Urinary methyl tert-butyl ether and benzene as biomarkers of exposure to urban traffic

Methyl tert-butyl ether (MTBE) and benzene are added to gasoline to improve the combustion process and are found in the urban environment as a consequence of vehicular traffic. Herein we evaluate urinary MTBE (MTBE-U) and benzene (BEN-U) as biomarkers of exposure to urban traffic. Milan urban policemen (130 total) were investigated in May, July, October, and December for a total of 171 work shifts. Personal exposure to airborne benzene and carbon monoxide (CO), and atmospheric data, were measured during the work shift, while personal characteristics were collected by a questionnaire. A time/activity diary was completed by each subject during the work shift. Spot urine samples were obtained for the determination of MTBE-U and BEN-U. Median personal exposure to CO and airborne benzene were 3.3 mg/m(3) and 9.6 μg/m(3), respectively; median urinary levels in end-of-shift (ES) samples were 147 ng/L (MTBE-U) and 207 ng/L (BEN-U). The time spent on traffic duty at crossing was about 40% of work time. Multiple linear regression models, taking into account within-subject correlations, were applied to investigate the role of urban pollution, atmospheric conditions, job variables and personal characteristics on the level of biomarkers. MTBE-U was influenced by the month of sampling and positively correlated to the time spent in traffic guarding, CO exposure and atmospheric pressure, while negatively correlated to wind speed (R(2) for total model 0.63, P<0.001). BEN-U was influenced by the month and smoking habit, and positively correlated to urinary creatinine; moreover, an interaction between CO and smoking was found (R(2)=0.62, P<0.001). These results suggest that MTBE-U is a reliable marker for assessing urban traffic exposure, while BEN-U is determined mainly by personal characteristics.     

Development of a Pollination Service Measurement (PSM) method using potted plant phytometry

The value of pollination to human society is not limited to agricultural production, but also in the sustainability of ecosystems and the services that they provide. Seed set can be used as a comparative measure of pollination effectiveness, with minimum variability expected when other resources are not limiting. Six species of self-incompatible fall asters (Symphyotrichum) were used to evaluate pollination service at 12 sites across a spectrum of expected levels of pollination. Seed set per inflorescence was generally lower at sites with lower pollinator numbers and diversity, although as expected pollinator assemblage characteristics were highly variable within and between sites. However, rankings of sites showed consistency of response across phytometer species and between years; the summed ranks across multiple species appears to have as the greatest value in Pollination Service Measurement (PSM). Abundance, richness, and Shannon diversity of pollinator assemblages were highly autocorrelated and showed variable relationships with seed set depending on plant species and temporal scale of pollinator assemblage assessment. Use of seed set to directly measure pollination service at a site was consistent and cost effective when compared to less certain and more labour-intensive methods of pollinator collection and identification, and shows promise for implementation in pollination monitoring and bioassessment practices.     

Detection and delineation of critical areas using echelons and spatial scan statistics with synoptic cellular data

Geographical surveillance for hotspot detection and delineation has become an important area of investigation both in geospatial ecosystem health and in geospatial public health. In order to find critical areas based on synoptic cellular data, geospatial ecosystem health investigations apply recently discovered echelon tools. In order to find elevated rate areas based on synoptic cellular data, geospatial public health investigations apply recently discovered spatial scan statistic tools. The purpose of this paper is to conceptualize a joint role for these together in the spirit of a cross-disciplinary cross-fertilization to accomplish more effective and efficient geographical surveillance for hotspot detection and delineation, and early warning system.     

Who’s what? Prompt recognition of social status in crayfish

Crayfish are excellent model organisms to study the proximate mechanisms underlying the maintenance of dominance hierarchies in invertebrates. Our aim here was to investigate whether Procambarus clarkii males use social eavesdropping to discriminate dominant from subordinate crayfish. To this end, we conducted an experiment composed of a “passive” and an “active” phase. In the passive phase, “focal” individuals were allowed (treatment 1) or not (treatment 2) to see and smell two size-matched crayfish fighting while, in the subsequent active phase, they were allowed to freely interact with the fighting dyad. None of the recorded variables showed any significant difference between the two treatments, but, invariably, focal individuals were able to promptly discriminate dominant from subordinate crayfish. This study provides evidence that male crayfish recognize the social status of a conspecific without the need of direct or indirect experience with it and avoid dominants—and thus dangerous opponents—by means of a badge of status. A form of “winner and loser effects” could also contribute to the structuring of dominance/subordinate relationships. The implication of these results in understanding the maintenance of dominance hierarchies in invertebrates are discussed and compared with findings previously achieved in the context of mate choice by P. clarkii females, who do appear to use eavesdropping to identify dominants and subordinates.     

