Effluent impact assessment using microarray-based analysis in common carp: a systems toxicology approach

Effluents are a main source of direct and continuous input of pollutants to the aquatic environment, and can cause ecotoxicological effects at different levels of biological organization. Since gene expression responses represent the primary interaction site between environmental contaminants and biota, they provide essential clues to understand how chemical exposure can affect organismal health. The aim of the present study was to investigate the applicability of a microarray approach for unraveling modes of action of whole effluent toxicity and impact assessment. A chronic toxicity test with common carp (Cyprinus carpio) was conducted where fish were exposed to a control and 100% effluent for 21 days under flow-through conditions. Microarray analysis revealed that effluent treatment mainly affected molecular pathways associated with the energy balance of the fish, including changes in carbohydrate and lipid metabolism, as well as digestive enzyme activity. These gene expression responses were in clear agreement with, and provided additional mechanistic information on various cellular and higher level effects observed for the same effluent. Our results demonstrate the benefit of toxicogenomic tools in a “systems toxicology” approach, involving the integration of adverse effects of chemicals and stressors across multiple levels of biological complexity.     

MONTHLY RUNOFF PREDICTIONS BASED ON RAINFALL FORECASTS IN A SMALL OKLAHOMA WATERSHED1

ABSTRACT: Conditions under which monthly rainfall forecasts translate into monthly runoff predictions that could support water resources planning and management activities were investigated on a small watershed in central Oklahoma. Runoff response to rainfall forecasts was simulated using the hydrologic model SWAT. Eighteen scenarios were examined that represented combinations of wet, average, and dry antecedent rainfall conditions, with wet, normal, and dry forecasted rainfall. Results suggest that for the climatic and physiographic conditions under consideration, rainfall forecasts could offer potential application opportunities in surface water resources but only under certain conditions. Pronounced wet and dry antecedent rainfall conditions were shown to have greater impact on runoff than forecasts, particularly in the first month of a forecast period. Large forecast impacts on runoff occurred under wet antecedent conditions, when the fraction of forecasted rainfall contributing to runoff was greatest. Under dry antecedent conditions, most of the forecasted rainfall was absorbed in the soil profile, with little immediate runoff response. Persistent three‐month forecasts produced stronger impacts on runoff than one‐month forecasts due to cumulative effects in the hydrologic system. Runoff response to antecedent conditions and forecasts suggest a highly asymmetric utility function for rainfall forecasts, with greatest decision‐support potential for persistent wet forecasts under wet antecedent conditions when the forecast signal is least dampened by soil‐storage effects. Under average and dry antecedent conditions, rainfall forecasts showed little potential value for practical applications in surface water resources assessments.     

Emergency medical preparedness during the 2006 World Cup in Frankfurt, Germany

This paper describes emergency medical preparedness during FIFA (Fédération Internationale de Football Association) World Cup matches in Frankfurt, Germany, in 2006. The methods employed were document analysis and personal observation of games over five days in June–July 2006. The medical authorities in Frankfurt drew on a wide range of scientific literature and experiences to elaborate a National Concept. They paid attention to different models of handling mass catastrophes in shaping the final version of the document. The participation of designated authorities, associations, and volunteer organisations was coordinated sufficiently and the games in Frankfurt proceeded without great incident, even though more than 300,000 people in total attended. The adopted emergency medical procedure was appropriate for a mass gathering event. Official and volunteer organisations collaborated precisely in emergency preparedness. While one uniform concept for all mass gatherings events cannot be developed, case reports and experiences are useful tools.