A High Performance Liquid Chromatographic Determination of Free Amino Acids in Natural Waters in the Picomolar (M X 10-12) Range Suitable for Shipboard Use

A reliable method for the determination of amino acids, suitable for routine shipboard use, is presented. Using reverse-phase high-performance liquid chromatography with computer-controlled gradient elution optimization, the amino acids commonly found in sea-water were clearly resolved and determined at the picomole level. A procedure was also developed to enable repeat injections of sea water samples to be made without deterioration of column material or performance. Thus the analysis was made more operator-independent and consequently more suitable for use at sea.     

Back injury research: Have we overlooked something?

Most back injury research and the safety programs based on its findings have assumed disc and vertebral failure to be the result of compression and/or shear forces. This paper draws on selected references along with a knowledge of how materials fail under simple and combined stresses to postulate alternative failure mechanisms which, if proven, could lead to improvements in back injury prevention programs.     

Croppers to livestock keepers: livelihood transitions to 2050 in Africa due to climate change

The impacts of climate change are expected to be generally detrimental for agriculture in many parts of Africa. Overall, warming and drying may reduce crop yields by 10–20% to 2050, but there are places where losses are likely to be much more severe. Increasing frequencies of heat stress, drought and flooding events will result in yet further deleterious effects on crop and livestock productivity. There will be places in the coming decades where the livelihood strategies of rural people may need to change, to preserve food security and provide income-generating options. These are likely to include areas of Africa that are already marginal for crop production; as these become increasingly marginal, then livestock may provide an alternative to cropping. We carried out some analysis to identify areas in sub-Saharan Africa where such transitions might occur. For the currently cropped areas (which already include the highland areas where cropping intensity may increase in the future), we estimated probabilities of failed seasons for current climate conditions, and compared these with estimates obtained for future climate conditions in 2050, using downscaled climate model output for a higher and a lower greenhouse-gas emission scenario. Transition zones can be identified where the increased probabilities of failed seasons may induce shifts from cropping to increased dependence on livestock. These zones are characterised in terms of existing agricultural system, current livestock densities, and levels of poverty. The analysis provides further evidence that climate change impacts in the marginal cropping lands may be severe, where poverty rates are already high. Results also suggest that those likely to be more affected are already more poor, on average. We discuss the implications of these results in a research-for-development targeting context that is likely to see the poor disproportionately and negatively affected by climate change.     

Stakeholder interaction within the ERICA Integrated Approach

Within the ERICA project, stakeholder involvement has been addressed within three main areas: generic interactions throughout the project, specific consultation by means of attendance to events and considerations as part of the ERICA Integrated Approach and Assessment Tool. The word stakeholders meant namely any individual or group who may be affected by or have an interest in an issue, and to include experts, lay-people and the public. An End-Users-Group (EUG) was set up to facilitate the two-way dialogue between the ERICA Consortium and stakeholders. The ERICA EUG consisted of representatives of 60 organisations ranging from regulatory bodies, national advisory bodies, academia, non-governmental organisations, industry, consultants and inter-governmental organisations. Stakeholder interaction was included from the very start of the project. Inputs from the EUG were recorded and in most instances incorporated within the development of the project and thus influenced and helped to shape some of the ERICA deliverables.     

Characterization of organics in aqueous effluents of coal conversion processes

High-resolution liquid chromatography is being applied to the characterization of refractory organic compounds present in coal conversion streams at concentrations as low as a few micrograms per liter. The chromatographic system, which was previously developed for the analysis of the molecular biochemical constituents in human body fluids, is capable of analyzing for compounds that are u.v.-absorbing and/or oxidizable with sulfatoceric acid. Aqueous samples from various coal-liquefaction experiments have been collected, concentrated when necessary, and chromatographed. The chromatographic fractions have then been subjected to a multiple-analytical identification procedure utilizing, in sequence, UV-spectrometry, gas chromatography, and mass spectrometry. With this procedure, 18 organics were identified and 15 were quantified in the effluent from the product scrubber of a bench-scale hydrocarbonization unit. In addition, numerous unknown constituents have been characterized with respect to gas chromatographic and mass spectrometric properties.     

Background exposure rates of terrestrial wildlife in England and Wales

It has been suggested that, when assessing radiation impacts on non-human biota, estimated dose rates due to anthropogenically released radionuclides should be put in context by comparison to dose rates from natural background radiation. In order to make these comparisons, we need data on the activity concentrations of naturally occurring radionuclides in environmental media and organisms of interest. This paper presents the results of a study to determine the exposure of terrestrial organisms in England and Wales to naturally occurring radionuclides, specifically (40)K, (238)U series and (232)Th series radionuclides. Whole-body activity concentrations for the reference animals and plants (RAPs) as proposed by the ICRP have been collated from literature review, data archives and a targeted sampling campaign. Data specifically for the proposed RAP are sparse. Soil activity concentrations have been derived from an extensive geochemical survey of the UK. Unweighted and weighted absorbed dose rates were estimated using the ERICA Tool. Mean total weighted whole-body absorbed dose rates estimated for the selected terrestrial organisms was in the range 6.9 x 10(-2) to 6.1 x 10(-1) microGy h(-1).     

Change in the quantity and acute toxicity of pesticides sold in South African crop sectors, 1994–1999

BackgroundSouth African pesticide market sales data, for two years, 1994 and 1999, were audited to identify change in total and per hectare mass sold and acute toxicity indicator (ATI) (kg sold/rat oral LD50) in the grape, pome, stone fruit, potato and wheat sectors.ResultsTotal pesticide sales (62%), amount per hectare (42%) and number of active ingredients (23%) increased in 1999 compared to 1994 with the grape fruit sector, the most significant contributor over the two years. Total (14%) and per hectare ATI (19%) decreased in 1999, but not substantially with the potato sector the most significant contributor.ConclusionsToxic pesticides were still used in 1999 which highlights a need to develop alternative agricultural and non-chemical pest control methods that reduce usage of pesticides.