Evaluation of a combined macrophyte–epiphyte bioassay for assessing nutrient enrichment in the Portneuf River,Idaho, USA

We describe and evaluate a laboratory bioassay that uses Lemna minor L. and attached epiphytes to characterize the status of ambient and nutrient-enriched water from the Portneuf River, Idaho. Specifically, we measured morphological (number of fronds, longest surface axis, and root length) and population-level (number of plants and dry mass) responses of L. minor and community-level (ash-free dry mass [AFDM] and chlorophyll a [Chl a]) responses of epiphytes to nutrient enrichment. Overall, measures of macrophyte biomass and abundance increased with increasing concentrations of dissolved phosphorus (P) and responded more predictably to nutrient enrichment than morphological measures. Epiphyte AFDM and Chl a were also greatest in P-enriched water; enrichments of N alone produced no measurable epiphytic response. The epiphyte biomass response did not directly mirror macrophyte biomass responses, illustrating the value of a combined macrophyte–epiphyte assay to more fully evaluate nutrient management strategies. Finally, the most P-enriched waters not only supported greater standing stocks of macrophyte and epiphytes but also had significantly higher water column dissolved oxygen and dissolved organic carbon concentrations and a lower pH. Advantages of this macrophyte–epiphyte bioassay over more traditional single-species assays include the use of a more realistic level of biological organization, a relatively short assay schedule (~10 days), and the inclusion of multiple biological response and water-quality measures.     

Quantifying sample biases of inland lake sampling programs in relation to lake surface area and land use/cover

We quantified potential biases associated with lakes monitored using non-probability based sampling by six state agencies in the USA (Michigan, Wisconsin, Iowa, Ohio, Maine, and New Hampshire). To identify biases, we compared state-monitored lakes to a census population of lakes derived from the National Hydrography Dataset. We then estimated the probability of lakes being sampled using generalized linear mixed models. Our two research questions were: (1) are there systematic differences in lake area and land use/land cover (LULC) surrounding lakes monitored by state agencies when compared to the entire population of lakes? and (2) after controlling for the effects of lake size, does the probability of sampling vary depending on the surrounding LULC features? We examined the biases associated with surrounding LULC because of the established links between LULC and lake water quality. For all states, we found that larger lakes had a higher probability of being sampled compared to smaller lakes. Significant interactions between lake size and LULC prohibit us from drawing conclusions about the main effects of LULC; however, in general lakes that are most likely to be sampled have either high urban use, high agricultural use, high forest cover, or low wetland cover. Our analyses support the assertion that data derived from non-probability-based surveys must be used with caution when attempting to make generalizations to the entire population of interest, and that probability-based surveys are needed to ensure unbiased, accurate estimates of lake status and trends at regional to national scales.     

Using a relative health indicator (RHI) metric to estimate health risk reductions in drinking water

When a new drinking water regulation is being developed, the USEPA conducts a health risk reduction and cost analysis to, in part, estimate quantifiable and non-quantifiable cost and benefits of the various regulatory alternatives. Numerous methodologies are available for cumulative risk assessment ranging from primarily qualitative to primarily quantitative. This research developed a summary metric of relative cumulative health impacts resulting from drinking water, the relative health indicator (RHI). An intermediate level of quantification and modeling was chosen, one which retains the concept of an aggregated metric of public health impact and hence allows for comparisons to be made across “cups of water,” but avoids the need for development and use of complex models that are beyond the existing state of the science. Using the USEPA Six-Year Review data and available national occurrence surveys of drinking water contaminants, the metric is used to test risk reduction as it pertains to the implementation of the arsenic and uranium maximum contaminant levels and quantify “meaningful” risk reduction. Uranium represented the threshold risk reduction against which national non-compliance risk reduction was compared for arsenic, nitrate, and radium. Arsenic non-compliance is most significant and efforts focused on bringing those non-compliant utilities into compliance with the 10 μg/L maximum contaminant level would meet the threshold for meaningful risk reduction.     

Fresh, stockpiled, and composted beef cattle feedlot manure: nutrient levels and mass balance estimates in Alberta and Manitoba

The fate of manure nutrients in beef cattle (Bos taurus) feedlots is influenced by handling treatment, yet few data are available in western Canada comparing traditional practices (fresh handling, stockpiling) with newer ones (composting). This study examined the influence of handling treatment (fresh, stockpiled, or composted) on nutrient levels and mass balance estimates of feedlot manure at Lethbridge, Alberta, and Brandon, Manitoba. Total carbon (TC) concentration of compost (161 kg Mg(-1)) was lower (P < 0.001) than stockpiled (248 kg Mg(-1)), which was in turn lower (P < 0.001) than fresh manure (314 kg Mg(-1)). Total nitrogen (TN) concentration was not affected by handling treatment while total phosphorus (TP) concentration increased with composting at Lethbridge. The percent inorganic nitrogen (PIN) was lower (P < 0.01) for compost (5.1%) than both fresh (24.7%) and stockpiled (28.9%) manure. Composting led to higher (P < 0.05) dry matter (DM) losses (39.8%) compared to stockpiling (22.5%) and higher (P < 0.05) total mass (water + DM) losses (65.6 vs. 35.2%). Carbon (C) losses were higher (P < 0.01) with composting (66.9% of initial) than with stockpiling (37.5%), as were nitrogen (N) losses (46.3 vs. 22.5%, P < 0.05). Composting allowed transport of two times as much P as fresh manure and 1.4 times as much P as stockpiled manure (P < 0.001) on an "as is" basis. Our study looked at one aspect of manure management (i.e., handling treatment effects on nutrient concentrations and mass balance estimates) and, as such, should be viewed as one component in the larger context of a life cycle assessment.     

