Comparison of response of six different luminescent bacterial bioassays to bioremediation of five contrasting oils

The performance of six different bioluminescent bacteria for the assessment of oil bioremediation was compared. Three contained lux genes linked to promoters from hydrocarbon degradation pathways: Pseudomonas fluorescens HK44 (pUTK21), Escherichia coli HMS174 (pOS25) and E. coli DH5 alpha (pGEc74, pJAMA7), responding to naphthalene, isopropylbenzene and octane, respectively. The other three expressed lux constitutively: E. coli HB101 (pUCD607) and P. putida F1 (pUCD607) are genetically engineered, while Vibrio fischeri is naturally bioluminescent and was included to facilitate comparison with previous work. Five different oils (four crude oils plus diesel) were spiked into soil, and the progress of remediation was followed over a period of 119 d by monitoring both hydrocarbon disappearance and changes in the microbial response to soil extracts. The octane bioassay was the only one of the hydrocarbon-responsive bacterial assays to show any appreciable response, with up to 20-fold induction by light crude oils. Heavy crude oil and diesel elicited a much weaker response. The metabolic (lux constitutively expressed) bioassays showed that there was a general increase in toxicity over the course of the experiment, although toxicity to E. coli HB101 (pUCD607) appeared to be decreasing by the final sampling point. The metabolic bioassay response was much less variable between the different oils than for the first three, catabolic, strains.     

Evaluation of interactive toxicity of chlorophenols in water and soil using lux-marked biosensors

An assessment of the toxicity of three chlorophenol compounds (2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP)), individually and in combinations, was made in deionised water, soil extracts and soils using the lux-marked microbial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. These biosensors responded to the bioavailable fraction of pollutants enabling a rapid and ecologically relevant toxicity test. Toxicity interaction responses of pollutant mixtures were predicted after individual compounds were assessed. Synergistic interactions were observed in the response of P. fluorescens to all combinations of chlorophenols tested, while the toxicity response of E. coli varied with the matrices tested. Soil characteristics influenced the toxicity response when compared with aqueous solutions. These results highlight the significance of interactive factors and physicochemical parameters when evaluating toxicity. To develop an understanding of pollution derived hazard assessment in soils we need to integrate a wide spectrum of parameters.     

Characterization of colloidal arsenic at two abandoned gold mine sites in Nova Scotia, Canada, using asymmetric flow-field flow fractionation-inductively coupled plasma mass spectrometry

Asymmetric flow-field flow fractionation-inductively-coupled plasma-mass spectrometry was used to determine whether colloidal arsenic(As) exists in soil pore water and soil extract samples at two arsenic-contaminated abandoned gold mines(Montague and Goldenville, Nova Scotia). Colloidal arsenic was found in 12 out of the 80 collected samples(= 15%), and was primarily associated with iron(Fe) in the encountered colloids. The molar Fe/As ratios indicate that the colloids in some samples appeared to be discrete iron–arsenic minerals, whereas in other samples, they were more consistent with As-rich iron(oxy)hydroxides. Up to three discrete size fractions of colloidal As were encountered in the samples, with mean colloid diameters between 6 and 14 nm. The pore water samples only contained one size fraction of As-bearing colloids(around 6 nm diameter), while larger As-bearing colloids were only encountered in soil extracts.     

Using spatial patterns in illegal wildlife uses to reveal connections between subsistence hunting and trade

Although most often considered independently, subsistence hunting, domestic trade, and international trade as components of illegal wildlife use (IWU) may be spatially correlated. Understanding how and where subsistence and commercial uses may co‐occur has important implications for the design and implementation of effective conservation actions. We analyzed patterns in the joint geographical distribution of illegal commercial and subsistence use of multiple wildlife species in Venezuela and evaluated whether available data were sufficient to provide accurate estimates of the magnitude, scope, and detectability of IWU. We compiled records of illegal subsistence hunting and trade from several sources and fitted a random‐forest classification model to predict the spatial distribution of IWUs. From 1969 to 2014, 404 species and 8,340,921 specimens were involved in IWU, for a mean extraction rate of 185,354 individuals/year. Birds were the most speciose group involved (248 spp.), but reptiles had the highest extraction rates (126,414 individuals/year vs. 3,133 individuals/year for birds). Eighty‐eight percent of international trade records spatially overlapped with domestic trade, especially in the north and along the coast but also in western inland areas. The distribution of domestic trade was broadly distributed along roads, suggesting that domestic trade does not depend on large markets in cities. Seventeen percent of domestic trade records overlapped with subsistence hunting, but the spatial distribution of this overlap covered a much larger area than between commercial uses. Domestic trade seems to respond to demand from rural more than urban communities. Our approach will be useful for understanding how IWU works at national scales in other parts of the world.     

