Temporal and spatial distribution of three supralittoral amphipod species on a sandy beach of central Italy

The distribution of three talitrid species—Talitrus saltator (Montagu, 1808), Orchestia gammarella (Pallas, 1766), Platorchestia platensis (Kroyer, 1845)—in the beach-dune system at the mouth of the Mignone River (central Italy) was analysed. It was related to the variations of the following abiotic factors: temperature, penetrability, pH, conductivity and moisture of the sediment. The beach-dune system is influenced by human impact and natural erosion. All species showed a maximum capture frequency in November, while the abundance decreased to a minimum in July. T. saltator was dominant on the beach, the other two along the riverbank. Juveniles were abundant along the riverbank; they were most abundant in November and almost disappeared in summer. T. saltator was more abundant near the waterline during the hottest months and occupied the inner beach in winter, with occasional presences on the dune. Regression analysis between the abiotic factors and species abundance showed a positive relationship with pH and temperature for T. saltator, while O. gammarella was negatively related to pH. Canonical correspondence analysis (CCA) showed that penetrability, moisture and temperature had the greatest influence on the species. T. saltator was almost entirely confined to the beach transects and strongly associated with penetrability, temperature and pH values. O. gammarella was mostly associated with the riverbank and P. platensis only found there and on a nearby pool. The two species were mainly influenced by moisture and variations in grain sizes of the sediment.     

Alternative mating tactics in males of Polistes dominulus (Hymenoptera: Vespidae)

Summary In summer, males of Polistes dominulus form large aggregations at sunny landmarks. We identified two size-correlated behavioural categories: residents (R) and transient (T). R males, which constitute 20%–25% of the total population, are larger than T males, territorial, aggressive, and more site-faithful, while T males range more widely, are non-aggressive, and show little site tenacity. Field and laboratory data suggest that R males have an advantage in mating, particularly if they engage in frequent flights while on their territories. These alternative mating tactics within the same population are combined with behavioural flexibility in some individuals, which switch from one option to the other.     

Odour impact assessment by means of dynamic olfactometry,dispersion modelling and social participation

This work discusses how it is possible to assess odour impact in presence of multiple similar sources by illustrating a case study. The study was conducted on an area of northern Italy comprising three small municipalities where four rendering plants are located near to each other. Based on the emission data resulting from olfactometric surveys conducted in different periods of the year the overall odour emission rate emitted by each plant were evaluated, showing that the major contributor to the odour impact on the territory was plant 2. These data were linked with meteorological and orographical data in order to evaluate odour dispersion with a model (Calpuff). The results of the odour dispersion modelling confirmed the outcomes of the olfactometric survey and they were further validated through a “questioning” survey, conducted with the aim of involving the population by means of questionnaires for reporting the perceived odour episodes, which showed a good correspondence (86.5%) between odour perceptions and simulated odour immissions.     

Examining the “evolution of increased competitive ability” hypothesis in response to parasites and pathogens in the invasive paper wasp Polistes dominula

Successful invaders often become established in new ranges by outcompeting native species. The “evolution of increased competitive ability” hypothesis predicts that invasive species are subjected to less predation and parasitization than sympatric native species, and thus can allocate resources from defence and immunity to growth and fecundity, thereby achieving higher fitness. In this study, we examined whether American invasive Polistes dominula paper wasps have reduced immunocompetence. To explore this scenario, we tested their susceptibility towards parasites and pathogens at both the individual (immune defence) and colony levels, i.e. hygienic behaviour (removal of diseased individuals by nestmates). First, we examined the response to the specific coevolved parasite Xenos vesparum (lost after invasion) in terms of individual host susceptibility and hygienic behaviour. Second, we explored the response against general pathogens by quantifying the bacterial clearance in individual wasps after a challenge with Escherichia coli and hygienic behaviour after a challenge with the fungus Beauveria bassiana. Our results show that American invasive P. dominula have a higher response against X. vesparum at the colony level, but at the individual level their susceptibility is not significantly different from conspecifics of the native range. On the other hand, invasive P. dominula display lower response after a challenge with general pathogens at both the individual and colony levels. While supporting the hypothesis of a reduction of immunocompetence towards general pathogens in invasive species, these findings also suggest that the response against coevolved parasites might follow different evolutionary pathways which are not always easily predictable.     

X-linked pyruvate dehydrogenase complex deficiency due to a novel PDHA1 variant associated with structural brain abnormalities in a fetus

We report a case of pyruvate dehydrogenase E1 alpha subunit deficiency associated with a novel hemizygous PDHA1 variant presenting prenatally as multiple structural brain abnormalities in a male fetus. A healthy Finnish couple was initially referred to the Fetomaternal Medical Center because of suspected fetal choroid plexus cyst at 11 + 2 weeks of pregnancy. At 20 + 0 weeks, multiple abnormalities were observed with ultrasound including narrow thorax, slightly enlarged heart, hypoplastic cerebellum, absent cerebellar vermis and ventriculomegaly. Autopsy and genetic analyses were performed after the termination of pregnancy. The findings of macroscopic examination included cleft palate, abnormally overlapping position of fingers and toes and dysmorphic facial features. Neuropathological examination confirmed the absence of corpus callosum, cerebellar hypoplasia and ventriculomegaly. Nodular neuronal heterotopia was also observed. Trio exome sequencing revealed a novel hemizygous de novo variant c.1144C>T p.(Gln382*) in the PDHA1 gene, classified as likely pathogenic. We suggest that inherited metabolic disorders should be kept in mind as differential diagnoses in fetuses with structural brain abnormalities.