvertebrate assemblages in 38 warmwater streams in eastern Wisconsin. Watersheds were dominated by agricultural use, and ranged in size from 9 to 71 km² Watershed land cover was summarized from satellite‐derived data for the area outside a 30‐m buffer. Riparian land cover was interpreted from digital orthophotos within 10‐, 10‐to 20‐, and 20‐to 30‐m buffers. Reach‐scale habitat, fish, and macroinvertebrates were collected in 1998 and biotic indices calculated. Correlations between land cover, habitat, and stream‐quality indicators revealed significant relations at the watershed, riparian‐corridor, and reach scales. At the watershed scale, fish diversity, intolerant fish and EPT species increased, and Hilsenhoff biotic index (HBI) decreased as percent forest increased. At the riparian‐corridor scale, EPT species decreased and HBI increased as riparian vegetation became more fragmented. For the reach, EPT species decreased with embeddedness. Multivariate analyses further indicated that riparian (percent agriculture, grassland, urban and forest, and fragmentation of vegetation), watershed (percent forest) and reach‐scale characteristics (embeddedness) were the most important variables influencing fish (IBI, density, diversity, number, and percent tolerant and insectivorous species) and macroinvertebrate (HBI and EPT) communities.     

EFFECTS OF TOPOGRAPHY AND SOIL PROPERTIES ON RECHARGE AT TWO SITES IN AN AGRICULTURAL FIELD1

ABSTRACT: Field experiments were conducted from 1992 to 1995 to estimate ground water recharge rates at two sites located within a 2.7‐hectare agricultural field. The field lies in a sand plain setting in central Minnesota and is cropped continuously in field corn. The sites are located at a topographically high (upland) site and a topographically low (lowland) site in an effort to quantify the effects of depression focusing of recharge. Three site‐specific methods were used to estimate recharge rates: well hydrograph analysis, chlorofluorocarbon age dating, and an unsaturated zone water balance. All three recharge methods indicated that recharge rates at the lowland site (annual average of all methods of 29 cm) exceeded those at the upland site (annual average of 18 cm). On an annual basis, estimates by the individual methods ranged from 12 to 44 percent of precipitation at the upland site and from 21 to 83 percent at the lowland site. The difference in recharge rates between the sites is primarily attributed to depression focusing of surface water runon at the lowland site. However, two other factors were also important: the presence of thin lamellae at the upland site, and coarser textured soils below a depth of 1.5 m at the lowland site.     

Cyclodextrin‐enhanced remediation of organic and metal contaminants in porous media and groundwater

Heavy metals and toxic organic contaminants are found at numerous industrial and military sites. The generally poor performance of conventional pump‐and‐treat schemes has made the development of improved methods for contaminated site remediation a significant environmental priority. One such innovative method is cyclodextrin‐enhanced flushing of the contaminated porous media and groundwater. Cyclodextrin is a glucose‐based molecule that is produced on industrial scales by microorganisms. Over the last years, several cyclodextrin derivatives have received extensive research interest. It was shown that cyclodextrins can significantly enhance the solubility of toxic organics, and in some cases, heavy metals and radioactive isotopes. As a sugar, cyclodextrin is considered relatively non‐toxic to humans, plants, and soil microbes. Thus, there are minimal health‐related concerns associated with the injection of cyclodextrin into the subsurface, which is an inherent advantage for use of cyclodextrins as a remediation agent. This paper provides a review of the available literature concerning use of cyclodextrin for remediation of groundwater and soil.     

A simple,cost-effective multispecies toxicity test using organisms with a cosmopolitan distribution

Difficulties in making accurate, ecosystem-level predictions of environmental effects of chemicals, mixtures, and effluents based solely on the results of tests on single species have necessitated the development of more environmentally realistic, predictive testing methods. This paper describes a multispecies, community-level toxicity test based on the colonization of artificial substrates by microbial species. Tests examined the colonization of initially barren polyurethane foam artificial substrates by Protozoa from a species source colonized in a natural system. Differences in colonization were examined in microecosystems amended with low levels of cadmium, a very toxic heavy metal, and TFM, an organic biocide used against larval sea lamprey. Tests examined differences in colonization over 28 days. For cadmium, effect levels were estimated to be near 1 g 1^–1, in the low range of effect levels determined from chronic single species tests. For TFM, effect levels were estimated to be between 1 and 10 ppm, overlapping the concentrations used in environmental applications. The colonization response, which depends on naked microbial cells reproducing and migrating through toxicant amended water to new substrates, is very sensitive. Tests based on colonization can be adapted to use species from a target receiving system or can use species from a designated natural source. Field validation of these tests can employ nearly identical methods to those used in laboratory studies to assess the accuracy of predictions based on test system data.     

Limitations of an optimum sustainable population or potential biological removal approach for conserving marine mammals: Pacific walrus case study

Decision rules are the agreed-upon points at which specific management interventions are initiated. For marine mammal management under the U.S. Marine Mammal Protection Act (MMPA), decision rules are usually based on either a numeric population or biological-removal approach. However, for walrus and other ice-associated pinnipeds, the inability to reliably assess population numbers or biological removals highlights a significant gap in the MMPA, particularly when the Arctic environment is rapidly changing. We describe the MMPA's ecosystem-based management goals, and why managers have bypassed these goals in favor of an approach that depends upon numerical population assessment. We then revisit the statute's primary goals in light of current knowledge about the Pacific walrus ecosystem and new developments in environmental governance. We argue that to monitor and respond to changes in the walrus ecosystem, decision rules should be based on scientific criteria that depend less on the currently-impractical goal of accurately enumerating population size and trends, or removals from that population. Rather, managers should base decisions on ecological needs and observed ecological changes. To implement this approach would require an amendment to the MMPA that supports filling the gap in management with achievable decision rules. Alternatively, walrus and other ice-associated pinnipeds will remain largely unmanaged during a period of profound environmental change.