Comparison of PCDD/F and coplanar PCB concentrations in Japanese human adipose tissue collected in 1970-1971, 1994-1996 and 2000

Concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and coplanar biphenyls (Co-PCBs) were determined in Japanese human adipose tissues. Temporal trends were assessed by comparing data collected during 1970-1971, 1994-1996 and 2000. Mean TEQ levels of PCDD/Fs in human adipose tissue showed a significant decrease from 31.6 +/- 9.2 pg-TEQ/g fat wt in 1970-1971 to 11.9 +/- 7.4 pg-TEQ/g fat wt in 2000, and coplanar PCBs decreased from 35.4 +/- 21.9 pg-TEQ/g fat wt in 1970-1971 to 15.3 +/- 8.2 pg-TEQ/g fat wt in 2000. However, some PCB congener concentrations did not change during this time. A comparison of data using the latest PCB-TEQ showed no significant changes. Therefore, research on source characterization and monitoring of short-term temporal trends on these compounds should be carried out continuously.     

Dechlorination of hexachlorobiphenyl by using potassium-sodium alloy

2,2',4,4',5,5'-Hexachlorobiphenyl (HCB) was dechlorinated with potassium-sodium (K-Na) alloy under an inert gas atmosphere. Solvent effect was observed in the reaction. Dechlorination yields in benzene and cyclohexane were 99.9998%, and 99.99996%, respectively. The reaction was exothermic and proceeded at room temperature. In benzene, trace amounts of polychlorinated biphenyls (PCBs) as products by stepwise dechlorination and polychlorinated quarterphenyls as product of Wurtz-Fittig reaction were detected as reaction intermediate. Reaction products were biphenyl, cyclohexylbenzene, and dicyclohexyl. In cyclohexane, there were no products of Wurtz-Fittig reaction. Dechlorination at para-position preferred to that at ortho-position, judging from analysis of PCBs as intermediates of stepwise dechlorination.     

Environmental fate of pesticides used in Australian viticulture V. Behaviour of atrazine in the soils of the south Australian Riverland

The transport of the s‐triazine herbicide, atrazine, through the red, calcareous earth soils of the South Australian Riverland was investigated. Small, undisturbed soil cores were extracted from the inter‐row topsoil of a vineyard adjacent to the River Murray, approximately 10 km south‐west of Overland Corner, South Australia. The vines were grown in a deep (1–4 m) reddish brown, strongly alkaline, sandy loam with a low organic carbon content (<2%). Atrazine concentrations in the leachate were dependent on application rate and soil type. High application rates on subsoil gave high rates of leaching for a longer time compared to the same application rate on topsoil and/or lower application rates on either topsoil or subsoil. Overall, 37–65% of the applied atrazine was detected in the leachate from subsoil cores, 14–25% in topsoil core leachates. Small amounts of atrazine (< 10% of applied dose) were found only in the top 2 cm of the core profiles. The results suggest that this herbicide is somewhat mobile in such strongly alkaline, sandy loam soils and that the irrigated soils of this region are likely to be prone to leaching of atrazine, and therefore that groundwater supplies in this area may be at risk of contamination through use of triazine herbicides.     

Emissions Scenarios Database and Review of Scenarios

This paper reviews and analyzes more than 400 scenarios of global and regional greenhouse gas emissions and their main driving forces - population, economy, energy intensity, and carbon intensity - drawn from an extensive literature survey and summarized in a database. This new and growing database is available online, which makes summary statistics on these scenarios widely available. The scenarios in the database were collected from almost 200 different literature sources and other scenario evaluation activities. The ultimate objective of the database is to include all relevant global and regional emissions scenarios. This paper shows how the database can be utilized for the analysis of greenhouse gas emissions ranges across the scenarios in the literature and for the analysis of their main driving forces. The scenarios in the database display a large range of future greenhouse gas emissions. Part of the range can be attributed to the different methods and models used to formulate the scenarios, which include simple spreadsheet models, macroeconomic models and systems-engineering models. However, most of the range is due to differences in the input assumptions for the scenarios, in particular of the main scenario driving forces. Special emphasis is given to an analysis of medians and ranges of scenario distributions and the distributions of the main scenario driving forces in the database. The analysis shows that the range for projected population increase in the world, across the scenarios in the database, is the smallest of all main driving forces (about a factor of 3 in 2100). The range of economic growth, measured by the gross world product, and the range of primary energy consumption vary by a factor of 10 in 2100. Carbon intensity of energy, an indicator of the degree of technological change, varies by nearly two orders of magnitude in the year 2100. In addition, this paper presents the first attempt to analyze the relationships among the main scenario driving forces. Subsequent papers in this special issue give further analyses of the relationships among the main scenario driving forces and their other relevant characteristics.     

Verification of lipofuscin-based crustacean ageing: seasonality of lipofuscin accumulation in the stomatopod Oratosquilla oratoria in relation to water temperature

To verify the lipofuscin-based age estimation of crustaceans of unknown age from wild populations, we conducted a modal analysis of lipofuscin-concentration histograms in the stomatopod Oratosquilla oratoria seasonally collected from Tokyo Bay, Japan. Confocal microscopy and subsequent image analysis were used to quantify lipofuscin concentrations in the protocerebral bridge cell mass in the brain. Conventional body length frequency analysis failed to detect modes corresponding to ≥1 year old. In contrast, four or five distinct modes were found in the lipofuscin-concentration histograms, in which modes could be readily traced across months, suggesting that each mode represented an independent age group. The lipofuscin accumulation rate fluctuated seasonally: it was high in summer (June–July) and low in winter (December–January). Cross-correlation analysis showed that the highest instantaneous lipofuscin accumulation rate was achieved in early summer before maximum bottom temperatures were reached and when the temperature was increasing most rapidly. Our findings support the usefulness of lipofuscin concentration analysis in age estimation in wild crustacean populations.     

Starvation-induced barotolerance as a survival mechanism of a psychrophilic marine vibrio in the waters of the Antarctic convergence

Ant-300, a psychrophilic marine vibrio isolated from the Antarctic convergence, was tested for survival under conditions simulating those expected in situ for this organism. In particular, the organism's response to increased hydrostatic pressure was examined. Under a hydrostatic pressure of 250 atm, the viability of Ant-300 was reduced over 755 in growth media and under starvation conditions after 3 and 2 days, respectively. However, if the cells were starved for 1 week prior to pressurization of the starving suspension, 100% viability was maintained for over 6 weeks at the same pressure. After 10 weeks, the viability of a population of cells suspended for 1 week in natural or artificial seawater at a density of 10³ cells ml^–1 prior to pressurization of the suspension was 2 to 3 times greater than 1 atm controls. The data indicate that starvation conditions are a contributory if not the primary factor for the barotolerance of this organism in Antarctic waters. The data also indicate that under certain conditions, not unlike those expected in situ, hydrostatic pressure actually increases the survival of this organism under low nutrient conditions.Technical Paper No. 4907, Oregon Agricultural Experiment Station.     

Levels of 3,3′,4,4′-tetrachloroazobenzene in herbicides and bulk reagents

The contamination levels of different commercial herbicides and bulk reagents with the carcinogen 3,3′,4,4′-tetrachloroazobenzene (TCAB) were determined by high resolution mass spectrometry. The concentrations vary from 0.00071 to 2.8 ug/g (ppm) for the herbicides and from 0.085 to 14 ug/g for the bulk reagents. They are thus lower than those of other reports.