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71.
Runoff from sloping landscapes cropped with established alfalfa (Medicago sativa L.) may contain bioavailable P (BAP) which accelerates eutrophication of surface water bodies. Such BAP exists as either dissolved reactive P (DRP) or bioavailable reactive particulate P (BPP). We hypothesized that before and after harvest, sod-forming smooth bromegrass (Bromus inermis Leyss.) or alfalfa-smooth bromegrass mixtures would have less BAP, DRP, and BPP runoff losses than taprooted alfalfa. Swards established in 1992 near Lancaster, WI were subjected to a 72 mm simulated rainfall applied for 1 h in 1993 and 1994 to forage regrowth at 4 and 6 wk after first harvest and immediately (0 wk) after second harvest. Hourly BAP losses for all sward types were 82% less when 1.5 Mg ha(-1) of forage dry matter was present. High DRP losses (>0.050 kg ha(-1)) were associated with high DRP concentrations (>7.1 micromol L(-1)) and high surface soil P concentrations (>59 mg kg(-1)) resulting from broadcast maintenance P fertilizer. High BPP losses (>0.035 kg ha(-1)) were associated with high runoff volumes (>24 mm) and sediment concentrations (>2 g L(-1)). Summed over all 6 rainfall simulations, total BAP loss was only 0.07 kg ha(-1) at the 6 wk stage of regrowth compared with 0.35 at 4 wk, and 0.41 at 0 wk. Moreover, there was no significant difference between sward types for DRP concentration, DRP loss, or BAP loss. We conclude that avoiding excessive defoliation was more effective at reducing BAP losses than specific forage species selection.  相似文献   
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Since highly pathogenic avian influenza virus H5N1 emerged in 1997, avian influenza is considered one of the most important infectious diseases globally. In respect of virus transmission to humans, the consumption of raw poultry products remains of serious concern. In this study, data about survival time and inactivation kinetics of two low pathogenic avian influenza virus (AIV) strains (H3N8, H5N6) in short fermented raw sausage were obtained. In addition, the impact of the preserving factors d,l-lactic acid and sodium chloride on virus infectivity was evaluated through in vitro studies. Virus infectivity was confirmed in embryonated chicken eggs. Inactivation of H3N8 was seen in d,l-lactic acid solutions (0.15 and 0.20%, pH 4.40–4.70 and pH 3.80–3.91) at both temperatures (20 vs. 4°C) during 3 days of exposure. However, infectious virus particles could still be detected after exposure to 0.1% d,l-lactic acid (pH 5.80–5.99). In all NaCl solutions (2, 6 and 12% w/v), infectivity of the H3N8 strain decreased steadily but reduction of the virus titre increased significantly with higher temperature. In raw sausages, decline in virus titre was observed for both strains during ripening and storage. Thereby, decline of virus infectivity was dependent on time and temperature with a more marked effect at higher temperatures (22 vs. 7°C). At refrigeration (7°C), both viruses maintained infectivity over 14 days. Results indicate that appropriate processing of short fermented raw poultry sausage is likely to reduce risk of virus exposure due to adequate inactivation of AIV during ripening and storage.  相似文献   
74.
Microarthropods are known as vectors for soil microorganisms, predominantly fungi. This laboratory study uses the widespread unicellular green algae Chlorella vulgaris as model to assess the role of Collembola in algal dispersal and to determine the effects of gut passage on propagation. Living algal cells were observed in 70 % of the faecal pellets of Folsomia candida, Heteromurus nitidus and Protaphorura fimata. Moreover, marker fatty acids for green algae, i.e. 16:2ω6,9 and 16:3ω3,6,9, were consistently detected in the pellets. Compared to the algal diet, the high content of methyl-branched total lipid fatty acid (TLFA) with hydroxyl substitution indicated microbial colonisation during gut passage. The TLFA profile of faeces revealed no species-specific differences but similar changes in microbial communities over the duration of feeding, indicating comparable indigenous bacteria and colonisation mechanisms during gut passage. In sum, faecal pellets of soil microarthropods such as Collembola can act as a vector for both dietary algae and specific gut-associated microorganisms, with the latter likely involved in resource degradation inside and outside the gut habitat.  相似文献   
75.