Expanding Kenya's protected areas under the Convention on Biological Diversity to maximize coverage of plant diversity

Biodiversity is highly valuable and critically threatened by anthropogenic degradation of the natural environment. In response, governments have pledged enhanced protected‐area coverage, which requires scarce biological data to identify conservation priorities. To assist this effort, we mapped conservation priorities in Kenya based on maximizing alpha (species richness) and beta diversity (species turnover) of plant communities while minimizing economic costs. We used plant‐cover percentages from vegetation surveys of over 2000 plots to build separate models for each type of diversity. Opportunity and management costs were based on literature data and interviews with conservation organizations. Species richness was predicted to be highest in a belt from Lake Turkana through Mount Kenya and in a belt parallel to the coast, and species turnover was predicted to be highest in western Kenya and along the coast. Our results suggest the expanding reserve network should focus on the coast and northeastern provinces of Kenya, where new biological surveys would also fill biological data gaps. Meeting the Convention on Biological Diversity target of 17% terrestrial coverage by 2020 would increase representation of Kenya's plant communities by 75%. However, this would require about 50 times more funds than Kenya has received thus far from the Global Environment Facility.     

Historical perspectives on contemporary human–environment dynamics in southeast Africa

The human communities and ecosystems of island and coastal southeast Africa face significant and linked ecological threats. Socioecological conditions of concern to communities, governments, nongovernmental organizations, and researchers include declining agricultural productivity, deforestation, introductions of non-native flora and fauna, coastal erosion and sedimentation, damage to marine environments, illegal fishing, overfishing, waste pollution, salinization of freshwater supplies, and rising energy demands, among others. Human–environment challenges are connected to longer, often ignored, histories of social and ecological dynamics in the region. We argue that these challenges are more effectively understood and addressed within a longer-term historical ecology framework. We reviewed cases from Madagascar, coastal Kenya, and the Zanzibar Archipelago of fisheries, deforestation, and management of human waste to encourage increased engagement among historical ecologists, conservation scientists, and policy makers. These case studies demonstrate that by widening the types and time depths of data sets we used to investigate and address current socioecological challenges, our interpretations of their causes and strategies for their mitigation varied significantly.     

Enhancement of conservation knowledge through increased access to botanical information

Herbarium specimens are increasingly recognized as an important resource for conservation science and virtual herbaria are making specimens freely available to a wider range of users than ever before. Few virtual herbaria are designed with conservation use as a primary driver. Exceptionally, Brazil's Reflora Virtual Herbarium (RVH) was created to increase knowledge and conservation of the Brazilian flora. The RVH is closely integrated with the Flora of Brazil 2020 platform on which Brazil's new national Flora is under construction. Both resources are accessible via the Reflora home page and thousands of users move seamlessly between these Reflora resources. To understand how the Reflora resources are currently used and their impact on conservation science, we conducted a literature review and an online survey. We searched for publications of studies in which Reflora resources were used and publications resulting from Brazilian researchers who were part of Reflora's research and mobility program. The survey contained multiple choice questions and questions that required a written response. We targeted Reflora webpage visitors with the survey to capture a wider range of Reflora users than the literature review. Reflora resources were used for a variety of conservation-relevant purposes. Half the 806 scientific publications in which Reflora was cited and 81% of the 1069 survey respondents accessing Reflora resources mentioned conservation-relevant research outputs. Most conservation-relevant uses of the Reflora resources in scientific publications were research rather than implementation focused. The survey of Reflora users showed conservation uses and impacts of virtual herbaria were more numerous and diverse than the uses captured in the literature review. Virtual herbaria are vital resources for conservation science, but they must document use and impacts more comprehensively to ensure sustainability.     

