Effects of salinity on the clam <Emphasis Type="Italic">Chamelea gallina</Emphasis> haemocytes. Part II: Superoxide dismutase response

The effects of different salinity levels (28, 34 and 40‰) on functional responses of Chamelea gallina haemocytes were evaluated in a two part study dealing with modulations of immune parameters. This part (Part II) of the study was focused on the superoxide dismutase (SODs) activity and expression in haemocyte lysate and cell-free haemolymph. Results of this study established that the exposure of C. gallina specimens at 40‰ salinity provoked a decrease in Mn-SOD and Cu/Zn-SOD activities in haemocyte lysate suggesting a declining superoxide anion generation at the highest salinity tested. Expression of MnSOD was coherent with activity values, while Cu/ZnSOD showed two immunoreactive bands. The former corresponds to the cytosolic Cu/Zn-SOD (16 kDa) was not coherent with the enzyme activity and the second (28–30 kDa) probably attributed to EC-SOD. In cell-free haemolymph, Mn-SOD activity decrease and Cu/Zn-SOD activity increase at 40‰ were observed, likely due to EC-SOD contribution, strongly induced at the same salinity. After EC-SOD detection with two different antibody, we postulate that the EC-SOD like-protein band (29 kDa) may be constitute partly by EC-SOD and probably by Cu/Zn-SOD dimeric form not completely dissociated under reducing condition or a Cu/Zn-SOD degenerated but still recognized by antibody. The cell-free haemolymph increase of EC-SOD at high salinity values plays an important role in immune defence of C. gallina. According to the conclusion of Part I of this study, our data confirmed the destabilizing effect of 40‰ salinity on haemocyte functionality, while to 28‰ exposure, data don’t confirm its stressful action as instead stated by results of Part I. Further studies are necessary to clear up this discrepancy.     

Effects of salinity on the clam <Emphasis Type="Italic">Chamelea gallina</Emphasis>. Part I: alterations in immune responses

In the present study, the effects of differing salinities on some important functional responses of haemocytes from the clam, Chamelea gallina, were investigated. The animals were kept for 7 days at 28‰ (hyposalinity), 34‰ (control) and 40‰ salinity (hypersalinity), and total haemocyte count (THC), haemocyte volume, phagocytosis, lysozyme-like activity (in both haemocyte lysate and cell-free haemolymph) were measured. The survival-in-air test was also performed. Clams kept at 28‰ showed significantly increased THC with respect to animals kept at 34 and 40‰. The analysis of haemocyte size frequency distribution highlighted that in clams kept at 28‰ the haemocyte fraction of about 5 μm in diameter and 50–100 femtolitre in volume increased markedly. Conversely, in animals kept at 40‰ an increase was observed in the haemocyte fraction having about 8–10 μm diameter and 400–500 femtolitre volume. Higher phagocytic activity was recorded in haemocytes from control clams, with respect to that of clams kept at 28 and 34‰. Lysozyme-like activity in haemocyte lysate was shown to increase significantly in animals kept at 28‰ with respect to that of clams kept at 40‰, whereas enzyme activity in cell-free haemolymph from clams kept at 34‰ was significantly higher with respect to that of clams maintained at 40‰. A relationship between phagocytosis and lysozyme secretion is suggested. The resistance to air exposure of clams kept at 28 and 40‰ was shown to decrease significantly; LT₅₀ values fell from 7 days in clams kept at 34‰ to 4 and 5 days in those kept at 28 and 40‰, respectively. Results demonstrated that salinity values far from 34‰ affects the functional responses of haemocytes and reduce the resistance of clams to exposure to air.     

PRECIPITATION RETENTION AND SOIL EROSION UNDER VARYING CLIMATE,LAND USE,AND TILLAGE AND CROPPING SYSTEMS1

ABSTRACT: Nonirrigated crop yields and forage production are limited by low and variable precipitation in the southern Great Plains. Precipitation variation involves production risks, which can be reduced by considering probability of precipitation, precipitation retention, and soil erosion under various production systems. The objective of this study was to probabilistically quantify the impact of precipitation variations, land use, cropping, and tillage systems on precipitation retention and soil erosion. Five 1.6 ha watersheds that had 3 to 4 percent slopes, and similar silt loam soils were selected. One was kept in native grass, and the others were planted into winter wheat (Triticum aestivum L.) under different cropping and tillage systems. Daily runoff and soil erosion were measured at the outlet of each watershed. Precipitation distributions exhibited great seasonal and interannual variations, and precipitation retention distributions resembled those of precipitation. Cropping and tillage systems affected precipitation retention but much less than did precipitation variations. Available soil water storage, which was largely controlled by ET, played an important role in retaining precipitation. This indicates that cropping systems should be adjusted to precipitation patterns, if predictable, for better soil water use. Land use and cropping and tillage systems had a much greater impact on soil erosion than on precipitation retention. Soil erosion risks, which were proportional to the levels of tillage disturbance, were mainly caused by a few large storms in summer, when surface cover was low. This study explored a novel approach for evaluating production risks associated with insufficient precipitation retention and excessive soil erosion for certain crops or cropping systems under assumed precipitation conditions.     

AUTOMATED EXTRACTION OF DRAINAGE NETWORK AND WATERSHED DATA FROM DIGITAL ELEVATION MODELS1

ABSTRACT: This paper discusses a computer program which extracts a number of watershed and drainage network properties directly from digital elevation models (DEM) to assist in the rapid parameterization of hydrologic runoff models. The program integrates new and established algorithms to address problems inherent in the analysis low-relief terrain from raster DEMs similar to those distributed by the U.S. Geological Survey for 7.5-minute quadrangles. The program delineates the drainage network from a DEM, and determines the Strahler order, total and direct drainage area, length, slope, and upstream and downstream coordinates of each channel link. It also identifies the subwatershed of each channel source and of the left and right bank of each channel link, and assigns a unique number to each network node. The node numbers are used to associate each subwatershed with the channel link to which it drains, and can be used to control flow routing in cascade hydrologic models. Program output includes tabular data and raster maps of the drainage network and subwatersheds. The raster maps are intended for import to a Geographical Information System where they can be registered to other data layers and used as templates to extract additional network and subwatershed information.     