Observed and predicted impacts of climate change on the estuaries of south-western Australia,a Mediterranean climate region

Regional Environmental Change - Regions with a Mediterranean climate are generally predicted to become warmer and drier with climate change. Estuaries in these regions are influenced by a broad...     

Effect of metabolic uncouplers on the performance of toluene-degrading biotrickling filter

Environmental Science and Pollution Research - The biomass control potential of three metabolic uncouplers (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), carbonyl cyanide...     

INPUTS OF COPPER‐BASED CROP PROTECTANTS TO COASTAL CREEKS FROM PASTICULTURE RUNOFF1

ABSTRACT: Inputs of copper‐based crop protectants from tomato fields grown under plastic mulch agriculture (plasticulture) to an estuarine creek were investigated. Copper was measured in runoff from diverse land‐uses including conventional agriculture, plasticulture, residences, and natural areas. Water column and sediment copper concentrations were measured in plasticulture and control (nonagriculture) watersheds. Copper concentrations in plasticulture‐impacted creeks exceeded background levels episodically. High concentrations occurred during or immediately after runoff‐producing rains. Concentrations of 263 μg/L total copper and 126 μg/L dissolved copper were measured in a tidal creek affected by plasticulture; concentrations exceeded the shellfish LC₅₀ values and the water quality criteria of 2.9 μg/L dissolved copper. Control watersheds indicated background water column levels of ≤ 4 μg/L dissolved copper with similar copper levels during periods with and without rain. The copper concentrations in tomato plasticulture field runoff itself contained up to 238 μg/L dissolved copper. Copper concentrations in runoff from other land‐uses were less than 5 μg/L dissolved copper. Creek sediment samples adjacent to a plasticulture field contained significantly higher copper concentrations than sediments taken from nonplasticulture watersheds.     

Freeze/thaw durability of concrete with recycled demolition aggregate compared to virgin aggregate concrete

An increasing trend towards the use of sustainable processes has led to recycled materials being incorporated into concrete. It is generally recognised that crushed recycled construction aggregate material produces concrete of a lower compressive strength, when used as an aggregate replacement due to deleterious materials within the aggregate.It is generally accepted that in the UK, freeze/thaw attack is, after chloride-induced corrosion, the most common cause of concrete deterioration. This paper addresses the freeze/thaw durability of recycled aggregate concrete using a paired comparison test based upon weight loss and final compressive strength.Recycled aggregate concrete was found to be of at least equal durability to concrete manufactured with virgin aggregates. This was due to careful selection of the replacement aggregate and treatment prior to batching.Durability is an important material property and recycled aggregates need to be widely tested to gain confidence for use within the industry and this work shows future possibilities.     

Linking biosensor responses to Cd, Cu and Zn partitioning in soils

Soils bind heavy metals according to fundamental physico-chemical parameters. Bioassays, using bacterial biosensors, were performed in pore waters extracted from 19 contrasting soils individually amended with Cd, Cu and Zn concentrations related to the EU Sewage Sludge Directive. The biosensors were responsive to pore waters extracted from Zn amended soils but less so to those of Cu and showed no toxicity to pore water Cd at these environmentally relevant amended concentrations. Across the range of soils, the solid-solution heavy metal partitioning coefficient (K(d)) decreased (p<0.01) with increasing amendments of Cu and Zn; Cu exhibited the highest K(d) values. Gompertz functions of Cu and Zn, K(d) values against luminescence explained the relationship between heavy metals and biosensors. Consequently, biosensors provide a link between biologically defined hazard assessments of metals and standard soil-metal physico-chemical parameters for determining critical metal loadings in soils.