Dissipation of terbuthylazine,metolachlor, and mesotrione in soils with contrasting texture

ABSTRACT

This study evaluates the dissipation of terbuthylazine, metolachlor, and mesotrione at different depths in soils with contrasting texture. The field trial was conducted at the Padua University Experimental Farm, north-east Italy. The persistence of three herbicides was studied in three different soil textures (clay soil, sandy soil, and loamy soil) at two depths (0–5 and 5–15 cm). Soil organic carbon content was highest in the clay (1.10%) followed by loam (0.67%) and sandy soil (0.24%); the pH of soils was sub-alkaline. Terbuthylazine, metolachlor, and mesotrione were applied on maize as a formulated product (Lumax®) at a dose of 3.5 L ha^?1. Their dissipation in the treated plots was followed for 2 months after application. The concentrations of herbicides were analyzed by liquid chromatography-mass spectrometry. The dissipation of terbuthylazine, metolachlor, and mesotrione could be described by a pseudo first-order kinetics. Terbuthylazine showed the highest DT50, followed by metolachlor and mesotrione. Considering the tested soil, the highest DT50 value was found in clay soil for terbuthylazine and metolachlor, whereas for mesotrione there was no difference among soils. Significant differences were found between the two soil depths for terbuthylazine and metolachlor, whereas none were found for mesotrione. These results suggest that soil texture and depth have a strong influence on the dissipation of terbuthylazine and metolachlor, whereas no influence was observed on mesotrione because of its chemical and physical properties.     

The trace element content of top-soil and wild edible mushroom samples collected in Tuscany, Italy

The amount of the trace elements As, Ba, Cd, Cr, Cu, Hg, Li, Mn, Ni, Pb, Rb, Se, Sr, and Zn was measured in top soils and edible mushrooms, Boletus edulis, Macrolepiota procera, collected at five distinct green microhabitats inside the Lucca province, North-Central Italy (years 2008–2009). Results showed a top soil element content within the Italian statutory limits. Concerning the amount of mushroom elements, we observed significant species-differences obtaining higher levels of Ni, Rb, and Se in B. edulis or As, Pb, Cu in M. procera. Bioaccumulation factors (BCFs: element in mushroom/element in soil) resulted species-dependent and element-selective: in particular, B. edulis preferentially accumulated Se (BCFs varying from 14 to 153), while M. procera mainly concentrated Cu (BCFs varying from 5 to 15). As well, both species displayed between-site BCF differences. By a multivariate principal component approach, cluster analysis (CA), we could resolve two main clusters of soil element composition, corresponding to the most ecologically divergent sites. Besides, CA showed no cluster relating to element contents of B. edulis at the different collection sites, while a separation in groups was found for M. procera composition with respect to harvesting locations, suggesting uptake systems, in this saprotrophic species, sensitive to microhabitat. Regarding consumer safety, Cd, Hg, Pb levels resulted sometime relevant in present samples, never reaching values from current literature on mushrooms collected in urban-polluted areas. Our findings encourage a deeper assessment of the molecular mechanisms of metal intake by edible mushrooms, encompassing genetic biochemical and geo-ecological variables, with particular awareness to element bioavailability in soils and fungi.     

Movement and environmental preferences of Greenland sharks (<Emphasis Type="Italic">Somniosus microcephalus</Emphasis>) electronically tagged in the St. Lawrence Estuary,Canada

Three Greenland sharks (Somniosus microcephalus) were tagged with electronic tags, in Baie St. Pancrace, St. Lawrence Estuary, Quebec, Canada. One shark was tagged on 23 July 2004, with an acoustic telemetry tag. Two sharks were each tagged with a pop-up satellite archival tag (PSAT) on 27 August 2004. Two of the sharks remained in or close to the bay, one for 47 days and the other for at least 66 days. The third shark left the bay immediately after tagging on 27 August 2004. This shark entered the main channel of the St. Lawrence Estuary, and had moved 114.9 km upstream by 1 November 2004 when the tag reported to ARGOS satellites. The tags provided a total of 179 days of data on the movement and environmental preferences of Greenland sharks in the St. Lawrence Estuary. Sharks that reported depth and ambient water temperature data from the bay showed significant diel differences in depth preferences and corresponding ambient temperatures. The sharks remained near the bottom of the water column during the day and displayed increased vertical movements at night. The shark that resided in the main channel did not show this pattern, but generally remained at depths between 325 and 352 m. Sharks in the bay experienced water temperatures that ranged from −1.1 to 8.6°C at depths from 0 to 67 m. In the main channel the shark experienced temperatures that ranged from 1.0 to 5.4°C at depths from 132 to 352 m. This is the first report of numerous Greenland sharks inhabiting shallow near shore bays during summer and autumn.