Despite its successes, the U.S. Endangered Species Act (ESA) has proven challenging to implement due to funding limitations, workload backlog, and other problems. As threats to species survival intensify and as more species come under threat, the need for the ESA and similar conservation laws and policies in other countries to function efficiently has grown. Attempts by the U.S. Fish and Wildlife Service (USFWS) to streamline ESA decisions include multispecies recovery plans and habitat conservation plans. We address species status assessment (SSA), a USFWS process to inform ESA decisions from listing to recovery, within the context of multispecies and ecosystem planning. Although existing SSAs have a single-species focus, ecosystem-based research can efficiently inform multiple SSAs within a region and provide a foundation for transition to multispecies SSAs in the future. We considered at-risk grassland species and ecosystems within the southeastern United States, where a disproportionate number of rare and endemic species are associated with grasslands. To initiate our ecosystem-based approach, we used a combined literature-based and structured World Café workshop format to identify science needs for SSAs. Discussions concentrated on 5 categories of threats to grassland species and ecosystems, consistent with recommendations to make shared threats a focus of planning under the ESA: (1) habitat loss, fragmentation, and disruption of functional connectivity; (2) climate change; (3) altered disturbance regimes; (4) invasive species; and (5) localized impacts. For each threat, workshop participants identified science and information needs, including database availability, research priorities, and modeling and mapping needs. Grouping species by habitat and shared threats can make the SSA process and other planning processes for conservation of at-risk species worldwide more efficient and useful. We found a combination of literature review and structured discussion effective for identifying the scientific information and analysis needed to support the development of multiple SSAs. Article impact statement: Species status assessments can be improved by an ecosystem-based approach that groups imperiled species by shared habitats and threats.  相似文献   
76.
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This work has been supported by the Centre National de la Recherche Scientifique (C.N.R.S.) through the Action Thématique Programmée Matières Organiques des sols. We thank Dr. B. Guillet and Prof. B. Souchier, Centre de Pédologie Biologique du C.N.R.S., Nancy, for helpful discussions and advice in collecting the samples; we further thank E. Krempp and P. Wehrung for technical assistance in NMR and MS measurements.  相似文献   
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79.
Rhizosphere bacteria influence plant growth through several mechanisms. Beneficial interactions are often difficult to identify and isolate for study, and therefore favorable effects on plant productivity are not easily described in quantitative terms. Major beneficial activities of soil bacteria include solubilization of minerals, fixation of nitrogen, production of growth-promoting hormones and competitive suppression of pathogens. Most recent research to improve crop responses has emphasized the study of nitrogen-fixing bacteria indigenous to rhizospheres of cereal crops and other grasses. The amount of nitrogen available to crops from fixation by these organisms is significant under some circumstances, but efforts to control and increase fixation activity have not been consistently successful. Despite considerable research, inoculation experiments frequently fail to improve rates of nitrogen fixation. However, substantial progress has been achieved in understanding mechanisms involved in plant—bacterial interactions, and in defining conditions which control them. Also, recent studies show clearly that bacterial inoculation can be highly beneficial even when nitrogen fixation is not affected. Further research is needed to develop new and effective methods for suppressing growth of competing microorganisms, and promoting dominance of those most useful to plants. Environmental manipulation may not be sufficient to achieve this objective. Genetic development of both plant and bacterial lines which enhance desired interactions is likely to be the most productive research effort for the future.  相似文献   
80.
Karl H  Ruoff U  Blüthgen A 《Chemosphere》2002,49(7):765-773
In 1995-1998 the contents of polychlorinated dibenzodioxins and dibenzofurans of 184 pooled fish samples were analysed. Sampling focused on fish and fishery products with a market share of more than 1% and covered all fishing grounds important for the supply of the German market. Investigation included 15 different fish species, shrimp, mussel and squid samples and various fishery products, typically on the German market. Generally lean fish species like cod, saithe or Alaska pollock were less contaminated on fresh weight basis than fat fish species like herring, Greenland halibut and sardine. In herring the dioxin content is related to the fishing ground. Low concentrations were found in North Sea herring, high concentrations measured in samples from the Baltic Sea. Dioxin contents in fishery products did not differ significantly from the raw fish samples. Results allow an estimation of the daily intake of dioxins and furans via fish consumption in Germany. Based on a daily fish consumption of 20 g the average intake of dioxins via fish is 6.2 pg WHO-PCDD/F-TEQs per person and day.  相似文献   
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