Biodegradation of disodium cocoamphodiacetate by a wastewater microbial consortium.

Biodegradation of an amphoteric surfactant commonly used in personal care products, disodium cocoamphodiacetate (DSCADA), was evaluated. Results from respirometry experiments indicated that high levels of DSCADA (>216 mg/L) may be toxic to bacteria in wastewater treatment processes. Limited biodegradation, with 50% dissolved organic carbon (DOC) removal and 80% chemical oxygen demand removal was observed in batch assays, while complete removal of the parent compound, DSCADA, was noted. Oxygen biosensors were used to evaluate biodegradability of the metabolites present in the batch samples. Additional aerobic microbial activity was not detected in these samples, even with a residual DOC of approximately 45 mg/L. Results from this research indicate that biodegradability of DSCADA is limited and recalcitrant metabolites may be formed. Because DSCADA is a commonly used surfactant and is present in domestic and industrial wastewater, the associated risk posed by residual compounds should be carefully evaluated.     

Influence of Small Dams on Downstream Channel Characteristics in Pennsylvania and Maryland: Implications for the Long‐Term Geomorphic Effects of Dam Removal1

Abstract: We evaluate the effects of small dams (11 of 15 sites less than 4 m high) on downstream channels at 15 sites in Maryland and Pennsylvania by using a reach upstream of the reservoir at each site to represent the downstream reach before dam construction. A semi‐quantitative geomorphic characterization demonstrates that upstream reaches occupy similar geomorphic settings as downstream reaches. Survey data indicate that dams have had no measurable influence on the water surface slope, width, and the percentages of exposed bedrock or boulders on the streambed. The median grain diameter (D₅₀) is increased slightly by dam construction, but D₅₀ remains within the pebble size class. The percentage of sand and silt and clay on the bed averages about 35% before dam construction, but typically decreases to around 20% after dam construction. The presence of the dam has therefore only influenced the fraction of finer‐grained sediment on the bed, and has not caused other measurable changes in fluvial morphology. The absence of measurable geomorphic change from dam impacts is explicable given the extent of geologic control at these study sites. We speculate that potential changes that could have been induced by dam construction have been resisted by inerodible bedrock, relatively immobile boulders, well‐vegetated and cohesive banks, and low rates of bed material supply and transport. If the dams of our study are removed, we argue that long‐term changes (those that remain after a period of transient adjustment) will be limited to increases in the percentage of sand and silt and clay on the bed. Thus, dam removal in streams similar to those of our study area should not result in significant long‐term geomorphic changes.     

Effect of Matrix–Particle Interfacial Adhesion on the Mechanical Properties of Poly(lactic acid)/Wood-Flour Micro-Composites

The influence of interfacial matrix/particle adhesion on the mechanical properties of poly(lactic acid) (PLA) micro-composites was investigated. The tensile strength of PLA/wood-flour micro-composites is almost independent of wood-flour content, suggesting only weak adhesion exists between the PLA matrix and the wood-flour particles. The addition of wood-flour resulted in an increase of up to 95% in the tensile modulus, in comparison with pure PLA, which showed a more resilient matrix. The addition of a coupling agent, methylenediphenyl-diisocyanate (MDI) to the composition resulted in an increase in tensile strength and tensile modulus of the micro-composites, of 10 and 135%, respectively, indicating enhanced matrix–particle interfacial adhesion. SEM and electron probe microanalysis provided evidence of improved interfacial adhesion between PLA and wood-flour particles from the addition of MDI. In contrast, addition of PEAA resulted in a micro-composite displaying substantially reduced tensile strength, up to 35% and a slightly increased in impact strength, up to 15%, consistent with the introduction of the rubbery PEAA component into the polymeric matrix. No evidence for increased matrix–particle adhesion was found for the PLA/wood-flour micro-composites containing PEAA.     

Simulated effects of sulfur deposition on nutrient cycling in class I wilderness areas

We predicted the effects of sulfate (SO(4)) deposition on wilderness areas designated as Class I air quality areas in western North Carolina using a nutrient cycling model (NuCM). We used three S deposition simulations: current, 50% decrease, and 100% increase. We measured vegetation, forest floor, and root biomass and collected soil, soil solution, and stream water samples for chemical analyses. We used the closest climate stations and atmospheric deposition stations to parameterize NuCM. The areas were: Joyce Kilmer (JK), Shining Rock (SR), and Linville Gorge (LG). They differ in soil acidity and nutrients, and soil solution and stream chemistry. Shining Rock and LG have lower soil solution base cation and higher acidic ion concentrations than JK. For SR and LG, the soil solution Ca/Al molar ratios are currently 0.3 in the rooting zone (A horizon), indicating Al toxicity. At SR, the simulated Ca/Al ratio increased to slightly above 1.5 after the 30-yr simulation regardless of S deposition reduction. At LG, Ca/Al ratios ranged from 1.6 to 2.4 toward the end of the simulation period, the 100% increase scenario had the lower value. Low Ca/Al ratios suggest that forests at SR and LG are significantly stressed under current conditions. Our results also suggest that SO(4) retention is low, perhaps contributing to their high degree of acidification. Their soils are acidic, low in weatherable minerals, and even with large reductions in SO(4) and associated acid deposition, it may take decades before these systems recover from depletion of exchangeable Ca, Mg, and K.     

Changing weather and climate in Northern Ghana: comparison of local perceptions with meteorological and land cover data

Regional Environmental Change - Local perspectives on changing weather and climate and analyses of meteorological data represent two different but potentially complementary ways of knowing about...