Microgreen Variety Impacts Leaf Surface Persistence of a Human Norovirus Surrogate

Food and Environmental Virology - Human norovirus (HuNoV) is a pathogenic agent that is frequently associated with foodborne disease outbreaks linked to fresh produce. Within microgreen production...     

Reduction of Norovirus Surrogates Alone and in Association with Bacteria on Leaf Lettuce and Tomatoes During Application of Aqueous Ozone

Food and Environmental Virology - Retail foodservice establishments (FSE) frequently utilize washes with sanitizing agents during fresh produce preparation. This study evaluated the efficacy of...     

Leaf-cutter ants with worn mandibles cut half as fast,spend twice the energy,and tend to carry instead of cut

The importance of mechanical wear in the behavioral ecology and energetics of small organisms is an open question. We investigated wear in leaf-cutter ants, Atta cephalotes, because their cutting technique can be imitated and the leaves are the main energy source for the colony. We found that a razor-sharp (50-nm radius) “V-blade” that cuts leaves between the first and second mandibular teeth was dulled (∼10-μm radius) and often nearly worn away on foragers. We found that the force required to cut standard leaves, using mandibles removed from foragers cutting in the wild, varied by a factor of 2.5 with tooth wear, defined as the difference between pupal and actual tooth length. We also found that wear significantly reduced the cutting rate. From the distribution of wear among the cutting foragers, we estimated that the wild colony would have spent 44% less of both energy and time making the observed cuts if the cutters’ mandibles had all been pristine. Finally, wear correlated with behavioral differences—foragers with the most worn 10% of mandibles almost exclusively carried rather than cut. This previously unreported form of task partitioning suggests that eusociality may extend useful lifespans by making it possible to switch tasks as skills decline. We developed a model, assuming that ants do work at a constant rate proportional to their mass, to predict the cutting rate from head width, tooth wear, and force to cut leaves with a scalpel (R = 0.62), and we used this estimate to argue that the partitioning of cutting and carrying was sub-optimal but better than random. Wear’s strong effect on performance may promote wear-avoiding behavior and wear-resistant mandible composition; it may affect leaf selection and worker lifespan and it raises the possibility that wear is a similarly important constraint for many other small organisms.     

Lessons from Developing Methods to Study the Relationship of Energy and Water Savings to Housing Conditions and Resident Health

Abstract

Energy- and water-saving have been promoted as ways of saving money while there has been controversy about the effects (if any) of such measures on resident health. In public housing in the US, energy saving has taken the form of Energy Savings Performance Contracts (ESPC) that pay for renovations out of future savings. In an attempt to test feasibility and pilot research methods we conducted a study of the relationship between gas and water use, exterior heat sensor locations, and basement ventilation with indices developed from resident-reported living conditions and health symptoms in a single housing development. The data sources that we had available to us were not ideal. In particular, we were unable to obtain water use data that coincided in time with our health survey data. Also, we did not have enough surveys in buildings with high water or gas use and had to pool those buildings with other buildings for analysis. Nevertheless, we found several associations between our measures of energy use and resident reports of health symptoms. The associations that we found were generally in the direction that energy and water savings were associated with fewer symptoms and fewer environmental problems. There is a need for studies that obtain better input data but that generally follow the approach we developed.     

Multi-level coordination of climate change adaptation: by national hierarchical steering or by regional network governance?

The institutional structure and public service delivery apparatus required to meet the future effects of climate change already exist in Norway. However, there are huge challenges in coordinating these institutions at different authority levels for climate change adaptation purposes. Based upon a broad case study, this article presents how local actors consider the multi-level coordination of different levels of government and policy sectors to function today, which are the mechanisms that are used and what are the coordination challenges that are identified. Based upon the challenges revealed, this article discusses how best can the government-level institutions be organised for better goal attainment. We argue here that the elected regional level in Norway – the counties – has a huge potential to act as a multi-level coordination actor.     

The use of natural processes for the control of chromium migration

Environmental contamination with ionic chromium has been identified as a problem at numerous Superfund and RCRA Corrective Action sites. In many cases, contamination of groundwater to levels above existing standards or criteria may be a potential problem both for direct consumption of groundwater and for transport of mobile forms of chromium to areas such as basements where it can becontacted. In the environment, chromium occurs in two forms: trivalent and hexavalent. The trivalent form is generally immobile and nontoxic; hexavalent chromium is generally mobile and toxic. This article first presents the extent of the chromium problem, reviews the environmental chemistry literature on chromium, and reviews existing treatment technology for chromium immobilization in the nontoxic trivalent state. Finally, we present a case study where immobilization of chromium occurred through natural processes allowing a modified no-action scenario for site remediation.     