Swimming obstructed by dead-water

In nautical literature, ‘dead-water’ refers to the obstructive effect encountered by ships moving in stratified water due to the ship generating waves on an interface that separates different water masses. To investigate the hypothesis that open water swimming may also be obstructed by an encounter of dead-water, possibly causing drowning, we performed two experiments that assess the impact of stratified water on swimming. In the first experiment, subjects made a single front-crawl stroke while lying on a carriage that was rolling just above the water surface. The gain in kinetic energy, as a result of the stroke, was far less in stratified than in homogeneous water. In the second experiment, four subjects swam a short distance (5 m) in homogeneous and in two different settings of stratified water. At the same stroke frequency, swimming in stratified conditions was slower by 15%, implying a loss in propulsive power by 40%. Although in nature stratification will be less strong, extrapolation of the results suggests that dead-water might indeed obstruct swimming in open water as well. This effect will be most pronounced during fair weather, when stratification of a shallow surface layer is most easily established. Our findings indicate that swimmers’ anecdotal evidence on ‘water behaving strangely’ may have to be taken more seriously than previously thought.     

Design and field application of an automated cartridge sampler for VOC concentration and flux measurements

One of the major limitations in advancing the understanding of tropospheric ozone and aerosol generation and developing strategies for their control is the technical ability to accurately measure volatile organic compounds (VOCs). This paper describes the design of a constant flow VOC sampler. The versatile sampler can be used for fully automated concentration and flux measurements of VOCs. The sampler incorporates a microprocessor control unit and provides highly accurate mass flow control and significant ease of operation. Sampling sequences can be programmed directly or by remote control through a PC. All important operational parameters necessary for a complete sampling audit trail are logged. Compact weatherproof housings and low power consumption allow operation at remote sites and locations which are sensitive to disturbances or have restricted access. Inner wetted surfaces of the sampler are constructed from non-contaminating materials that do not sorb or emit VOC, and thus permit the collection of representative samples even in environments with very low VOC concentrations. The cartridge magazine provides a maximum of 20 sequential cartridge samples, which allows for long-term air quality assessments. In the dual channel mode, two samples can be collected simultaneously through two independent sample loops, providing ten sequential sample pairs. This design allows the parallel collection of (a) quality assurance backup samples, (b) samples on two different types of cartridges/sorbents to allow a variety of analyses, or (c) differential samples for flux measurements using enclosure, aerodynamic profile, or relaxed eddy accumulation (REA) methods. Field applications including airplane profile measurements above a tropical rainforest area, as well as gradient and REA measurements over a mid-latitude mixed forest stand are described, and demonstrate the validity and flexibility of the system. In particular, the application of the VOC sampler as an integrated part of a REA system is emphasized.     

HYDROLOGIC SIMULATION OF THE LITTLE WASHITA RIVER EXPERIMENTAL WATERSHED USING SWAT1

ABSTRACT: Precipitation and streamflow data from three nested subwatersheds within the Little Washita River Experimental Watershed (LWREW) in southwestern Oklahoma were used to evaluate the capabilities of the Soil and Water Assessment Tool (SWAT) to predict streamflow under varying climatic conditions. Eight years of precipitation and streamflow data were used to calibrate parameters in the model, and 15 years of data were used for model validation. SWAT was calibrated on the smallest and largest sub‐watersheds for a wetter than average period of record. The model was then validated on a third subwatershed for a range in climatic conditions that included dry, average, and wet periods. Calibration of the model involved a multistep approach. A preliminary calibration was conducted to estimate model parameters so that measured versus simulated yearly and monthly runoff were in agreement for the respective calibration periods. Model parameters were then fine tuned based on a visual inspection of daily hydrographs and flow frequency curves. Calibration on a daily basis resulted in higher baseflows and lower peak runoff rates than were obtained in the preliminary calibration. Test results show that once the model was calibrated for wet climatic conditions, it did a good job in predicting streamflow responses over wet, average, and dry climatic conditions selected for model validation. Monthly coefficients of efficiencies were 0.65, 0.86, and 0.45 for the dry, average, and wet validation periods, respectively. Results of this investigation indicate that once calibrated, SWAT is capable of providing adequate simulations for hydrologic investigations related to the impact of climate variations on water resources of the LWREW.     

Tundra in the rain: differential vegetation responses to three years of experimentally doubled summer precipitation in Siberian shrub and Swedish bog tundra

Precipitation amounts and patterns at high latitude sites have been predicted to change as a result of global climatic changes. We addressed vegetation responses to three years of experimentally increased summer precipitation in two previously unaddressed tundra types: Betula nana-dominated shrub tundra (northeast Siberia) and a dry Sphagnum fuscum-dominated bog (northern Sweden). Positive responses to approximately doubled ambient precipitation (an increase of 200 mm year(-1)) were observed at the Siberian site, for B. nana (30 % larger length increments), Salix pulchra (leaf size and length increments) and Arctagrostis latifolia (leaf size and specific leaf area), but none were observed at the Swedish site. Total biomass production did not increase at either of the study sites. This study corroborates studies in other tundra vegetation types and shows that despite regional differences at the plant level, total tundra plant productivity is, at least at the short or medium term, largely irresponsive to experimentally increased summer precipitation.