Ecology of juvenile hawksbills (Eretmochelys imbricata) at Buck Island Reef National Monument, US Virgin Islands

Surveys of juvenile hawksbills around Buck Island Reef National Monument, US Virgin Islands from 1994 to 1999 revealed distributional patterns and resulted in a total of 75 individual hawksbill captures from all years; turtles ranged from 23.2 to 77.7 cm curved carapace length (CCL; mean 42.1 ± 12.3 cm SD). Juveniles concentrated where Zoanthid cover was highest. Length of time between recaptures, or presumed minimum site residency, ranged from 59 to 1,396 days (mean 620.8 ± 402.4 days SD). Growth rates for 23 juveniles ranged from 0.0 to 9.5 cm year^?1 (mean 4.1 ± 2.4 cm year^?1SD). Annual mean growth rates were non-monotonic, with the largest mean growth rate occurring in the 30–39 cm CCL size class. Gastric lavages indicated that Zoanthids were the primary food source for hawksbills. These results contribute to our understanding of juvenile hawksbill ecology and serve as a baseline for future studies or inventories of hawksbills in the Caribbean.     

Use of carrion fly iDNA metabarcoding to monitor invasive and native mammals

Severely fragmented habitats increase the risk of extirpation of native mammal populations through isolation, increased edge effects, and predation. Therefore, monitoring the movement of mammal populations through anthropogenically altered landscapes can inform conservation. We used metabarcoding of invertebrate-derived DNA (iDNA) from carrion flies (Calliphoridae and Sarcophagidae) to track mammal populations in the wheat belt of southwestern Australia, where widespread clearing for agriculture has removed most of the native perennial vegetation and replaced it with an agricultural system. We investigated whether the localization of the iDNA signal reflected the predicted distribution of 4 native species—echidna (Tachyglossus aculeatus), numbat (Myrmecobius fasciatus), woylie (Bettongia penicillata), and chuditch (Dasyurus geoffroii)—and 2 non-native, invasive mammal species—fox (Vulpes vulpes) and feral cat (Felis catus). We collected bulk iDNA samples (n = 150 samples from 3428 carrion flies) at 3 time points from 3 conservation reserves and 35 road edges between them. We detected 14 of the 40 mammal species known from the region, including our target species. Most detections of target taxa were in conservation reserves. There were a few detections from road edges. We detected foxes and feral cats throughout the study area, including all conservation reserves. There was a significant difference between the diversity (F_3, 98 = 5.91, p < 0.001) and composition (F_3, 43 = 1.72, p < 0.01) of taxa detections on road edges and conservation reserves. Conservation reserves hosted more native biodiversity than road edges. Our results suggest that the signals from iDNA reflect the known distribution of target mammals in this region. The development of iDNA methods shows promise for future noninvasive monitoring of mammals. With further development, iDNA metabarcoding could inform decision-making related to conservation of endangered taxa, invasive species management, and impacts of habitat fragmentation.     

Evaluation of PFAS treatment technology: Alkaline ozonation

A bench‐scale treatability study was performed to evaluate the effectiveness of alkaline ozonation on removing per‐ and polyfluoroalkyl substances (PFAS) present in groundwater at a former industrial site in Michigan. The study involved testing the PFAS‐impacted groundwater under alkaline ozonating conditions under a range of experimental conditions, including modifying pH, hydrogen peroxide‐to‐ozone molar ratio doses, length of ozonation pretreatment times, and sampling techniques. PFAS‐spiked samples were used to determine if inorganic ions such as fluoride (F^?), sulfate (SO₄^2?), formate (HCOO^?), acetate (CH₃COO^?), and trifluoroacetate (CF₃COO^?) were generated or if there were decreases in total organic fluorine resulting from PFAS treatment. The results from all tests indicate that decreases in PFAS concentrations were due to a combination of removal and destructive mechanisms with enhanced removal under acidic pH ozonation pretreatment conditions. Short‐chain PFAS concentrations increased during the experiments followed by an overall decrease in concentration under continuous alkaline ozonation conditions. Reductions in concentrations in perfluorooctane sulfonic acid of 75–97% were observed. Reductions in concentrations were also observed in other PFAS such as 6:2 FTS, PFHxS, PFOA, and PFNA. To our best knowledge, this is the first time that alkaline ozonation has been performed on PFAS‐impacted water while monitoring a larger suite of PFAS analytes in addition to destruction byproducts. Treatment of PFAS under the conditions discussed in this paper suggests that alkaline ozonation may be a viable remediation option for PFAS‐impacted waters.